Fetal hemoglobin in umbilical cord blood in preeclamptic and normotensive pregnancies: A cross-sectional comparative study

Abstract A comparison has been made of the determination of fetal hemoglobin in human umbilical cord blood by column chromatography and alkali denaturation. A careful study has also been made of the variables that control the accuracy and precision of the methods. Minor modification has led to much improved control of the 1-minute alkali denaturation procedure. The percentage of fetal hemoglobin in the umiblical cord blood of full term infants has been found to cover a far narrower range than is commonly reported. By chromatography, the average is 85.5 per cent with a range from 79 to 91 per cent that includes more than 95 per cent of normal full term infants. By alkali denaturation, the average is 74.0 per cent with a range from 63 to 87 per cent. Possible correlations with several clinical parameters have been examined. The highest correlation by both methods of determination occurred in the group of 12 samples from infants with a duration of gestation less than 37 weeks. In this group the linear correlation with weight was greater than 0.6. The precision and accuracy of the chromatographic method recommend it in the study of such subjects as prematurity, twinning, dysmaturity, intrauterine growth retardation, and infants of diabetic mothers.

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Lin28B Increases Fetal Hemoglobin and Regulates BCL11A Expression in Human Erythroid Tissues

Blood ◽

10.1182/blood.v120.21.827.827 ◽

2012 ◽

Vol 120 (21) ◽

pp. 827-827 ◽

Cited By ~ 1

Author(s):

Jaira F. de Vasconcellos ◽

Y. Terry Lee ◽

Joan Yuan ◽

Colleen Byrnes ◽

Seung-Jae Noh ◽

...

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Fetal Hemoglobin ◽

Rt Pcr ◽

Gamma Globin ◽

Hemoglobin Switching ◽

Expression Control ◽

Microrna Family ◽

Cd34 Cells

Abstract Abstract 827 The highly-conserved Lin28 genes regulate cellular metabolism as well as the timing of developmental events and cell fates in multicellular organisms. Lin28 protein acts primarily by negatively regulating biogenesis of let-7 RNA, a microRNA family whose targets include growth-related signaling and transcription factor proteins. Published studies showed significantly increased expression of let-7 in purified adult blood reticulocytes compared to umbilical cord blood reticulocytes (1). This pattern correlates inversely with Lin28B expression. While present in the fetal liver and umbilical cord blood, Lin28B decreased to undetectable levels in adult bone marrow (2). Based upon the association of human ontogeny with hemoglobin switching, Lin28 was explored to identify novel mechanisms for hemoglobin regulation that may be useful for therapeutic application among patients with thalassemia or other hemoglobinopathies. To study the effects of Lin28B upon erythropoiesis and hemoglobin, ectopic expression of Lin28B was accomplished using retroviral transduction of human CD34+ cells cultivated ex vivo in erythropoietin-supplemented, serum-free cultures for 21 days. All experiments were performed in triplicate using cells from three separate adult volunteers. Lin28B over-expression (Lin28B-OE) was confirmed by Q-RT-PCR (control: 0.14 ± 0.37 copies/ng, Lin28B-OE: 1.8E+04 ± 353.8 copies/ng, p=0.01). Western analyses confirmed protein expression, and confocal microscopy revealed Lin28B predominantly in the cytoplasm of the transduced cells. Proliferation, maturation and morphology assays revealed that Lin28B-OE did not inhibit erythropoiesis when compared to control (empty vector) transductions. Terminal maturation with loss of CD71 from the erythroblast surface and enucleation by culture day 21 was detected in the control and Lin28B-OE samples. Expression levels of globin genes were evaluated upon Lin28B-OE by Q-RT-PCR. Lin28B-OE enhances gamma-globin mRNA expression (control: 5.14E+06 ± 2.6E+06 copies/ng, Lin28B-OE: 1.81E+07 ± 5.82E+06 copies/ng, p=0.038). Protein analysis confirmed the increased expression of gamma-globin. Fetal hemoglobin (HbF) levels were also increased in the Lin28B-OE cultures (control: 5.82 ± 4.54%, Lin28B-OE: 33.63 ± 9.38%; p=0.011). The increased HbF expression was maintained throughout differentiation including enucleated populations of culture-generated erythrocytes. Possible mechanism(s) for the increased expression of HbF caused by Lin28B-OE were investigated. Q-RT-PCR analyses demonstrated suppression of the let-7 microRNA family with greater-than 70% reductions of let-7a, let-7b, let-7c, let-7d, let-7e, let-7f-2, let-7g and let-7i. Expression patterns of several transcription factors including BCL11A, KLF1, SOX6 and GATA1 were explored. No major changes were detected with the exception of BCL11A. Lin28B-OE caused a 65% reduction in BCL11A expression (control: 3.07E+03 ± 1.5E+02 copies/ng, Lin28B-OE: 1.07E+03 ± 18 copies/ng; p=0.02). Western blot analyses of Lin28B-OE showed a consistent reduction of BCL11A protein. By comparison with Lin28B-OE, separately performed studies of BCL11A knockdown in adult CD34+ cells produced comparable increases in gamma-globin expression, but Lin28B expression in those cells was not affected. In addition to a more general role in development and metabolism, these experimental results suggest that Lin28B increases fetal hemoglobin and regulates BCL11A in human erythroblasts. Lin28B is thus identified as the first defined link between the regulation of a developmental clock and hemoglobin switching in humans. Disclosures: No relevant conflicts of interest to declare.

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IL-6 and IL-10 levels in the umbilical cord blood of newborns with a history of crack/cocaine exposure in utero: a comparative study

Trends in Psychiatry and Psychotherapy ◽

10.1590/2237-6089-2015-0081 ◽

2016 ◽

Vol 38 (1) ◽

pp. 40-49 ◽

Cited By ~ 3

Author(s):

Victor Mardini ◽

Luis Augusto Rohde ◽

Keila Maria Mendes Ceresér ◽

Carolina de Moura Gubert ◽

Emily Galvão da Silva ◽

...

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Crack Cocaine ◽

In Utero ◽

Childhood And Adolescence ◽

Cocaine Exposure ◽

Cross Sectional ◽

History Of ◽

Spearman Test

Introduction Prenatal cocaine exposure (PCE) is associated with neurobehavioral problems during childhood and adolescence. Early activation of the inflammatory response may contribute to such changes. Our aim was to compare inflammatory markers (IL-6 and IL-10) both in umbilical cord blood and in maternal peripheral blood at delivery between newborns with history of crack/cocaine exposure in utero and non-exposed newborns. Methods In this cross-sectional study, 57 newborns with a history of crack/cocaine exposure in utero (EN) and 99 non-exposed newborns (NEN) were compared for IL-6 and IL-10 levels. Sociodemographic and perinatal data, maternal psychopathology, consumption of nicotine and other substances were systematically collected in cases and controls. Results After adjusting for potential confounders, mean IL-6 was significantly higher in EN than in NEN (10,208.54, 95% confidence interval [95%CI] 1,328.54-19,088.55 vs. 2,323.03, 95%CI 1,484.64-3,161.21; p = 0.007; generalized linear model [GLM]). Mean IL-10 was also significantly higher in EN than in NEN (432.22, 95%CI 51.44-812.88 vs. 75.52, 95%CI 5.64-145.39, p = 0.014; GLM). Adjusted postpartum measures of IL-6 were significantly higher in mothers with a history of crack/cocaine use (25,160.05, 95%CI 10,958.15-39,361.99 vs. 8,902.14, 95%CI 5,774.97-12,029.32; p = 0.007; GLM), with no significant differences for IL-10. There was no correlation between maternal and neonatal cytokine levels (Spearman test, p ≥ 0.28 for all measures). Conclusions IL-6 and IL-10 might be early biomarkers of PCE in newborns. These findings could help to elucidate neurobiological pathways underlying neurodevelopmental changes and broaden the range of possibilities for early intervention.

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Cell-Based Regenerative Strategies for Treatment of Diabetic Skin Wounds, a Comparative Study between Human Umbilical Cord Blood-Mononuclear Cells and Calves' Blood Haemodialysate

PLoS ONE ◽

10.1371/journal.pone.0089853 ◽

2014 ◽

Vol 9 (3) ◽

pp. e89853 ◽

Cited By ~ 2

Author(s):

Hala O. El-Mesallamy ◽

Mohamed R. Diab ◽

Nadia M. Hamdy ◽

Sarah M. Dardir

Keyword(s):

Comparative Study ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Mononuclear Cells ◽

Human Umbilical Cord Blood ◽

Human Umbilical Cord ◽

Skin Wounds ◽

Blood Mononuclear Cells ◽

Cord Blood Mononuclear Cells

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Knowledge, Awareness, and Attitudes toward Umbilical Cord Blood Biobanking

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2021.6464 ◽

2021 ◽

Vol 9 (E) ◽

pp. 702-709

Author(s):

Nevenka Kregar Velikonja ◽

Karmen Erjavec ◽

Miomir Knežević

Keyword(s):

Stem Cells ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Online Survey ◽

Research Knowledge ◽

Alternative Source ◽

Cross Sectional ◽

Hematopoietic Stem ◽

Academic Background

BACKGROUND: Umbilical Cord Blood (UCB) stem cells are a non-invasive, effective alternative source of hematopoietic stem cells for the treatment of a variety of diseases. AIM: The aim of the study was to research knowledge, awareness, and attitudes of the general public and health professionals regarding the UCB storage. MATERIALS AND METHODS: A cross-sectional study was conducted with an online survey (n = 408) using the snowball method. RESULTS: A majority of respondents declared that they were not aware of UCB banking; however, people with an academic background in healthcare have a greater awareness of UCB banking. A subjective assessment found that their knowledge of UCB banking is better than that of non-professionals, however, they exhibited better knowledge and understanding of UCB use and banking in only a few aspects. People with an academic background in healthcare have a more polarized attitude toward cord blood banking and a higher percentage are unwilling to pay for UCB banking services. However, their preferences regarding public/private UCB banking do not differ significantly from those of non-professionals. CONCLUSION: Here, we show that there is not much difference between the laic and professional categories in terms of knowledge about the specific purpose and characteristics of UCB storage.

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Assessment of oxidative damage and enzymatic antioxidant system activity on the umbilical cord blood and saliva from preterm newborns with risk factors for early-onset neonatal sepsis

Revista da Associação Médica Brasileira ◽

10.1590/1806-9282.64.10.888 ◽

2018 ◽

Vol 64 (10) ◽

pp. 888-895 ◽

Cited By ~ 1

Author(s):

Fábio Gonçalves Coutinho ◽

Edna Maria de Albuquerque Diniz ◽

Ingrid Kandler ◽

Marco Antônio Cianciarullo ◽

Natália Rodrigues dos Santos

Keyword(s):

Risk Factors ◽

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Neonatal Sepsis ◽

Early Onset ◽

Umbilical Vein ◽

Statistical Significance ◽

Cross Sectional ◽

Significant Difference

SUMMARY BACKGROUND: To determine the concentration of the Lipid Peroxidation Marker: Malondialdehyde (MDA), and Antioxidant Markers: Superoxide Dismutase (SOD), Glutathione Peroxidase (GPX), Catalase (CAL) in umbilical cord blood and in unstimulated saliva in the first 24 and 48 hours of life in the PTNB of mothers with and without risk factors for early-onset neonatal sepsis. METHODS: Cross-sectional study with the signing of informed consent by the pregnant women and application of a standard questionnaire classifying the PTNB in Group 1 or 2. RESULTS: Twenty-one PTNB were studied. Regarding gender, birth weight, need for oxygen, use of phototherapy, diagnosis of assumed sepsis, presence of fetal distress, number of pregnancies, type of delivery, use of corticosteroids, premature rupture of membranes, maternal fever, chorioamnionitis, APGAR at the 5th and 10th minute of life. Statistical analysis was performed with the Mann-Whitney test (p = 0.019) on the GPX variable of umbilical cord blood in the group of mothers with risk factors for early-onset neonatal sepsis. There was no statistical difference in the MDA, SOD, and CAT variables of the group with risk factors and in any variable of the group without risk factors. CONCLUSION: There was an increase of the GPX concentration in the blood from the umbilical vein in the group with risk factors for early-onset neonatal sepsis. There was no statistical significance in the comparison of saliva and umbilical cord blood. There was no statistically significant difference in MDA, SOD, CAT.

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UMBILICAL CORD BLOOD HEMATOLOGY IN RELATION WITH MATERNAL ANEMIA: A PRELIMINARY STUDY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i10.26789 ◽

2018 ◽

Vol 11 (10) ◽

pp. 403

Author(s):

Rumi Debbarma ◽

Pankaj Pankaj ◽

M. Anita Devi

Keyword(s):

Cord Blood ◽

Umbilical Cord ◽

Umbilical Cord Blood ◽

Hemoglobin Concentration ◽

Blood Parameters ◽

Total Leukocyte Count ◽

Cross Sectional ◽

Maternal Anemia ◽

Distribution Width ◽

Preliminary Study

Objectives: The aim of this study was to observe any umbilical cord blood hematological changes in relation to maternal anemia.Methods: We conducted a cross-sectional study on 220 neonates and their mothers from August 1, 2015, to July 31, 2016, in collaboration with the Department of Obstetrics and Gynaecology, Regional Institute of Medical Sciences, Imphal, India. Immediately after vaginal delivery of baby, umbilical cord was clamped and blood was collected in EDTA vials and analyzed using automated hematoanalyzer.Results: Neonates of non-anemic mothers had significantly higher level of hemoglobin concentration than neonates of anemic mothers. Total leukocyte count (TLC) was slightly higher in neonates of non-anemic mothers as compared to neonates of anemic mothers. Platelet count was slightly higher in neonates of anemic mothers as compared to non-anemic mothers, but platelet crit, mean platelet volume, and platelet cell distribution width were slightly higher in neonates of non-anemic mothers than neonates anemic mothers.Conclusions: It was a preliminary study. A similar type of the study must be conducted in future to comprehend the effect of various other factors affecting delivery such as maternal anemia, diet, and environment on blood parameters of newborns.

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