scholarly journals Sequence analysis of the rifampicin resistance determining region (RRDR) of rpoB gene in multidrug resistance confirmed and newly diagnosed tuberculosis patients of Punjab, Pakistan

PLoS ONE ◽  
2017 ◽  
Vol 12 (8) ◽  
pp. e0183363 ◽  
Author(s):  
Salma Hameed ◽  
Kartyk Moganeradj ◽  
Nasir Mahmood ◽  
Timothy D. McHugh ◽  
Muhammad Nawaz Chaudhry ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Sequence Analysis ◽  
Rpob Gene ◽  
Rifampicin Resistance ◽  
Newly Diagnosed ◽  
Tuberculosis Patients

scholarly journals Multidrug resistance and demography of newly diagnosed tuberculosis patients in Cross River State, Nigeria

2013 ◽  
Vol 2 (2) ◽  
pp. 89-93 ◽  
Author(s):  
Benjamin Thumamo Pokam ◽  
Anne E. Asuquo ◽  
Lydia N. Abia-Bassey ◽  
Mandor B. Idasa ◽  
Nse O. Umoh ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Newly Diagnosed ◽  
Tuberculosis Patients ◽  
Cross River ◽  
Cross River State

scholarly journals Study of rifampicin resistance among newly diagnosed pulmonary tuberculosis patients with type 2 diabetes mellitus: a prospective observational study

2021 ◽  
Vol 9 (7) ◽  
pp. 2035
Author(s):  
Vipul Kumar ◽  
Jyoti Yadav ◽  
Aparna Parmar ◽  
Ritu Aggarwal ◽  
K. B. Gupta
Keyword(s):  
Diabetes Mellitus ◽  
Pulmonary Tuberculosis ◽  
Prospective Observational Study ◽  
Rifampicin Resistance ◽  
Age Group ◽  
Newly Diagnosed ◽  
Tuberculosis Patients ◽  
Mdr Tb ◽  
A Prospective Study

Background: Rifampicin (RIF) resistance in new cases of pulmonary tuberculosis is a matter of concern. Diabetes Mellitus triples the risk of developing tuberculosis. Early detection of TB and its resistance status in diabetics can help in improving the care and treatment outcomes of both diseases.Methods: It was a prospective study conducted from February 2019 to March 2020 in PGIMS, Rohtak on 50 patients of DM with newly diagnosed Pulmonary TB. Rifampicin resistance was detected by CBNAAT on sputum, induced sputum and BAL samples. Results: Mean age of study subjects was 51.24±10.421 (in years) with M: F ratio of 3:1 and maximum patients in 50-59 age group. The average BMI in patients was 22.49±2.42 kg/m2. The most common presenting complaint was cough (92%) followed by fever (68%). Mean serum HBA1c was 9.66±2.24 and mean FBS and PPBS was 195.81±59.08 and 302.02±99.01 mg/dl respectively. Out of 36 cases who gave sputum, 29 (80.55%) were detected rifampicin sensitive and 7 (19.44%) were rifampicin resistant whereas out of 11 who were induced, 9 (81.8%) were rifampicin sensitive and 2(18.18%) were rifampicin resistant. Out of 3 cases detected by BAL CBNAAT, 2 (66.6%) were rifampicin sensitive and 1 (33.33%) was rifampicin resistant. Overall, 10(20%) patients were detected Rifampicin resistant by CBNAAT.Conclusions: We found that TB-DM patients had a higher proportion of drug resistance (20%), so DM should be considered as an independent risk factor for MDR-TB and effective measures are required for early diagnosis of MDR-TB.

Emergence of Heteroresistance Mycobacterium Tuberculosis in Saudi Arabia

2020 ◽  
Vol 20 (4) ◽  
pp. 491-494 ◽  
Author(s):  
Eltayib H. Ahmed Abakur ◽  
Tarig M.S. Alnour ◽  
Faisel Abuduhier ◽  
Fahad M.A. Albalawi ◽  
Khalid A.S. Alfifi
Keyword(s):  
Saudi Arabia ◽  
Mycobacterium Tuberculosis ◽  
Clinical Specimen ◽  
Rpob Gene ◽  
Rifampicin Resistance ◽  
Resistant Bacteria ◽  
Kingdom Of Saudi Arabia ◽  
Preliminary Stage ◽  
The North ◽  
Genotype Mtbdrplus

Purpose: Heteroresistant Mycobacterium tuberculosis (MTB) is defined as a group of drug-susceptible and resistant bacteria in a single clinical specimen from tuberculosis (TB) patients. Heteroresistance of MTB is considered a preliminary stage to full resistance. The present study aimed to determine the heteroresistance in Mycobacterium tuberculosis in Tabuk province, in the north of the Kingdom of Saudi Arabia. Method: GenoType MTBDRplus assay was used to determine mutations associated with isoniazid and rifampicin resistance. Results: A total number of 46 confirmed M. tuberculosis positive sputum samples were scanned for heteroresistance. The present study revealed 3 (6.5%) heteroresistant mutations to either rpoB gene alone, 2 (4.4%) to rpoB and 1 (2.2%) to inhA genes. Conclusion: The detection of heteroresistant mutations could guide the initiation of an appropriate regimen of treatment.

scholarly journals Biological microchips for detection of multiple drug resistant M. tuberculosis strains isolated in Kyrgyz Republic

2008 ◽  
pp. 64-66
Author(s):  
J. T. Isakova ◽  
Z. K. Goncharova ◽  
A. A. Aldashev
Keyword(s):  
Drug Resistance ◽  
Pulmonary Tuberculosis ◽  
Gene Mutations ◽  
Rpob Gene ◽  
Multiple Drug ◽  
Drug Resistant ◽  
Kyrgyz Republic ◽  
Newly Diagnosed ◽  
Single Drug ◽  
Multiple Drug Resistant

The aim of the study was to estimate spread of primary and secondary multiple drug resistant Mycobacterium tuberculosis (MBT) and to characterize rpoB, katG, inhA, and ahpC gene mutations of rifampicin (RIF) and isoniazid (INH) resistant MBT strains isolated from tuberculosis patients in Kyrgyz. We obtained 493 specimens from patients with pulmonary tuberculosis which were diagnosed based on clinical, X-ray, and bacteriological examination. Among them, newly diagnosed pulmonary tuberculosis was in 445 patients (90.2 %), and 48 of the patients (9.8 %) have already been treated for tuberculosis. Mutations of rpoB, KatG, inhA, and ahpC genes associated with RIF and INH resistance were detected by biological chip test. Sensitive MBT strains were detected in 47 % and resistant strains were in 53 % of the newly diagnosed patients. Single-drug resistance to RIF only was detected in 3 % of cases; resistance to INH was found in 20 %, resistance to both the drugs was detected in 30 % of the patients. In pre-treated patients single-drug resistance to RIF was defined in 4 % of cases, resistance to INH was in 8 %, resistance to both the drugs was estimated in 75 % of the patients. Therefore, we suppose that there is a high prevalence of multi-drug resistant MBT in Kyrgyz Republic: 30 % among newly diagnosed patients and 75 % among pre-treated patients. The main cause of RIF-resistance of MBT is Ser531→Leu mutation of rpoB gene, and the main cause of INHresistance is Ser315→Thr mutation of katG gene.

scholarly journals Comparison of neurocognitive changes among newly diagnosed tuberculosis patients with and without dysglycaemia

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Ernest Yorke ◽  
Vincent Boima ◽  
Ida Dzifa Dey ◽  
Vincent Ganu ◽  
Norah Nkornu ◽  
...  
Keyword(s):  
Newly Diagnosed ◽  
Tuberculosis Patients

scholarly journals Oxidative stress biomarkers in pulmonary tuberculosis patients in Gombe, North-eastern Nigeria

2019 ◽  
Vol 10 (6) ◽  
pp. 57-62 ◽  
Author(s):  
Mohammed Haruna Yeldu ◽  
Yakubu Ibrahim ◽  
Shehu Abubakar Akuyam ◽  
Isah Muhammad Danasabe ◽  
Buhari Shehu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pulmonary Tuberculosis ◽  
Total Antioxidant Status ◽  
Serum Levels ◽  
Newly Diagnosed ◽  
Oxidative Stress Biomarkers ◽  
Tuberculosis Patients ◽  
Stress Biomarkers ◽  
North Eastern ◽  
Total Antioxidant

Background: Oxidative stress may play an important role in the pathogenesis of pulmonary tuberculosis (PTB). To our knowledge there is paucity of data on the status of oxidative stress biomarkers among PTB patients in Gombe, North-eastern Nigeria. Our study was designed to evaluate the oxidative stress biomarkers in pulmonary tuberculosis patients in Gombe, North-eastern Nigeria. Aims and Objectives: To determine the serum levels of oxidative stress biomarkers among patients with pulmonary tuberculosis in Gombe metropolis, North-eastern Nigeria and to assess the correlation between the oxidative stress biomarkers in pulmonary tuberculosis patients. Materials and Methods: A cross sectional comparative study was conducted in a tertiary health care facility with 40 pulmonary tuberculosis (PTB) patients on anti-TB drugs treatment (ATT), 40 newly diagnosed PTB patients not yet on anti-TB drugs treatment (ATT-naïve) and 40 age- and sex-marched apparently healthy subjects (controls). Serum total antioxidant status (TAS), total oxidant status (TOS), malondialdehyde (MDA), nitric oxide (NO) and oxidative stress index (OSI) determined using standard techniques. Data was analysed using INSTAT® (Graph Pad Software Inc., La Jolla, CA, USA). Results: Serum levels of TOS, OSI, MDA and NO were significantly (p ˂ 0.001) increased in PTB patients (ATT and ATT-naïve) when compared with healthy individuals. Serum TAS and body mass index (BMI) were significantly (p ˂ 0.001) decreased in PTB patients when compared with healthy individuals. Serum TOS significantly correlated with serum OSI, MDA and NO in ATT-naïve PTB patients. Conclusion: This study observed an increased oxidative stress biomarkers and decreased total antioxidant status in newly diagnosed pulmonary tuberculosis patients and those on treatment. Our findings suggest that antioxidants supplementation and improved nutrition in the management of pulmonary tuberculosis patients may go a long way in preventing the oxidative onslaught and further complications in PTB patients.

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