scholarly journals Oxidative stress biomarkers in pulmonary tuberculosis patients in Gombe, North-eastern Nigeria

2019 ◽  
Vol 10 (6) ◽  
pp. 57-62 ◽  
Author(s):  
Mohammed Haruna Yeldu ◽  
Yakubu Ibrahim ◽  
Shehu Abubakar Akuyam ◽  
Isah Muhammad Danasabe ◽  
Buhari Shehu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pulmonary Tuberculosis ◽  
Total Antioxidant Status ◽  
Serum Levels ◽  
Newly Diagnosed ◽  
Oxidative Stress Biomarkers ◽  
Tuberculosis Patients ◽  
Stress Biomarkers ◽  
North Eastern ◽  
Total Antioxidant

Background: Oxidative stress may play an important role in the pathogenesis of pulmonary tuberculosis (PTB). To our knowledge there is paucity of data on the status of oxidative stress biomarkers among PTB patients in Gombe, North-eastern Nigeria. Our study was designed to evaluate the oxidative stress biomarkers in pulmonary tuberculosis patients in Gombe, North-eastern Nigeria. Aims and Objectives: To determine the serum levels of oxidative stress biomarkers among patients with pulmonary tuberculosis in Gombe metropolis, North-eastern Nigeria and to assess the correlation between the oxidative stress biomarkers in pulmonary tuberculosis patients. Materials and Methods: A cross sectional comparative study was conducted in a tertiary health care facility with 40 pulmonary tuberculosis (PTB) patients on anti-TB drugs treatment (ATT), 40 newly diagnosed PTB patients not yet on anti-TB drugs treatment (ATT-naïve) and 40 age- and sex-marched apparently healthy subjects (controls). Serum total antioxidant status (TAS), total oxidant status (TOS), malondialdehyde (MDA), nitric oxide (NO) and oxidative stress index (OSI) determined using standard techniques. Data was analysed using INSTAT® (Graph Pad Software Inc., La Jolla, CA, USA). Results: Serum levels of TOS, OSI, MDA and NO were significantly (p ˂ 0.001) increased in PTB patients (ATT and ATT-naïve) when compared with healthy individuals. Serum TAS and body mass index (BMI) were significantly (p ˂ 0.001) decreased in PTB patients when compared with healthy individuals. Serum TOS significantly correlated with serum OSI, MDA and NO in ATT-naïve PTB patients. Conclusion: This study observed an increased oxidative stress biomarkers and decreased total antioxidant status in newly diagnosed pulmonary tuberculosis patients and those on treatment. Our findings suggest that antioxidants supplementation and improved nutrition in the management of pulmonary tuberculosis patients may go a long way in preventing the oxidative onslaught and further complications in PTB patients.

Effects of chromium supplementation on oxidative Stress biomarkers

2021 ◽  
pp. 1-11
Author(s):  
Mohammad Reza Amini ◽  
Fatemeh Sheikhhossein ◽  
Farhang Djafari ◽  
Alireza Jafari ◽  
Kurosh Djafarian ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Randomized Clinical Trials ◽  
Meta Analysis ◽  
Total Antioxidant Status ◽  
Thiobarbituric Acid ◽  
Protein Carbonyl ◽  
Chromium Picolinate ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Total Antioxidant

Abstract. Aim: This systematic review and meta-analysis aimed to evaluate the effects of chromium supplementation on oxidative stress biomarkers such as superoxide dismutase (SOD), glutathione (GSH), glutathione peroxidase (GPX), malondialdehyde (MDA), total antioxidant status (TAS), thiobarbituric acid reactive substances (TBARS), catalase (CAT), nitric oxide (NO), total antioxidant capacity (TAC) and protein carbonyl. Methods: Relevant studies, published from inception until July 2019, were searched through PubMed/Medline, Scopus, ISI Web of Science, Embase, and Google Scholar. All randomized clinical trials investigating the effect of chromium supplementation on oxidative stress were included. Results: Out of 252 citations, 10 trials that enrolled 595 subjects were included. Chromium supplementation resulted in a significant increase in GSH (WMD: 64.79 mg/dl, 95% CI: 22.43 to 107.15; P=0.003) but no significant change in MDA, TAS, TBARS levels, SOD, CAT levels and GPX. Chromium picolinate supplementation resulted in a significant increase in TAC while failing to have a significant effect on NO. Moreover, both chromium picolinate and chromium dinicocysteinate supplementation reduced protein carbonyl levels. Conclusion: Overall, this meta-analysis demonstrated that chromium supplementation increased GSH without any significant changes in the mean of GPX, MDA, TAS, TBARS, CAT and SOD.

scholarly journals Glycemic control and its impact on oxidative stress biomarkers in type 2 diabetic patients treated with metformin: a cross-sectional analysis

2019 ◽  
Vol 29 (2) ◽  
pp. 33630
Author(s):  
Ismaila A. Lasisi ◽  
Kamoru A. Adedokun ◽  
Musiliu A. Oyenike ◽  
Musa A. Muhibi ◽  
Ramat T. Kamorudeen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Glycemic Control ◽  
Hemoglobin A1c ◽  
Diabetic Patients ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Fasting Glycemia ◽  
Chronic Hyperglycemia ◽  
Glycemia Level ◽  
Total Antioxidant

AIMS: Evidence shows that diabetic patients may be predisposed to oxidative stress owing to increased glyco-oxidation and lipid peroxidation processes in consequence of chronic hyperglycemia. However, there is dearth of information whether glycemic control positively affects the antioxidant defense system in type 2 diabetes mellitus (T2DM). We investigated the potential association between glycemic control and oxidative stress biomarkers in controlled and uncontrolled diabetic states. METHODS: After obtaining ethical clearance, we included patients receiving metformin with glycated hemoglobin A1c ˂7.0% (glycemic control); newly diagnosed T2DM patients without glycemic control with hemoglobin A1c ˃7.0%; and apparently healthy normoglycemic individuals. The following biomarkers were determined: fasting glycemia level, malondialdehyde, glutathione peroxidase activity, catalase activity, total antioxidant capacity and total cholesterol level. The comparisons between the groups were made by ANOVA. RESULTS: The participants were 260 in number: 80 with controlled diabetes, 80 uncontrolled and 100 controls. All participants were between 40 and 71 years old. Fasting glycemia level and hemoglobin A1c showed significant reductions (p<0.05) in controlled T2DM against the uncontrolled T2DM group, all the same both were significantly higher (p<0.05) against the controls. Likewise, malondialdehyde levels showed significant elevations (p<0.05) correspondingly in both uncontrolled and controlled T2DM against the controls, accompanied with significant reductions (p<0.05) in the antioxidative enzyme activities (glutathione peroxidase activity and catalase activity) and total antioxidant capacity levels against the controls. In addition, total cholesterol was significantly reduced (p<0.05) in controlled T2DM against both uncontrolled T2DM and controls, respectively. There were significant correlations between hemoglobin A1c and oxidative stress biomarkers (p<0.05). CONCLUSION: There was no remarkable difference in oxidative stress states between glycemic controlled and uncontrolled T2DM, despite differences in their fasting glycemia and glycated hemoglobin levels. Our data, therefore, suggest that chronic hyperglycemia and possibly anti-diabetic medicationmay both equally associate with oxidative stress. 

scholarly journals Antioxidant Effects of a Hydroxytyrosol-Based Pharmaceutical Formulation on Body Composition, Metabolic State, and Gene Expression: A Randomized Double-Blinded, Placebo-Controlled Crossover Trial

2017 ◽  
Vol 2017 ◽  
pp. 1-14 ◽  
Author(s):  
Carmela Colica ◽  
Laura Di Renzo ◽  
Domenico Trombetta ◽  
Antonella Smeriglio ◽  
Sergio Bernardini ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Body Composition ◽  
Crossover Trial ◽  
Total Antioxidant Status ◽  
Thiol Group ◽  
Oxidative Stress Biomarkers ◽  
Total Antioxidant ◽  
Stress Related Genes ◽  
Double Blinded

Hydroxytyrosol (HT) plays a significant role in cardiovascular disease (CVD) protection, and its metabolites are able to protect from the endothelial dysfunction commonly present in atherosclerosis. This randomized double-blinded, placebo-controlled crossover trial determined the effect in healthy volunteers of two gastroresistant capsules containing 15 mg/day of HT, for a 3-week period (HTT). Evaluation of nutritional status, serum metabolites, oxidative stress biomarkers, and gene expression of 9 genes related to oxidative stress, inflammation, and CVDs was performed. Oxidation biomarkers like thiol group (p=0.001), total antioxidant status (TAS) (p=0.001), superoxide dismutase 1 (SOD1) (2−ΔΔCt = 3.7), and plasma concentration of HT (2.83μg·mL−1) were significantly increased, while nitrite (p=0.001), nitrate (p=0.001), and malondialdehyde (MDA) (p=0.02) were drastically reduced after HTT. A significant reduction of body fat mass percentage (p=0.01), suprailiac skinfold (p=0.01), and weight (p=0.04; Δ% = −0.46%) was observed after HTT. This study shows that regular intake of 15 mg/day of HT changed body composition parameters and modulated the antioxidant profile and the expression of inflammation and oxidative stress-related genes. However, it is advisable to personalize HT doses in order to exert its health benefits in CVD prevention and protection of LDL-C particles from oxidative damage. This trial is registered with ClinicalTrials.govNCT01890070.

scholarly journals Changes of Inflammatory and Oxidative Stress Biomarkers in Dogs with Different Stages of Heart Failure

2020 ◽  
Author(s):  
Camila Peres RUBIO ◽  
Ahmet SARIL ◽  
Meriç KOCATURK ◽  
Ryou TANAKA ◽  
Jorgen KOCH ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heart Failure ◽  
Acute Phase Proteins ◽  
Staging System ◽  
Serum Levels ◽  
Left Ventricular ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Anti Oxidant ◽  
And Oxidative Stress

Abstract Background: Heart failure (HF) has been associated with changes in inflammatory and oxidative-stress biomarkers. This study aimed to evaluate the changes of a panel of inflammatory and oxidative stress biomarker in dogs with different stages of HF and its relation with the severity of the disease and the echocardiographic changes. Dogs with HF as a result of myxomatous mitral valve degeneration or dilated cardiomyopathy were included. A total of 29 dogs were classified as stage-A (healthy), B (asymptomatic dogs), C (symptomatic dogs) and D (dogs with end-stage-HF) according to the ACVIM-staging-system. Serum cytokines, and inflammatory and oxidative stress markers were evaluated.Results: KC-like as an inflammatory cytokine was significantly increased in dogs of stage-C (P < 0.01) and -D (P < 0.05) compared with stage-A and -B. Stage-D Dogs showed significantly higher serum CRP and Hp (P < 0.05) but lower serum anti-oxidant capacity (PON1, TEAC, CUPRAC, and thiol) compared to stage-A and -B (P < 0.05). After the treatment, serum levels of CRP, Hp and KC-like decreased but serum anti-oxidant levels increased compared to their pre-treatment values. Left ventricular dimension and LA/Ao ratio correlated positively with CRP, MCP-1, and KC-like but negatively with PON1, GM-CSF, IL-7 and antioxidant biomarkers (P < 0.01).Conclusion: our results indicated that dogs with advanced-stages of HF show increases in positive acute-phase proteins and selected inflammatory cytokines (KC-like), and decreases in antioxidant biomarkers, indicating that inflammation and oxidative-stress act as collaborative partners in the pathogenesis of HF. KC-like may be a more useful parameter to monitor disease progression and treatment efficacy of dogs with severe-HF.

Determination of Cytokines and Oxidative Stress Biomarkers in Cognitive Impairment Induced by Methylmalonic Acidemia

2021 ◽  
Vol 28 (3) ◽  
pp. 178-186
Author(s):  
Qiliang Li ◽  
Hong Jin ◽  
Ying Liu ◽  
Yu Rong ◽  
Tana Yang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cognitive Impairment ◽  
Methylmalonic Acid ◽  
Serum Levels ◽  
High Concentration ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Tnf Α ◽  
Iq Scores ◽  
And Oxidative Stress

<b><i>Objective:</i></b> Methylmalonic acidemia (MMA) is the most common organic acidemia in children. Many patients with MMA suffered from cognitive impairments. The aim of this study was to identify the significance of cytokines and oxidative stress biomarkers in MMA-induced cognitive impairment. <b><i>Methods:</i></b> We enrolled 64 children with combined MMA and homocystinuria and 64 age- and sex-matched healthy volunteers. Participants were subsequently classified as with or without cognitive impairments using a uniform neuropsychological assessment test. Serum samples were collected. The serum levels of cytokines and oxidative stress biomarkers were measured using the ELISA or chemical methods. <b><i>Results:</i></b> Compared to control group, the serum levels of tumor necrosis factor (TNF)-α, interleukin (IL)-6, malondialdehyde (MDA), and nitric oxide (NO) in the MMA patients increased markedly (<i>p</i> &#x3c; 0.05); glutathione (GSH) and superoxide dismutase (SOD) decreased obviously (<i>p</i> &#x3c; 0.01). The levels of IL-6, TNF-α, NO, and MDA in the serum were negatively associated with DQ or IQ scores. The levels of GSH and SOD in the serum were positively correlated with DQ or IQ scores. After receiver operating characteristic curve analysis, NO was the most useful individual marker for distinguishing the cognitive dysfunction, corresponding to the area under ROC curve (AUC) of 0.82 (95% CI, 0.74–0.91), sensitivity of 76.60%, and specificity of 80.25%. GSH and MDA were also useful for diagnosis of MMA-induced cognitive dysfunction, corresponding to the AUC of 0.80 (95% CI, 0.70–0.89), and 0.73 (95% CI, 0.63–0.82), respectively. The sensitivity and specificity of GSH were 72.34 and 80.25%, respectively. The sensitivity and specificity of MDA were 85.11 and 51.85%, respectively. <b><i>Conclusions:</i></b> The high-concentration methylmalonic acid in the blood induced immune cells to release pro-inflammatory cytokines such as TNF-α and IL-6. These cytokines and high-concentration methylmalonic acid stimulated the immune cells to produce reactive oxygen species (ROS) and reactive nitrogen species (RNS). The serum methylmalonic acid, cytokines, ROS, and RNS were across the blood-brain barrier and induced cognitive impairment. The small molecule substances such as serum NO, MDA, and GSH participated in the process of neuroinflammation and oxidative stress injury induced by MMA and could be useful for distinguishing the cognitive impairment.

scholarly journals Attenuation of Oxidative Stress and Inflammation by Portulaca oleracea in Streptozotocin-Induced Diabetic Rats

2017 ◽  
Vol 22 (4) ◽  
pp. 562-566 ◽  
Author(s):  
Saeed Samarghandian ◽  
Abasalt Borji ◽  
Tahereh Farkhondeh
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Portulaca Oleracea ◽  
Tnf Α ◽  
Total Antioxidant ◽  
Factor Α ◽  
Necrosis Factor

The present study was designed to investigate the protective effect of the aqueous extract of Portulaca oleracea against hyperglycemic, oxidative damage and inflammation in the serum of streptozotocin (STZ)-induced diabetic rats. In the present study, the rats were divided into the following groups of 8 animals each: control, untreated diabetic, 3 Portulaca oleracea (100, 200, 400 mg/kg/d)–treated diabetic groups. At the end of the 4-week period, glucose, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), malondialdehyde (MDA), glutathione (GSH), and total antioxidant status (TAS) levels were measured. STZ caused an elevation in the serum levels of glucose, MDA, IL-6, and TNF-α with reduction in the levels of GSH and TAS ( P < .01). Portulaca oleracea ameliorated glucose, MDA, IL-6, TNF-α, GSH, and TAS levels in diabetic groups versus to the untreated groups ( P < .05). Taken together, Portulaca oleracea prevented hyperglycemia by preventing the oxidative stress and inflammation.

scholarly journals Serum Levels of Stress Hormones and Oxidative Stress Biomarkers Differ according to Sasang Constitutional Type

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Hyeong Geug Kim ◽  
Yoon Jung Kim ◽  
Yo Chan Ahn ◽  
Chang Gue Son
Keyword(s):  
Oxidative Stress ◽  
Stress Hormones ◽  
Serum Cortisol ◽  
Serum Levels ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Constitutional Type ◽  
Sasang Constitutional Types ◽  
And Oxidative Stress ◽  
Better Than

Objectives.This study investigated whether Sasang constitutional type is associated with differences in the serum levels of stress hormones and oxidative stress.Methods.A total of 236 participants (77 males and 159 females) were enrolled. The serum levels of cortisol, adrenaline, reactive oxygen species (ROS), and malondialdehyde (MDA) were analyzed.Results.The distribution of Sasang constitutional types was as follows: Taeumin, 35.6%; Soumin, 33.0%; and Soyangin, 31.4%. The serum cortisol levels of Taeumin were significantly lower than Soumin (p<0.1in both sexes) and Soyangin (p<0.05in males andp<0.1in females). The adrenaline levels were also significantly lower in Taeumin than in Soumin (p<0.05in males andp<0.1in females) and Soyangin (p<0.1in males). Serum ROS levels were significantly higher in Soyangin than in Taeumin and Soumin (p<0.05in males), whereas MDA levels were significantly lower in Taeumin compared with Soumin and Soyangin (p<0.05in males andp<0.1in females).Conclusion.Taeumin type may tolerate psychological or oxidative stress better than other types, which suggests a biological mechanism to explain the different pathophysiological features of Sasang constitutional types.

scholarly journals Change in Oxidative Stress Biomarkers During 30 Days in Saturation Dive: A Pilot Study

2020 ◽  
Vol 17 (19) ◽  
pp. 7118
Author(s):  
Simona Mrakic-Sposta ◽  
Alessandra Vezzoli ◽  
Federica D’Alessandro ◽  
Matteo Paganini ◽  
Cinzia Dellanoce ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Decompression Sickness ◽  
Redox Status ◽  
Ros Production ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Total Antioxidant ◽  
Level Elevation ◽  
Nitric Oxide Metabolites ◽  
Stress Damage

Saturation diving allows divers to reduce the risk of decompression sickness while working at depth for prolonged periods but may increase reactive oxygen species (ROS) production. Such modifications can affect endothelial function by exacerbating oxidative stress. This study investigated the effects of saturation diving on oxidative stress damage. Redox status was evaluated through: ROS production; total antioxidant capacity (TAC); nitric oxide metabolites (NOx); nitrotyrosine (3-NT); and lipid peroxidation (8-iso-PGF2α) assessment. Creatinine and neopterin were analyzed as markers of renal function and damage. Measurements were performed on saliva and urine samples obtained at four time points: pre; deep; post; and 24 h post. Four divers were included in the study. After the saturation dive (post), significant (p < 0.05) increases in ROS (0.12 ± 0.03 vs. 0.36 ± 0.06 µmol.min−1), TAC (1.88 ± 0.03 vs. 2.01 ± 0.08 mM), NOx (207.0 ± 103.3 vs. 441.8 ± 97.3 µM), 3-NT (43.32 ± 18.03 vs. 18.64 ± 7.45 nM·L−1), and 8-iso-PGF2α (249.7 ± 45.1 vs. 371.9 ± 54.9 pg·mg−1 creatinine) were detected. Markers of renal damage were increased as well after the end of the saturation dive (creatinine 0.54 ± 0.22 vs. 2.72 ± 1.12 g-L−1; neopterin 73.3 ± 27.9 vs. 174.3 ± 20.53 μmol·mol−1 creatinine). These results could ameliorate commercial or military diving protocols or improve the understanding of symptoms caused by oxygen level elevation.

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