Dietary supplementation with copper nanoparticles influences the markers of oxidative stress and modulates vasodilation of thoracic arteries in young Wistar rats

The effects of N-acetylcarnosine (NAC)-contained eye drop ‘Clarastil’ on a model of adrenaline-induced high intraocular pressure (IOP) in Wistar rats were studied. The retina ultrastructure and markers of oxidative stress have been studied. NAC was found to have no significant effect on edema in the retinal ultrastructure, did not reduce endothelial thickening and histogemic barrier, and accordingly did not affect the value of IOP after prolonged adrenaline administration. However, the introduction of the eye drop prevented the swelling of the mitochondria, the formation of vacuolated crystals and probably stimulated energy production as a compensatory mechanism under conditions of hypercatecholemia. In addition, NAC significantly reduced adrenaline-induced overproduction of reactive oxygen species and lipid peroxidation products in eye tissues, indicating its antioxidant effect.

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Study of Dynamics of Immunobiochemical Parameters and Pharmacological Interferences in the Metabolic Syndrome

Revista de Chimie ◽

10.37358/rc.18.6.6353 ◽

2018 ◽

Vol 69 (6) ◽

pp. 1493-1497

Author(s):

Ana Roxana Ganceanu Rusu ◽

Mititelu Liliana Tartau ◽

Cristian Statescu ◽

Mihaela Boanca ◽

Vladimir Poroch ◽

...

Keyword(s):

Oxidative Stress ◽

Metabolic Syndrome ◽

Wistar Rats ◽

Serum Cortisol ◽

Converting Enzyme ◽

Cholesterol Diet ◽

The Metabolic Syndrome ◽

Minute Interval ◽

Markers Of Oxidative Stress ◽

Inflammatory Drugs

The metabolic syndrome (MetS) represents a frequent disorder of the present age, with increased global prevalence, with complex etiopathogenesis and physiopathology, with great impact upon the patients, society and economy and which often is difficult to treat. The main aim of the study was the evaluation of the pharmacodynamic effects of associated angiotensin-converting enzyme (ACE) inhibitors with non-steroidal anti-inflammatory drugs (NSAIDs) on blood pressure values and markers of oxidative stress in rats with MetS. Material and methods: 9 groups of 6 Wistar rats weighing between 150-200g with uniform gender distribution were subjected to cholesterol diet. During 4 weeks, their exercise capacities were analyzed over a 10-minute interval after administration of the test substances. Results: All groups who received cholesterol showed significant increases in serum cortisol. The group receiving Enalapril noted the highest levels of superoxide dismutase (SOD). Conclusions: Comparing the results obtained in this study with the literature, it was noted that the administration of ACE and / or NSAIDs significantly improves the process of chronic inflammation.

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Extract of the plant Cotinus coggygria Scop. attenuates pyrogallol-induced hepatic oxidative stress in Wistar rats

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y11-043 ◽

2011 ◽

Vol 89 (6) ◽

pp. 401-411 ◽

Cited By ~ 8

Author(s):

Sanja Matić ◽

Snežana Stanić ◽

Desanka Bogojević ◽

Melita Vidaković ◽

Nevena Grdović ◽

...

Keyword(s):

Oxidative Stress ◽

Acute Phase ◽

Wistar Rats ◽

Thiobarbituric Acid ◽

Hepatoprotective Activity ◽

Acute Phase Reactants ◽

Markers Of Oxidative Stress ◽

Cotinus Coggygria

To examine the protective potential of the Cotinus coggygria Scop. methanol extract, Wistar rats were treated with the hepatotoxic compound pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The ability of the extract to counteract the oxidative stress was examined in rats that were injected with the extract intraperitoneally (500 mg·(kg body weight)–1) either 2 or 12 h before the pyrogallol treatment. The extract possesses a reducing activity in vitro and an ability to chelate the ferrous ion both in vivo and in vitro. Application of the extract prior to pyrogallol treatment led to a decrease in the levels of thiobarbituric acid-reactive substances, aspartate aminotransferase, and alanine aminotransferase, increased activities of antioxidant enzymes and attenuation of DNA damage, as well as increased Akt activity and inhibition of NF-κB protein expression. Treatment with the extract 12 h prior to pyrogallol administration was more effective in suppressing pyrogallol-induced oxidative damage than the 2 h pretreatment. Extract administration promoted an increase in acute phase reactants haptoglobin and α2-macroglobulin that was short of a full-fledged acute phase response. Administration of the extract considerably improved the markers of oxidative stress, thus revealing a potential hepatoprotective activity. Our results suggest that Akt activation, NF-κB inhibition, and induction of the acute phase play important roles in mediating hepatic protection by the extract. The greater effectiveness of the 12 h pretreatment with extract points to the important role that preconditioning assumes in improving resistance to subsequent exposure to oxidative stress.

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Peripheral Oxidation Markers in Down Syndrome Patients: The Better and the Worse

Disease Markers ◽

10.1155/2021/5581139 ◽

2021 ◽

Vol 2021 ◽

pp. 1-9

Author(s):

Dominik Szwajgier ◽

Ewa Baranowska-Wójcik ◽

Joanna Grzelczyk ◽

Wioletta Żukiewicz-Sobczak

Keyword(s):

Oxidative Stress ◽

Down Syndrome ◽

Matched Control ◽

Dietary Supplementation ◽

Dose Effect ◽

Control Groups ◽

Markers Of Oxidative Stress ◽

Nutritional Studies ◽

Peripheral Markers ◽

Healthy Persons

Oxidative stress plays an important role in Down syndrome (DS) pathology since the gene dose effect leads to abnormal levels of certain enzymes and metabolites. In this review, we focused on relatively easy-to-obtain, peripheral markers of oxidative stress and inflammation, in order to compare the levels of these markers in DS patients and chromosomally healthy persons. Studies taking into account age- and sex-matched control groups were of particular interest in this context. We analyzed the factors that influence the levels of said markers in both groups (i.e., the usefulness of the markers), including the age of DS patients, occurrence of regular trisomy 21 or mosaicism, physical activity of patients, and the onset of Alzheimer’s disease in DS. This paper was conceived as a handbook—to help for selecting suitable, easy-to-obtain markers for monitoring of the health status of DS patients (e.g., in nutritional studies and during dietary supplementation).

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Anise Hyssop Agastache foeniculum Increases Lifespan, Stress Resistance, and Metabolism by Affecting Free Radical Processes in Drosophila

Frontiers in Physiology ◽

10.3389/fphys.2020.596729 ◽

2020 ◽

Vol 11 ◽

Author(s):

Olha M. Strilbytska ◽

Alina Zayachkivska ◽

Alexander Koliada ◽

Fabio Galeotti ◽

Nicola Volpi ◽

...

Keyword(s):

Oxidative Stress ◽

Free Radical ◽

Antioxidant Properties ◽

Dietary Supplementation ◽

Egg Laying ◽

Independent Manner ◽

Metabolic Traits ◽

Elevated Protein ◽

Markers Of Oxidative Stress ◽

Leaf Powder

Anise hyssop, Agastache foeniculum, is a widely used medicinal herb with known antioxidant properties. We studied how dietary supplementation with dried A. foeniculum leaf powder affected physiological and metabolic traits as well as activities of antioxidant enzymes and markers of oxidative stress in Drosophila melanogaster. Dietary hyssop extended the lifespan in a sex and genotype independent manner over a broad range of concentrations up to 30 mg/ml. Dietary supplementation with the herb significantly increased fecundity, resistance to oxidative stress and starvation. Higher transcript levels of Drosophila insulin-like peptide (dilp2) and decreased dilp3 and dilp6 transcripts together with increased levels of glycogen and triacylglycerols support an alteration of insulin signaling by the plant extract. Increased enzymatic activities of superoxide dismutase and aconitase as well as elevated protein and low molecular mass thiols also supported an alteration of free radical process in flies treated with dietary A. foeniculum leaf powder. Thus, physiological and metabolic traits as well as free radical processed may be affected by active compounds detected in extracts of anise hyssop leaves and contribute to the increased lifespan and reproductive (egg-laying) activity observed.

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Effect of Pomegranate (Punica granatum L.) Seed Oil on Markers of Oxidative Stress Induced by Carbon Tetrachloride in Wistar Rats

International Journal of Food Science Nutrition and Dietetics ◽

10.19070/2326-3350-si05001 ◽

2015 ◽

pp. 1-8

Author(s):

Melo ILP ◽

◽

Silva AMO ◽

Carvalho EBT ◽

Yoshime LT ◽

...

Keyword(s):

Oxidative Stress ◽

Carbon Tetrachloride ◽

Wistar Rats ◽

Seed Oil ◽

Punica Granatum ◽

Markers Of Oxidative Stress ◽

Punica Granatum L

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Behavioural and biochemical effects of one-week exposure to aflatoxin B1 and aspartame in male Wistar rats

World Mycotoxin Journal ◽

10.3920/wmj2018.2424 ◽

2019 ◽

Vol 12 (3) ◽

pp. 293-305

Author(s):

N.S. Souto ◽

M. Dassi ◽

A.C.M. Braga ◽

E.V.F. Rosa ◽

M.R. Fighera ◽

...

Keyword(s):

Oxidative Stress ◽

Wistar Rats ◽

Artificial Sweetener ◽

Glutathione S Transferase ◽

Obsessive Compulsive ◽

Male Wistar Rats ◽

Markers Of Oxidative Stress ◽

Liver And Kidney ◽

Aflatoxin B ◽

Gst Activity

Food products are susceptible to contamination by mycotoxins, and aflatoxin B1 (AFB1) stands as the most toxic among them. AFB1 intoxication results in distinct signs, including widespread systemic toxicity. Aspartame (ASP) is an artificial sweetener used as a sugar substitute in many products, and compelling evidence indicates ASP can be toxic. Interestingly, mechanisms underlying ASP and AFB1 toxicity involve oxidative stress. In this context, concomitant use of ASP and AFB1 in a meal may predispose to currently unidentified behavioural and biochemical changes. Therefore, we evaluated the effect of AFB1 (250 μg/kg, intragastrically (i.g.)) and/or ASP (75 mg/kg, i.g.) exposure for 7 days on behavioural and biochemical markers of oxidative stress in male Wistar rats. AFB1 and/or ASP increased hepatic glutathione S-transferase (GST) activity when compared to controls. In the kidneys, increased GST activity was detected in AFB1 and AFB1+ASP groups. In addition, AFB1 and or ASP elicited behavioural changes in the open field, marble burying and splash tests, however no additive effects were detected. Altogether, present data suggest AFB1 and ASP predispose to anxiety- and obsessive-compulsive-like symptoms, as well as to enzymatic defence system imbalance in liver and kidney of Wistar rats.

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