scholarly journals Transcripts of wheat at a target locus on chromosome 6B associated with increased yield, leaf mass and chlorophyll index under combined drought and heat stress

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0241966
Author(s):  
Jessica Schmidt ◽  
Melissa Garcia ◽  
Chris Brien ◽  
Priyanka Kalambettu ◽  
Trevor Garnett ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Tolerance ◽  
Candidate Genes ◽  
Abiotic Stress Tolerance ◽  
Enrichment Analysis ◽  
Genotyping By Sequencing ◽  
Differentially Expressed ◽  
Pathway Enrichment Analysis ◽  
Heat Stress Tolerance ◽  
Drought And Heat Stress

Drought and heat stress constrain wheat (Triticum aestivum L.) yields globally. To identify putative mechanisms and candidate genes associated with combined drought and heat stress tolerance, we developed bread wheat near-isogenic lines (NILs) targeting a quantitative trait locus (QTL) on chromosome 6B which was previously associated with combined drought and heat stress tolerance in a diverse panel of wheats. Genotyping-by-sequencing was used to identify additional regions that segregated in allelic pairs between the recurrent and the introduced exotic parent, genome-wide. NILs were phenotyped in a gravimetric platform with precision irrigation and exposed to either drought or to combined drought and heat stress from three days after anthesis. An increase in grain weight in NILs carrying the exotic allele at 6B locus was associated with thicker, greener leaves, higher photosynthetic capacity and increased water use index after re-watering. RNA sequencing of developing grains at early and later stages of treatment revealed 75 genes that were differentially expressed between NILs across both treatments and timepoints. Differentially expressed genes coincided with the targeted QTL on chromosome 6B and regions of genetic segregation on chromosomes 1B and 7A. Pathway enrichment analysis showed the involvement of these genes in cell and gene regulation, metabolism of amino acids and transport of carbohydrates. The majority of these genes have not been characterized previously under drought or heat stress and they might serve as candidate genes for improved abiotic stress tolerance.

scholarly journals Humic acid enhances heat stress tolerance via transcriptional activation of Heat-Shock Proteins in Arabidopsis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Joon-Yung Cha ◽  
Sang-Ho Kang ◽  
Imdad Ali ◽  
Sang Cheol Lee ◽  
Myung Geun Ji ◽  
...  
Keyword(s):  
Heat Stress ◽  
Humic Acid ◽  
Heat Shock ◽  
Stress Tolerance ◽  
Transcriptional Activation ◽  
Molecular Mechanisms ◽  
Agronomic Traits ◽  
Enrichment Analysis ◽  
Gene Families ◽  
Heat Stress Tolerance

Abstract Humic acid (HA) is composed of a complex supramolecular association and is produced by humification of organic matters in soil environments. HA not only improves soil fertility, but also stimulates plant growth. Although numerous bioactivities of HA have been reported, the molecular evidences have not yet been elucidated. Here, we performed transcriptomic analysis to identify the HA-prompted molecular mechanisms in Arabidopsis. Gene ontology enrichment analysis revealed that HA up-regulates diverse genes involved in the response to stress, especially to heat. Heat stress causes dramatic induction in unique gene families such as Heat-Shock Protein (HSP) coding genes including HSP101, HSP81.1, HSP26.5, HSP23.6, and HSP17.6A. HSPs mainly function as molecular chaperones to protect against thermal denaturation of substrates and facilitate refolding of denatured substrates. Interestingly, wild-type plants grown in HA were heat-tolerant compared to those grown in the absence of HA, whereas Arabidopsis HSP101 null mutant (hot1) was insensitive to HA. We also validated that HA accelerates the transcriptional expression of HSPs. Overall, these results suggest that HSP101 is a molecular target of HA promoting heat-stress tolerance in Arabidopsis. Our transcriptome information contributes to understanding the acquired genetic and agronomic traits by HA conferring tolerance to environmental stresses in plants.

scholarly journals Novel Alleles for Combined Drought and Heat Stress Tolerance in Wheat

2020 ◽  
Vol 10 ◽  
Author(s):  
Jessica Schmidt ◽  
Penny J. Tricker ◽  
Paul Eckermann ◽  
Priyanka Kalambettu ◽  
Melissa Garcia ◽  
...  
Keyword(s):  
Heat Stress ◽  
Stress Tolerance ◽  
Heat Stress Tolerance ◽  
Novel Alleles ◽  
Drought And Heat Stress

scholarly journals Drought and heat stress tolerance screening in wheat using computed tomography

Plant Methods ◽  
2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Jessica Schmidt ◽  
Joelle Claussen ◽  
Norbert Wörlein ◽  
Anja Eggert ◽  
Delphine Fleury ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Heat Stress ◽  
Stress Tolerance ◽  
Heat Stress Tolerance ◽  
Drought And Heat Stress

scholarly journals Enhanced drought and heat stress tolerance of tobacco plants with ectopically enhanced cytokinin oxidase/dehydrogenase gene expression

2013 ◽  
Vol 64 (10) ◽  
pp. 2805-2815 ◽  
Author(s):  
Hana Macková ◽  
Marie Hronková ◽  
Jana Dobrá ◽  
Veronika Turečková ◽  
Ondřej Novák ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heat Stress ◽  
Stress Tolerance ◽  
Cytokinin Oxidase ◽  
Tobacco Plants ◽  
Dehydrogenase Gene ◽  
Heat Stress Tolerance ◽  
Drought And Heat Stress

scholarly journals Major QTLs and Potential Candidate Genes for Heat Stress Tolerance Identified in Chickpea (Cicer arietinum L.)

2021 ◽  
Vol 12 ◽  
Author(s):  
Uday Chand Jha ◽  
Harsh Nayyar ◽  
Ramesh Palakurthi ◽  
Rintu Jha ◽  
Vinod Valluri ◽  
...  
Keyword(s):  
Heat Stress ◽  
Heat Shock ◽  
Stress Tolerance ◽  
Candidate Genes ◽  
Cicer Arietinum ◽  
Nitrogen Balance ◽  
Major Qtls ◽  
Heat Stress Tolerance ◽  
Heat Tolerant ◽  
Balance Index

In the context of climate change, heat stress during the reproductive stages of chickpea (Cicer arietinum L.) leads to significant yield losses. In order to identify the genomic regions responsible for heat stress tolerance, a recombinant inbred line population derived from DCP 92-3 (heat sensitive) and ICCV 92944 (heat tolerant) was genotyped using the genotyping-by-sequencing approach and evaluated for two consecutive years (2017 and 2018) under normal and late sown or heat stress environments. A high-density genetic map comprising 788 single-nucleotide polymorphism markers spanning 1,125 cM was constructed. Using composite interval mapping, a total of 77 QTLs (37 major and 40 minor) were identified for 12 of 13 traits. A genomic region on CaLG07 harbors quantitative trait loci (QTLs) explaining >30% phenotypic variation for days to pod initiation, 100 seed weight, and for nitrogen balance index explaining >10% PVE. In addition, we also reported for the first time major QTLs for proxy traits (physiological traits such as chlorophyll content, nitrogen balance index, normalized difference vegetative index, and cell membrane stability). Furthermore, 32 candidate genes in the QTL regions that encode the heat shock protein genes, heat shock transcription factors, are involved in flowering time regulation as well as pollen-specific genes. The major QTLs reported in this study, after validation, may be useful in molecular breeding for developing heat-tolerant superior lines or varieties.

scholarly journals Differential Morpho-Physiological and Transcriptomic Responses to Heat Stress in Two Blueberry Species

2021 ◽  
Vol 22 (5) ◽  
pp. 2481
Author(s):  
Jodi Callwood ◽  
Kalpalatha Melmaiee ◽  
Krishnanand P. Kulkarni ◽  
Amaranatha R. Vennapusa ◽  
Diarra Aicha ◽  
...  
Keyword(s):  
Heat Stress ◽  
Molecular Mechanisms ◽  
Stomatal Closure ◽  
Enrichment Analysis ◽  
Leaf Size ◽  
Vaccinium Corymbosum ◽  
Climatic Conditions ◽  
Differentially Expressed ◽  
Transcriptomic Changes ◽  
Go Terms

Blueberries (Vaccinium spp.) are highly vulnerable to changing climatic conditions, especially increasing temperatures. To gain insight into mechanisms underpinning the response to heat stress, two blueberry species were subjected to heat stress for 6 and 9 h at 45 °C, and leaf samples were used to study the morpho-physiological and transcriptomic changes. As compared with Vaccinium corymbosum, Vaccinium darrowii exhibited thermal stress adaptation features such as small leaf size, parallel leaf orientation, waxy leaf coating, increased stomatal surface area, and stomatal closure. RNAseq analysis yielded ~135 million reads and identified 8305 differentially expressed genes (DEGs) during heat stress against the control samples. In V. corymbosum, 2861 and 4565 genes were differentially expressed at 6 and 9 h of heat stress, whereas in V. darrowii, 2516 and 3072 DEGs were differentially expressed at 6 and 9 h, respectively. Among the pathways, the protein processing in the endoplasmic reticulum (ER) was the highly enriched pathway in both the species: however, certain metabolic, fatty acid, photosynthesis-related, peroxisomal, and circadian rhythm pathways were enriched differently among the species. KEGG enrichment analysis of the DEGs revealed important biosynthesis and metabolic pathways crucial in response to heat stress. The GO terms enriched in both the species under heat stress were similar, but more DEGs were enriched for GO terms in V. darrowii than the V. corymbosum. Together, these results elucidate the differential response of morpho-physiological and molecular mechanisms used by both the blueberry species under heat stress, and help in understanding the complex mechanisms involved in heat stress tolerance.

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