scholarly journals Bacterial persistence is essential for susceptible cell survival in indirect resistance, mainly for lower cell densities

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0246500
Author(s):  
João S. Rebelo ◽  
Célia P. F. Domingues ◽  
Francisca Monteiro ◽  
Teresa Nogueira ◽  
Francisco Dionisio
Keyword(s):  
Power Law ◽  
Initial Density ◽  
Toxic Substance ◽  
Power Law Distribution ◽  
Persister Cells ◽  
Experimental Conditions ◽  
Power Law Decay ◽  
Cell Densities ◽  
Antibiotic Degradation ◽  
The Impact

Antibiotic-susceptible bacteria may survive bactericidal antibiotics if other co-inhabiting bacteria detoxify the medium through antibiotic degradation or modification, a phenomenon denominated as indirect resistance. However, it is unclear how susceptible cells survive while the medium is still toxic. One explanation relies on the speed of detoxification, and another, non-exclusive explanation, relies on persistence, a state of bacterial dormancy where cells with low metabolic activity and growth rates are phenotypically tolerant to antibiotics and other cytotoxic substances. Here we simulated the fate of susceptible cells in laboratory experiments in the context of indirect resistance to understand whether persistence is necessary to explain the survival of susceptible cells. Depending on the strain and experimental conditions, the decay of persister populations may follow an exponential or a power-law distribution. Therefore, we studied the impact of both distributions in the simulations. Moreover, we studied the impact of considering that persister cells have a mechanism to sense the presence of a toxic substance–a mechanism that would enable cells to leave the dormant state when the medium becomes nontoxic. The simulations show that surviving susceptible cells under indirect resistance may originate both from persister and non-persister populations if the density of detoxifying cells is high. However, persistence was necessary when the initial density of detoxifying cells was low, although persister cells remained in that dormancy state for just a few hours. Finally, the results of our simulations are consistent both with exponential and power-law decay of the persistence population. Whether indirect resistance involves persistence should impact antibiotic treatments.

scholarly journals Phase Synchronization Stability of Non-Homogeneous Low-Voltage Distribution Networks with Large-Scale Distributed Generations

Energies ◽  
2020 ◽  
Vol 13 (5) ◽  
pp. 1257 ◽  
Author(s):  
Shi Chen ◽  
Hong Zhou ◽  
Jingang Lai ◽  
Yiwei Zhou ◽  
Chang Yu
Keyword(s):  
Power Law ◽  
Coupling Strength ◽  
Large Scale ◽  
Phase Synchronization ◽  
Distribution Networks ◽  
Critical Coupling ◽  
Power Law Distribution ◽  
Distributed Generations ◽  
Strength Formula ◽  
The Impact

The ideal distributed network composed of distributed generations (DGs) has unweighted and undirected interactions which omit the impact of the power grid structure and actual demand. Apparently, the coupling relationship between DGs, which is determined by line impedance, node voltage, and droop coefficient, is generally non-homogeneous. Motivated by this, this paper investigates the phase synchronization of an islanded network with large-scale DGs in a non-homogeneous condition. Furthermore, we explicitly deduce the critical coupling strength formula for different weighting cases via the synchronization condition. On this basis, three cases of Gaussian distribution, power-law distribution, and frequency-weighted distribution are analyzed. A synthetical analysis is also presented, which helps to identify the order parameter. Finally, this paper employs the numerical simulation methods to test the effectiveness of the critical coupling strength formula and the superiority over the power-law distribution.

scholarly journals Impact of Bursty Human Activity Patterns on the Popularity of Online Content

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Qiang Yan ◽  
Lianren Wu
Keyword(s):  
Power Law ◽  
Activity Patterns ◽  
Temporal Analysis ◽  
Time Interval ◽  
Online Content ◽  
Power Law Decay ◽  
Content Popularity ◽  
Human Activity Patterns ◽  
The Impact ◽  
Statistical Results

The dynamics of online content popularity has attracted more and more researches in recent years. In this paper, we provide a quantitative, temporal analysis about the dynamics of online content popularity in a massive system: Sina Microblog. We use time-stamped data to investigate the impact of bursty human comment patterns on the popularity of online microblog news. Statistical results indicate that the number of news and comments exhibits an exponential growth. The strength of forwarding and comment is characterized by bursts, displaying fat-tailed distribution. In order to characterize the dynamics of popularity, we explore the distribution of the time intervalΔtbetween consecutive comment bursts and find that it also follows a power-law. Bursty patterns of human comment are responsible for the power-law decay of popularity. These results are well supported by both the theoretical analysis and empirical data.

scholarly journals A dynamic power-law sexual network model of gonorrhoea outbreaks

2018 ◽  
Author(s):  
Lilith K Whittles ◽  
Peter J White ◽  
Xavier Didelot
Keyword(s):  
Network Model ◽  
Power Law ◽  
Dynamic Network ◽  
Great Promise ◽  
Model Parameters ◽  
Power Law Distribution ◽  
Time Model ◽  
Dynamic Network Model ◽  
The Impact ◽  
Over Time

AbstractHuman networks of sexual contacts are dynamic by nature, with partnerships forming and breaking continuously over time. Sexual behaviours are also highly heterogeneous, so that the number of partners reported by individuals over a given period of time is typically distributed as a power-law. Both the dynamism and heterogeneity of sexual partnerships are likely to have an effect in the patterns of spread of sexually transmitted diseases. To represent these two fundamental properties of sexual networks, we developed a stochastic process of dynamic partnership formation and dissolution, which results in power-law numbers of partners over time. Model parameters can be set to produce realistic conditions in terms of the exponent of the power-law distribution, of the number of individuals without relationships and of the average duration of relationships. Using an outbreak of antibiotic resistant gonorrhoea amongst men have sex with men as a case study, we show that our realistic dynamic network exhibits different properties compared to the frequently used static networks or homogeneous mixing models. We also consider an approximation to our dynamic network model in terms of a much simpler branching process. We estimate the parameters of the generation time distribution and offspring distribution which can be used for example in the context of outbreak reconstruction based on genomic data. Finally, we investigate the impact of a range of interventions against gonorrhoea, including increased condom use, more frequent screening and immunisation, concluding that the latter shows great promise to reduce the burden of gonorrhoea, even if the vaccine was only partially effective or applied to only a random subset of the population.

A comparison of two alternative approaches to modelling the sea ice floe size distribution.

2020 ◽  
Author(s):  
Adam Bateson ◽  
Daniel Feltham ◽  
David Schröder ◽  
Lucia Hosekova ◽  
Jeff Ridley ◽  
...  
Keyword(s):  
Sea Ice ◽  
Size Distribution ◽  
Power Law ◽  
Spatial Scales ◽  
Thickness Distribution ◽  
Power Law Distribution ◽  
Momentum Exchange ◽  
Alternative Approaches ◽  
The Impact ◽  
Large Response

<p>Sea ice exists as individual units of ice called floes. These floes can vary by orders of magnitude in diameter over small spatial scales. They are better described by a floe size distribution (FSD) rather than by a single diameter. Observations of the FSD are frequently fitted to a power law with a negative exponent. Floe size can influence several sea ice processes including the lateral melt rate, momentum exchange between the sea ice, ocean and atmosphere, and sea ice rheology. There have been several recent efforts to develop a model of the floe size distribution to include within sea ice models to improve the representation of floe size beyond a fixed single value. Some of these involve significant approximations about the shape and variability of the distribution whereas others adopt a more prognostic approach that does not restrict the shape of the distribution.</p><p>In this study we compare the impacts of two alternative approaches to modelling the FSD within the CICE sea ice model. The first assumes floes follow a power law distribution with a constant exponent. Parameterisations of processes thought to influence the floe size distribution are expressed in terms of a variable FSD tracer. The second uses a prognostic floe size-thickness distribution. The sea ice area in individual floe size categories evolves independently such that the shape of distribution is an emergent behaviour rather than imposed. Here we compare the impact of the two modelling approaches on the thermodynamic evolution of the sea ice. We show that both predict an increase in lateral melt with a compensating reduction in basal melt. We find that the magnitude of this change is highly dependent on the form of the distribution for the smallest floes. We also explore the impact of both FSD models on the momentum exchange of the sea ice and find a large response in the spatial distribution of sea ice volume. Finally, we will discuss whether the results from the prognostic FSD model support the assumptions required to construct the power law derived FSD model.</p>

Uncovering urban mobility patterns and impact of spatial distribution of places on movements

2017 ◽  
Vol 28 (01) ◽  
pp. 1750004 ◽  
Author(s):  
Wang Chen ◽  
Qiang Gao ◽  
Hua-Gang Xiong
Keyword(s):  
Spatial Distribution ◽  
Power Law ◽  
Urban Areas ◽  
Large Scale ◽  
Length Distribution ◽  
Distribution Area ◽  
Urban Mobility ◽  
Mobility Patterns ◽  
Power Law Decay ◽  
The Impact

As an important component in varieties of practical applications, understanding human urban mobility patterns draws intensive attention from researchers. In this paper, we investigate the urban mobility patterns and the impact of spatial distribution of places on the patterns using the data from a popular location-based social network Whrrl which are unrestricted to transportation modes. A movement region is demarcated for each city, which better depicts the concentrated active area of residents in the city than the administrative region. We show that the trip lengths in urban areas follow the exponential law unlike the power law in large scale of space. We find that the cities with larger sizes of place distribution area generally have smaller exponents of trip length distribution, larger means and deviations of trip lengths, while there are no apparent relationships between place densities and trip lengths. To examine the findings, we construct series of synthetic cities based on the power-law decay of place density and simulate urban human movement by the rank-based model. The simulations validate our findings and imply that the exponential distribution of urban trips is a combined result of power-law decay of place density and rank-based mobility preference.

Experimental Study of the Impact of Alisma plantago-aquatica Secretions on Pathogenic Bacteria

2014 ◽  
Vol 1 (3) ◽  
pp. 3-7
Author(s):  
O. Zhukorskyy ◽  
O. Hulay
Keyword(s):  
Pathogenic Bacteria ◽  
Initial Density ◽  
Biologically Active ◽  
Erysipelothrix Rhusiopathiae ◽  
Natural Focus ◽  
Test Species ◽  
Popula Tions ◽  
And Control ◽  
The Impact

Aim. To estimate the impact of in vivo secretions of water plantain (Alisma plantago-aquatica) on the popula- tions of pathogenic bacteria Erysipelothrix rhusiopathiae. Methods. The plants were isolated from their natural conditions, the roots were washed from the substrate residues and cultivated in laboratory conditions for 10 days to heal the damage. Then the water was changed; seven days later the selected samples were sterilized using fi lters with 0.2 μm pore diameter. The dilution of water plantain root diffusates in the experimental samples was 1:10–1:10,000. The initial density of E. rhusiopathiae bacteria populations was the same for both experimental and control samples. The estimation of the results was conducted 48 hours later. Results. When the dilution of root diffusates was 1:10, the density of erysipelothrixes in the experimental samples was 11.26 times higher than that of the control, on average, the dilution of 1:100 − 6.16 times higher, 1:1000 – 3.22 times higher, 1:10,000 – 1.81 times higher, respectively. Conclusions. The plants of A. plantago-aquatica species are capable of affecting the populations of E. rhusiopathiae pathogenic bacteria via the secretion of biologically active substances into the environment. The consequences of this interaction are positive for the abovementioned bacteria, which is demon- strated by the increase in the density of their populations in the experiment compared to the control. The intensity of the stimulating effect on the populations of E. rhusiopathiae in the root diffusates of A. plantago-aquatica is re- ciprocally dependent on the degree of their dilution. The investigated impact of water plantain on erysipelothrixes should be related to the topical type of biocenotic connections, the formation of which between the test species in the ecosystems might promote maintaining the potential of natural focus of rabies. Keywords: Alisma plantago-aquatica, in vivo secretions, Erysipelothrix rhusiopathiae, population density, topical type of connections.

scholarly journals Glucuronides Hydrolysis by Intestinal Microbial β-Glucuronidases (GUS) Is Affected by Sampling, Enzyme Preparation, Buffer pH, and Species

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1043
Author(s):  
Christabel Ebuzoeme ◽  
Imoh Etim ◽  
Autumn Ikimi ◽  
Jamie Song ◽  
Ting Du ◽  
...  
Keyword(s):  
Enzyme Preparation ◽  
Reaction System ◽  
Reaction Rates ◽  
Experimental Conditions ◽  
Model Animal ◽  
Fresh Feces ◽  
Time Buffer ◽  
Exogenous Compounds ◽  
Activity Loss ◽  
The Impact

Glucuronides hydrolysis by intestinal microbial β-Glucuronidases (GUS) is an important procedure for many endogenous and exogenous compounds. The purpose of this study is to determine the impact of experimental conditions on glucuronide hydrolysis by intestinal microbial GUS. Standard probe 4-Nitrophenyl β-D-glucopyranoside (pNPG) and a natural glucuronide wogonoside were used as the model compounds. Feces collection time, buffer conditions, interindividual, and species variations were evaluated by incubating the substrates with enzymes. The relative reaction activity of pNPG, reaction rates, and reaction kinetics for wogonoside were calculated. Fresh feces showed the highest hydrolysis activities. Sonication increased total protein yield during enzyme preparation. The pH of the reaction system increased the activity in 0.69–1.32-fold, 2.9–12.9-fold, and 0.28–1.56-fold for mouse, rat, and human at three different concentrations of wogonoside, respectively. The Vmax for wogonoside hydrolysis was 2.37 ± 0.06, 4.48 ± 0.11, and 5.17 ± 0.16 μmol/min/mg and Km was 6.51 ± 0.71, 3.04 ± 0.34, and 0.34 ± 0.047 μM for mouse, rat, and human, respectively. The inter-individual difference was significant (4–6-fold) using inbred rats as the model animal. Fresh feces should be used to avoid activity loss and sonication should be utilized in enzyme preparation to increase hydrolysis activity. The buffer pH should be appropriate according to the species. Inter-individual and species variations were significant.

scholarly journals The structure of co-publications multilayer network

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Ghislain Romaric Meleu ◽  
Paulin Yonta Melatagia
Keyword(s):  
Power Law ◽  
Degree Distribution ◽  
Preferential Attachment ◽  
Small World ◽  
Generation Model ◽  
Small World Network ◽  
Power Law Distribution ◽  
Multilayer Networks ◽  
Multilayer Network ◽  
Scientific Papers

AbstractUsing the headers of scientific papers, we have built multilayer networks of entities involved in research namely: authors, laboratories, and institutions. We have analyzed some properties of such networks built from data extracted from the HAL archives and found that the network at each layer is a small-world network with power law distribution. In order to simulate such co-publication network, we propose a multilayer network generation model based on the formation of cliques at each layer and the affiliation of each new node to the higher layers. The clique is built from new and existing nodes selected using preferential attachment. We also show that, the degree distribution of generated layers follows a power law. From the simulations of our model, we show that the generated multilayer networks reproduce the studied properties of co-publication networks.

scholarly journals Power-law behavior of transcription factor dynamics at the single-molecule level implies a continuum affinity model

2021 ◽  
Author(s):  
David A Garcia ◽  
Gregory Fettweis ◽  
Diego M Presman ◽  
Ville Paakinaho ◽  
Christopher Jarzynski ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Single Molecule ◽  
Power Law ◽  
Dwell Time ◽  
Specific Binding ◽  
Power Law Distribution ◽  
Single Molecule Level ◽  
Binding Behavior ◽  
Chromatin Template ◽  
Nuclear Domains

Abstract Single-molecule tracking (SMT) allows the study of transcription factor (TF) dynamics in the nucleus, giving important information regarding the diffusion and binding behavior of these proteins in the nuclear environment. Dwell time distributions obtained by SMT for most TFs appear to follow bi-exponential behavior. This has been ascribed to two discrete populations of TFs—one non-specifically bound to chromatin and another specifically bound to target sites, as implied by decades of biochemical studies. However, emerging studies suggest alternate models for dwell-time distributions, indicating the existence of more than two populations of TFs (multi-exponential distribution), or even the absence of discrete states altogether (power-law distribution). Here, we present an analytical pipeline to evaluate which model best explains SMT data. We find that a broad spectrum of TFs (including glucocorticoid receptor, oestrogen receptor, FOXA1, CTCF) follow a power-law distribution of dwell-times, blurring the temporal line between non-specific and specific binding, suggesting that productive binding may involve longer binding events than previously believed. From these observations, we propose a continuum of affinities model to explain TF dynamics, that is consistent with complex interactions of TFs with multiple nuclear domains as well as binding and searching on the chromatin template.

scholarly journals Searching for energy-resolved quasi-periodic oscillations in AGN

2021 ◽  
Author(s):  
Dominic I Ashton ◽  
Matthew J Middleton
Keyword(s):  
Power Law ◽  
Spectral Component ◽  
Broad Band ◽  
Initial Study ◽  
Periodic Oscillations ◽  
Single Observation ◽  
Curved Space ◽  
Multiple Observations ◽  
Band Noise ◽  
The Impact

Abstract X-ray quasi-periodic oscillations (QPOs) in AGN allow us to probe and understand the nature of accretion in highly curved space-time, yet the most robust form of detection (i.e. repeat detections over multiple observations) has been limited to a single source to-date, with only tentative claims of single observation detections in several others. The association of those established AGN QPOs with a specific spectral component has motivated us to search the XMM-Newton archive and analyse the energy-resolved lightcurves of 38 bright AGN. We apply a conservative false alarm testing routine folding in the uncertainty and covariance of the underlying broad-band noise. We also explore the impact of red-noise leak and the assumption of various different forms (power-law, broken power-law and lorentzians) for the underlying broad-band noise. In this initial study, we report QPO candidates in 6 AGN (7 including one tentative detection in MRK 766) from our sample of 38, which tend to be found at characteristic energies and, in four cases, at the same frequency across at least two observations, indicating they are highly unlikely to be spurious in nature.

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