Queen honey bees exhibit variable resilience to temperature stress

Extreme temperature exposure can reduce stored sperm viability within queen honey bees; however, little is known about how thermal stress may directly impact queen performance or other maternal quality metrics. Here, in a blind field trial, we recorded laying pattern, queen mass, and average callow worker mass before and after exposing queens to a cold temperature (4°C, 2 h), hot temperature (42°C, 2 h), and hive temperature (33°C, control). We measured sperm viability at experiment termination, and investigated potential vertical effects of maternal temperature stress on embryos using proteomics. We found that cold stress, but not heat stress, reduced stored sperm viability; however, we found no significant effect of temperature stress on any other recorded metrics (queen mass, average callow worker mass, laying patterns, the egg proteome, and queen spermathecal fluid proteome). Previously determined candidate heat and cold stress biomarkers were not differentially expressed in stressed queens, indicating that these markers only have short-term post-stress diagnostic utility. Combined with variable sperm viability responses to temperature stress reported in different studies, these data also suggest that there is substantial variation in temperature tolerance, with respect to impacts on fertility, amongst queens. Future research should aim to quantify the variation and heritability of temperature tolerance, particularly heat, in different populations of queens in an effort to promote queen resilience.

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Transcriptome analysis of the Larimichthys polyactis under heat and cold stress

10.1101/779462 ◽

2019 ◽

Author(s):

Tianqi Chu ◽

Feng Liu ◽

Gaochan Qin ◽

Wei Zhan ◽

Mengjie Wang ◽

...

Keyword(s):

Cold Stress ◽

Temperature Stress ◽

Large Scale ◽

Molecular Mechanisms ◽

Control Group ◽

Pathway Enrichment Analysis ◽

Larimichthys Polyactis ◽

Small Yellow Croaker ◽

Heat And Cold Stress ◽

Temperature Group

AbstractThe small yellow croaker (Larimichthys polyactis) is an important marine economic fish that is widely distributed in the East Sea, Yellow Sea and Bohai of China. However, the wild populations of small yellow croaker are severely depleted, and there is currently a developing large-scale artificial propagation of this fish for aquaculture. However, the current variety of small yellow croaker that is cultivated is not capable to coping with large fluctuations in temperature. Therefore, it is important to understand the molecular mechanisms that are activated in response to temperature stress in the small yellow croaker. Here, we conducted transcriptomic analysis of the liver of small yellow croaker under heat and cold stress. A total of 270,844,888, 265,727,006 and 259,666,218 clean reads were generated from heat temperature group, low temperature group and control group, respectively, and comparing expression of genes in these transcriptomes,10,878 unigenes that were differential expressed were identified. Sixteen of the differentially expressed unigenes were validated by qRT-PCR. Pathway enrichment analysis identified that the ER pathway, immune signaling pathway and metabolic response pathway were affected by temperature stress. The results of this study provide a comprehensive overview of temperature stress-induced transcriptional patterns in liver tissues of the small yellow croaker. In addition, these results can guide future molecular studies of heat and cold stress response in this species for improving the stock used for aquaculture.

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Effect of Temperature on Physical and Optical Properties of Photoelastic Materials

Journal of Applied Mechanics ◽

10.1115/1.4008820 ◽

1938 ◽

Vol 5 (1) ◽

pp. A11-A12

Author(s):

G. H. Lee ◽

C. W. Armstrong

Keyword(s):

Heat Treatment ◽

Optical Properties ◽

Temperature Stress ◽

Poisson’S Ratio ◽

Poisson's Ratio ◽

Effect Of Temperature ◽

Stress Strain ◽

Optical Coefficient ◽

Tension Tests ◽

Before And After

Abstract This paper reports the results of tension tests, to determine the suitability for photoelastic work, of five samples of Bakelite and two of Marblette at temperatures between 32 and 140 F. These results show the variation, of Young’s modulus, Poisson’s ratio, stress-optical coefficient, and strain-optical coefficient with temperature. Stress-strain curves of the materials are also given showing the relative amounts of creep. The effect of heat-treatment on Marblette is shown by tests before and after annealing.

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Queens, but not their stored sperm, are resilient to temperature stress

10.21203/rs.3.rs-280020/v1 ◽

2021 ◽

Author(s):

Alison McAfee ◽

David R Tarpy ◽

Leonard J Foster

Keyword(s):

Temperature Stress ◽

Honey Bees ◽

Egg Laying ◽

Extreme Temperatures ◽

Protein Markers ◽

Abiotic Stressors ◽

Before And After ◽

Effects Of Temperature ◽

Honey Bee Queens ◽

Maternal Quality

Abstract Abiotic stressors such as extreme temperatures can reduce stored sperm viability within queen honey bees. However, little is known about how thermal stress may directly impact queen performance or other maternal quality metrics, such as queen mass, egg laying rate, and development of embryos within eggs. Here, in a blind field trial, we recorded laying pattern, queen mass, and average callow worker mass before and after exposing queens to a cold temperature (4°C, 2 h), hot temperature (42°C, 2 h), and hive temperature (33°C) to serve as a handling control. We then used proteomics to investigate potential vertical effects of maternal temperature stress on embryos, as well as to measure the abundance of previously determined protein markers for temperature stress in the spermathecal fluid. We found no significant effect of abiotic stress on any of the metrics we recorded. These data suggest that there are likely no lasting maternal effects of temperature stress on honey bee queens, and that the queens themselves are highly stress tolerant, but not their stored sperm.

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Dealing with hot rocky environments: Critical thermal maxima and locomotor performance in Leptodactylus lithonaetes (Anura: Leptodactylidae)

Herpetological Journal ◽

10.33256/hj29.3.155161 ◽

2019 ◽

pp. 155-161 ◽

Cited By ~ 1

Author(s):

Ivan Beltran

Keyword(s):

Environmental Temperature ◽

Extreme Temperature ◽

Effect Of Temperature ◽

Maximum Speed ◽

Locomotor Performance ◽

Extreme Temperatures ◽

Physiological Limits ◽

Fitness Consequences ◽

Thermal Maximum ◽

Environmental Temperatures

Environmental temperature has fitness consequences on ectotherm development, ecology and behaviour. Amphibians are especially vulnerable because thermoregulation often trades with appropriate water balance. Although substantial research has evaluated the effect of temperature in amphibian locomotion and physiological limits, there is little information about amphibians living under extreme temperature conditions. Leptodactylus lithonaetes is a frog allegedly specialised to forage and breed on dark granitic outcrops and associated puddles, which reach environmental temperatures well above 40 ˚C. Adults can select thermally favourable microhabitats during the day while tadpoles are constrained to rock puddles and associated temperature fluctuations; we thus established microhabitat temperatures and tested whether the critical thermal maximum (CTmax) of L. lithonaetes is higher in tadpoles compared to adults. In addition, we evaluated the effect of water temperature on locomotor performance of tadpoles. Contrary to our expectations, puddle temperatures were comparable and even lower than those temperatures measured in the microhabitats used by adults in the daytime. Nonetheless, the CTmax was 42.3 ˚C for tadpoles and 39.7 ˚C for adults. Regarding locomotor performance, maximum speed and maximum distance travelled by tadpoles peaked around 34 ˚C, approximately 1 ˚C below the maximum puddle temperatures registered in the puddles. In conclusion, L. lithonaetes tadpoles have a higher CTmax compared to adults, suggesting a longer exposure to extreme temperatures that lead to maintain their physiological performance at high temperatures. We suggest that these conditions are adaptations to face the strong selection forces driven by this granitic habitat.

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BIOCLIMATES OF HEAT AND COLD STRESS IN NEW ZEALAND

Weather and Climate ◽

10.2307/44279738 ◽

1987 ◽

Vol 7 (2) ◽

pp. 55 ◽

Cited By ~ 9

Author(s):

de Freitas

Keyword(s):

New Zealand ◽

Cold Stress ◽

Heat And Cold Stress

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Influences of Stress on Interactions with Decision Support

Proceedings of the Human Factors and Ergonomics Society Annual Meeting ◽

10.1177/1071181320641454 ◽

2020 ◽

Vol 64 (1) ◽

pp. 1886-1890

Author(s):

Kylie Litaker ◽

Christopher B. Mayhorn

Keyword(s):

Decision Support ◽

Information Source ◽

Negative Influence ◽

Future Research ◽

Physiological Measures ◽

Task Motivation ◽

Situational Stress ◽

Before And After ◽

Scanning Task ◽

Source Of Information

People regularly interact with automation to make decisions. Research shows that reliance on recommendations can depend on user trust in the decision support system (DSS), the source of information (i.e. human or automation), and situational stress. This study explored how information source and stress affect trust and reliance on a DSS used in a baggage scanning task. A preliminary sample of sixty-one participants were given descriptions for a DSS and reported trust before and after interaction. The DSS gave explicit recommendations when activated and participants could choose to rely or reject the choice. Results revealed a bias towards self-reliance and a negative influence of stress on trust, particularly for participants receiving help from automation. Controlling for perceived reliability may have eliminated trust biases prior to interaction, while stress may have influenced trust during the task. Future research should address potential differences in task motivation and include physiological measures of stress.

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Acquiring variable commands at home and abroad: Examining optatives and imperatives in L1 and L2 Spanish

Studies in Hispanic and Lusophone Linguistics ◽

10.1515/shll-2020-2025 ◽

2020 ◽

Vol 13 (1) ◽

pp. 79-113

Author(s):

Farrah Neumann ◽

Matthew Kanwit

Keyword(s):

Second Language ◽

Second Language Acquisition ◽

Study Abroad ◽

Language Teaching ◽

Applied Linguistics ◽

Future Research ◽

Control Group ◽

Before And After ◽

The Individual ◽

At Home

AbstractSince many linguistic structures are variable (i. e. conveyed by multiple forms), building a second-language grammar critically involves developing sociolinguistic competence (Canale and Swain. 1980. Theoretical bases of communicative approaches to second language teaching and testing. Applied Linguistics 1(1). 1–47), including knowledge of contexts in which to use one form over another (Bayley and Langman. 2004. Variation in the group and the individual: Evidence from second language acquisition. International Review of Applied Linguistics in Language Teaching 42(4). 303–318). Consequently, researchers interested in such competence have increasingly analyzed the study-abroad context to gauge learners’ ability to approximate local norms following a stay abroad, due to the quality and quantity of input to which learners may gain access (Lafford. 2006. The effects of study abroad vs. classroom contexts on Spanish SLA: Old assumptions, new insights and future research directions. In Carol Klee & Timothy Face (eds.), Selected proceedings of the 7th conference on the acquisition of Spanish and Portuguese as first and second languages, 1–25. Somerville, MA: Cascadilla Proceedings Project). Nevertheless, the present study is the first to examine native or learner variation between imperative (e. g. ven ‘come’) and optative Spanish commands (e. g. que vengas ‘come’). We first performed a corpus analysis to determine the linguistic factors to manipulate in a contextualized task, which elicited commands from learners before and after four weeks abroad in Alcalá de Henares, Spain. Their overall rates of selection and predictive factors were compared to local native speakers (NSs) and a control group of at-home learners.Results revealed that the abroad learners more closely approached NS rates of selection following the stay abroad. Nonetheless, for both learner groups conditioning by independent variables only partially approximated the NS system, which was more complex than previously suggested.

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How Collective-Action Failure Shapes Group Heterogeneity and Engagement in Conventional and Radical Action Over Time

Psychological Science ◽

10.1177/0956797620970562 ◽

2021 ◽

pp. 095679762097056

Author(s):

Morgana Lizzio-Wilson ◽

Emma F. Thomas ◽

Winnifred R. Louis ◽

Brittany Wilcockson ◽

Catherine E. Amiot ◽

...

Keyword(s):

Social Movements ◽

Collective Action ◽

Latent Profile Analysis ◽

Profile Analysis ◽

Social Identification ◽

Future Research ◽

Group Efficacy ◽

Before And After ◽

Using Data ◽

Over Time

Extensive research has identified factors influencing collective-action participation. However, less is known about how collective-action outcomes (i.e., success and failure) shape engagement in social movements over time. Using data collected before and after the 2017 marriage-equality debate in Australia, we conducted a latent profile analysis that indicated that success unified supporters of change ( n = 420), whereas failure created subgroups among opponents ( n = 419), reflecting four divergent responses: disengagement (resigned acceptors), moderate disengagement and continued investment (moderates), and renewed commitment to the cause using similar strategies (stay-the-course opponents) or new strategies (innovators). Resigned acceptors were least inclined to act following failure, whereas innovators were generally more likely to engage in conventional action and justify using radical action relative to the other profiles. These divergent reactions were predicted by differing baseline levels of social identification, group efficacy, and anger. Collective-action outcomes dynamically shape participation in social movements; this is an important direction for future research.

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Stock Market Reaction to COVID-19: Evidence in Customer Goods Sector with the Implication for Open Innovation

Journal of Open Innovation Technology Market and Complexity ◽

10.3390/joitmc6040099 ◽

2020 ◽

Vol 6 (4) ◽

pp. 99

Author(s):

Zaky Machmuddah ◽

St. Dwiarso Utomo ◽

Entot Suhartono ◽

Shujahat Ali ◽

Wajahat Ali Ghulam

Keyword(s):

Stock Prices ◽

Stock Price ◽

Practical Implication ◽

Efficient Market Hypothesis ◽

Future Research ◽

Observation Data ◽

Economic Sectors ◽

Significant Difference ◽

Before And After ◽

Long Term Impact

The coronavirus pandemic has spread all over the world, affecting both the health and economic sectors. The aim of this research was to observe stock prices of customer goods before and after the COVID-19 pandemic using event study and the comparison test. The sample included data of daily closing stock prices and volume of stock trade during the three months before (−90 days) and after (+90 days) the occurrence of the COVID-19 pandemic ongoing, totaling 2670 observation data both before and after the COVID-19 pandemic, for a total of 5340. The research findings indicate a significant difference between the daily closing stock price and volume of stock trade before and after the COVID-19 pandemic. The current research has both theoretical and practical implications: the findings strengthen the efficient market hypothesis, which states that the more complete the provided information, the more efficient the market. The practical implication is that investors should be careful when choosing to invest. Investors should choose customer goods sector companies that provide products that are much needed by customers, for example, pharmacy, food, beverages, etc. Future research is needed to investigate the long-term impact of the pandemic on the economy.

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