scholarly journals Effect of shell as natural testosterone boosters in Sprague Dawley rats

2019 ◽  
Vol 12 (10) ◽  
pp. 1677-1681
Author(s):  
Pudji Astuti ◽  
Claude Mona Airin ◽  
Sarmin Sarmin ◽  
Alfarisa Nururrozi ◽  
Sri Harimurti
Keyword(s):  
Testosterone Level ◽  
Male Reproduction ◽  
Male Rats ◽  
Sprague Dawley ◽  
Testosterone Levels ◽  
Green Mussel ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
Blood Clam ◽  
Conch Shell

Aim: This study aimed to evaluate the effect of shell supplementation on the regulation of male reproduction in rats. Materials and Methods: The zinc (Zn) level of shell from blood clam (Anadara granosa), green mussel (Perna viridis), and conch shell (Telescopium telescopium) was analyzed. The highest Zn content shell was fed to male Sprague Dawley rats for 0, 9, 30, and 50 days at the dose of either 0.09 mg/200 g BW or 0.18 mg/200 g BW. To determine the testosterone levels, blood was collected through the infraorbitalis sinus just before the rat was sacrificed. Testicular and brain were also collected for Cyp19 aromatase receptor analysis. Results: The Zn level in the shell of blood clam, green mussel, and conch shell 61.55 mg/kg, 2.78 mg/kg, and 3.93 mg/kg, respectively. The testosterone level of T1 group receiving 0.18 mg/200 g BW for 0, 9, 30, and 50 days was 1.42±0.59, 2.15±1.58, 2.98±2.53, and 8.11±2.03 ng/mL, respectively. The testosterone level of T2 group receiving 0.09 mg/200 g BW for 0, 9, 30, and 50 days was 2.50±0.32, 1.25±0.60, 3.87±3.27, and 3.54±0.23 ng/mL, respectively. The T3 group receiving Na-CMC showed the level of testosterone at days 0, 9, 30, and 50 days was 0.77±0.22, 1.99±1.65, 4.12±0.07, and 2.19±1.30 ng/mL, respectively. Finally, the T4 group receiving Zn showed testosterone levels at days 0, 9, 30, and 50 days was 0.51±0.58, 2.24±3.16, 4.58±1.97, and 2.89±0.20 ng/mL, respectively. There was a significant difference (p<0.05) between the T1 group compared to the other groups. However, the absence of expression of Cyp19 aromatase both in Leydig cells and the brain indicated no conversion of testosterone to estradiol. To add, this finding showed the potential use of the shell to boost the testosterone level in male rats. Conclusion: Shell acted as an aromatase blocker to boost the testosterone level in male rats. This also indicates its promising application in birds to manipulate the quality of song and feather.

scholarly journals The Effects of Chronic Ingestion of Mercuric Chloride on Fertility and Testosterone Levels in Male Sprague Dawley Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
John C. Heath ◽  
Y. Abdelmageed ◽  
Tim D. Braden ◽  
Hari O. Goyal
Keyword(s):  
Mercuric Chloride ◽  
Inorganic Mercury ◽  
Male Reproduction ◽  
Male Rats ◽  
Male Rat ◽  
Sprague Dawley ◽  
Testosterone Levels ◽  
Mercury Compounds ◽  
Sprague Dawley Rats ◽  
Sperm Counts

Although male infertility is well researched, the effects of inorganic mercury on male reproduction and fertility are less well known. Studies pertaining to mercury and male fertility identified reduced concentration of testosterone in the serum of male workers, a toxic influence on fertility of organic mercury compounds within concentrations at the workplace, and increased days to pregnancy. We evaluated the effect of chronic mercuric chloride (HgCl2) exposure in male rats on reproductive endpoints. Thirty-day old male Sprague Dawley rats (n=31) were exposed to 0.0, 1.0, or 2.0 mg/kg/day of HgCl2via gavage. After 60 days exposure, they were housed with nonexposed females for 21 days. A survivor analysis revealed the exposed animals took longer to impregnate the females and had a lower rate of impregnation. Further statistical analysis revealed a lower correlation between testicular testosterone levels and days to impregnate, and also lower sperm counts in the epididymis head and body of the exposed males. The results indicate that HgCl2exposure had significant adverse effects on male rat reproduction endpoints including fertility at a dose that was not clinically toxic.

scholarly journals Eurycoma longifolia Root Aqueous Extract Augments the Testosterone Level and Spermatogenesis in Adult Male Rats

2020 ◽  
Vol 18 (3) ◽  
Author(s):  
Hamoud H. Al-Faqeh ◽  
Mohammed Imad AMM
Keyword(s):  
Aqueous Extract ◽  
Adult Male ◽  
Testosterone Level ◽  
Male Rats ◽  
Control Group ◽  
Distilled Water ◽  
Sprague Dawley ◽  
Testosterone Levels ◽  
Eurycoma Longifolia ◽  
Healthy Adult Male

Introduction The Eurycoma longifolia (EL) root aqueous extract has long been used as an enhancer of male sexual performance. However, data from previous studies in both human males and experimental male animals on the testosterone level in those given the EL extract orally were at best insufficiently conclusive. Materials and Method Sixty-four healthy adult male Sprague Dawley rats were acclimatized, and randomized into six test groups and one control group. All rats where given either the aqueous EL extract or distilled water via metal gavage needle. The first three test groups received the low (50mg/kg bw), medium (100mg/ kg bw) and high (200mg/kg bw) doses respectively of the EL daily for 15 days only. The second three test groups continued receiving the same daily treatment doses for 30 days. The controls were given distilled water only. At the end of each of the study period, blood samples were collected via cardiac puncture and the rats were euthanized. The testicles were obtained, weighed, and processed for histological examination. Results The sera testosterone levels were higher in animals which received the medium and high doses for both treatment duration. Rats which received medium and high oral doses of EL showed an increase of spermatogenesis and mature spermatozoa. Conclusion The optimal enhancing effect on sera testosterone levels and testicular spermatogenesis of EL treatment in adult male rats was observed with the medium dose of 100mg/kg bw given once daily for both 15 and 30 days.

Ratio Kadar Ldl/Hdl Tikus Putih Sprague Dawley Hiperlipidemia Setelah Diberi Cangkang Udang Laut (Penaeus monodon F.)

2015 ◽  
Vol 17 (2) ◽  
pp. 118
Author(s):  
Sri Isdadiyanto
Keyword(s):  
Body Weight ◽  
Penaeus Monodon ◽  
Male Rats ◽  
Sprague Dawley ◽  
High Fat ◽  
Group Iii ◽  
Group I ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
The Difference

The objective of this study was to analyze the ratio of LDL/HDL level of Sprague Dawley rats induced by high fat ration after given of sea shrimp carapace (Penaeus monodon F.). The animals for this study were twenty adult male rats divided into four groups, i.e. group I as the control was fed with basal ration containing normal fat for 3 months, group II was fed ration containing high fat for 3 months, group III was fed ration containing high fat and given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 3 months, group IV was fed ration containing high fat for 3 months and after 1 month given sea shrimp carapace 180 mg per kg body weight per day orally in 2 ml aquadest for 2 months. Each group consisted of five animals. After 90 days, the rats were necropsied and the blood were collected to analyzes ratio of LDL/HDL level. LDL level and HDL level were measured using colorimetric enzymatic method and Roche/Hitachi cobas c systems automatically calculate.  The difference between treatments was statistical analysis by Anava, and continued by Lowest Significant Difference Test to locate the difference. The result showed that high fat diet increased ratio of LDL/HDL level and Sea shrimp carapace decreased ratio of LDL/HDL level.   Keywords: Sea shrimp carapace, Sprague Dawley rats, ratio of LDL/HDL level

The growth and antigenicity of the metacestodes of Taenia crassiceps in several different strains of rats

1981 ◽  
Vol 55 (3) ◽  
pp. 209-222 ◽  
Author(s):  
J. Chernin
Keyword(s):  
Female Rats ◽  
Male Rats ◽  
Sprague Dawley ◽  
Taenia Crassiceps ◽  
Male And Female Rats ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
Antigenic Expression ◽  
Outbred Strain ◽  
Different Strains

ABSTRACTThe metacestodes of Taenia crassiceps removed from a stock infection of Sprague Dawley rats were injected into seven inbred and one outbred strain of rats. The results showed that over-all female rats are more susceptible to the infection than male rats. Only one strain of rat, the Lewis male rats, was found to be resistant to the infection. There was no significant difference in the volume of metacestodes recovered from the male and female rats. The sizes of the metacestodes, after two passages through the rats, were compared to those of the original mouse strain and it was found that metacestodes from two strains of rats were significantly smaller. The antigenic expression of the metacestodes from the different strains of rats was compared and some variation in the antigenicity was observed.

scholarly journals Examination of Apoptotic and Autophagic Effects of Chronic Roflumilast Use on Rat Testicular Tissue by Immunohistochemical and Immunofluorescence Methods

2021 ◽  
Author(s):  
arzu gezer ◽  
Ebru KARADAĞ SARI
Keyword(s):  
Testosterone Level ◽  
Caspase 3 ◽  
Serum Testosterone ◽  
Testicular Tissue ◽  
Male Rats ◽  
Pde4 Inhibitor ◽  
Serum Testosterone Level ◽  
Sprague Dawley ◽  
Negative Effects ◽  
Testosterone Levels

Abstract Roflumilast (ROF) (3-cyclo-propylmethoxy-4-difuorome-thoxy-N-[3,5-di-chloropyrid-4-yl] benzamide) is a second generation and forcible phosphodiesterase-4 (PDE4) inhibitor. This study aims to investigate the effects of chronic Roflumilast in different doses on testicular tissue and testosterone levels in healthy Sprague-Dawley rats. During the research, the 6 weeks old (180–200 gr), 36 male rats were divided into 4 groups. Roflumilast (ROF) was administered as 0.5 mg/kg and 1 mg/kg by oral gavage for four weeks, once each day. Hematoxylin-Eosin (H&E) staining for histopathological examinations in testicular tissue, immunohistochemical and immunofluorescence examinations for Caspase-3, Apoptosis Inducing Factor (AIF) and Light Chain 3β (LC3B) expression levels, and ELISA method used to determine serum testosterone levels. Data were analyzed using SPSS v.22 with Kruskal-Wallis, Mann Whitney-U, and Wilcoxon tests. Roflumilast group lost weight compared to the control and sham. Shedding in the seminiferous epithelium, degenerations in the interstitial area, separation between cells, desquamation, interstitial edema and degenerative changes in the testicular tissue was observed. While apoptosis and autophagy determined by Caspase-3, AIF and LC3B were close and statistically insignificant in control and sham, there was significantly increased apoptotic and autophagic changes and immunopositivity in the ROF groups. The 1 mg/kg Roflumilast group’s serum testosterone level was lower than control, sham and 0.5 mg/kg Roflumilast groups. When the research data was evaluated, it was determined that the chronic use of the active ingredient Roflumilast, which has a broad-spectrum area such as COPD, arthritis, neurodegenerative diseases, liver and dermatology, had negative effects on the testicular tissue and testosterone level of rats.

scholarly journals The Effect of Single and Triple Testicular Biopsy Using Biopty Gun on Spermatogenesis in Pubertal Rats

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1569
Author(s):  
Tomislav Šušnjar ◽  
Ivana Kuzmić Prusac ◽  
Ivan Švagelj ◽  
Anđela Jurišić ◽  
Tomislav Šušnjar ◽  
...  
Keyword(s):  
Sperm Count ◽  
Semen Analysis ◽  
Study Groups ◽  
Testicular Biopsy ◽  
Male Rats ◽  
Lower Percentage ◽  
Control Group ◽  
Sprague Dawley ◽  
Left Testis ◽  
Significant Difference

Background: The aim of this study was to compare consequences in single and triple testicular biopsy by biopty gun in pubertal rats using histological and immunohistochemical analysis. Methods: Thirty-two Sprague-Dawley male rats were used as the experimental model. The rats were randomly divided into three study groups. The rats from the first group (n = 12) received a single-biopsy of upper pole of the left testis, while the rats from the second group (n = 10) received triple-biopsy of upper and lower poles and lateral surface of left testis. The third group (n = 10) was a control group. On the eightieth day after the biopsy in all rats bilateral orchiectomy and funiculectomy were performed to obtain testicular tissue and sperm for analysis. The consequences of the puncture were observed by pathohistology, immunohistochemistry and semen analysis. Results: The results of the study showed lower percentage of sperm count (14.5 mill/mL vs. 16 mill/mL, p = 0.130), sperm motility (24.6% vs. 32.7%, p > 0.05), abnormal sperm (30% vs. 27%, p > 0.05), atrophic tubules (21% vs. 6%, p < 0.001), volume (1.7 mL vs. 2.28 mL, p < 0.01) and apoptotic index (1.56 vs. 1.19, p = 0.650) in the testes with a triple-biopsy compared to the testes with a single-biopsy. Semen analysis showed a borderline significant difference between the group with triple-biopsy where sperm count was lower than it in the control group (14.5 mill/mL vs. 17.5 mill/mL, p = 0.05). A single-biopsy has little effect on the testis, especially on overall fertility. A triple-biopsy showed higher degree of the testicular damage but without a significant impact on overall fertility. Semen analysis showed that single- and triple-biopsies did not have a significant effect on sperm count, motility and morphology. Conclusion: Biopty gun procedure is a cheap, simple and reliable method for testicular biopsy in rats without a significant effect on sperm count, motility and morphology.

scholarly journals THE EFFECTS OF LEVELS OF ISOLATION, OR VARIETAL DIFFERENCES IN, HIGH FIBRE HULL FRACTION OF LOW GLUCOSINOLATE RAPESEED MEALS ON RAT OR PIG PERFORMANCE

1978 ◽  
Vol 58 (4) ◽  
pp. 743-752 ◽  
Author(s):  
J. J. KENNELLY ◽  
F. X. AHERNE ◽  
A. J. LEWIS
Keyword(s):  
Feed Intake ◽  
Average Daily Gain ◽  
Rapeseed Meal ◽  
Sprague Dawley ◽  
Crude Fibre ◽  
Gain Ratio ◽  
Sprague Dawley Rats ◽  
Significant Difference ◽  
Pig Performance ◽  
Daily Gain

Forty-eight crossbred pigs of average initial weight 21 kg were fed 10% Tower rapeseed meal (RSM) and 10% Candle RSM as partial replacements for soybean meal (SBM). Diets were formulated to be isocaloric. Pigs fed the SBM diet consumed less feed, gained significantly (P < 0.01) faster and were more efficient at converting feed to gain than those fed the RSM diets. Performance of pigs fed Candle RSM was not significantly different to that obtained with Tower RSM. In a second experiment, dehulled Tower RSM and Tower RSM hulls were mixed in amounts to produce RSM with crude fibre levels of 6.8, 10.8, 13.5 and 15.8%. The simulated RSM and Tower and Candle RSM were used to completely replace SBM in the diets of weanling (75 g) Sprague-Dawley rats. Rats fed SBM had significantly (P < 0.05) higher average daily gain (ADG) than those fed Tower or Candle RSM, or diets containing the rapeseed meats. There was no significant (P < 0.05) difference in ADG, feed intake or feed to gain ratio of rats fed either Tower or Candle RSM. Feed intake, feed to gain ratio and fecal volatile fatty acid concentrations increased while average daily gain decreased with increasing level of hulls in simulated RSM diets. There was no significant difference (P < 0.05) in thyroid weight between rats fed SBM, Tower RSM or Candle RSM.

scholarly journals Erythropoietin Produces a Dual Effect on Carotid Body Chemoreception in Male Rats

2021 ◽  
Vol 12 ◽  
Author(s):  
Christian Arias-Reyes ◽  
Sofien Laouafa ◽  
Natalia Zubieta-DeUrioste ◽  
Vincent Joseph ◽  
Aida Bairam ◽  
...  
Keyword(s):  
Carotid Body ◽  
No Synthase ◽  
Male Rats ◽  
High Dose ◽  
No Production ◽  
Sprague Dawley ◽  
En Bloc ◽  
No Synthase Inhibitor ◽  
Sprague Dawley Rats ◽  
Synthase Inhibitor

Erythropoietin (EPO) regulates respiration under conditions of normoxia and hypoxia through interaction with the respiratory centers of the brainstem. Here we investigate the dose-dependent impact of EPO in the CB response to hypoxia and hypercapnia. We show, in isolated “en bloc” carotid body (CB) preparations containing the carotid sinus nerve (CSN) from adult male Sprague Dawley rats, that EPO acts as a stimulator of CSN activity in response to hypoxia at concentrations below 0.5 IU/ml. Under hypercapnic conditions, EPO did not influence the CSN response. EPO concentrations above 0.5 IU/ml decreased the response of the CSN to both hypoxia and hypercapnia, reaching complete inhibition at 2 IU/ml. The inhibitory action of high-dose EPO on the CSN activity might result from an increase in nitric oxide (NO) production. Accordingly, CB preparations were incubated with 2 IU/ml EPO and the unspecific NO synthase inhibitor (L-NAME), or the neuronal-specific NO synthase inhibitor (7NI). Both NO inhibitors fully restored the CSN activity in response to hypoxia and hypercapnia in presence of EPO. Our results show that EPO activates the CB response to hypoxia when its concentration does not exceed the threshold at which NO inhibitors masks EPO’s action.

scholarly journals Oral administration of Lactobacillus casei Shirota can ameliorate the adverse effect of an acute aflatoxin exposure in Sprague Dawley rats

2018 ◽  
Vol 88 (3-4) ◽  
pp. 199-208
Author(s):  
Elham Nikbakht ◽  
Rosita Jamaluddin ◽  
S. Mohd Redzwan ◽  
Saman Khalesi
Keyword(s):  
Adverse Effect ◽  
Lactobacillus Casei ◽  
Health Effect ◽  
Acute Exposure ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Lactobacillus Casei Shirota ◽  
Probiotic Treatment ◽  
Significant Difference ◽  
Aflatoxin B

Abstract. Aflatoxin B1 (AFB1) is a toxic compound commonly found in some crops with an adverse health effect on human and animals. Some beneficial microorganisms (or probiotics) such as lactic acid bacteria have shown the ability to reduce the bioavailability of aflatoxins and its intestinal absorption. However, the dose and duration of aflatoxins exposure and probiotic treatment can influence the ability of probiotics to remove aflatoxins. Therefore, this research aimed to investigate the efficacy of oral probiotic Lactobacillus casei Shirota strain (LcS) induction in an acute exposure to AFB1 in rats. Experimentally, Sprague Dawley rats were divided into three groups: AFB1 only (n = 9); AFB1 treated with LcS (n = 9); and control (no AFB1 exposure) (n = 6) groups. The blood AFB1 level of rats treated with LcS was slightly lower than the untreated AFB1 induced rats (11.12 ± 0.71 vs 10.93 ± 0.69 ng g–1). Also, LcS treatment slightly moderated the liver and kidney biomarkers in AFB1 induced rats. However, a trend for a significant difference was only observed in ALT of AFB1 induced rats treated with LcS compared to their counterparts (126.11 ± 36.90 vs 157.36 ± 15.46, p = 0.06). Rats’ body weight decreased in all animals force-fed with AFB1 with no significant difference between LcS treatment compared to the counterpart. In conclusion, this experiment indicated that probiotic LsC was able to slightly ameliorate the adverse effect of an acute exposure to AFB1 in rats. However, future studies with longer probiotics treatment or higher probiotics dose is required to confirm these findings.

scholarly journals Normogonadotropic hypogonadism in men with obesity

2009 ◽  
Vol 6 (3) ◽  
pp. 39-42 ◽  
Author(s):  
L V Savel'eva ◽  
R V Rozhivanov ◽  
B O Shurdumova ◽  
V V Fadeev
Keyword(s):  
Testosterone Level ◽  
Healthy Subjects ◽  
Immunoreactive Insulin ◽  
Homa Index ◽  
Obese Patients ◽  
Functional Disorder ◽  
Testosterone Levels ◽  
Significant Difference ◽  
Low Testosterone ◽  
Index Calculation

Objective: to evaluate the preavalence and characteristics of hypogonadism in obese man. Research and Methods: Thestudy was performed in 31 obese man (aged 33 [22;44], BMI 40 [34,4;44,0]. Measurements during the study: chemistry panel, testosteroa and LH levels, immunoreactive insulin, HOMA index calculation. Statistically significant difference w as considered as p < 0,05. Results. Hypogonadism preavalence was 80,6%. The hypogonadism prevalence and testosterone level was more depended on age and obesity but less on duration. 100 % risk of hypogonadism w as observed in heavily obese patients. There w as no increase of LH levels. In man with low testosterone lev els HOMA index w as significantly higher со mpared to healthy subjects. Conclusion: The prevalence of normogonadotropic hypogonadism in obese men is extremely high and it represents an evidence of functional disorder of hypophyseal gonadal system. Thedecrease of testosterone levels is age-coinciding, but it is more pronounced in obese nan.

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