scholarly journals The role of Helianthus tuberosus powder in healing of full-thickness wounds in mice

2021 ◽  
pp. 1290-1298
Author(s):  
Ali Ghazi Atiyah ◽  
Nadia Hameed Rija AL-Falahi
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Treated Group ◽  
Wound Contraction ◽  
Diffusion Method ◽  
Control Group ◽  
Full Thickness ◽  
Active Ingredients ◽  
Study Results ◽  
Thoracolumbar Region

Background and Aim: Recently, many medicinal plants have received considerable attention in the medical field because of their role in the wound healing potential. This study aimed to determine the effectiveness of H. tuberosus powder on the healing pathway of full-thickness cutaneous wounds in a mouse model. Materials and Methods: H. tuberosus powder was prepared by a freeze-drying process using a lyophilizer and its active ingredients were evaluated by high-performance liquid chromatography (HPLC), while its antibacterial properties were evaluated by agar well diffusion assay. The percentage wound contraction was also assessed. Thirty mice were used, which were divided equally into two groups, a control group and a treated group. A full-thickness wound, 1 cm×1 cm in size, was established on the dorsal aspect of the thoracolumbar region, into which H. tuberosus powder was topically applied in the treated group. In contrast, the control group was left without any treatment. The animals were euthanized on days 7, 14, and 21 after wounding for histopathological study. Results: The agar well diffusion method indicated the antibacterial activity of H. tuberosus, while the HPLC results indicated that the active ingredients of H. tuberosus powder mainly consisted of three major kinds of fatty acid. In addition, the macroscopic results of wound contraction rate and the histopathological outcomes of the healing process were significantly (p≤0.05) enhanced in the treated group compared with those in the control group. Conclusion: H. tuberosus powder acts as an antibacterial agent with the ability to enhance the wound healing process.

scholarly journals Silk derived formulations for accelerated wound healing in diabetic mice

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10232
Author(s):  
Muniba Tariq ◽  
Hafiz Muhammad Tahir ◽  
Samima Asad Butt ◽  
Shaukat Ali ◽  
Asma Bashir Ahmad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Chronic Wounds ◽  
Histological Analysis ◽  
Healing Process ◽  
Aloe Vera ◽  
Wound Contraction ◽  
Wound Dressings ◽  
Control Group ◽  
Diabetic Mice

Background The present study aimed to prepare effective silk derived formulations in combination with plant extract (Aloe vera gel) to speed up the wound healing process in diabetic mice. Methods Diabetes was induced in albino mice by using alloxan monohydrate. After successful induction of diabetes in mice, excision wounds were created via biopsy puncture (6 mm). Wound healing effect of silk sericin (5%) and silk fibroin (5%) individually and in combination with 5% Aloe vera gel was evaluated by determining the percent wound contraction, healing time and histological analysis. Results The results indicated that the best biocompatible silk combination was of 5% silk fibroin and 5% Aloe vera gel in which wounds were healed in 13 days with wound contraction: 98.33 ± 0.80%. In contrast, the wound of the control group (polyfax) healed in 19 day shaving 98.5 ± 0.67% contraction. Histological analysis revealed that the wounds which were treated with silk formulations exhibited an increased growth of blood vessels, collagen fibers, and much reduced inflammation. Conclusion It can be concluded that a combination of Bombyx mori silk and Aloe vera gel is a natural biomaterial that can be utilized in wound dressings and to prepare more innovative silk based formulations for speedy recovery of chronic wounds.

scholarly journals EFEK SERBUK KOPI ROBUSTA (Coffea canephora) TERHADAP PENYEMBUHAN LUKA INSISI PADA KELINCI (Oryctolagus cuniculus)

2015 ◽  
Vol 3 (3) ◽  
Author(s):  
Lilian N. Artho ◽  
Jane Wuisan ◽  
J. A. Najoan
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Treated Group ◽  
Coffea Canephora ◽  
Control Group ◽  
Wound Healing Process ◽  
Robusta Coffee ◽  
Coffee Powder ◽  
The Right ◽  
Sterile Gauze

Abstract: Coffee is known as food and drink as well as alternative medicine for various types of wounds. One of the most coffee plants in Indonesia is Robusta coffee (Coffea canephora). This study aimed to determine whether Robusta coffee powder could accelerate the wound healing process. This was an experimental study using four rabbits as subjects. Incisions were made on the rabbit backs, right and left, with a length of 5 cm. Wounds on the left backs, the untreated/control group, were only covered with sterile gauze. Wounds on the right backs, the treated group, were applied with robusta coffee powder and covered with sterile gauze. Robusta coffee powder and gauze were replaced two times a day. Wounds were macroscopically observed for 2 weeks. The results showed that at day 7, the wounds were still equally opened, dry, looked not too deep, and there was a crust on each wound surface. The lengths of the treated wounds were shorter than of the control wounds. At day 14, the edges of the treated wounds looked closer, the crusts were still attached to the wound surfaces and were smaller than of the control wounds. Conclusion: Robusta coffee powder (Coffea canephora) could accelerate the healing process of incised wounds on rabbit skin.Keywords: wound healing, wound incision, robusta coffee powder, coffea canephoraAbstrak: Kopi bukan hanya dikenal sebagai makanan dan minuman saja tetapi bisa juga sebagai obat alternatif dalam menangani berbagai jenis luka. Salah satu jenis tanaman kopi yang paling banyak di Indonesia ialah kopi Robusta (Coffea canephora). Penelitian ini bertujuan untuk menentukan apakah sebuk kopi robusta mempunyai efek untuk mempercepat proses penyembuhan luka. Penelitian ini menggunakan metode eksperimental dengan 4 ekor kelinci sebagai hewan coba. Luka insisi dibuat pada punggung kanan dan kiri kelinci dengan panjang 5 cm. Luka pada punggung kiri merupakan luka kontrol yang tidak diberi perlakuan, hanya ditutupi dengan kasa steril. Luka pada punggung kanan merupakan luka perlakuan dengan diberi serbuk kopi robusta lalu ditutup kasa steril. Serbuk kopi robusta dan kasa diganti dua kali sehari. Pengamatan dilakukan selama 2 minggu dengan membandingkan gambaran makroskopik kedua luka. Hasil penelitian memperlihatkan pada hari ke 7 kedua luka masih sama-sama terbuka, kering, tampak sudah tidak terlalu dalam, dan terdapat kerak yang menempel pada permukaan luka. Ukuran luka perlakuan lebih pendek dari luka kontrol. Hari ke 14 kedua luka sudah mulai menutup, kerak masih menempel pada permukaan luka dan ukuran kerak luka perlakuan lebih kecil dari luka kontrol. Simpulan: Serbuk kopi robusta (Coffea canephora) memiliki efek untuk mempercepat penyembuhan luka insisi pada kulit kelinci.Kata kunci: penyembuhan luka, luka insisi, kopi robusta, coffea canephora

Accelerated wound healing process in rat by probiotic Lactobacillus reuteri derived ointment

2019 ◽  
Vol 30 (3) ◽  
Author(s):  
Zohreh Khodaii ◽  
Saman Afrasiabi ◽  
Seied Ali Hashemi ◽  
Abdolreza Ardeshirylajimi ◽  
Mahboobeh Mehrabani Natanzi
Keyword(s):  
Wound Healing ◽  
Lactobacillus Reuteri ◽  
Histopathological Examination ◽  
Healing Process ◽  
Treated Group ◽  
Probiotic Bacterium ◽  
Control Group ◽  
Wound Healing Process ◽  
Positive Effects ◽  
Antioxidant Agents

Abstract Background Antioxidant agents have positive effects on wound healing process and on the other hand probiotics also have great antioxidation or oxidation-resistance properties. Methods In this study, we investigated the effects of Lactobacillus reuteri derived ointment as a probiotic bacterium on the wound healing process using a full-thickness rat skin model. The wound diameter and contraction percent measurement, histopathological examination, and myeloperoxidase (MPO) enzyme activity evaluation were carried out during the period of study. Results Amount of deposit collagen was significantly increased; epithelization speed and epidermis production were also observed in the ointment-treated group, while the inflammation rate was also significantly lower than the control group. In addition, a significant reduction in the activity of enzyme MPO was also detected in the ointment-treated group. Conclusions According to the results, probiotic ointment effectively accelerates the wound healing process and it can be used as a promising drug for wound dressing application.

The effect of Silver Nanoparticle Induced Diabetic on Wound Healing Full Thickness Pseudomonas aeruginosa Contaminated Mouse Skin Wound Models

2019 ◽  
Vol 10 (04) ◽  
pp. 720-724
Author(s):  
Thamer Mutlag Jasim ◽  
Abas Adel Latif
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Wound Healing ◽  
Silver Nanoparticle ◽  
Mouse Skin ◽  
Treated Group ◽  
Skin Wound ◽  
Control Group ◽  
Diabetic Wound ◽  
Ag Nps ◽  
Study Results

This research was considered to evaluate the antidiabetic effect of silver nanoparticle (AgNps)and following experimental diabetic. In the present study. Thirty healthy swiss mice aged between 7-8 weeks, old male mice and divided into six groups of five animals. Diabetic induced mice by using intraperitoneal (IP) injection of alloxan (180 mg lkg). Group 1 included non diabetic control, Group 2 Diabetic, Group 3 Diabetic +0.01 mg AgNps Group 4 Diabetic +0.05 AgNps, Group 5 Diabetic+ wound contaminated with Pseudomonas aeruginosa. Group 6 ( diabetic +contaminated wound + silver nanoparticle (Ag Nps). Silver nanoparticle show ample antibacterial activities. The result of the current study introduced an invivo silvernanoparticle accelerate by effects on the treatment of Pseudomonas aeruginosa infected skin wound. The present study was conducted to synthesis the AgNps biologically and evaluate its antibacterial activity against Pseudomonas aeruginosa diabetes induced by Alloxan in mice. Adminstration of silver nanoparticle resulted in significance antidiabetic effects that is is improved glucose tolerance higher source. The current study results are presented for the first time which suggest for the development of AgNps as anantdiabetic factor in future. The broad spectrum of bioactivity of AgNPs makes them promising agent not only to fight infection, but to sterile the wound and accelerate wound healing. There were significant higher wound healing scores in Nanoparticle treated group. Compared with control group. These result suggest that nanoparticle may be useful in diabetic wound healing. Treatment with asingle dose of AGNPs produced amild reduction in blood glucose and some reduction in plasma insulin at 2 h.The present results revealed the potential of the synthesized Ag-NPs as safer bactericidal agents for the treatment of diabetes induced wound contaminated with P.aeruginosa.

scholarly journals TopicalHypericum perforatumImproves Tissue Regeneration in Full-Thickness Excisional Wounds in Diabetic Rat Model

2015 ◽  
Vol 2015 ◽  
pp. 1-4 ◽  
Author(s):  
Soheila Yadollah-Damavandi ◽  
Mehdi Chavoshi-Nejad ◽  
Ehsan Jangholi ◽  
Noushin Nekouyian ◽  
Sahar Hosseini ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Healing Process ◽  
Treated Group ◽  
Volume Density ◽  
Diabetic Rat ◽  
Control Group ◽  
Wound Healing Process ◽  
Numerical Density ◽  
Full Thickness ◽  
Closure Rate

Delayed wound healing process is one of the most important concerns in diabetes. Healing of wounds has four phases, namely, hemostasis, inflammation, proliferation, and remodeling. For a successful repair, all four factors must occur properly. Hence, we aimed to evaluate the healing effects ofHypericum perforatum(HP) on full-thickness diabetic skin wounds by using stereological methods. Forty-eight female diabetic rats were randomly divided into four groups (n=12): gel base treated group, HP 5% gel treated group, HP 10% gel treated group, and the control group which received no treatment. A circular 1 cm2full-thickness wound was created on the animal’s neck and wound area was measured every three days. After sacrificing the animals, skin samples were fixed and prepared for stereological evaluations. Based on the results, HP treated group showed faster wound closure rate in comparison with control and vehicle groups (P<0.05). In addition, numerical density of fibroblasts, volume density of collagen bundles, and mean diameter and volume densities of the vessels in HP group were significantly higher than control and vehicle groups. The results of this study showed that HP has the ability to improve tissue regeneration by enhancing fibroblast proliferation, collagen bundle synthesis, and revascularization.

scholarly journals THE DYNAMICS OF WOUND HEALING IN THE TREATMENT WITH THE OINTMENT WITH A DRY EXTRACT OF POTENTILLA

2018 ◽  
Vol 25 (6) ◽  
pp. 105-109
Author(s):  
L. P. Lezhnevа ◽  
A. M. Temirbulatova ◽  
E. F. Stepanova ◽  
D. V. Veselova
Keyword(s):  
Wound Healing ◽  
Dosage Form ◽  
Healing Process ◽  
Control Group ◽  
Wound Healing Process ◽  
Skin Wounds ◽  
Full Thickness ◽  
Dry Extract ◽  
Full Thickness Skin ◽  
Thickness Skin

Aim. The study was designed for the evaluation of the effect of ointments with dry extracts of potentilla on the dynamics of the healing process of full-thickness skin wounds.Materials and methods. The wound healing activity estimation of the proposed dosage form was conducted on white Wistar rats. The results of macroscopic studies (the area of the wound defect, the state of the scab, the presence of hemorrhage beneath it, the degree of inflammatory processes in the underlying tissues, including deeply located tissue structures, up to the muscle elements) and the results of histological examination were the criterion for evaluating the effectiveness of the wound healing agent. In the course of the experiment, the animals were divided into 4 groups: the main group (treatment with the ointment with a dry extract of potentilla), control group No. 1 (treatment with the ointment base), control group No. 2 (without treatment), and control group No. 3 (treatment with the «Chlorophyllipt» drug.Results. The results of the macroscopic and histological studies made it possible to conclude that the wound healing process in the treatment with the ointment with a dry extract of potentilla had a number of features. The proposed dosage form enhanced the regeneration of the multilayered squamous epithelium, accelerated the maturation of the granulation tissue, and eliminated the inflammation and infiltration in the first days of treatment, which led to a significant reduction in the healing time when it was applied (15-18 days). In the animals of the control groups No. 1 and No. 2, the described processes occurred much more slowly, and inflammatory infiltration persisted almost throughout the entire experiment. The wound surface was healed on the 27-30th day. A lot of common features were noted in the mechanism and timing of wound healing in the treatment with the ointment with a dry extract of potentilla and a reference preparation – «Сhlorophyllipt». Conclusion. The ointment with a dry extract of potentilla significantly speeds up the healing process of full-thickness skin wounds.

scholarly journals Healing Effect of Sesame Ointment on Second-degree Burn Wound in Rats

2016 ◽  
Vol 5 (2) ◽  
pp. 56-62
Author(s):  
Setareh Tehrani ◽  
Parisa Lotfi ◽  
Sepideh Tehrani ◽  
Ehsan Jangholi ◽  
Hoda Aryan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Treated Group ◽  
Sesame Oil ◽  
Control Group ◽  
Burn Wound ◽  
Matrix Deposition ◽  
Degree Burn ◽  
Anti Inflammatory ◽  
Second Degree

Background: Wound healing requires processes like cell migration, proliferation of extracellular matrix deposition, remodeling and angiogenesis. Also, anti-oxidative and anti-inflammatory reactions play key roles in the progression of wound healing. Previous studies showed that sesame oil has anti-inflammatory, antimicrobial and antioxidant activities. In addition, it is shown that sesame oil stimulates fibroblast production in vitro and in skin wounds. Therefore, in this study we investigated the effects of sesame ointment on the process of second-degree burn wound healing in rat models according to stereological parameters. Materials and Methods: In this experimental study, forty male Wistar rats (200±20 g) were randomly divided into four groups (n=10): ointment-base treated group (vehicle), silver sulfadiazine (SDD) treated group, sesame treated group (E1) and the control group which received no treatment. A 2×3 cm2 standard second-degree burn wound was induced on the posterior surface of animal’s neck under general anesthesia. After sacrifice, the animal’s skin sample was fixed in buffered formaldehyde for stereological evaluations. The data was analyzed by SPSS statistical software (version 14.0). P<0.05 was considered statistically significant. Results: According to our results, the mean of reduction in wound areas, volume density of collagen bundles and hair folicules, fibroblast populations, length density of vessels in E1 group was significantly higher than control group (P<0.05). The differences between E1 and SSD were not statistically noticeable regarding the stereological parameters. Conclusion: According to stereological analysis, administration of sesame ointment showed the ability to improve wound healing process and tissue regeneration in the treatment of skin damages.[GMJ.2016;5(2):56-62]

Ethanol Extract of Achillea fragrantissima Enhances Angiogenesis through Stimulation of VEGF Production

2020 ◽  
Vol 21 ◽  
Author(s):  
Hana M. Hammad ◽  
Amer Imraish ◽  
Maysa Al-Hussaini ◽  
Malek Zihlif ◽  
Amani A. Harb ◽  
...  
Keyword(s):  
Wound Healing ◽  
Rural Communities ◽  
Ex Vivo ◽  
Ethanol Extract ◽  
Aortic Ring ◽  
Treated Group ◽  
Control Group ◽  
Dependent Manner ◽  
Achillea Fragrantissima

Objective: Achillea fragrantissima L. (Asteraceae) is a traditionally used medicinal herb in the rural communities of Jordan. Methods: The present study evaluated the efficacy of the ethanol extract of this species on angiogenesis in both, ex vivo using rat aortic ring assay and in vivo using rat excision wound model. Results: In concentrations of 50 and 100 µg/ml, the ethanol extract showed angiogenic stimulatory effect and significantly increased length of capillary protrusions around aorta rings of about 60% in comparison to those of untreated aorta rings. In MCF-7 cells, the ethanol extract of A. fragrantissima stimulates the production of VEGF in a dose-dependent manner. 1% and 5% of ethanol extract of A. fragrantissima containing vaseline based ointment was applied on rat excision wounds for six days and was found to be effective in wound healing and maturation of the scar. Both preparations resulted in better wound healing when compared to the untreated control group and vaseline-treated group. This effect was comparable to that induced by MEBO, the positive control. Conclusion: The results indicate that A. fragrantissima has a pro-angiogenic effect, which may act through the VEGF signaling pathway.

Cold Plasma Treatment Promotes Full-thickness Healing of Skin Wounds in Murine Models

2021 ◽  
pp. 153473462110021
Author(s):  
Joon M. Jung ◽  
Hae K. Yoon ◽  
Chang J. Jung ◽  
Soo Y. Jo ◽  
Sang G. Hwang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Plasma Treatment ◽  
Cold Plasma ◽  
Smooth Muscle Actin ◽  
Treated Group ◽  
Control Group ◽  
Alpha Smooth Muscle Actin ◽  
Skin Wound Healing

Cold plasma can be beneficial for promoting skin wound healing and has a high potential of being effectively used in treating various wounds. Our aim was to verify the effect of cold plasma in accelerating wound healing and investigate its underlying mechanism in vitro and in vivo. For the in vivo experiments, 2 full-thickness dermal wounds were created in each mouse (n = 30). While one wound was exposed to 2 daily plasma treatments for 3 min, the other wound served as a control. The wounds were evaluated by imaging and histological analyses at 4, 7, and 11 days post the wound infliction process. Immunohistochemical studies were also performed at the same time points. In vitro proliferation and scratch assay using HaCaT keratinocytes and fibroblasts were performed. The expression levels of wound healing–related genes were analyzed by real-time polymerase chain reaction and western blot analysis. On day 7, the wound healing rates were 53.94% and 63.58% for the control group and the plasma-treated group, respectively. On day 11, these rates were 76.05% and 93.44% for the control and plasma-treated groups, respectively, and the difference between them was significant ( P = .039). Histological analysis demonstrated that plasma treatment promotes the formation of epidermal keratin and granular layers. Immunohistochemical studies also revealed that collagen 1, collagen 3, and alpha-smooth muscle actin appeared more abundantly in the plasma-treated group than in the control group. In vitro, the proliferation of keratinocytes was promoted by plasma exposure. Scratch assay showed that fibroblast exposure to plasma increased their migration. The expression levels of collagen 1, collagen 3, and alpha-smooth muscle actin were elevated upon plasma treatment. In conclusion, cold plasma can accelerate skin wound healing and is well tolerated.

scholarly journals In vivo evaluation of a recombinant N-acylhomoserine lactonase formulated in a hydrogel using a murine model infected with MDR Pseudomonas aeruginosa clinical isolate, CCASUP2

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Masarra M. Sakr ◽  
Walid F. Elkhatib ◽  
Khaled M. Aboshanab ◽  
Eman M. Mantawy ◽  
Mahmoud A. Yassien ◽  
...  
Keyword(s):  
Survival Rate ◽  
Murine Model ◽  
Histopathological Examination ◽  
Healing Process ◽  
Bacterial Count ◽  
Treated Group ◽  
Control Group ◽  
In Vivo Evaluation ◽  
Systemic Spread

AbstractFailure in the treatment of P. aeruginosa, due to its broad spectrum of resistance, has been associated with increased patient mortality. One alternative approach for infection control is quorum quenching which was found to decrease virulence of such pathogen. In this study, the efficiency of a recombinant Ahl-1 lactonase formulated as a hydrogel was investigated to control the infection of multidrug resistant (MDR) P. aeruginosa infected burn using a murine model. The recombinant N-acylhomoserine lactonase (Ahl-1) was formulated as a hydrogel. To test its ability to control the infection of MDR P. aeruginosa, a thermal injury model was used. Survival rate, and systemic spread of the infection were evaluated. Histopathological examination of the animal dorsal skin was also done for monitoring the healing and cellular changes at the site of infection. Survival rate in the treated group was 100% relative to 40% in the control group. A decrease of up to 3 logs of bacterial count in the blood samples of the treated animals relative to the control group and a decrease of up to 4 logs and 2.3 logs of bacteria in lung and liver samples, respectively were observed. Histopathological examination revealed more enhanced healing process in the treated group. Accordingly, by promoting healing of infected MDR P. aeruginosa burn and by reducing systemic spread of the infection as well as decreasing mortality rate, Ahl-1 hydrogel application is a promising strategy that can be used to combat and control P. aeruginosa burn infections.

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