Biochemical and histopathological changes related to the topical application of Aloe vera ointment for canine pyoderma

Background and Aim: Pyoderma is common in dogs, and its treatment requires a novel medication rather than antibiotic therapy. This study aimed to determine the biochemical and histopathological changes associated with the topical application of Aloe vera 20% and 40% ointments, compared with gentamicin 0.1% ointment, in dogs suffering from Staphylococcus aureus pyoderma. Materials and Methods: Serum and skin samples were collected from a negative control group before inducing pyoderma and from other subdivided groups on the 3rd, 7th, 10th, and 14th days post-inoculation for biochemical and histopathology examination. Results: Serum aspartate aminotransferase, alanine aminotransferase (ALT), urea, and creatinine concentrations were higher in the positive control dogs on the 3rd day without treatment (DWT) compared with the negative control dogs (p<0.05). Compared with the healthy control dogs, serum zinc concentrations were lower in the positive control group on the 3rd, 7th, and 10th DWT and in dogs treated with A. vera 20% and gentamicin 0.1% ointments on the 3rd and 7th days post-treatment (p<0.05). Grossly, skin had erythema, pruritus, and pus-filled pustules of the untreated group. Microscopically, skin showed epidermal necrosis and edema, dermal collagen necrosis, and severe neutrophilic infiltration. Conclusion: Compared with A. vera 20% and gentamicin 0.1% ointments, the topical application of A. vera 40% ointmentinduced quicker skin healing and decreased the inflammatory changes caused by S. aureus inoculation, based on biochemical and histopathological changes reflective of its curative efficiency. A. vera 40% ointment may be a suitable alternative to antibiotics for the treatment of staphylococcal pyoderma in dogs.

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ALOE VERA ETHANOL EXTRACT AS A THERAPY FOR ALVEOLAR MANDIBLE REGENERATION IN LIPOPOLYSACCHARIDE-EXPOSED RATS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018.v10s1.03 ◽

2018 ◽

Vol 10 (1) ◽

pp. 11

Author(s):

Latifah Fitriani Rakhman ◽

Octaviana Widya Pangestika ◽

Delvi Fitriani ◽

Rudhanton Rudhanton ◽

Nur Permatasari

Keyword(s):

Alveolar Bone ◽

Aloe Vera ◽

Ethanol Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Alveolar Tissue ◽

Hematoxylin Eosin ◽

One Way Anova

Objective: The study examined the effects of Aloe vera ethanol extract on alveolar mandible regeneration in rats (Rattus norvegicus).Methods: Rats were divided into five groups as follows: Negative control group received no treatment, positive control group received Escherichiacoli lipopolysaccharide (LPS) alone for 5 days, and Groups P1, P2, and P3 received LPS for 5 days followed by 40, 80, and 160 mg/kg BW A. veraethanol extract, respectively, orally on days 6–12. Alveolar bone tissues were taken and histologically processed through hematoxylin-eosin staining.Osteoblast and osteoclast numbers in alveolar tissue were also examined.Results: One-way ANOVA revealed strong relationships between the A. vera ethanol extract dosage and the numbers of osteoblasts (r=0.921, p<0.05)and osteoclasts (r=−0.631, p<0.05) in rats.Conclusion: A. vera ethanol extract appears capable of stimulating alveolar bone regeneration following LPS exposure.

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EFFICACY OF A STANDARDIZED ETHANOL EXTRACT OF ROSELLE CALYX IN THE TREATMENT OF ORAL MUCOSA ULCERATION (IN VIVO)

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10s5.23127 ◽

2017 ◽

Vol 10 (17) ◽

pp. 183

Author(s):

Reni Jayantini ◽

Dewi Fatma Suniarti ◽

Agoeng T Sarwono

Keyword(s):

Topical Application ◽

Ethanol Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Sprague Dawley ◽

Ethanol Extracts ◽

Inflammation Score

Objectives: To analyze the effectiveness of the topical application of a standardized ethanol extract of roselle calyx in the treatment of oral mucous ulceration.Methods: Twelve Sprague Dawley rats were divided into two groups, with oral mucous ulcer being induced in all the rats. The ulcer diameter, reddish color, and the swollen membrane around the ulcer, as well as the inflammation score of the ulcer, were recorded for all animals. The negative control group was treated with aquades, while the positive control group was treated with 0.1% triamcinolone acetonide. The treatment group received the topical application of 7.5% and 15% standardized ethanol extract of roselle calyx twice a day for 3 or 7 days. On the 4th day, six rats were sacrificed, while the remaining six rats continued to receive treatment as before. On the 8th day, all the rats were sacrificed. Specimens were fixed, and histology slides were made. Further, microscopic slides were observed and scored.Result: Both the 7.5% and 15% standardized ethanol extracts of roselle calyx were found to reduce the ulcer diameter and inflammation score. No swelling or redness was observed.Conclusions: The application of 7.5% standardized ethanol extract of roselle calyx for 7 days is equally as effective as the application of 0.1% triamcinolone acetonides, except in terms of the decrease in the inflammation score.

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The Effect of Herbal Infusion on Fasting Blood Glucose (FBS) and Malondyaldehyde (MDA) Levels in Diabetic Rats

Materials Science Forum ◽

10.4028/www.scientific.net/msf.1009.9 ◽

2020 ◽

Vol 1009 ◽

pp. 9-14

Author(s):

Apriliani Ismi Fauziah ◽

Lintang Dian Saraswati ◽

Ari Udiyono ◽

Beti Safitri

Keyword(s):

Blood Glucose ◽

Fasting Blood Glucose ◽

Aloe Vera ◽

Control Sample ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Diabetic Rats ◽

Control Group ◽

Albino Rats

Aloe vera, ginger, and sappan wood have recently gained attention as a potent antioxidant. This study was undertaken to analyze the possible effects of oral administration of herbal infusion (the combination of Aloe vera, ginger, and sapang wood) on fasting blood glucose (FBS) and malondialdehyde (MDA) levels in diabetic rats. Forty male albino rats (150-250 gm) were divided into five groups (n=8 in each group): alloxan-induced diabetic (negative control group/K-), acarbose treated (positive control group/K+), diabetic rats treated with herbal infusion dose 6.75 mg/150 gmbw (P1), diabetic rats treated with herbal infusion dose 13.5 mg/150 gmbw (P2), and diabetic rats treated with herbal infusion dose 20.25 mg/150 gmbw (P3). Male albino rats induced with a dose of alloxan 120 mg/kgbw and also 5% glucose after 6 hours of alloxan induction. FBS and MDA levels of each rat were measured before induction of alloxan was performed as a control sample. After seven days and 14 days of herbal infusion administration, FBS parameters was investigated with a glucometer. In the last week, the concentration of MDA in serum blood was determined using a UV-VIS spectrophotometer with a wavelength of 545 nm. Diabetic rats exhibited a significant decrease in FBS and MDA. Administration of herbal infusion was reduced FBS significantly between groups (ANOVA, Bonferroni, p<0.05). The concentration of MDA in serum blood was decreased significantly on K-, K+, P1, P2, P3 compared to the control group (ANOVA, LSD, P<0.05). It could be postulated herbal infusion has anti-hyperglycemic and anti-diabetic effects of FBS and MDA in male albino rats Wistar strain.

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The Comparison of Ostecyte in the Pressure area and Tension area on Tooth Movement Because of Hyperbaric Oxygen Therapy

DENTA ◽

10.30649/denta.v12i1.162 ◽

2018 ◽

Vol 12 (1) ◽

pp. 34

Author(s):

Arya Barahmanta ◽

Muhammad Faizal Winaris ◽

Pambudi Raharjo

Keyword(s):

Hyperbaric Oxygen ◽

Oxygen Therapy ◽

Tooth Movement ◽

Bone Remodelling ◽

Orthodontic Tooth Movement ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Pressure Area

Background: Orthodontic tooth movement is a interaction prosess of resorption and deposition of bone remodeling. Orthodontic tooth movement by mechanical strength causes changes in alveolar bone. Osteocyte is an essential cell to respond bone remodelling. Hyperbaric Oxygen Therapy affects production of osteocyte because it can release Reactive Oxygen Species (ROS) and Nitrid Oxide (NO). Purpose: To determine the difference number of osteocyte in pressure and tension area during tooth movement by adjuvant of Hyperbaric Oxygen 2,4 ATA during 7 days starting on day 8 to day 14. Materials and Methods: This research used Completery Randomized Control Group Post Test Only Design. 36 cavia cobaya (male) were divided into 3 groups randomly : the negative control groups, positive control group, and treatment group. Preparat staining used Hematoxylin Eosin (HE) and calculated on microscop 1000x with 20 field of view. Data analyses used one way ANOVA and LSD test then compared each area by using paired T test. Result: The data showed that the treatment group (P=10,67) tension area has the highest number of osteocyte than negative control group (K-=3,67), positive control (K+=7,42). In the pressure area showed that negative control group (K-=5,00) has the highest than positive control group (K+=3,83) and treatment (P=3,25). Conclusion: Therapy HBO 2,4 ATA 7 days starting on day 8 to day 14 is could increase osteocyte in the tissue to stimulate process of bone remodelling.<pre> </pre>Keywords: Hyperbaric Oxygen, Tooth movement, Bone remodeling, Osteocyte Correspondence: Arya Brahmanta, Department of Orthodonty, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email: <a href="mailto:[email protected]">arya.brahmanta@hangtuah.ac.id</a>

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Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

Jurnal Katalisator ◽

10.22216/jk.v3i1.2178 ◽

2018 ◽

Vol 3 (1) ◽

pp. 1

Author(s):

Verawaty Verawaty ◽

Dhea Claudia Novel

Keyword(s):

Blood Glucose ◽

Ethanol Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Male Mice ◽

Glucose Levels ◽

The Third ◽

Blood Glucose Levels

Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.

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Resistance to Fracture of Dental Roots Obturated with Different Materials

BioMed Research International ◽

10.1155/2015/591031 ◽

2015 ◽

Vol 2015 ◽

pp. 1-5 ◽

Cited By ~ 6

Author(s):

Berkan Celikten ◽

Ceren Feriha Uzuntas ◽

Kamran Gulsahi

Keyword(s):

Fracture Resistance ◽

Testing Machine ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Positive Control Group ◽

Control Group ◽

Root Canal Filling ◽

Filling Materials ◽

Group 2

The aim of this study was to compare the vertical fracture resistance of roots obturated with different root canal filling materials and sealers. Crowns of 55 extracted mandibular premolar teeth were removed to provide root lengths of 13 mm. Five roots were saved as negative control group (canals unprepared and unfilled). Fifty root canals were instrumented and then five roots were saved as positive control group (canals prepared but unfilled). The remaining 45 roots were randomly divided into three experimental groups (n=15root/group) and obturated with the following procedures: in group 1, glass ionomer-based sealer and cone (ActiV GP obturation system); in group 2, bioceramic sealer and cone (EndoSequence BC obturation system); and in group 3, roots were filled with bioceramic sealer and cone (Smartpaste bio obturation system). All specimens were tested in a universal testing machine for measuring fracture resistance. For each root, the force at the time of fracture was recorded in Newtons. The statistical analysis was performed by using Kruskal-Wallis and post hoc test. There were no significant differences between the three experimental groups. The fracture values of three experimental and negative control groups were significantly higher than the positive control group. Within the limitations of this study, all materials increased the fracture resistance of instrumented roots.

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Ranitidine as an alcohol dehydrogenase inhibitor in acute methanol toxicity in rats

Human & Experimental Toxicology ◽

10.1177/0960327109353777 ◽

2009 ◽

Vol 29 (2) ◽

pp. 93-101 ◽

Cited By ~ 8

Author(s):

Amal A El-Bakary ◽

Sahar A El-Dakrory ◽

Sohayla M Attalla ◽

Nawal A Hasanein ◽

Hala A Malek

Keyword(s):

Alcohol Dehydrogenase ◽

High Performance ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Sprague Dawley ◽

Blood Samples ◽

Methanol Poisoning ◽

Sprague Dawley Rats

Methanol poisoning is a hazardous intoxication characterized by visual impairment and formic acidemia. The therapy for methanol poisoning is alcohol dehydrogenase (ADH) inhibitors to prevent formate accumulation. Ranitidine has been considered to be an inhibitor of both gastric alcohol and hepatic aldehyde dehydrogenase enzymes. This study aimed at testing ranitidine as an antidote for methanol acute toxicity and comparing it with ethanol and 4-methyl pyrazole (4-MP). This study was conducted on 48 Sprague-Dawley rats, divided into 6 groups, with 8 rats in each group (one negative control group [C1], two positive control groups [C2, C3] and three test groups [1, 2 and 3]). C2, C3 and all test groups were exposed to nitrous oxide by inhalation, then, C3 group was given methanol (3 g/kg orally). The three test groups 1, 2 and 3 were given ethanol (0.5 g/kg orally), 4-MP (15 mg/kg intraperitoneally) and ranitidine (30 mg/kg intraperitoneally), respectively, 4 hours after giving methanol. Rats were sacrificed and heparinized, cardiac blood samples were collected for blood pH and bicarbonate. Non-heparinized blood samples were collected for formate levels by high performance liquid chromatography. Eye balls were enucleated for histological examination of the retina. Ranitidine corrected metabolic acidosis (p = .025), decreased formate levels (p = .014) and improved the histological findings in the retina induced by acute methanol toxicity.

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Effect of a Polyphenol-Rich Extract from Aloe vera Gel on Experimentally Induced Insulin Resistance in Mice

The American Journal of Chinese Medicine ◽

10.1142/s0192415x07005491 ◽

2007 ◽

Vol 35 (06) ◽

pp. 1037-1046 ◽

Cited By ~ 39

Author(s):

Yolanda Y. Pérez ◽

Enrique Jiménez-Ferrer ◽

Alejandro Zamilpa ◽

Marcelino Hernández-Valencia ◽

Francisco J. Alarcón-Aguilar ◽

...

Keyword(s):

Insulin Resistance ◽

Body Weight ◽

Aloe Vera ◽

Negative Control Group ◽

Negative Control ◽

Control Group ◽

Glucose Levels ◽

Insulin Tolerance ◽

Aloe Vera Gel ◽

Experimentally Induced

Insulin resistance, which precedes type 2 diabetes mellitus (T2DM), is a widespread pathology associated with the metabolic syndrome, myocardial ischemia, and hypertension. Finding an adequate treatment for this pathology is an important goal in medicine. The purpose of the present research was to investigate the effect of an extract from Aloe vera gel containing a high concentration of polyphenols on experimentally induced insulin resistance in mice. A polyphenol-rich Aloe vera extract (350 mg/kg) with known concentrations of aloin (181.7 mg/g) and aloe-emodin (3.6 mg/g) was administered orally for a period of 4 weeks to insulin resistant ICR mice. Pioglitazone (50 mg/kg) and bi-distilled water were used as positive and negative controls respectively. Body weight, food intake, and plasma concentrations of insulin and glucose were measured and insulin tolerance tests were performed. The insulin resistance value was calculated using the homeostasis model assessment for insulin resistance (HOMA-IR) formula. Results showed that the polyphenol-rich extract from Aloe vera was able to decrease significantly both body weight ( p < 0.008) and blood glucose levels ( p < 0.005) and to protect animals against unfavorable results on HOMA-IR, which was observed in the negative control group. The highest glucose levels during the insulin tolerance curve test were in the negative control group when compared to the Aloe vera extract and pioglitazone treated mice ( p < 0.05). In conclusion, Aloe vera gel could be effective for the control of insulin resistance.

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Effect of Chayote (Sechium Edule Jacq. Swartz) Extract on Level of Interleukin-8 in Wistar Rats with Aspirin-Induced Gastritis

Childhood Stunting, Wasting, and Obesity, as the Critical Global Health Issues: Forging Cross-Sectoral Solutions ◽

10.26911/the7thicph.05.35 ◽

2020 ◽

Author(s):

Hendrika Andriana Silitonga ◽

◽

Gontar Alamsyah Siregar ◽

Rosita Juwita Sembiring ◽

Marline Nainggolan ◽

...

Keyword(s):

Ethyl Acetate ◽

Wistar Rats ◽

Ethanol Extract ◽

Ethyl Acetate Fraction ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Interleukin 8 ◽

Control Group ◽

Sechium Edule

ABSTRACT Background: Recent studies showed that Interleukin-8 (IL-8), activated cytokine immune response which plays an important role in the development of acute and chronic gastritis. Harmless anti-inflammatory therapeutic alternatives have been proposed, for example, the consumption of Sechium Edule Jacq. Swartz (chayote). Antioxidant (flavonoid) and cell regeneration (alkaloid) agents were found in chayote. This study aimed to determine the effect of chayote Sechium Edule Jacq. Swartz extracts on the level of IL-8 in Wistar rats with aspirin- induced gastritis. Subjects and Method: This was a randomized controlled trial (RCT) conducted at the laboratory of Mathematics and Natural Science, Universitas Sumatra Utara from January to February 2020. A total of 35 male Wistar rats was selected for this study and randomly allocated into 7 groups: (1) Negative control; (2) Positive control; (3) 100 mg/ kg BW chayote ethanol extract ; (4) 200 mg/kg BW chayote ethanol extract; (5) 100 mg/ kg BW chayote ethyl acetate fraction; (6) 200 mg/kg BW chayote ethyl acetate fraction; and (7) 20 mg omeprazole. The rats in positive control and treatment groups were induced with aspirin (200mg/ kg BW). The negative control group received no intervention. The dependent variable was level of IL-8 measured by ELISA. The independent variables were treatment status. The data were analyzed by One Way Anova and post hoc test. Results: The mean differences of IL-8 level were not statistically significant between study groups (p= 0.327). Mean of IL-8 level was higher in positive control group (Mean= 160.80; SD= 6.90) than in negative control group (Mean= 141.20; SD= 10.98). The lowest IL-8 level was in 100mg/ kg BW chayote ethanol extract group (Mean= 149.94; SD= 40.4), followed by 200mg/ kg BW (Mean= 152.4; SD= 30.73) and 100mg/ kg BW (Mean= 164.60; SD= 25.04) chayote ethyl acetate fraction groups, 20 mg omeprazole group (Mean= 170.60; SD= 21.58), and 200 mg/ kg BW chayote ethanol extract group (Mean= 176.80; SD= 10.98). Conclusion: The low dose (100mg/ kg BW) chayote ethanol extract has the most potential antiinflammation effect on in vitro gastritis with the lowest IL-8 level of all doses of chayote ethanol extract, chayote ethyl acetate fraction, and omeprazole. Keywords: antiinflammation, IL-8, chayote ethanol extract, ethyl acetate fraction, omeprazole, aspirin induced gastritis Correspondence: Hendrika Andriana Silitonga. Department of Histology, Faculty of Medicine, Universitas Methodist Indonesia. Email: [email protected]. Mobile: +6281361430688. DOI: https://doi.org/10.26911/the7thicph.05.35

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COCONUT MILK MODULATE THE ANTIGENICITY OF ALPHA-LACTALBUMIN IN BALB/C MICE

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i3.15753 ◽

2017 ◽

Vol 9 (3) ◽

pp. 290 ◽

Cited By ~ 1

Author(s):

Yamina Benaissa ◽

Samia Addou ◽

Wafaa Dib ◽

Omar Kheroua ◽

Djamel Saidi

Keyword(s):

Histological Study ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Coconut Milk ◽

Cow’S Milk ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Milk Formula ◽

Cow's Milk

Objective: The aim of this work was to study the biochemical characteristics of coconut milk and its antigenic effect on the Balb/c mice immunized with α-lactalbumin protein, as well as its consequences on the structure of the intestinal epithelium.Methods: To achieve the objective of the study, an electrophoresis was realised on a polyacrylamide gel to determine various proteins contained in coconut milk. In addition, Lowry’s method was used to determine the amount of proteins in the formula. The antigenicity of coconut milk in sera was also studied using the Enzyme-Linked Immunosorbent Assay (ELISA) method. For the histological study, 21 w-old mice Balb/c were used and distributed in three groups of 7 mice each. Group 1, received a standard feed with no treatment (Negative control), group 2 and 3 received respectively a standard feed (Positive control) and coconut milk for a period of 28 d after being immunized with α- lactalbumin.Results: Analysis of the data revealed that the rate of proteins of cow’s milk is higher than that of the coconut milk ( p0.01). However, after carrying out the electrophoresis analysis, the coconut milk showed the absence of intact proteins. The anti α-Lactalbumin IgG titers significantly increased in positive control groups that received coconut milk (p<0.0001). Moreover, there was an increase of the intestinal villi height of mice fed with coconut milk, in the structure level of their intestinal epithelium compared to the negative control group.Conclusion: The findings of the study provide the evidence that coconut milk is a possible alternative to the cow’s milk formula in case of allergy.

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