scholarly journals Physical and morphological characteristics of the first three jets of Pêga jackasses sperm-rich fraction

2016 ◽  
Vol 68 (4) ◽  
pp. 845-852
Author(s):  
L.E. Carvalho ◽  
J.M. Silva Filho ◽  
M.S. Palhares ◽  
A.L.R. Sales ◽  
A.T. Gonczarowska ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Morphology ◽  
Morphological Characteristics ◽  
Open Model ◽  
Cytoplasmic Droplet ◽  
The Third ◽  
Semen Collection ◽  
Normal Sperm ◽  
Artificial Vagina

ABSTRACT The first three jets of the sperm-rich fraction of Pêga jackasses were collected and assessed separately. Five fertile Pêga jackasses were used as semen donors and underwent fractionated semen collection, using an open model artificial vagina. The first three jets of the semen were collected separately and assessed for volume, sperm motility, vigor, concentration/mL of semen, and sperm morphology. These characteristics were compared between first, second and third jets and between jackasses. It was observed that the jet volume differed (P<0.05) between jackasses, although it was similar (P>0.05) between first, second and third jets. Sperm motility did not differ (P>0.05) between jets and jackasses. Vigor was similar (P>0.05) between jets of the same jackass, and only the first jet differed (P<0.05) between jackasses. The first, second and third jets of the sperm-rich fraction had decreased sperm concentrations (P<0.05) of 955.56, 725.56 and 280.56x 106 sperm/mL of semen, respectively. Sperm morphology differed between the first three jets only for the incidence of mid-piece defect, higher in the third one (4.26%), compared to the first (3.36%) and second (3.38%) ones. When comparing the morphological characteristics of the sperm-rich fraction between five jackasses, regardless of the jet, there were differences in the percentage of normal sperm, proximal cytoplasmic droplet, mid-piece and head defects.

scholarly journals Reproductive biology of Pêga Jackasses: characteristics of sperm-rich fraction collected using the split-ejaculate method

2016 ◽  
Vol 68 (4) ◽  
pp. 832-838
Author(s):  
L.E. Carvalho ◽  
J.M. Silva Filho ◽  
M.S. Palhares ◽  
A.L.R. Sales ◽  
A.T. Gonczarowska ◽  
...  
Keyword(s):  
Sperm Concentration ◽  
Morphological Characteristics ◽  
High Quality ◽  
Open Model ◽  
Mean Values ◽  
Normal Sperm ◽  
Semen Volume ◽  
The Mean ◽  
Semen Processing ◽  
Artificial Vagina

ABSTRACT The aim of this study was to evaluate the physical and morphological characteristics of the sperm-rich fraction of jackass semen. To this end, 130 ejaculates from five Pêga jackasses were collected using an open model artificial vagina. The sperm-rich fraction was collected using the split-ejaculate method and assessed for the number of mounts/ejaculate, for physical and morphological characteristics of the semen and number of doses produced/ejaculate. It was observed that all characteristics evaluated differed among the five jackasses, except for the head defect rates. The mean values obtained for the jackass sperm-rich fraction collected were: number of mounts/ejaculate - 1.27; semen volume - 20.21mL; motility - 84.53%; vigor - 4.46; motility after dilution - 80.10%; sperm concentration/mL - 894.38 x 106; total sperm/ejaculate - 16.14 x 109; number of insemination doses/ejaculate (400x106 motile sptz) - 33.39; number of insemination doses/ejaculate (800 x 106 motile sptz) - 16.69; and percentage of normal sperm - 90.46%. Thus, in the present experiment the split-ejaculate method using an open artificial vagina worked well with the jackasses, and the sperm-rich fraction of the ejaculate of Pêga jackasses had high quality and sperm concentration, allowing its use for semen processing without reducing the number of insemination doses produced per ejaculate.

scholarly journals Establishment of semen collection technique using electroejaculator and semen cryopreservation of Javan leopard (Panthera pardus melas Cuvier, 1809)

2021 ◽  
pp. 3156-3163
Author(s):  
Bongot Huaso Mulia ◽  
Ardyta Widianti ◽  
Jansen Manansang ◽  
Dedi Rahmat Setiadi ◽  
Vincentia Trisna Yoelinda ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Morphology ◽  
Total Concentration ◽  
Sperm Concentration ◽  
Panthera Pardus ◽  
Semen Collection ◽  
Normal Sperm ◽  
Frozen Semen ◽  
Semen Volume ◽  
Microscopic Evaluation

Background and Aim: The Javan leopard (Panthera pardus melas Cuvier, 1809) is a subspecies of Panthera pardus spp., spread across the African and Asian regions. Information on reproductive aspects is crucial for wild animals, including the Javan leopard. In this study, we aimed to develop electroejaculator (EE) techniques and evaluate cryopreservation success in Javan leopard semen. Materials and Methods: The semen of four adult Javan leopards was collected once a week using EE. Placement of the EE probe in the rectum was performed after ultrasound imaging (ultrasonography) to determine the prostate body location. The semen obtained was then evaluated macroscopically and microscopically. Three Javan leopards were used for cryopreservation. The ejaculate was divided into two parts [i.e., one part diluted with AndroMed® (Minitüb, Tiefenbach, Germany) and the other part with Steridyl® (Minitüb, Tiefenbach, Germany)] at a 1:1 ratio immediately after collection and evaluation. The semen was then packed in a 0.25 mL MiniStraw® (Minitüb, Tiefenbach, Germany) then equilibrated at 4°C for 2 h. After equilibration, the straw was then frozen in liquid nitrogen vapor. Frozen semen was then stored in containers until further evaluation. Results: The results showed that ejaculation response occurred at all levels of stimulation, while erections did not always occur. The fastest ejaculation and erection occurred at the fourth voltage. The macroscopic evaluation showed that the semen volume was 0.80±0.26 mL, cloudy white, pH 7.44±0.14, and with watery semen consistency. The microscopic evaluation showed that the sperm motility was 66.98±0.39%, with sperm viability of 75.6±1.79%. Sperm concentration was 62.17±46.95×106 mL–1 with a total concentration of 42.14±23.51×106 cells. Normal sperm morphology is only 40.72±6.26%. Conclusion: This study concluded that the development of a semen collection technique using an EE preceded by imaging of the EE probe location using ultrasound was effective for the ejaculation of Javan leopards. The characteristics of the semen of the Javan leopard showed moderate semen volume, sperm motility, and viability. Javan leopard showed low sperm concentration and normal sperm morphology.

The employment of IVF techniques for establishment of sodium, copper and selenium impact upon human sperm quality

2016 ◽  
Vol 28 (10) ◽  
pp. 1518 ◽  
Author(s):  
Urszula Marzec-Wróblewska ◽  
Piotr Kamiński ◽  
Paweł Łakota ◽  
Marek Szymański ◽  
Karolina Wasilow ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Morphology ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Human Sperm ◽  
Human Semen ◽  
Glutathione Peroxidase Activity ◽  
Normal Sperm ◽  
Group I ◽  
Gpx Activity

We analysed sodium (Na), copper (Cu) and selenium (Se) levels in human semen and glutathione peroxidase activity (GPx) in seminal plasma and examined their relationships with sperm quality. Semen samples were obtained from men (n = 168) undergoing routine infertility evaluation. The study design included two groups based on standard ejaculate parameters: Group I (n = 39) with normal ejaculates (normozoospermia) and Group II (n = 129) with a pathological spermiogram. Se concentration (but not Na or Cu) and GPx activity were significantly higher in normozoospermic males than in those with a pathological spermiogram and also in males with correct sperm motility and normal sperm morphology than in asthenozoospermic and teratozoospermic males. There were significant correlations between sperm motility, Se and GPx, between rapid progressive motility and Cu, between sperm motility and Na, between normal sperm morphology and Se and Cu and between sperm concentration and Cu and GPx. Significant correlations were found between Na and Cu, between Na and Se and between Cu and Se in human semen in relation to alcohol consumption and tobacco use. Na, Cu, Se and GPx are related to sperm characteristics and male fertility and their survey could improve male infertility diagnosis.

Effect of different levels of egg-yolk on freezability of Jakhrana buck semen

2016 ◽  
Author(s):  
Narendra Kumar ◽  
B. Rai ◽  
Chetna Gangwar ◽  
S. A. Lone ◽  
Anshuman Kumar ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Egg Yolk ◽  
Osmotic Swelling ◽  
Semen Collection ◽  
Sperm Abnormality ◽  
Before And After ◽  
Different Levels ◽  
Artificial Vagina ◽  
Intensive System ◽  
Acrosomal Integrity

The present study was designed to determine the effect of different levels of egg-yolk on freezability of Jakhrana buck semen. Six healthy Jakhrana bucks (BW=30 ± 2kg, age=12 ± 0.5 month) were used for semen collection. These bucks were maintained under semi-intensive system at Jakhrana Unit of C.I.R.G. Makhdoom, Mathura. A total of 48 ejaculates (6 bucks × 8 replicates) were collected twice a week using artificial vagina. Each ejaculate was divided into 4 groups (G1, G2, G3 and G4). The G1, G2, G3 and G4 were extended with Tris-egg yolk-citric acid- fructose-glycerol (TEYCFG) extenders containing 5, 10, 15 and 20% egg yolk level, respectively. Each ejaculate was evaluated for sperm motility, viability, abnormality, and hypo-osmotic swelling (HOS) response and acrosome integrity before and after freezing. At pre-freeze stage no significant (P>0.05) difference in sperm motility and viability was found among all groups. Sperm abnormality was significantly (P<0.05) higher in G4 as compared to other groups (G1, G2, G3). The HOS response and acrosomal integrity was significantly (P<0.05) higher in G1, G2 and G3 as compared to G4. However, no significant (P>0.05) difference was observed in HOS response and acrosomal integrity among G1, G2 and G3. At post thaw stage, sperm motility, viability and HOS response was significantly (P<0.05) higher in G1 and G2 as compared to G3 and G4. Sperm abnormality was significantly (P<0.05) lower in G2 as compared to other groups. The acrosomal integrity was significantly (P<0.01) higher in G1 and G2 as compared to G3 and G4. It is concluded that 10% egg yolk in Tris based extender may be the best for successful cryopreservation of Jakhrana buck semen.

scholarly journals Comparison between different dilution rates on canine semen freezing using Tris-buffer with the addition of egg-yolk and glycerol

2005 ◽  
Vol 57 (6) ◽  
pp. 764-771
Author(s):  
A.R. Silva ◽  
R.C.S. Cardoso ◽  
L.D.M. Silva
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Sperm Morphology ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Tris Buffer ◽  
Normal Sperm ◽  
Manual Stimulation

Standardized sperm concentration and volume:volume extension were compared as dilution rates for canine semen freezing. Six proven stud dogs were submitted to two seminal collections by manual stimulation. Semen was evaluated and extended in tris plus egg-yolk and glycerol according to two different dilution rates. The first one was based on a standardized sperm concentration of 200x10(6) spermatozoa/ml and the second was a volume:volume extension at a proportion of one part semen to one part extender. Semen was frozen, stored in liquid nitrogen and thawed after one week. Sperm motility and vigor were appraised after each stage of the process and at 15 and 30min post-thawing. Sperm morphology was analyzed after collection and thawing. No differences were observed between treatments after thawing regarding sperm motility and vigor, normal sperm morphology rate or longevity. Both dilution rates can be efficiently used for canine semen freezing.

scholarly journals Urethral catheterization as an alternative method for collecting sperm in the black-footed ferret (Mustela nigripes)

2020 ◽  
Vol 8 (1) ◽  
Author(s):  
Paula Mackie ◽  
Biankha Chan ◽  
Maria Franke ◽  
Gabriela F Mastromonaco
Keyword(s):  
Sperm Morphology ◽  
Species Conservation ◽  
Feeding Tube ◽  
Urethral Catheterization ◽  
Mustela Nigripes ◽  
Semen Collection ◽  
Normal Sperm ◽  
Potential Alternative ◽  
Urine Contamination

Abstract The endangered black-footed ferret (BFF; Mustela nigripes) is an important example of the benefits of assisted reproduction in species conservation with both semen evaluation and artificial insemination using fresh and frozen sperm being successfully incorporated into the breeding program. Currently, electroejaculation (EE) is routinely utilized for semen collection in BFFs, a technique that requires custom equipment and experienced operators, and does not consistently yield viable samples in this species. In this case study, we evaluated the feasibility of urethral catheterization (UC) for semen collection, a method predominately tested in domestic and non-domestic felids, on four occasions (three BFF males). After general anesthesia with a combination of ketamine, midazolam and α2-agonist dexmedetomidine (thought to promote semen release into the urethra), a lightly lubricated, flexible feeding tube was passed into the urethral opening and advanced ~7–8 cm into the urethra. A syringe attached to the feeding tube was used to apply mild negative pressure to collect sperm. Semen samples were successfully collected on all four attempts. Sperm characteristics ranged as follows: 10.5–26.0 × 106 sperm/ml concentration, 50–90% motility and 36–61% normal sperm morphology. This is the first report of the use of UC as a potential alternative to EE in the BFF, a more field-friendly technique that is less invasive and more consistent for obtaining samples free of urine contamination.

scholarly journals Sheng Jing Decoction Can Promote Spermatogenesis and Increase Sperm Motility of the Oligozoospermia Mouse Model

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Guang Yan ◽  
Fang Tian ◽  
Peng Liu ◽  
Jianming Sun ◽  
Jianmin Mao ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Sperm Motility ◽  
Sperm Morphology ◽  
Sperm Concentration ◽  
Membrane Integrity ◽  
Testicular Tissue ◽  
Whole Body ◽  
Plasma Membrane Integrity ◽  
Normal Sperm ◽  
Sperm Plasma Membrane

Sheng Jing Decoction (SJD), as a traditional Chinese medicine prescription, is mainly be used to treat male infertility. However, the pharmacological functions and molecular mechanisms of SJD are poorly understood. In this study, we investigated the functions of SJD on spermatogenesis and sperm motility and explored the potential mechanisms involved. Here, we demonstrated that high, medium, and low doses of SJD are effective in restoring the impairments of the whole body and testicular tissue by cyclophosphamide inducing and to rescue the damage of testicular tissue cells including Sertoli cells and germ cells. SJD can partly restore the decrease in sperm concentration, sperm vitality, sperm motility, and normal sperm morphology rate in oligozoospermic mouse models. Ki67 staining analyses confirm SJD can promote testicular tissue cell proliferation. Real-time RT-PCR analyses also reveal that SJD can upregulate the expression of proliferation-associated gene Lin28a and differentiation-associated genes Kit, Sohlh2, and Stra8. SJD can also reduce the impairment of mitochondrial membrane potential (MMP) and sperm plasma membrane integrity by cyclophosphamide inducing. Our results reveal that SJD is effective in improving both sperm quantity and quality by increasing the sperm concentration, sperm vitality, sperm motility, and normal sperm morphology rate. SJD can promote spermatogenesis by upregulating the expression of the proliferation-associated gene Lin28a and the differentiation-associated genes (Kit, Sohlh2, and Stra8). SJD can sustain MMP and sperm plasma membrane integrity to increase sperm motility.

Molecular basis of adaptive evolution of sperm motility involved in in vivo fertilization of terrestrial animals

Impact ◽  
2020 ◽  
Vol 2020 (6) ◽  
pp. 73-75
Author(s):  
Akihiko Watanabe
Keyword(s):  
Sexual Reproduction ◽  
Sperm Motility ◽  
Asexual Reproduction ◽  
Acrosome Reaction ◽  
Sperm Morphology ◽  
Adaptive Potential ◽  
Selective Pressures ◽  
The Face ◽  
Genetic Profiles

One of the unifying traits of life on this planet is reproduction, or life's ability to make copies of itself. The mode of reproduction has evolved over time, having almost certainly begun with simple asexual reproduction when the ancestral single celled organism divided into two. Since these beginnings' life has tried out numerous strategies, and perhaps one of the most important and successful has been sexual reproduction. This form of reproduction relies on the union of gametes, otherwise known as sperm and egg. Evolutionarily, sexual reproduction allows for greater adaptive potential because the genes of two unique individuals have a chance to recombine and mix in order to produce a new individual. Unlike asexual reproduction which produces genetically-identical clones of the parent individual, sex produces offspring with novel genes and combinations of genes. Therefore, in the face of new selective pressures there is a higher chance that one of these novel genetic profiles will produce an adaptation that is advantageous in the new circumstances. Dr Akihiko Watanabe is a reproductive biologist based in the Department of Biology, Faculty of Science Yamagata University in Japan, he is currently working on three research projects; a comparative study on the signalling pathways for inducing sperm motility and acrosome reaction in amphibians, the mechanism behind the adaptive modification of sperm morphology and motility, and the origin of sperm motility initiating substance (SMIS).

Effect of semen collection method on sperm motility of gray wolves (Canis lupus) and domestic dogs (C. l. familiaris)

2011 ◽  
Vol 76 (5) ◽  
pp. 975-980 ◽  
Author(s):  
Bruce W. Christensen ◽  
Cheryl S. Asa ◽  
Chong Wang ◽  
Lindsey Vansandt ◽  
Karen Bauman ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Canis Lupus ◽  
Domestic Dogs ◽  
Collection Method ◽  
Gray Wolves ◽  
Semen Collection

scholarly journals Effects of Photoperiod on Epididymal and Sperm Morphology in a Wild Rodent, the Viscacha (Lagostomus maximus maximus)

ISRN Anatomy ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
A. M. Cruceño ◽  
J. C. de Rosas ◽  
M. Fóscolo ◽  
E. M. Chaves ◽  
L. Scardapane ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Reproductive Cycle ◽  
Sperm Morphology ◽  
Structural Parameters ◽  
Light And Electron Microscopy ◽  
Morphological Characteristics ◽  
Activity Period ◽  
Epididymal Sperm ◽  
Wild Rodent ◽  
Lagostomus Maximus

The viscacha (Lagostomus maximus maximus) is a seasonal South American wild rodent. The adult males exhibit an annual reproductive cycle with periods of maximum and minimum gonadal activity. Four segments have been identified in the epididymis of this species: initial, caput, corpus, and cauda. The main objective of this work was to relate the seasonal morphological changes observed in the epididymal duct with the data from epididymal sperm during periods of activity and gonadal regression using light and scanning electron microscopy (SEM). Under light and electron microscopy, epididymal corpus and cauda showed marked seasonal variations in structural parameters and in the distribution of different cellular populations of epithelium. Initial and caput segments showed mild morphological variations between the two periods. Changes in epididymal sperm morphology were observed in the periods analyzed and an increased number of abnormal gametes were found during the regression period. During this period, anomalies were found mainly in the head, midpiece, and neck, while in the activity period, defects were found only in the head. Our results confirm that the morphological characteristics of the epididymal segments, as well as sperm morphology, undergo significant changes during the reproductive cycle of Lagostomus.

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