scholarly journals Semen Production and Semen Quality of Mehsana Buffalo Breed Under Semiarid Climatic Conditions of Organized Semen Station in India

2021 ◽  
Author(s):  
Nikhil Shantilal Dangar ◽  
Balkrishna P Brahmkshtri ◽  
Niteen Deshmukh ◽  
Kamlesh Prajapati
Keyword(s):  
Sperm Motility ◽  
Linear Mixed Model ◽  
Environmental Changes ◽  
Semen Quality ◽  
Random Effect ◽  
Sperm Concentration ◽  
Season Of Birth ◽  
Production Traits ◽  
Semen Collection ◽  
Semen Volume

Abstract Semen production data comprising of 55071 ejaculates of 144 bulls from Mehsana buffalo breed was analysed. The traits under study were semen volume, sperm concentration, initial sperm motility, post-thaw sperm motility and number of semen doses per ejaculate. The objective of the present study was to assess the effect of various factors affecting semen production traits and measure the semen production potential of Mehsana buffalo bulls. Data collected of semen production traits were analysed using linear mixed model, including a random effect of bull along with fixed effect of various non-genetic factors like farm, ejaculate number, season of birth, period of birth, season of semen collection and period of semen collection. First ejaculation had higher semen volume and sperm concentration resulted in to higher number of semen doses but semen quality was better in second ejaculation. Season of birth of the bull was affecting semen quality traits. As the period of birth advances semen volume increases whereas sperm concentration decreases which reflected in persistent production of number of semen doses per ejaculate. Monsoon and summer were favorable seasons for semen collection because of higher sperm concentration which resulted in to higher semen doses per ejaculate. Additionally, Monsoon collected semen had highest volume. Hence, monsoon followed by summer season would be the favorable season for semen collection. Period of semen collection affecting all the semen production traits under study but it did not have specific trend which means managemental and environmental changes over the period have sizable influence on the traits. Results of the study will help to plan future managemental practices and breeding strategies to improve semen production traits.

scholarly journals Evaluating the ability of semen cryopreservation of Blance Blue Belge bull cattle in Vietnam

2021 ◽  
Vol 19 (2) ◽  
pp. 237-244
Author(s):  
Nguyen Huu Duc ◽  
Pham Thu Giang ◽  
Tran Thi Binh Nguyen ◽  
Nguyen Thi Mai ◽  
Bui Dai Phong
Keyword(s):  
Sperm Motility ◽  
Sperm Concentration ◽  
Joint Stock Company ◽  
Stock Company ◽  
Cattle Breeding ◽  
Semen Cryopreservation ◽  
Semen Collection ◽  
Frozen Semen ◽  
Semen Volume ◽  
Fresh Semen

The objective of this study was to determine the semen cryopreservation capacity of BBB bulls in Hanoi-Vietnam. Research conducted on the fresh semen collected from 05 BBB bulls. Results showed that semen color was normal (milky white, ivory white, ivory yellow), semen volume ranged from 6.35 mL to 7.48 mL (P <0.05), initial motility of semen ranged from 80.53% to 82.92% (P <0.05), sperm concentration in semen  ranged from 1.02 x 109 sperms/ml to 1.12 x 109 sperms/mL (P <0.05), abnormal sperm ratio ranged from 6.45% to 8.12% (P <0.05), alive sperm ratio ranged from 76.34% to 82.97% (P <0.05), sperm motility after thawing from straw semen ranged from 71.33% to 75.92% (P<0.05). In conclusion, successfully semen collection from 05 breeding BBB bulls at Hanoi Cattle Breeding Joint Stock Company, semen samples had normal color and good quantity and quality, suitable for production of frozen semen; and semen cryopreservation of straws of the 05 bull BBB semen mentioned at -196oC, sperm motility after freezing-thawing reached the economic and technical norms of 675/2014 of the Ministry of Agriculture and Rural Development.

scholarly journals Repeatability Estimates For Seminal Traits And Their Phenotypic Relationships With Testes Measurements And Performance Traits In Black Bengal Buck

1970 ◽  
Vol 37 (2) ◽  
pp. 34-41 ◽  
Author(s):  
AK Rajuana ◽  
MR Tayabur ◽  
MA Hoque ◽  
SS Husain ◽  
Z Sultana
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Body Condition ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Normal Sperm ◽  
Semen Volume ◽  
Performance Traits ◽  
Mass Activity ◽  
And Performance

Repeatability for seminal traits and their phenotypic relationships with testes measurements and performance traits in Black Bengal bucks were estimated from a total of 116 repeated observations on 15 young Black Bengal bucks. Performance traits included age, body condition and body weight of bucks. Testes measurements were testes length, breadth and volume, and scrotal circumference, while seminal traits were ejaculate volume, semen density, mass activity, sperm motility, sperm concentration, total sperm per ejaculation and percent of normal sperm. High positive correlations (ranging from 0.81 to 0.90) were found between body weight and testes measurements. Semen volume and percent of normal sperm were positively correlated with age, body condition and body weight of bucks (ranging from 0.24 to 0.60). The testes measurements were strongly and positively correlated with semen volume and total sperm per ejaculation (ranging from 0.53 to 0.61), while the correlations between testes measurement and percent of normal sperm were moderate (ranging from 0.34 to 0.44). Among seminal traits, strong correlations were found between semen volume and total sperm per ejaculation (0.81) and, between mass activity and sperm motility (0.82). Repeatability of ejaculate volume was higher (r = 0.78) which indicated that selection or culling for semen ejaculate volume could be practiced from single or few observations. It could be concluded that bucks’ age, body weight and body condition along with testes volume should be considered as selection criteria for improving semen quality and semen production of breeding bucks. DOI: http://dx.doi.org/10.3329/bjas.v37i2.9879 BJAS 2008; 37(2): 34-41

scholarly journals Raw Semen Characteristics of Three Different Indonesian Local Roosters

2016 ◽  
Vol 18 (3) ◽  
pp. 165
Author(s):  
Emilia Kamung Hambu ◽  
Raden Iis Arifiantini ◽  
Bambang Purwantara ◽  
Sri Darwati
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Mass Movement ◽  
Sperm Concentration ◽  
Semen Volume ◽  
Sperm Abnormality ◽  
Microscopic Evaluation ◽  
Semen Characteristics ◽  
Live Sperm ◽  
Better Than

Indonesia has agreat variety of roosters, either indigenous type as well as exotic and cross breed. The purpose of this experiment was to study the characteristics of semen from three types of Indonesian local roosters such as Merawang, Kampung and crosses Sentul Kampung with Kedu (SK Kedu). A total of 15 roosters consist of  Merawang roosters,  Kampung, and SK Kedu roosters were 5 each. The semen was collected 3 times a week by dorso-abdominal and cloaca massage method. The parameters evaluation was macroscopic characteristics consist of volume, color, consistency, and pH. Microscopic evaluation of semen such as a mass movement, sperm motility, live sperm, sperm abnormality and sperm concentration. Results of this experiment showed that semen volume of  Merawang  (0.40±0.26 mL) was higher (p<0.05) compare to Kampung (0.24±0.12 mL) or  SK Kedu (0.16±0.10 mL) but no difference on semen color, consistency and semen pH. There were no difference in the mass movement, sperm motility and live sperm as well as on sperm abnormality among three types of roosters.  Sperm  concentration of Merawang (4490 million mL-1) was significantly higher than Kampung (3245 million mL-1) and the SK Kedu roosters (3751 million mL-1). Its was conclude that Merawang roosters had good semen quality better than Kampung and SK Kedu roosters

scholarly journals Evaluation of Native Rooster Based on Breeding Value of trait of Macroscopic and Microscopic Semen Quality

2021 ◽  
Vol 16 (3) ◽  
pp. 228-232
Author(s):  
Sutiyono Sutiyono ◽  
E. Kurnianto ◽  
Sutopo Sutopo ◽  
D. K. Ariyanto ◽  
D. Samsudewa
Keyword(s):  
Data Analysis ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Breeding Value ◽  
Semen Collection ◽  
Native Chicken ◽  
Semen Volume ◽  
Individual Cage ◽  
Yellowish White

The aim of this research is to a determined the breeding value of native roosters based on macroscopic and microscopic semen. Ten native roosters were used in this research. Complete individual cage, semen collection tools and substance, macroscopic and microscopic tools, and substance were used in this research. The native rooster was rested for 2 weeks. Massage techniques were used for semen collection. Parameters observed were volume, pH, color, viscosity, smell, sperm concentration, and sperm livability. The semen collection was repeated 5 times. One-way analysis of variances was used for data analysis with the tools SPSS 16. The breeding value was estimated based on primary traits of semen and calculated for each rooster to make a rank. The results showed that significant parameters (P<0,05) are semen volume and sperm concentration, while the non-significant parameters (P?0,05) are pH semen and sperm livability. Semen color is yellowish-white, semen viscosity is medium-viscous and smell is spermin. The results of breeding value rank are rooster numbers 6, 7, 5, 3, 2, 4, 8, 9, 10, and 1. The conclusion is the breeding value of Native roosters is varied. The recommendation is Native chicken breeding need to apply selection used breeding value based on semen quality.

scholarly journals Breeding soundness of stud bulls

1970 ◽  
Vol 25 (2) ◽  
pp. 51-61 ◽  
Author(s):  
SP Shaha ◽  
MGS Alam ◽  
M Khatun ◽  
JU Ahmed
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Holstein Friesian ◽  
Semen Volume ◽  
Breeding Soundness

Four bulls of mixed breeds (Sahiwal × Zebu, Sindhi × Zebu, Jersey × Zebu and Holstein-Friesian × Zebu )were examined physically and their semen was evaluated in three seasons: I (Rainy): May - November, II (Winter): December - February, III (Summer): March- April. The semen volume, sperm motility, sperm concentration and percentage of dead sperm ranged from 4.1 to 7.6 ml, 56.6 to 76%, 1010.3 to 1290.7 × 106/ml and 18.4 to 24.7%, respectively. All these attributes varied significantly (p<0.05) between breeds and seasons. The variations of semen quality between breeds were not significant (p>0.05). It is suggested that the Holstein - Friesian × Zebu crossbred bull produces better quality semen than others. DOI: 10.3329/bvet.v25i2.4618 Bangl. vet. 2008. Vol. 25, No. 2, 51-61

scholarly journals PRELIMINARY OBSERVATIONS ON THE SEMINAL CHARACTERISTICS OF RABBITS RAISED IN TWO LOCATIONS IN SOUTHEASTERN NIGERIA.

2021 ◽  
Vol 22 (2) ◽  
pp. 108-112
Author(s):  
U. Herbert ◽  
C. O. Acha
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Southeastern Nigeria ◽  
Abnormal Sperm ◽  
Port Harcourt ◽  
Semen Volume ◽  
Live Sperm ◽  
Artificial Vagina ◽  
Time Of Collection

A total of sixteen rabbits sourced from two locations in Southeastern Nigeria were used for this study. Semen was collected from all the rabbits twice weekly for eight weeks using an artificial vagina while observation on the effect of time of collection on semen quality were also made. The results obtained indicated that time of collection had no significant effect (P > 0.05) on all the seminal characteristics measured. However, source of the animals showed significant effect (P < 0.05) on semen volume, sperm concentration, total sperm per ejaculate and proportion of abnormal sperm. Average semen volume, sperm concentration and total sperm per ejaculate were: 0.69±0.007 ml, 104.0±1.44 x 106/m1 and 71.40±0.90x106 respectively for the rabbits sourced from Port Harcourt. The corresponding values for rabbits sourced from Owerri were: 0.66±0.008m1, 96.02 ±1.63x106iml and 63.49±1.50 x 106 respectively. Abnormal sperm proportions were 11.05±0.22% and 1216 ± 032% for the rabbits sourced from Port Harcourt and Owerri respectively. There were no significant (P> 0.05) differences observed between locations in live sperm concentration and sperm motility. Live sperm concentration and sperm motility were 87.75±1.09% and 71.62±0.74% for the Port Harcourt group, while the values obtained for the Owerri group were 88.06±034% and 71.19±0.89% respectively.  

scholarly journals Establishment of semen collection technique using electroejaculator and semen cryopreservation of Javan leopard (Panthera pardus melas Cuvier, 1809)

2021 ◽  
pp. 3156-3163
Author(s):  
Bongot Huaso Mulia ◽  
Ardyta Widianti ◽  
Jansen Manansang ◽  
Dedi Rahmat Setiadi ◽  
Vincentia Trisna Yoelinda ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sperm Morphology ◽  
Total Concentration ◽  
Sperm Concentration ◽  
Panthera Pardus ◽  
Semen Collection ◽  
Normal Sperm ◽  
Frozen Semen ◽  
Semen Volume ◽  
Microscopic Evaluation

Background and Aim: The Javan leopard (Panthera pardus melas Cuvier, 1809) is a subspecies of Panthera pardus spp., spread across the African and Asian regions. Information on reproductive aspects is crucial for wild animals, including the Javan leopard. In this study, we aimed to develop electroejaculator (EE) techniques and evaluate cryopreservation success in Javan leopard semen. Materials and Methods: The semen of four adult Javan leopards was collected once a week using EE. Placement of the EE probe in the rectum was performed after ultrasound imaging (ultrasonography) to determine the prostate body location. The semen obtained was then evaluated macroscopically and microscopically. Three Javan leopards were used for cryopreservation. The ejaculate was divided into two parts [i.e., one part diluted with AndroMed® (Minitüb, Tiefenbach, Germany) and the other part with Steridyl® (Minitüb, Tiefenbach, Germany)] at a 1:1 ratio immediately after collection and evaluation. The semen was then packed in a 0.25 mL MiniStraw® (Minitüb, Tiefenbach, Germany) then equilibrated at 4°C for 2 h. After equilibration, the straw was then frozen in liquid nitrogen vapor. Frozen semen was then stored in containers until further evaluation. Results: The results showed that ejaculation response occurred at all levels of stimulation, while erections did not always occur. The fastest ejaculation and erection occurred at the fourth voltage. The macroscopic evaluation showed that the semen volume was 0.80±0.26 mL, cloudy white, pH 7.44±0.14, and with watery semen consistency. The microscopic evaluation showed that the sperm motility was 66.98±0.39%, with sperm viability of 75.6±1.79%. Sperm concentration was 62.17±46.95×106 mL–1 with a total concentration of 42.14±23.51×106 cells. Normal sperm morphology is only 40.72±6.26%. Conclusion: This study concluded that the development of a semen collection technique using an EE preceded by imaging of the EE probe location using ultrasound was effective for the ejaculation of Javan leopards. The characteristics of the semen of the Javan leopard showed moderate semen volume, sperm motility, and viability. Javan leopard showed low sperm concentration and normal sperm morphology.

scholarly journals Pengaruh Suplementasi Vitamin E, Mineral Selenium dan Zink Terhadap Konsumsi Nutrien, Produksi dan Kualitas Semen Sapi Simental

2014 ◽  
Vol 14 (1) ◽  
pp. 6-16
Author(s):  
Fitrah Khairi ◽  
Anis Muktiani ◽  
Yon Supri Ondho
Keyword(s):  
Vitamin E ◽  
Sperm Motility ◽  
Rainy Season ◽  
Dry Matter ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Cement Production ◽  
Semen Volume ◽  
Nutrient Consumption ◽  
Fresh Semen

(Effect of supplementation of vitamin E, Selenium and Zinc mineral against to nutrient consumption, production and quality cement simental cow)ABSTRACT. Simental bulls often experience a decline in production and semen quality in the rainy season, which have a higher humidity and high rainfall. This study aims to maintain nutrient consumption, production and semen quality Simental bulls during the rainy season through the supplementation of Vitamin E, Selenium and Zinc Minerals. The study was conducted in July-December 2012 at the Artificial Insemination Center (BIB) Unggaran. The research material used in this study were 12 males Simental cows BIB Unggaran grouped by age. The experimental design used in this study is a randomized block design (RBD) with 3 treatments T1 (ration + Vit. E), T2 (ration + Vit. E + Se), T3 (ration + Vit. E + Se + Zn) and 4 groups as replicates. Parameters measured were dry matter intake and nutrient consumption, production and semen quality. Cement production was measured from fresh semen volume during the study, whereas semen quality measured motility and concentration of spermatozoa from fresh semen. Data consumption of dry matter and nutrient intake obtained were processed using analysis of variance, followed by Duncan test. While data on production and semen quality were analyzed descriptively. The results showed that the combination of vitamin E supplementation, minerals Selenium and Zinc did not affect dry matter intake, total digestible nutriens and crude protein, but resulted in a decrease in the percentage of shelter, semen volume, sperm motility and concentration smaller than before treatment. Treatment T1, T2 and T3 resulted in a decrease in the number of shelter respectively 41,55%, 19,56% and 13,63% compared to before treatment, whereas a decrease in semen volume unchanged at 44,9 %, 43,7% and 40,99%. Sperm motility due to treatment T1, T2 and T3 respectively decreased by 55,87%, 22,10% and 13,63% compared to before treatment. In line with sperm motility, sperm concentration in treatment T , T2 and T3, respectively decreased by 49,16%, 22,85% and 14,88%. The conclusion is a combination of vitamin E supplementation, minerals Selenium and Zinc can prevent the decline in cement production, sperm motility and sperm concentration Simental bulls during the rainy season.

scholarly journals Preservation of liquid semen and Artificial Insemination in native sheep

1970 ◽  
Vol 7 (2) ◽  
pp. 305-308 ◽  
Author(s):  
S Pervage ◽  
MR Hassan ◽  
M Ershaduzzaman ◽  
MAMY Khandoker
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Conception Rate ◽  
Abnormal Sperm ◽  
Semen Volume ◽  
Native Sheep ◽  
Artificial Vagina

An experiment was undertaken to determine the conception rate of native sheep by using Artificial Insemination with liquid ram semen. The semen was collected from ram using artificial vagina and the was stored in a refrigerator (4°C) for three days. The volume of semen was extended with egg yolk citrate diluter. A total of 63 ewes were inseminated with stored liquid semen collected from 15 rams by AV method. The total number of spermatozoa, live-dead, normal-abnormal, sperm motility and the pH was observed regularly. The average semen volume per ejaculate was 0.76-1.00ml and the sperm concentration was 2.37x109 - 4.30x109 per ejaculate. The number of normal spermatozoa and the pH was almost similar irrespective of days of storage. Number of live spermatozoa and the sperm motility were reduced with the increasing age of semen. The average conception rate (%) was obtained as 63.61, 61.90, 52.38 and 47.61 with sperm in zero, 1st, 2nd and 3rd day storage respectively. Keywords: Preservation; Liquid semen; Quality; Artificial insemination; Sheep DOI: 10.3329/jbau.v7i2.4739 J. Bangladesh Agril. Univ. 7(2): 305-308, 2009

scholarly journals Cryopreservation and quality assessment of boar semen collected from bulk samples

2019 ◽  
Vol 64 (No. 5) ◽  
pp. 209-216 ◽  
Author(s):  
R Ratchamak ◽  
T Vongpralub ◽  
W Boonkum ◽  
V Chankitisakul
Keyword(s):  
Sperm Motility ◽  
Semen Quality ◽  
Sperm Quality ◽  
Sperm Concentration ◽  
Bulk Sample ◽  
Energy Status ◽  
Semen Volume ◽  
Boar Semen ◽  
Live Sperm ◽  
Fresh Semen

The purpose of this study was to examine sperm quality after cryopreservation of ejaculates collected as a bulk sample, which is routinely part of semen collection, and to compare this quality with the sperm-rich fraction in boars. Ejaculates were collected as sperm-rich fractions (SRF) and bulk samples (BE) using a gloved-hand technique. Fresh semen quality in terms of semen volume, sperm concentration, total sperm motility and pH were conventionally evaluated. Then, semen was cryopreserved using the liquid nitrogen vapour method. The post-thaw sperm quality was evaluated by assessing sperm motility, live sperm with normal apical ridge and high mitochondrial energy status, lipid peroxidation was evaluated using CASA and fluorescent multiple staining and MDA levels were determined using a spectrophotometer, respectively. In terms of fresh semen quality, sperm motility in fresh semen did not differ significantly between the two groups. The treatment with the greater mean volume (BE; P &lt; 0.05) had a lower mean sperm concentration (P &lt; 0.05); meanwhile, the mean ejaculate pH collected as BE was more basic compared with SRF (P &lt; 0.05). However, there were no significant post-thaw quality changes between sperm-rich fractions and bulk samples of semen. In conclusion, ejaculates can be collected as bulk samples without the need to classify fractions for boar semen cryopreservation.

Sign in / Sign up

Export Citation Format

Share Document