scholarly journals Variations in the L-DOPA Content, Phytochemical Constituents and Antioxidant Activity of Different Germlines of Mucuna pruriens (L.) DC.

2021 ◽  
Vol 33 (8) ◽  
pp. 1881-1890
Author(s):  
B. Rakesh ◽  
K. Hima Bindu ◽  
N. Praveen
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Mucuna Pruriens ◽  
Metal Chelating ◽  
Biochemical Assays ◽  
Dpph Method ◽  
High Antioxidant Activity ◽  
Commercially Important ◽  
Potential Activity

In this study a ‘wonder plant’ Mucuna pruriens (L.) DC., which is commercially important medicinal plant of the Fabaceae family known for its treatment in Central Nervous System disorders like Dementia, Parkinson’s, Alzheimer’s, etc. have been selected. Different germplasms have been collected to analyze the phytochemical variations between them and quantify the L-DOPA in root, stem, leaves and seeds of all the five germlines using HPLC. Along with the biochemical assays, antioxidant activity by DPPH, phosphomolybdneum method, the metal chelating and reductive potential activity of all the germplasms were studied. All parts of the plant have shown the presence of L-DOPA but, seeds have the highest quantity followed by the roots, stem and leaves. Arka Shubra seeds showed high L-DOPA content (51.9 mg/g) while the other germplasms showed L-DOPA ranging from 43-45 mg/g. Highest content of carbohydrates (258.8 mg/g) and phenolics (157.0 mg/g) was seen in the seeds of Arka Aswini. While the seeds and leaves of Arka Charaka showed high protein (332.2 mg/g) and flavonoid (10.2 mg/g) content, respectively. High proline (1.74 mg/g) was observed in the seeds of Arka Shubra. Antioxidant studies revealed that Arka Charaka and Arka Daksha to be having high reductive power and free radical scavenging activity by phosphomolybdate method while high metal chelating activity was observed in Arka Aswini (88.7%) and high antioxidant activity by DPPH method was seen in Arka Shubra (86.5%).

scholarly journals UJI AKTIVITAS ANTIOKSIDAN DARI EKSTRAK ETANOL BUNGA KANA MERAH (Canna coccinea Mill) MENGGUNAKAN METODE DPPH

PHARMACON ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 542
Author(s):  
Afrilia Veronika Rumangu ◽  
Adithya Yudistira ◽  
Henki Rotinsulu
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Ornamental Plants ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Free Radical Scavenging ◽  
Antioxidant Compounds ◽  
Dpph Method ◽  
The Family ◽  
Concentration Test

Cana plants are flower ornamental plants whose genus or margins are the only ones in the family Cannacea. This study aims to determine the activity of antioxidant compounds from ethanol extract of  Canna coccinea  Mill flower. by using the DPPH method. Flowers Canna coccinea  Mill  extracted using maceration with ethanol as a solvent. As a parameter, testing of antioxidant activity was carried out using the DPPH (1,1 – diphenyl-2-pikrilhidrazil) method which was measured using UV-Vis Spectrophotometry at a wavelength of 517nm. The result showed that ethanol of Canna coccinea Mill,flower proven to have antioxidant activity in each concentration test. The highest concentration has an free radical scavenging activity by reaching of 56,00%.Keywords: Flowers Canna coccinea Mill, Antioxidant, Extraction, DPPH ABSTRAKTanaman kana adalah tanaman hias bunga yang genus atau marganya merupakan satu-satunya dalam family Cannaceae. Penelitian ini bertujuan untuk mengetahui aktivitas senyawa antioksidan dari ekstrak etanol bunga Canna coccinea Mill. dengan menggunakan metode DPPH. Bunga Canna coccinea Mill. diekstraksi menggunakan metode maserasi dengan etanol sebagai pelarut. Sebagai parameter, pengujian aktivitas antioksidan dilakukan dengan metode DPPH (1,1-difenil-2-pikrilhidrazil) yang diukur menggunakan Spektrofotometri UV-Vis pada panjang gelombang 517 nm. Hasil penelitian menunjukkan ekstrak etanol bunga Canna coccinea Mill. terbukti memiliki aktivitas antioksidan disetiap konsentrasi pengujian. Konsentrasi tertinggi memiliki aktivitas penangkal radikal bebas dengan mencapai presentase 56,00%.Kata Kunci : Bunga Canna coccinea Mill, Antioksidan, Ekstraksi, DPPH

scholarly journals Total Phenolic Content and Antioxidative Properties of Commercial Tinctures Obtained from Some Lamiaceae Plants

2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Adam Kowalczyk ◽  
Izabela Biskup ◽  
Izabela Fecka
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Mentha Piperita ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Abts Assay ◽  
Dpph Method

The antioxidant level of commercial tinctures from three Lamiaceae plants, Salvia officinalis, Mentha piperita, and Melissa officinalis, have been determined by the Folin-Ciocalteu method, the 2.2-diphenyl-2-picrylhydracyl hydrate (DPPH) radical scavenging technique and ABTS assay. Total phenolic content was expressed as GAE (gallic acid equivalent) and ranged from 0.24 to 3.99 mg/mL. Antioxidant activity in the ABTS assay, calculated as TEAC (Trolox equivalent antioxidant capacity), ranged from 23.5 to 35.6 μmol Trolox/mL, while in the DPPH method, the EC50 value ranged from 0.04 to 0.07 mL/assay. Radical scavenging activity was correlated with total phenolic content. Correlations between ABTS and F-C methods, DPPH and F-C methods and ABTS and DPPH methods were calculated. The obtained results can be useful as additional information about the antioxidant activity of galenical preparations.

HPTLC analysis and in vitro antioxidant activity of aqueous bark extract of Erythrina variegata L.

2016 ◽  
Vol 63 (3) ◽  
pp. 143-157 ◽  
Author(s):  
Kanakasabapathy Devaki
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
High Performance ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Bark Extract ◽  
Erythrina Variegata ◽  
High Antioxidant Activity ◽  
Layer Chromatography

Erythrina variegata L. is an important medicinal plant used in the preparations of Ayurvedic formulations used against several ailments. This study was carried out to investigate the presence of secondary metabolites using phytochemical screening, high-performance thin-layer chromatography (HPTLC) fingerprinting analysis and the antioxidant potential of the aqueous bark extract of E. variegata L. The secondary metabolites and the free radical scavenging activity were analyzed using standard protocols. The results obtained in the present study revealed that E. variegata has high antioxidant activity against free radicals based on phytoconstituents.

scholarly journals Antioxidant Activity of a Geopropolis from Northeast Brazil: Chemical Characterization and Likely Botanical Origin

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Joselena M. Ferreira ◽  
Caroline C. Fernandes-Silva ◽  
Antonio Salatino ◽  
Dejair Message ◽  
Giuseppina Negri
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Chemical Characterization ◽  
Decisive Role ◽  
Northeast Brazil ◽  
Total Phenols ◽  
Botanical Origin ◽  
Plant Resin ◽  
High Antioxidant Activity

Geopropolis is a product containing wax, plant resin, and soil particles. It is elaborated by stingless bees of tribe Meliponini. Methanol extracts of sample of geopropolis produced by Scaptotrigona postica (“mandaguari”) in the state of Rio Grande do Norte (RN, northeast Brazil) were analyzed for the determination of standard parameters (total phenols, total flavonoids, and radical scavenging activity) and chemical characterization by HPLC-DAD-MS/MS analysis. The sample analyzed has high contents of total phenols and flavonoids, as well as high antioxidant activity. The constituents characterized were mainly flavonols, such as quercetin methyl ethers, and methoxychalcones. Such chemical profile is similar to the composition of a green propolis from the same area of RN, which is produced by Africanized Apis mellifera, using shoot apices of Mimosa tenuiflora, popularly known as “jurema-preta.” This finding provides evidence that “mandaguari” geopropolis and honeybee propolis have the same botanical origin in RN. The sharing of a plant resin source by phylogenetically distant bees (Apinae and Meliponinae) suggests that bee genetic factors play little role in the choice of plants for resin collection and that the availability of potential botanical sources plays a decisive role.

scholarly journals FREE-RADICAL SCAVENGING ACTIVITY LEAF EXTRACT OF LITSEA LAEVIGATA GAMBLE

2019 ◽  
pp. 96-103
Author(s):  
S. SUJATHA ◽  
T. SEKAR
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Leaf Extract ◽  
Superoxide Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Scavenging Activity ◽  
Metal Chelating

Objective: In the present study, antioxidant activity in the leaf of the pet-ether, chloroform, acetone and methanolic extracts from Litsea laevigata Gamble. Leaf was investigated by employing established in vitro studies. L. laevigata belongs to the Lauraceae family. Methods: The capability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals were determined by in vitro antioxidant assays using 2,2-diphenyl-2-picrylhydrazyl free radical (DPPH.) scavenging, reducing power assay, superoxide radical (O2*-) scavenging activity, phosphomolybdenum assay, FRAP, ABT and metal chelating activity were performed to know the antioxidant potency of the plant extract of leaves of L. laevigata. Results: Results are evaluated higher in leaf extract of L. laevigata recorded total phenol, total flavonoid, and tannin. The present state of work was designed to evaluate the phytochemical, antioxidant in the plant leaf extracts of L. laevigata. The plant L. laevigata methanolic extract of leaf showed greater IC50 antioxidant activity of DPPH assay (5.264 µg/ml) and compare to other extract, higher phosphomolybdenum reduction (164.36 mg/g), better Reducing power activity leaf in methanol (0.711%), higher ferric reducing power (4060.66MmolFe(II)E/mg), and higher in superoxide radical scavenging activity in (78.12 mg/ml). However, the better metal chelating ability was shown by the water extracts of the leaf (5.145 EDTAE/100g) compared to other solvent extracts. Conclusion: The result indicates the total phenol and antioxidant activity potential of L. laevigata.

scholarly journals Free radical scavenging activity, total phenolic and flavonoid contents of mulberry (Morus spp. L., Moraceae) extracts

2012 ◽  
Vol 66 (4) ◽  
pp. 547-552 ◽  
Author(s):  
Marija Radojkovic ◽  
Zoran Zekovic ◽  
Senka Vidovic ◽  
Drago Kocar ◽  
Pavle Maskovic
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Phenolic Components ◽  
Dpph Method ◽  
Morus Spp ◽  
Phenolic And Flavonoid

Mulberry (Morus spp. L., Moraceae) fruits, leaves, bark and branch have been used in traditional medicine as diuretic, hypoglycemic and hypotensive. The mechanism of their effects is correlated with the content of active components. Objective of this work was to evaluate and compare antioxidant properties of different extracts of two Morus species growing in Serbia: Morus alba L. (white mulberry) and Morus nigra L. (black mulberry). Potential antioxidant activity, content of antioxidant compounds (phenolics and flavonoids) and radical scavenging capacity, tested by DPPH method, were evaluated. The phenolic and flavonoid composition of different Morus extracts was determined by the HPLC method. The extracts prepared from fruits, leaves and roots of M. alba and M. nigra exhibited different characteristics. The highest extraction yield was achieved by M. alba leaves extraction (23.40%). M. nigra roots extract shown the highest total phenolics (186.30 mg CAE/g), while highest total flavonoids content (67.37 mg RE/g) was determined for M. nigra leaves extracts. In addition, black mulberry leaves extracts with the highest antioxidant activity had the highest phenolic acids contents. The dominant phenolic components in the samples were rutin and chlorogenic acid. All investigated mulberry dry extracts shown high content of phenolic compounds and significant antioxidant activity. This work contributes to knowledge of the antioxidant properties of Morus species. The obtained results may be useful in the evaluation of new dietary supplements and food products.

scholarly journals Antioxidant and Chelating Activity of NontoxicJatropha curcasL. Protein Hydrolysates Produced byIn VitroDigestion Using Pepsin and Pancreatin

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Santiago Gallegos Tintoré ◽  
Cristina Torres Fuentes ◽  
Javier Solorza Feria ◽  
Manuel Alaiz ◽  
Julio Girón Calle ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Cultures ◽  
Positive Impact ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Protein Hydrolysates ◽  
Ionization Mass ◽  
Chelating Activity ◽  
Metal Chelating ◽  
Functional Components

The antioxidant and metal chelating activities inJ. curcasprotein hydrolysates have been determined. The hydrolysates were produced by treatment of a nontoxic genotype with the digestive enzymes pepsin and pancreatin and then were characterized by fast protein liquid chromatography and reverse phase chromatography. Peptidic fractions with higher radical scavenging activity were analysed by matrix-assisted laser desorption/ionization mass spectrometry. The antioxidant activity was determined by measuring inhibition of the oxidative degradation ofβ-carotene and by measuring the reactive oxygen species (ROS) in Caco-2 cell cultures. Cu2+and Fe2+chelating activities were also determined. The hydrolysates inhibited the degradation ofβ-carotene and the formation of ROS in Caco-2 cells. The lower molecular weight peptidic fractions from FPLC had stronger antioxidant activity in cell cultures compared with the hydrolysates, which correlated with a higher content in antioxidant and chelating amino acids. These fractions were characterized by a large presence of peptides with different molecular masses. The hydrolysates exhibited both Cu2+and Fe2+chelating activity. It was concluded thatJ. curcasis a good source of antioxidant and metal chelating peptides, which may have a positive impact on the economic value of this crop, as a potential source of food functional components.

scholarly journals Antioxidant activity of NSAID hydroxamic acids

2009 ◽  
Vol 59 (2) ◽  
pp. 235-242 ◽  
Author(s):  
Marijana Končič ◽  
Zrinka Rajič ◽  
Neva Petrič ◽  
Branka Zorc
Keyword(s):  
Antioxidant Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Iron Chelator ◽  
Hydroxamic Acids ◽  
Radical Scavenger ◽  
Standard Substance ◽  
Metal Chelating ◽  
Inflammatory Drugs ◽  
Derivatives Of

Antioxidant activity of NSAID hydroxamic acidsIn the present study, seven hydroxamic acid derivatives of nonsteroidal anti-inflammatory drugs (NSAIDs) (ibuprofen, fenoprofen, ketoprofen, indomethacin and diclofenac) were found to possess significant antioxidant, radical scavenging and metal chelating activities. The most active antioxidant and radical scavenger wasN-methylhydroxamic acid of diclofenac (ANT= 88.0% andEC50= 60.1 μg mL-1). The activity of the standard substance, butylated hydroxyanisole, in the two assays wasANT= 86.9% andEC50= 18.8 μg mL-1, respectively. Ibuproxam was the strongest iron chelator among investigated hydroxamic acids (EC50= 255.6 μg mL-1), yet significantly weaker than the standard substance, EDTA (EC50= 29.1 μg mL-1). It seems that different mechanism is involved in metal chelating activity than in antioxidant and radical scavenging activity. Antioxidant and radical scavenging activities may be connected with conjugation of the nitrogen lone electron pair with the carbonyl group. On the other hand, more hydrophilic substances tend to be better iron chelators.

Optimization of preparation of rosehip tea from the area of Stara Planina Mt

Etnobotanika ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 57-69
Author(s):  
Danijela Kostić ◽  
Keyword(s):  
Antioxidant Activity ◽  
Vitamin C ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Therapeutic Effects ◽  
Bioactive Substances ◽  
Rosa Canina ◽  
Maximum Extraction ◽  
High Antioxidant Activity ◽  
Medicinal Properties

Stara Planina Mt is rich in medicinal plants, that are natural sources of various bioactive substances and have many useful medicinal properties. Rosehip, the fruit of wild rose (Rosa canina L.) is very popular and widely used. There is extensive empirical evidence of its therapeutic effects. The high content of vitamins, organic acids, phenolic compounds, carotenoids and pleasant taste define it as a valuable dietary supplement. There are different procedures for making rosehip teas. In this paper, we have optimized the process of preparation of rosehip tea from the aspect of maximum extraction and preservation of vitamin C content in tea. The antioxidant activity of aqueous extracts of rosehips, depending on the extraction time and the temperature at which the extraction is performed, was also examined..The free radical scavenging activity of the plant extracts was analyzed by using the 2.2-diphenyl-1-picrylhydrazyl (DPPH) assay. Optimal conditions are 30 minutes and a temperature of 40 °С. In this way, vitamin C is best extracted and its stability is preserved, which results in the high antioxidant activity of the tested teas.

scholarly journals Seasonal Variations of Phytochemical Content and Antioxidant Activity of Senna italica Leaves

2020 ◽  
Vol 32 (9) ◽  
pp. 2371-2374
Author(s):  
SIBUSISO C. MNCUBE ◽  
SECHENE S. GOLOLO ◽  
MOTETELO A. MOGALE
Keyword(s):  
Antioxidant Activity ◽  
Seasonal Variations ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Phytochemical Content ◽  
Four Seasons ◽  
Maximum Benefit ◽  
High Antioxidant Activity

Senna italica is a widely used medicinal plant well known for their laxative properties and high antioxidant activity, as well as possession of a variety of bioactive compounds. For its optimum usage, the current study was aimed at the determination of seasonal variations in the phytochemical content and antioxidant activity of its leaves. The leaves of Senna italica were collected during each of the four seasons (autumn, winter, spring, summer) in the year 2018. Total phenolic, total tannin, total flavonoid and total saponin contents of the leaves were determined using UV spectrophotometric method. Antioxidant activity was evaluated using the DPPH free radical scavenging activity and H2O2 reducing activity assays. Total phenolic content was recorded in higher amounts during autumn; total tannin content was in higher amounts during autumn and summer; total flavonoids were higher during autumn and summer and total saponins were higher in summer. High antioxidant activity strength demonstrated through relatively lower IC50 was shown in summer and autumn which, was consistent with the relative phytochemical contents. The current results suggest that the optimum period of harvest for maximum benefit regarding the antioxidant activity of Senna italica leaves is between summer and autumn and that its antioxidant activity strength is aligned to the accumulation of the antioxidant compounds.

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