scholarly journals COMPARATIVE STUDY OF THE CONTENT OF BIOLOGICALLY ACTIVE SUBSTANCES DASIPHORA FRUTICOSA AND СОMARUM SALESOVIANUM, GROWING IN ALTAI MOUNTAINS

2020 ◽  
pp. 189-197
Author(s):  
Elena Petrovna Khramova ◽  
Tatyana Ablulkhailovna Kukushkina ◽  
Tat'yana Mikhaylovna Shaldaeva ◽  
Serafima Yakovlevna Syeva
Keyword(s):  
Comparative Study ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Altai Mountains ◽  
High Antioxidant Activity ◽  
Ethanol Extracts ◽  
First Time ◽  
Active Substances ◽  
Dasiphora Fruticosa ◽  
Pectin Substances

A comparative study of biochemical parameters of two closely related species of plants Dasiphora fruticosa and Соmarum salesovianum (shrub and subshrub), growing in the Altai Mountains, was carried out for the first time. It is established that plants contain a rich complex of biologically active substances: flavonols, tannins, catechins, carotenoids, saponins, pectin substances. In the leaves and flowers of D. fruticosa, the content of flavonols, tannins, saponins and pectin substances is 1.1–2.5 times higher than that of C. salesovianum. Particularly significant differences in the content of pectin substances and tannins, which are mainly synthesized in the leaves of plants. By contrast, saponins and flavonols accumulate in flowers more,than in leaves. Maximum content of carotenoids identified in the leaves of plants C. salesovianum (up to 70.4 mg%), but the yellow flowers of D. fruticosa contain carotenoids more 3.2 times than the white flowers of S. salesovianum. High antioxidant activity were found in water-ethanol extracts of leaves (up to 0.85 mg/g) and flowers (up to 0.98 mg/g) of D. fruticosa, which may be due to the increased content of phenolic compounds, in particular tannins compared with the S. salesovianum.

scholarly journals PHYTOCHEMICAL STUDY OF TRIFOLIUM PANNONICUM JACQ. IN THE FOREST-STEPPE OF WESTERN SIBERIA

2020 ◽  
pp. 149-158 ◽  
Author(s):  
Yelena Petrovna Khramova ◽  
Yelena Vasil'yevna Bogolyubova ◽  
Tat'yana Ablulhailovna Kukushkina ◽  
Tat'yana Mihajlovna Shaldaeva ◽  
Galina Kimovna Zvereva
Keyword(s):  
Total Activity ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Phenolic Antioxidants ◽  
Forest Steppe ◽  
Phytochemical Study ◽  
High Antioxidant Activity ◽  
Ethanol Extracts ◽  
Active Substances ◽  
Pectin Substances

The article presents data on the content of biologically active compounds and the total activity of phenolic antioxidants in the leaves and inflorescences of the valuable forage plant Trifolium pannonicum Premier cultivar grown in different agroclimatic subareas of the Novosibirsk region – moderately warm, moist and moderately warm, not sufficiently moist. It is shown that, depending on the plant growth, biologically active substances are synthesized in differently: the content of flavonols, catechins, carotenoids and pectin substances is 1.1–1.6 times higher in plants from a more humid area. Tannins maximally (up to 17.43%) accumulate in the leaves of T. pannonicum, grown in an area with insufficient moisture. All samples of T. pannonicum variety Premier are characterized by high antioxidant activity of ethanol extracts from leaves and inflorescences (1.50–2.20 mg/g). The maximum of the total activity of antioxidants was found in the inflorescences of T. pannonicum from an area with insufficient moisture. Plant material T. pannonicum variety Premier is characterized by a fairly high content of biologically active substances.

scholarly journals COMPARATIVE STUDY OF THE CONTENT OF BIOLOGICALLY ACTIVE SUBSTANCES IN THE ABOVEGROUND ORGANS OF ATRAPHAXIS FRUTESCENS AND A. PUNGENS (POLYGONACEAE), GROWING IN SIBERIA

2017 ◽  
pp. 77-87 ◽  
Author(s):  
Вера (Vera) Андреевна (Andreevna) Костикова (Kostikova) ◽  
Евгений (Evgenij) Викторович (Viktorovich) Банаев (Banaev) ◽  
Дмитрий (Dmitrij) Константинович (Konstantinovich) Костиков (Kostikov) ◽  
Татьяна (Tat'jana) Абдулхаиловна (Abdulhailovna) Кукушкина (Kukushkina)
Keyword(s):  
Comparative Study ◽  
Reproductive Organs ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Biochemical Indices ◽  
First Time ◽  
Active Substances ◽  
Pectin Substances

The results of comparative study of the content of the biologically active substances in the aboveground organs of Atraphaxis frutescens (L.) C. Koch. and A. pungens (Bieb.) Jaub. et Spach. are presented. It is established that plants contain a rich complex of biologically active substances: flavonols, tannins, catechins, carotenoids, pectin substances. The leaves and the reproductive organs of the Atraphaxis differ in the highest content of all substances. Almost on all biochemical indices aboveground organs A. pungens surpass in A. frutescens. In leaves and reproductive organs of A. pungens the content of flavonols (up to 7.32% ‒ in reproductive organs and up to 10.10% ‒ in leaves), tannins (up to 34.30% and 27.27%, respectively) and protopectins (up to 11.29 and 7.96%), and also in leaves ‒ catechins (up to 3.92%) and in reproductive organs ‒ pectins (up to 2.63%) and carotenoids (up to 83.48 mg%) higher than at A. frutescens. The content of catechins in the reproductive organs (up to 3.78%) and in leaves ‒ carotenoids (up to 273.14 mg%) is higher in A. frutescens. Populations of Atraphaxis perspective for further pharmacological researches are allocated. Researches of biologically active substances A. frutescens and A. pungens growing in Siberia are spent for the first time.

scholarly journals Flowers of Campanula species as a source of biologically active substances

2021 ◽  
Vol 38 ◽  
pp. 00033
Author(s):  
Tatiana Fomina ◽  
Tatiana Kukushkina
Keyword(s):  
Ascorbic Acid ◽  
Western Siberia ◽  
Food Additives ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Raw Material ◽  
Forest Steppe ◽  
Wet Weight ◽  
First Time ◽  
Active Substances

The content of the major groups of biologically active substances in flowers of 7 Campanula species, cultivated in the forest-steppe of Western Siberia, were investigated for the first time. Freshly collected flowers were found to contain 0.06-0.15 % of catechins, 0.31-3.17 % of flavonols, 3.31-6.47 % of tannins, 3.10-15.57 % of pectic substances, and 0.66-16.4 mg% of carotenoids calculated per absolute dre weight of raw material. The amount of ascorbic acid was 36.9-114.0 mg% per wet weight. The data indicate that Campanula flowers are promising for the production of phenolic compounds, the development of food additives and fresh consumptions a seasoning.

scholarly journals Changes in biologically active substances of stone fruits under the influence of low temperatures

2021 ◽  
Vol 34 ◽  
pp. 06017
Author(s):  
Irina Sobol ◽  
Ludmila Rodionova ◽  
Ludmila Donchenko ◽  
Artem Stepovoy
Keyword(s):  
Low Temperatures ◽  
Beta Carotene ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Carotene Content ◽  
Plum Varieties ◽  
Physical And Chemical ◽  
Active Substances ◽  
Acid Reduction ◽  
Pectin Substances

Sufficient consumption of natural biologically active substances (BAS) – vitamins, dietary fiber, mineral compounds – reduces the risk of developing diseases associated with negative external factors. A large number of BAS are contained in the cherry plum fruits. Cherry plum is characterized by storage short term, during which quality changes are possible. Studies have been conducted on the low temperatures effect the change of BAS in cherry plum fruits. Studied objects were five cherry plum varieties grown in the Russian southern regions – Dinnaya, Zhemchuzhina, Obilnaya, Puteshestvennitsa, Neberdzhayskaya ranyaya. It was found that during storage, the physical and chemical parameters change slightly, the dry substances content decreases (within 1.3-3.2 %), sugars (5.5-7.0 %), the total acids content increases (2.8 3.3 %). The BAS content slight decrease during storage does not significantly affect the nutritional value of cherry plum fruits. The ascorbic acid reduction is 9.8-17.3 %, the anthocyanins content decreases on average from 16.9 to 19.7 %, the beta-carotene content decreases by 8.7-14.8 %, the reduction level of pectin substances is 15.75-22.2 %. Thus, the freezing method application allows to preserve valuable BAS in cherry plum fruits and use them in the future as a basis for the production of functional food products.

ECDYSTEROIDS AND FLAVONOIDS OF SILENE GRAEFFERI

2021 ◽  
pp. 175-182
Author(s):  
Elena Sergeyevna Filonenko ◽  
Larisa Nikolayevna Zibareva
Keyword(s):  
Aerial Part ◽  
Western Siberia ◽  
Botanical Garden ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
General Tendency ◽  
Vegetative Development ◽  
Promising Source ◽  
First Time ◽  
Active Substances

In this paper Silene graefferi Guss. is recommended for the first time as a promising source of flavonoids and is a well-known producer of ecdysteroids. This perennial species is adapted to the conditions of Western Siberia and has been successfully cultivated in the Siberian Botanical garden of TSU since 2015. It was interesting to study the composition and features of accumulation of biologically active substances during the growth and development of S. graefferi. It was shown that 20-hydroxyecdysone and polypodine B are present in the aerial and underground parts of introduced in culture S. graefferi plants during the entire vegetative development. An unidentified ecdysteroid was detected in the aerial part at the beginning of the vegetation and in the roots during the flowering phase. During vegetative development, common flavonoids and characteristic ones at different periods of plant development were found. The phase of maximum accumulation of ecdysteroids and flavonoids of S. graefferi is the beginning of vegetation, and the minimum is flowering. In the age dynamics the highest levels of biologically active substances in plants were noted at 2 and 3 years of life, then there is a general tendency to decrease with the age of the plant. From the aerial part of S. graefferi, 20-hydroxyecdysone, polypodine B and vitexin were isolated and identified by HPLC.

scholarly journals THE CONTENT OF BIOLOGICALLY ACTIVE SUBSTANCES WATER-ALCOHOL EXTRACTS OF ASPEN BARK

2020 ◽  
pp. 373-380
Author(s):  
Svetlana Vital'yevna Soboleva ◽  
Valeriy Mikhaylovich Voronin ◽  
Ol'ga Aleksandrovna Esyakova
Keyword(s):  
Raw Materials ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Bark Extract ◽  
Regression Equations ◽  
Toxicological Studies ◽  
Extractive Substances ◽  
Phenolic Group ◽  
Ethanol Extracts ◽  
Active Substances

The aim of this work was to study the bark of aspen growing in the suburban area of Krasnoyarsk and obtain water-ethanol extracts based on it, with different growth-regulating activity. The paper deals with the possible processing of aspen bark with the release of biologically active substances of the phenolic group. Water-ethanol solutions of aspen bark of different concentrations were used as an extractant. The regression equations and the optimum conditions of extraction of the bark of aspen with the allocation of the maximum amount of extractives. Studies have shown that aspen bark contains a variety of groups of biologically active substances, including phenolic group: tannins (tannins from 17.0 to 18.0%) polyphenols (glycosides – 0.71–0.84%), flavonoids – 0.33–0.43%; pigments: (chlorophylls from 0.63 to 0.74%; carotenoids (from 0.52 to 0.55%). The obtained mathematical models were adequate to the studied processes at 95% confidence probability. The optimal values for maximum extraction of extractive substances: the concentration of ethanol was 65%; the duration of the process – 3.5 hours, hydro – 15. In the optimal mode was obtained aspen bark extract, its characteristics coincide with the requirements of TU 9377-162-20680882-10 "raw Materials for the production of biologically active additives "aspen bark Extract "thick". It was established that water-ethanol extracts of aspen bark have depending on the concentration of growth-inhibiting or stimulating activity. In the range of concentrations from 0.1 to 10 g/l, they have an inhibitory effect, with further dilution from 1∙10-2 to 1∙10-4 g/l, a stimulating effect is observed. The tendency of increasing the growth of seedlings of cereals, %: corn – 30, barley – 40 and wheat – 35; legumes, %: peas – 35, beans – 45 and lentils – 25 compared with the control at a concentration of extractive substances of aspen bark 1∙10-3 g/l. the Data obtained may be useful after appropriate sanitary-toxicological studies to increase the germination and germination of seeds of legumes and cereals.

scholarly journals Comparative study of phenolic compounds of the herb of Betonica L. genus species of flora of Ukraine

2021 ◽  
pp. 66-75
Author(s):  
Iryna Sas ◽  
Andrii Hrytsyk ◽  
Taras Koliadzhyn ◽  
Oleh Koshovyi
Keyword(s):  
Phenolic Compounds ◽  
Comparative Study ◽  
Hydroxycinnamic Acids ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
New Drugs ◽  
Raw Material ◽  
Wide Range ◽  
Nitrogen Containing Compounds ◽  
Active Substances

Species of Betonica L. genus are widespread in Ukraine and contain different groups of biologically active substances: hydroxycinnamic acids, flavonoids, tannins, iridoids, terpenoids, steroids, essential oil, organic acids, vitamin K, nitrogen-containing compounds, phenylethanoid glycosides. Species of Betonica L. genus show a wide range of pharmacological activity (anti-inflammatory, antioxidant, choleretic, diuretic, sedative, antitumor, antihypertensive, etc.) and phenolic compounds are one of the most important and promising groups of biologically active substances of these plants. The aim. The aim of the work was to conduct a comparative study of the phenolic compounds of the herb of Betonica L. genus species of flora of Ukraine. Materials and methods. The object of the study was the herb of Betonica peraucta and Betonica brachydonta harvested in the phase of mass flowering of the plant in Ivano-Frankivsk region. The study of phenolic compounds was carried out by paper chromatography, HPLC and spectrophotometry. Results. 7 components of tannins, 4 flavonoids, 5 hydroxycinnamic acids, 2 coumarins were identified and quantified by HPLC in the studied raw material. The quantitative content of the main groups of phenolic compounds in the herb of Betonica peraucta and Betonica brachydonta was determined by the method of absorption spectrophotometry: polyphenols – 5.96 % and 4.82 %, tannins – 1.62 % and 0.68 %, flavonoids – 2.07 % and 1.13 %, hydroxycinnamic acids – 7.01 % and 3.58 %, respectively. Conclusions. As a result of the conducted studies it was found that the content of phenolic compounds in the herb of Betonica peraucta is significantly higher than in the herb of Betonica brachydonta. Therefore, this species is promising for further research and creation of new drugs

scholarly journals BIOLOGICAL ACTIVE SUBSTANCES AND ANTIOXIDANT ACTIVITY OF SIBIRAEA ALTAIENSIS (LAXM.) SCHNEID. (ROSACEAE)

2019 ◽  
pp. 181-190
Author(s):  
Vera Andreyevna Kostikova ◽  
Tat'yana Abdulkhailovna Kukushkina ◽  
Tat'yana Mikhaylovna Shaldaeva ◽  
Elena Petrovna Khramova ◽  
Serafima Yakovlevna Syeva
Keyword(s):  
Antioxidant Activity ◽  
Growing Season ◽  
Biologically Active ◽  
Stage Of Development ◽  
Plant Organ ◽  
Ethanol Extracts ◽  
First Time ◽  
Maximum Content ◽  
Active Substances ◽  
Biological Active Substances

The content of biologically active substances and the level of antioxidant activity in the aerial organs of different sexual forms of Sibiraea altaiensis (Laxm.) Schneid. during the growing season were investigated for the first time. It was found that the maximum content of biologically active compounds varies depending on the stage of development, plant organ and also gender. The amount of flavonols (5.69%), tannins (30.17%) is higher in the leaves of male plants during the budding of plants, catechins ‒ in inflorescences (0.61%) of female plants, pectins ‒ in male buds (1.54%), protopectins ‒ in the leaves of males (8.99%) in the budding phase and in the leaves of females (9.62%) in the fruiting phase of plants and carotenoids ‒ in the leaves of males (70.6 mg%) and female (61.86 mg%) plants in the fruiting phase. Stalks of S. altaiensis contain quite a high amount of tannins (20.1%), pectins (1.49%), protopectins (5.93%) and carotenoids (17.37 mg%). The highest (antioxidant activity) AOA was detected in water (2.03 mg/g) and water-ethanol (1.75 mg/g) extracts from the leaves of male S. altaiensis plants. The AOA of water and water-ethanol extracts from the aerial organs of S. altaiensis is authentically positively connected with the content of all the studied substances, except for pectins.

scholarly journals EDIBLE ONION FLOWERS (ALLIUM L.) AS A SOURCE OF BIOLOGICALLY ACTIVE SUBSTANCES

2021 ◽  
pp. 291-297
Author(s):  
Tat'yana Ivanovna Fomina ◽  
Tat'yana Abdulkhailovna Kukushkina
Keyword(s):  
Phenolic Compounds ◽  
Raw Materials ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Raw Material ◽  
Forest Steppe ◽  
Dry Matter Content ◽  
Total Sugars ◽  
Active Substances ◽  
Pectin Substances

The results of comparative study of the content of biologically active substances in the flowers of 10 species of perennial onions Allium aflatunense B. Fedtsch., A. altaicum Pall., A. flavum L., A. microdictyon Prokh., A. nutans L., A. obliquum L., A. ramosum L., A. rosenbachianum Regel, A. schoenoprasum L., A. senescens L. var. glaucum Regel (A. senescens ssp. glaucum (Schrad.) N. Friesen) are presented. The freshly collected raw materials in the phase of mass flowering were analyzed. The dry matter content was determined by drying 1 g of raw material at 100–105 °C to a constant mass. The amount of phenolic compounds, pectin substances, total sugars, and carotenoids was determined spectrophotometrically using SF-56 (Russia) and SF "Agilent" 8453 (USA). For the first time, data on the quantitative content of the main groups of secondary metabolites in onion flowers were obtained. It has been established that in the forest-steppe of Western Siberia, flowers of Allium species contain: 10.7-22.5% dry substances, 6.6-26.2% total sugars, 3.3–18.6% pectin substances, 3.6–10.6% phenolic compounds, 3.0–23.4  mg% carotenoids (per mass of absolutely dry materials) and 35.4–157.8 mg% ascorbic acid (per mass of raw materials). The highest values were found for A. rosenbachianum, A. flavum, and A. aflatunense. The high variability of the accumulation of biologically active substances is due to the species and the influence of weather conditions during the growing season. The study showed the prospects for using onion flowers as a source of various bioactive compounds, including when added to food.

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