scholarly journals Effects of tributyltin chloride (TBTCl) antifouling biocide on adult males and females of brine shrimp (Artemia salina)

2017 ◽  
Vol 5 (1) ◽  
pp. 30
Author(s):  
Najla Mohamed Abu Shaala ◽  
Syaizwan Zahmir Zulkifli ◽  
Ahmad Ismail ◽  
Mohammed Noor Amal Azmai ◽  
Ferdaus Mohamat-Yusuff ◽  
...  

Elevation of tributyltin (TBT) concentration in marine environment could affect targeted and non-targeted organisms at any lifestage. The present study aimed to determine median lethal concentration (LC50) and morphological effects of tributyltin chloride (TBTCl) on adult males and females of brine shrimp (Artemia salina). The adult males and females of A. salina were exposed to different concentration of TBTCl. Morphological condition of every A. salina individuals were observed under a microscope. Results showed the LC50 of TBTCl among adult males of A. salina was 146.99 ng.L-1 and for the females was 94.72 ng.L-1, respectively. The LC50 of TBTCl was significantly different among different sexes. There was also a significant difference in some morphological characters of males and females exposed to different TBTCl concentrations. These morphological changes include their total length, head width, abdominal width, and tail width after the 24hr exposure to TBTCl. These results suggested that suspensions of the TBTCl were toxic to Artemia, most likely due to the formation of benign TBTCl aggregates in water. However, the mortality increased with extended exposure to 24hr. Highest mortality occurred at 200 ng.L-1 TBTCl; 43.33% for male and 90% for female (LC50<150 ng.L-1) for both. Depended on this the female was more sensitive for TBTCl toxicity test when compared to male. These effects were attributed to changes in morphological characteristics of the body A. salina.

2018 ◽  
Vol 25 (4) ◽  
Author(s):  
V. A. Yevstafyeva ◽  
V. V. Stybel ◽  
T. A. Sharavara ◽  
V. V. Melnychuk ◽  
I. О. Yasnolob ◽  
...  

The abundance of Capillaria obsignata Madsen, 1945, the causative agent of chicken capillariasis, was studied in the central region of Ukraine. Differential morphometric characters are determined for adult males and females of C. obsignata. The species-specific variability of its morphological parameters is characterized. New data is received on the meristic characters of C. obsignata eggs extracted from different substrates. The stages of embryonic development are determined in laboratory cultures of these nematodes, taking into account their meristic and morphological changes. Capillaria obsignata parasites are very common at the poultry farms of Poltava and Kyiv regions of Ukraine. Their abundance index is 3.4, and the intensity of infection varies from 3 to 317 specimens. Differential morphometric species-specific characters of adult C. obsignata males are the shape and size of the pseudobursa, spicule, spicule sheath. Adult females of the species can be identified by the shape and size of the vulva, the distance from the esophagus end to the vulva, and the length of the vagina. The meristic characters of nematode eggs, though typical for the species, may change depending on the sampled substrate. The parasite’s embryonic development involves five morphologically and meristically different stages. The invasive larvae of this species developed in the eggs before the 12th day of culturing in laboratory conditions at 25 °С. The invasive larvae’s viability was 90.3 ± 1.5%.


2019 ◽  
Vol 53 (4) ◽  
pp. 325-334
Author(s):  
V. N. Peskov ◽  
N. A. Petrenko ◽  
V. Yu. Reminnyi

Abstract We study size-at-age and sexual variability of morphometric characteristics of the marsh frog. According to the size of the body, males were divided into three size-age groups (juvenis, subadultus, adultus), females — into four groups (juvenis, subadultus, adultus, adultus-I). We found that the chronological age of frogs (skeletochronology) does not always correspond to their biological age (size and proportions of the body). We noted that the semi-adult males are reliably larger than females by mean values of 26 studied morphometric characters. Males and females of “adultus” group do not differ by linear body size, significant differences were found in body proportions (7 characters). For the females of “adultus-I” group, the mean values of 26 characters are significantly larger than for “adultus” males. The results of our study showed that with the age of the marsh frog, the level of exhibition, directionality and structure of morphometric sex differences changes.


1973 ◽  
Vol 58 (3) ◽  
pp. 643-649 ◽  
Author(s):  
H. Schmitt ◽  
H. Grossfeld ◽  
U. Z. Littauer

Mitochondria isolated from cysts of Artemia salina (brine shrimp) were found to be devoid of cristae and to possess a low respiratory capability. Hydration of the cysts induces marked biochemical and morphological changes in the mitochondria. Their biogenesis proceeds in two stages. The first stage is completed within 1 h and is characterized by a rapid increase in the respiratory capability of the mitochondria, their cytochrome oxidase, cytochrome b, cytochrome c and perhaps some morphological changes. In the second stage there is an increase in the protein-synthesizing capacity of the mitochondria as well as striking changes in mitochondrial morphology leading to the formation of cristae.


Author(s):  
M. Barson

Clarias gariepinus were collected from Lake Chivero, Zimbabwe, and examined for nematode parasites from November 2000 to May 2002. Of the 202 specimens collected, 42.6 % were infected with third-stage larvae of Contracaecum sp. in the body cavity. The intensity of the infection was 1-7 worms per fish (mean intensity = 2.2). Seasonal variation in the prevalence of the parasite was not obvious and there was no significant difference in the prevalence of infection between males and females (c2 = 2.228; P > 0.05). No significant relationship between host size and prevalence was established. There was also no significant relationship between intensity and the body condition factor (r = 0.11; P > 0.05). The low parasite prevalence may have been caused by the disruption of the infection cycle since piscivorous birds, which are the final hosts of the parasite, do not feed on C. gariepinus in Lake Chivero.


2018 ◽  
Vol 16 (2) ◽  
pp. 78-81
Author(s):  
Biswas Satyal ◽  
Abhishek Satyal

Introduction: The ratio of the lengths of the index and the ring finger (2D:4D ratio) is generally different between men and women. A number of studies have shown a correlation between the 2D:4D digit ratio and various physical and behavioral traits. The aim of the present study is to investigate the association of the index (2nd) and ring (4th) digit ratios with some physical traits in Nepal population. Material & Method: 200 students (100 males and 100 females) between ages of 18 years and above were randomly selected with exclusion of those with hand deformities. The digit lengths were measured from the basal crease to the tips usingvernier calipers. The 2D:4D ratios were determined for each subject while height and weight were used to calculate the body mass index and data analyzed. The study was conducted between January 2018 to November 2018. Result: The result of the anthropometric study of the differences in index (2D) and ring (4D) and their ratios shows that there was a significant difference between the length of index finger (2D), ring finger (4D) and the ratios of right hand's 2D:4D in both males and females. There was appositive correlation between the second digit length and Height and weight in males and females both on right and left sides. The 2D:4D ratio for both left and right hand did not show any positive correlation with height, weight or BMI of an individual.


1987 ◽  
Vol 114 (2) ◽  
pp. 178-184 ◽  
Author(s):  
Hajime Watanobe ◽  
Kazuo Takebe

Abstract. The cimetidine-induced plasma Prl response was examined in rats of both sexes. First, 10 week old intact adult males and females (dioestrous) were compared. There was no significant difference in the Prl response to cimetidine between the two groups, despite the fact that in adult females the anterior pituitary Prl content was 4 times greater than in males. Second, the effect of gonadal state in adult age on the Prl response to cimetidine was examined in both sexes. In male rats, gonadectomy at the age of 6 weeks significantly reduced the plasma Prl response as well as the pituitary Prl content, both of which were sufficiently restored by testosterone replacement. However, in females, neither gonadectomy at the age of 6 weeks nor subsequent oestradiol replacement affected the Prl response to cimetidine, despite the fact that gonadectomy significantly reduced and oestradiol treatment significantly enhanced the pituitary Prl content. Third, possible permanent effects of the postnatal gonadal milieu on the cimetidine-induced Prl response and the pituitary Prl content were examined in both sexes by castration at varying postnatal ages. The ratio of plasma Prl response to pituitary Prl content was similar in all castrated males. In females, however, the ratio decreased with increasing castration age. In conclusion, the mechanism of cimetidine-induced Prl release is less sex-dependent than are the mechanisms of Prl release by other Prl secretagogues. First, this may be due to a minor role of oestrogen in females in determining the Prl response to cimetidine. Second, the postnatal ovarian secretions may exert a permanent inhibition of the development of the cimetidine-mobilized anterior pituitary Prl pool.


2015 ◽  
Vol 1 (1) ◽  
pp. 5-12 ◽  
Author(s):  
Alexander Čanády ◽  
Ladislav Mošanský ◽  
Peter Krišovský

Abstract Sexual dimorphism of the Eurasian red squirrel on five somatic variables (head-and-body length, tail length, hind foot length, ear length and body weight) was evaluated on 55 squirrels. The overlap of values of the measured traits among sexes was high, but in all traits (with exception of the tail length) males seem to exhibit slightly higher mean values than females. However, these differences were non-significant, with the exception of a small significant difference in the tail length and tail-to-head-and-body ratio. Similarly, the results of discriminant function analysis show no differences between the sexes. Positive correlation analyses, together with PCA values, confirmed that tail length and hind foot length are traits that play a significant role in overall variability. We suggest that this relationship could explain their mutual importance in locomotion, where the feet are essential for movement in the trees. By contrast, the tail maintains balance on thin branches, or during jumps from one crown to another. Longer tails also demonstrate differential selection on males and females for a locomotor trait. Similarly, we discuss whether variations in tail length were connected to female reproductive success. Our results suggest that the non-significant results regarding SSD provide the benefit of the same size for both sexes in the protection of territory as well as inter- and intra-sexual interaction.


2007 ◽  
Vol 40 ◽  
pp. 55-64 ◽  
Author(s):  
M. Al-Amin ◽  
A. Nahar ◽  
A.K.F.H. Bhuiyan ◽  
M.O. Faruque

SummaryNorth Bengal Grey (NBG) cattle are an important indigenous cattle genetic resource found mainly in the northern part of Bangladesh. The study was undertaken at Bogra Sadar, Shibgonj and Kahalu Upazila (sub-district) in the Bogra district. The physical and morphological characteristics, and the productive and reproductive performances of NBG cattle were studied. The coat colour of these animals is deep grey to white. The coat colour of the neck region in adult bulls was found to be generally ashy with a range of shades.The body is small, compact and less fleshy. Ear length and ear width were 18.0±0.17 and 11.0±0.21 cm, respectively. The head length average was 38.0±0.56 cm, the head width 16.0±0.17 cm, the foreleg length average 65.0±0.64 cm, the hind leg length 71.0±0.64 cm, the tail length average 71.0±0.67 cm, the horn length average 9.0±0.39 cm, the horn diameter 10.0±0.37 cm, the average teat length 5.0±0.18 cm, the teat diameter 6.0±0.22 cm, the distance betweenthe front teats 7.0±0.13 cm and the distance between the rear teats 7.0±0.13 cm. Body length, height at wither and heart girth in adult cows were 105.0il.20, 94.0+1.12 and 127.0±1.52 cm, respectively.The recorded highest peak milk production per day was 3.5±0.18 kg, lactation length was 219±6.1 days, and the dry period was 180±6.8 days. The average birth weight of calves was 18.4±0.52 kg and mature live weight of cows 241.0±4.0 kg. The age at first heat was 869±29.6 days, age at first calving 1191±19.7 days, gestation length 281±1.3 days, calving interval 442±7.4 days, postpartum heat period 110±4.2 days and the number of services per conception 1.4±0.6. About 54% of total cattle population was NBG cattle in the surveyed area of Bangladesh. The results indicated that the productive and reproductive performance of NBG cattle was better than other non-descript indigenous cattle of Bangladesh. The study further revealed an obvious need for more in-depth and objective information on wider samples of this type of indigenous cattle in order to assess the future need for conservation and improvement programs to be undertaken.


2017 ◽  
Vol 268 ◽  
pp. 254-258 ◽  
Author(s):  
Nur Shafawati Rosli ◽  
Azhar Abdul Rahman ◽  
Azlan Abdul Aziz ◽  
Shaharum Shamsuddin ◽  
Nurul Sabihah Zakaria

Ultrastructural characteristic and morphological changes of untreated and treated breast cancer MCF-7 cells were observed by energy-filtered transmission electron microscope (EFTEM). Morphological observation of MCF-7 after being treated with 13 nm, 50 nm, and 70 nm AuNPs, were looking unhealthy and dying out of the populace, the observed cells were more reduced and dying as treatment with 50 nm and 70 nm AuNPs. Cells detachment, clumping, shrunken, and dispersed cells in the culture medium and floating cells were also observed. The observed morphological changes increase in 50 nm and 70 nm AuNPs than in 13 nm AuNPs, which is less toxic to MCF-7 cells. The presented morphological analysis has established that 13 nm AuNPs showed less toxic to MCF-7 breast cancer cells. Whereas, control cells of MCF-7 were treated with only complete culture media, despite the duration of treatment, whereby the cells maintained most of their morphological features and observed to have a typical morphology of healthy cells that are well attached to the surface. These results indicate that AuNPs were clustered in the cells and there was no significant difference between images of different sizes of AuNPs observed in the cells, because the AuNPs always clustered together inside the cells.


1987 ◽  
Vol 8 (1) ◽  
pp. 13-17 ◽  
Author(s):  
S.P. Gittins

AbstractThere was no significant difference between the number of food items obtained by stomach-flushing toads caught in pitfall traps and from the dissection of corpses found on nearby roads. The frequencies of the different food categories were similar using the two methods. 97 % of the toads entering the pond in the spring had not recently fed, whereas 25% of toads leaving the pond had started to feed. Sloughed skin was found in 7% of stomachs. Vegetable matter was found in 15% of stomachs and inorganic matter in 6% of stomachs. The diet of the toad was found to be very varied, and the main food items were beetles, collembolans, millipedes, harvestmen and spiders. The diets of males, females and young toads were very similar, and only slight changes in diet were observed throughout the year. The size of food items ranged from 1 mm to 40 mm with most around 7 mm. There was no significant relationship between the size of the food item and the size of the toad for adult males and females, but there was for juveniles.


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