scholarly journals PO-078 Effects of aerobic and resistance training on cardiac diastolic dysfunction in type 2 diabetic rats

2018 ◽  
Vol 1 (3) ◽  
Author(s):  
Shunchang Li ◽  
Derun Gao ◽  
Ismail Laher ◽  
Quansheng Su
Keyword(s):  
Diabetes Mellitus ◽  
Resistance Training ◽  
Protein Expression ◽  
Diastolic Dysfunction ◽  
Aerobic Training ◽  
Diabetic Rats ◽  
Collagen I ◽  
Male Rats ◽  
Type I

Objective To compare the effects of two types of physical training on cardiac diastolic dysfunction in type 2 diabetes mellitus and investigate the role of passive tension regulators—titin and collagen. Methods Sprague-Dawley (SD) male rats were divided into two groups: control and diabetes. Each group was subdivided into three subgroups: sedentary rats, rats submitted to aerobic trained and rats submitted to resistance training. Diabetic rats were induced by high-fat diet combined with low-dose injections of streptozocin (STZ). Aerobic trained rats ran on a treadmill at 21m/min for 60 min per day, while resistance trained rats climbed a ladder with incremental loads. The train period lasted for 8 weeks. Results All diabetic rats had increased fasting blood glucose (FBG) and left ventricular end diastolic pressure (LVEDP), a lower down stroke of the pressure curve (dp/dt min), longer exponential time constants of relaxation (Tau) in relation to control rats, while the protein expression of titin was decreased significantly, and the expression of collagen I, TGFβ1 were increased slightly, the ratio of type I and III collagen was raised in diabetic rats. Both types of training promoted a decrease in FBG and HOMA-IR in diabetic rats. Aerobic exercise trained diabetic rats had significant higher –dp/dtmin, and shorter Tau, but -dp/dtmin and Tau in resistance exercise trained diabetic rats had no significant been improved, even more aggravation. Moreover, aerobic training increased the protein expression of titin and HSP27, and the amount of co-localization of titin and HSP27 elevated, the protein expression of collagen I, TGFβ1 were decreased, and the ratio of type I and type III got close to normal. Resistance training further decreased the expression of titin, collagen I, and TGFβ1, collagen volume fraction (CVF) was increased significantly, and the ratio of type I and III collagen was disturbed, but the co-localization of titin and HSP27 elevated slightly. Conclusions Aerobic training ameliorates cardiac diastolic dysfunction in diabetes and this improvement may be related to increase titin repaired by HSP27, while resistance training aggravates the cardiac diastolic dysfunction in early diabetes mellitus and it could be caused by worsening myocardial interstitial fibrosis.

scholarly journals Glycosylated hemoglobin and left ventricular diastolic dysfunction in patients with type 2 diabetes mellitus

2017 ◽  
Vol 5 (12) ◽  
pp. 5330 ◽  
Author(s):  
Vishal S. Yesankar ◽  
Nalini R. Humaney
Keyword(s):  
Diabetes Mellitus ◽  
Diastolic Dysfunction ◽  
Glycosylated Hemoglobin ◽  
Left Ventricular Diastolic Dysfunction ◽  
Left Ventricular ◽  
Type I ◽  
Type Ii ◽  
Chi Square ◽  
Ventricular Diastolic Dysfunction

Background: Diabetes mellitus is one of the most common diseases. Left ventricular diastolic dysfunction may represent the early stage of diabetic cardiomyopathy thus reinforcing the importance of the early examination of diastolic function in individuals with diabetes.Methods: This is a hospital based cross-sectional study done at a tertiary care hospital catering mainly to rural population. Patients having type 2 diabetes mellitus were scrutinized for doppler echocardiography and HbA1c levels. As per the previous studies and considering the prevalence of asymptomatic diastolic dysfunction in diabetics, the sample size was calculated. Statistical analysis was done by using descriptive and inferential statistics using chi square test.Results: In the present study 45 patients had HbA1C between 6.5- 8.0, out of which 9 had type I diastolic dysfunction, 1 had type II diastolic dysfunction and 35 had no diastolic dysfunction. 16 patients had HbA1C between 8.1- 9.5, out of which 7 had type I diastolic dysfunction, 8 had type II diastolic dysfunction and 1 had no diastolic dysfunction. 14 patients had HbA1C more than 9.5, out of which 4 had type I diastolic dysfunction, 5 had type II diastolic dysfunction, 4 had type III diastolic dysfunction and 1 patient did not had any diastolic dysfunction. The Chi-Square value is 55.51 and p value is 0.000 (Significant).Conclusions: Diastolic dysfunction correlates with the levels of glycosylated hemoglobin, duration of diabetes mellitus, presence of microvascular complications like diabetic retinopathy, neuropathy and nephropathy.

scholarly journals THE IMPACT OF QUERCETIN ON THE FUNCTIONAL STATE OF CARDIOVASCULAR SYSTEM AND HEMOSTASE IN RATS WITH TYPE 2 DIABETES MELLITUS

2021 ◽  
Vol 77 (3) ◽  
pp. 105-110
Author(s):  
Olha Ivanova
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Diastolic Dysfunction ◽  
Cardiovascular System ◽  
Functional State ◽  
Cardiovascular Complications ◽  
Diabetic Rats ◽  
The Impact

Introduction. Type 2 diabetes mellitus (T2DM) increases the risk for atherosclerotic cardiovascular disease and other cardiovascular complications such as cardiac arrhythmias, heart failure, and thrombotic events. Quercetin (Q) possesses a wide range of multiple activities: anti-diabetic, anti-proliferative, anti-atherosclerotic, antioxidant, anti-inflammatory, anti-thrombotic, anti-apoptotic effects and is regarded as a candidate for the role of cardiovascular complications protecting agent. The aim of this study was to assess the effects of Q on the functional state of cardiovascular system and haemostasis in diabetic rats. Materials and Methods. T2DM was induced in Wistar rats by a high-caloric diet during 14 weeks combined with intraperitoneal injections of 25 mg/kg streptozotocin twice per week. All diabetic animals were divided into three groups: treated with solvent and with Q (in dose 10 and 50 mg/kg/day per os) for 8 weeks after diabetes induction. Fibrinogen concentration and induced euglobulin fibrinolysis time were measured in plasma using reagent kits. Electrocardiograms were recorded in leads II. Results. It was established that Q in dose 50 mg/kg b.w. prevents in the formation of sinus tachycardia in experimental animals. In addition, Q in both doses inhibits the development of myocardial diastolic dysfunction, which was confirmed by prolongation of T-P interval and a decrease of duration of the T wave in comparison with diabetic rats. Q in both doses restorated the processes of coagulations and fibrinolysis, as indicated by a decrease of fibrinogen levels and the time of thrombolysis compared to diabetic rats. Conclusions. Q, independently of dose, inhibits the development of myocardial diastolic dysfunction and reduces prothrombotic potential in rats with type 2 diabetes, which may ameliorate diabetic cardiovascular risk. This data justify the perspective of Q for the prevention and management of cardiovascular complications in patients with type 2 diabetes.

scholarly journals Beneficial Effects of Soygurt Intake in Type 2 Diabetes Mellitus in Animal Model Rat (Rattus Norvegitus)

2020 ◽  
Vol 1 (1) ◽  
Author(s):  
Ni Wayan Desi Bintari ◽  
Putu Ayu Parwati
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Body Weight ◽  
Blood Sugar ◽  
Blood Sugar Level ◽  
Diabetic Rats ◽  
Male Rats ◽  
Probiotic Food

Type 2 Diabetes Mellitus (T2DM) is the more common type of diabetes results from the ineffective use of insulin. Improvement of the metabolic system in T2DM patients can be done through the regulation of gut microbiota balance. Gut microbial improvement can be modulated directly by probiotic food consumption. Soygurt is probiotic food with a low glycemic index (GI) and glycemic load (GL) value and rich in isoflavones, which has a potential effect in reducing diabetes risk. The aim of this study is to determine the effect of soygurt consumption in blood glucose levels and body weight of albino wistar rats (Rattus norvegitus). Reseach using a completely randomized design for experimental study. Subjects of this research are 30 male rats (R. norvegistus) aged 2-3 months with average body weight 150-200 gr. Diabetic rats were induced by using single intraperitoneal injection (175 mg/kg BW) alloxan monohydrate. Soygurt feeding given once daily using oral gavage feeding. The result showed that soygurt feeding in diabetic rats with three variations of treatment could significantly (p<0,05), lowering blood sugar level and improve body weight after 28 days of treatment. Treatment of 4ml/day soygurt has the highest effect in lowering blood sugar level and improving body weight, followed by treatment of 3ml/day and 2ml/day soygurt.

scholarly journals The Biological Impacts of Sitagliptin on the Pancreas of a Rat Model of Type 2 Diabetes Mellitus: Drug Interactions with Metformin

Biology ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 6 ◽  
Author(s):  
Lamiaa M. Shawky ◽  
Ahmed A. Morsi ◽  
Eman El Bana ◽  
Safaa Masoud Hanafy
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Rat Model ◽  
Cell Damage ◽  
Diabetic Rats ◽  
Male Rats ◽  
Control Group ◽  
Dipeptidyl Peptidase 4

Sitagliptin, a dipeptidyl peptidase-4 (DPP-4) inhibitor, is a beneficial class of antidiabetic drugs. However, a major debate about the risk of developing pancreatitis is still existing. The aim of the work was to study the histological and immunohistochemical effects of sitagliptin on both endocrine and exocrine pancreases in a rat model of type 2 diabetes mellitus and to correlate these effects with the biochemical findings. Moreover, a possible synergistic effect of sitagliptin, in combination with metformin, was also evaluated. Fifty adult male rats were used and assigned into five equal groups. Group 1 served as control. Group 2 comprised of untreated diabetic rats. Group 3 diabetic rats received sitagliptin. Group 4 diabetic rats received metformin. Group 5 diabetic rats received both combined. Treatments were given for 4 weeks after the induction of diabetes. Blood samples were collected for biochemical assay before the sacrification of rats. Pancreases were removed, weighed, and were processed for histological and immunohistochemical examination. In the untreated diabetic group, the islets appeared shrunken with disturbed architecture and abnormal immunohistochemical reactions for insulin, caspase-3, and inducible nitric oxide synthase (iNOS). The biochemical findings were also disturbed. Morphometrically, there was a significant decrease in the islet size and islet number. Treatment with sitagliptin, metformin, and their combination showed an improvement, with the best response in the combined approach. No evidence of pancreatic injury was identified in the sitagliptin-treated groups. In conclusion, sitagliptin had a cytoprotective effect on beta-cell damage. Furthermore, the data didn’t indicate any detrimental effects of sitagliptin on the exocrine pancreas.

scholarly journals Pengaruh Asam Alfa Lipoat Terhadap Kadar Trigliserida pada Tikus Diabetes Melitus Tipe 2

2018 ◽  
Vol 12 (1) ◽  
pp. 13
Author(s):  
Angel Chen ◽  
Ismawati Ismawati ◽  
Enikarmila Asni
Keyword(s):  
Diabetes Mellitus ◽  
Insulin Resistance ◽  
Triglyceride Level ◽  
Diabetic Rats ◽  
Male Rats ◽  
Alpha Lipoic Acid ◽  
Type 2 Diabetic ◽  
Wistar Male Rats ◽  
Diabetes Melitus

Accumulation of triglycerides associated with  insulin resistance in type 2 diabetes mellitus (T2DM). Alpha lipoic acid (ALA) has hypotriglyceridemic effects by lowering triglyceride levels in blood and liver. This study aims to analyze effect of ALA on triglyceride levels in  type 2 diabetic rats. Fifteen Wistar male rats (±8-12 weeks, 200-250 gram) divided into 3 groups (control, T2DM  and  T2DM–ALA given for 3 weeks). Diabetes was induced by giving streptozotocin (50 mg/kg.bw) and nicotinamide (110 mg/kg.bw) after 15 minutes intraperitoneally. Diagnosis of T2DM was determined if the rats have perifer blood glucose >250 mg/dL after 3 days. The results showed that triglyceride levels in  ALA group (p=0,003) lower than T2DM group, T2DM group (p=0,031)  had higher triglyceride level than control and no difference of triglyceride level between  T2DM-ALA given group with control (p=0,696). This study concluded that ALA can affect triglyceride levels in T2DM thus prevents occurrence of hypertriglyceridemia.

scholarly journals Effect of Eight Weeks Resistance Training On Gene Expression of TNF-Α and IL10 in the Heart of Type II Diabetic Male Rats

2019 ◽  
Author(s):  
Zahra Soltanian ◽  
Behnaz Vanaky ◽  
Nasrin Ramezani ◽  
Nader Shakeri ◽  
Zahra Shams ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Resistance Training ◽  
Training Group ◽  
Heart Tissue ◽  
Diabetic Rats ◽  
Male Rats ◽  
Inflammatory Factors ◽  
Tnf Α ◽  
Type 2 Diabetic

Introduction: Type 2 diabetes has many complications, including cardiovascular disease, which is associated with an increase in inflammatory biomarkers. Research has shown that physical activity can reduce inflammatory factors and improve cardiovascular disease. The purpose of this study was to investigate the effect of resistance exercise on some of the inflammatory and anti-inflammatory factors in the heart tissue of type 2 diabetic rats. Methods: In this study, 18 male rats were selected. Rats were diabetic with nicotinamide and streptozotocin (stz) and then randomly divided into 2 groups of control (n=9) and training (n=9). The training group performed a resistance training program for 8 weeks, 5 days a week. Measurement of TNF-α and IL10 expression in the heart tissue was determined using the one step SYBR TAKARA single-step kit. To compare the difference between the mean of variables between exercise and control groups, independent t-test and t-test were used also to determine the effect of diabetes induction on variables. To determine the relationship between variables, Pearson correlation coefficient and separation correlation coefficient were used. Data were analyzed by SPSS software version 22. Results: The results showed that the insulin resistance index decreased significantly in the training group compared to the control group. On the other hand, the expression of TNF-α gene decreased and IL10 showed a significant increase (α≤0.05). Conclusion: The results indicate that resistance training may be effective in modulating the inflammatory factors of TNF-α and IL10 in the heart tissue of type 2 diabetic rats. Type 2 diabetes, Heart, Resistance training, Inflammatory factors.

Effect of nitrate on gene and protein expression of nitric oxide synthase enzymes in insulin-sensitive tissues of type 2 diabetic male rats

2021 ◽  
Vol 21 ◽  
Author(s):  
Majid Shokri ◽  
Sajad Jeddi ◽  
Hassan Faridnouri ◽  
Vajiheh Khorasani ◽  
Khosrow Kashfi ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Protein Expression ◽  
Mrna Level ◽  
Diabetic Rats ◽  
Metabolic Effects ◽  
Male Rats ◽  
Epididymal Adipose Tissue ◽  
Protein Levels ◽  
Gene And Protein Expression

Background and Objective: Decreased nitric oxide (NO) bioavailability contributes to the pathophysiology of type 2 diabetes mellitus (T2DM). This study aims to determine the effects of nitrate (NO3–) on gene and protein expression of NO synthase (NOS) enzymes in the liver, soleus muscle (SM), and epididymal adipose tissue (eAT) of rats with T2DM. Methods: Twenty-eight male rats were divided into 4 groups: Control, diabetes, control+NO3–, and diabetes+NO3– (n = 7/each group). NO3– was administered for 6 months, and mRNA and protein levels of NOS enzymes were measured at the end of the study. Results: mRNA and protein levels of inducible NOS (iNOS) were higher in the liver (475% and 73%), SM (271% and 43%), and eAT (543% and 24%) of rats with T2DM. In the case of the endothelial NOS (eNOS), diabetic rats had lower mRNA and protein levels in the liver (26% and 24%) and SM (60% and 62%) and lower mRNA level (30%) in eAT. mRNA and protein levels of neural NOS (nNOS) were lower in SM (69% and 73%) and eAT (25% and 31%) of rats with T2DM. NO3– administration restored disrupted iNOS and eNOS expressions to their near normal values in all the studied tissues; NO3– also increased nNOS mRNA and protein levels in SM and eAT but decreased nNOS protein level in the liver. Conclusion: Long-term NO3– administration restored disrupted expression of NOS enzymes in the liver, SM, and eAT of rats with T2DM; these findings partly explain the beneficial metabolic effects of nitrate in T2DM.

scholarly journals The Effect of 12 Weeks Aerobic Training on PDX-1 and GLUT2 Gene Expression in the Pancreatic Tissue of Type 2 Diabetic Rats

2020 ◽  
Author(s):  
Shokoufeh Sokhanvardastjerdi ◽  
Abdolali Banaeifar ◽  
Sajad Arshadi ◽  
Ardeshir Zafari
Keyword(s):  
Gene Expression ◽  
Aerobic Training ◽  
Serum Glucose ◽  
Pancreatic Tissue ◽  
Diabetic Rats ◽  
Male Rats ◽  
P Value ◽  
Glut2 Gene ◽  
Type 2 Diabetic

Objective: The aim of this study was to investigate the effect of 12 weeks aerobic training on PDX-1 and GLUT2 gene expression in the pancreatic tissue of type 2 diabetic rats. Materials and Methods: 21 wistar male rats were placed in 3 groups (healthy, diabetic, aerobic diabetic). Diabetes was induced by peritoneum injection of nicotine amid. Training program lasted 12 weeks, five sessions per week by gradual increase of speed (18 to 26 meters on minutes) and time (10 to 55 minutes) in the form of running on the treadmill. After 12 week aerobic training, PDX-1 and GLUT-2 genes were measured. Data was analyzed using ANOVA with 5 % significance level. Results: Aerobic training caused a significant increase in the expression of GLUT2 (P-value: 0.043) and PDX-1 genes (P-value: 0.007) in the pancreatic tissue of rats with type 2 diabetes (p value: 0.05). Also aerobic training had a significant effect on serum glucose (P-value: 0.001) and insulin levels (P-value: 0.001). Conclusion: It is concluded that aerobic training has significant effects on diabetic control by increasing the expression of PDX1 and GLUT-2 which lowers serum glucose.

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