scholarly journals The Number of Macrophages and Heterophils on Chick Embryo Chorioallantoic Membrane After Gynura procumbens (Lour) Merr Extract Treatment and bFgF Induction

2012 ◽  
Vol 3 (2) ◽  
pp. 398
Author(s):  
Iwan Sahrial Hamid ◽  
Yuseni Kusuma P. ◽  
Retno Bijanti ◽  
Bimo Aksono
Keyword(s):  
Chick Embryo ◽  
Chorioallantoic Membrane ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Group Vi ◽  
Antiangiogenic Effect ◽  
Treatment Groups ◽  
Group I ◽  
Chick Embryo Chorioallantoic Membrane

Antiangiogenesis (inhibition of new blood vessels formation) has become a strategy to inhibit cancer development. The aim of this experiment was to investigate antiangiogenic effect of Gynura procumbens (Lour) Merr focusing on the decreasing of the number of macrophages and heterophils on chick embryo chorioallantoic membrane. Nine-days-aged-eggs were divided into six groups (eight eggs each group). Group I (positive control) eggs were induced with bFGF+Tris HCl. Group II (negative control) eggs were treated with DMSO+Tris HCl. Group III (treatment I) eggs were induced with 60 ng bFGF and treated with ethanolic extract of G. procumbens leaves with the dose of 60 µg. The following treatment groups, i.e. group IV (treatment II), group V (treatment III), and group VI (treatment IV) were treated with increasing dose of extract, starting from 75 µg, 90 µg, and the last was 110 µg. Eggs were incubated until they reach the age of twelve days to observe macrophages, while to observe heterophils, eggs were incubated until the age of seventeen days. Based on haematoxylin-eosin staining, macrophages in the treatment groups were less than the control positive group (bFGF+Tris HCl), but based on giemsa staining, the effect of Gynura procumbens in decreasing the number of heterophils could not be observed because some blood smears. These analysis suggest that the ethanolic extract of Gynura procumbens leaves can perform as antiangiogenic agent decreasing the number of macrophages.Keywords: antiangiogenic, macrophages, heterophils, Gynura procumbens

scholarly journals HEPATOPROTECTIVE ACTIVITY COMBINATION OF CURANGA FEL-TERRAE LOUR LEAVES AND CURCUMA HEYNEANA VALETON AND ZIJP RHIZOME IN RAT INDUCED BY COMBINATION OF RIFAMPIN AND ISONIAZID

2017 ◽  
Vol 9 (5) ◽  
pp. 23
Author(s):  
Marianne . ◽  
Jessy Chrestella ◽  
Maria Andriani Ginting ◽  
Aminah Dalimunthe ◽  
Rosnani Nasution
Keyword(s):  
Normal Control ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Group Vi ◽  
Group V ◽  
Treatment Groups ◽  
Group I ◽  
Male Wistar Rats

Objective: The liver is a vital organ in the body, it is often exposured to the xenobiotics that can cause injury. Pugun tano (Curanga fel-terrae) and temu giring (Curcuma heyneana) are plants that have been claimed to cure many ailments including protecting the liver. But the hepatoprotective properties of the combination of these plants has not been well studied. In this regard, the current study was undertaken to evaluate the activity combination of those plants.Methods: The male Wistar rats were divided into 6 groups, group I was a negative control (CMC-Na); group II-IV were the treatment groups and were given combination extracts at the doses of 50 mg/kg, 75 mg/kg and 100 mg/kg respectively; group V was positive control (catechin) and group VI was normal control. All of groups except group VI were given combination rifampin 100 mg/kg and isoniazid 50 mg/kg for 15 d along with administration of extracts. At the day 16, rats were sacrificed. Histopathology of the liver and biochemical assay of blood was done at the end of the administration.Results: The administration of the combination of C. fel-terrae and C. heyneana at the doses of 75 and 100 mg/kg significantly inhibited the elevation of aspartate aminotransferase (AST) and alanine transaminase (ALT) compared to negative control (p<0.05). Histopathological assessment of the liver was comparable to the normal control (p>0.05).Conclusion: The combination of C. fel-terrae and C. heyneana at the dose of 75 and 100 mg/kg is able to protect the liver from damage induced by rifampin and isoniazid.

scholarly journals Comparative Evaluation of Apical Sealing Ability of Different Obturation Techniques by Confocal Microscopy: An In Vitro Study

2020 ◽  
Vol 8 (02) ◽  
pp. 55-59
Author(s):  
Reetu Arora ◽  
Yogesh Kumar ◽  
Neetu Jindal ◽  
Renu Aggarwal ◽  
Kavneet Takhar
Keyword(s):  
Root Canal ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Control Group ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Group I

Abstract Introduction The aim of obturation in the root canal is to completely seal the canal space to eliminate all the portals of entry and exit between root canal and periodontal space. Various techniques have been developed to achieve a hermetic seal. Materials and Methods As many as 150 extracted human maxillary central incisors were taken for the study. Biomechanical preparation was done up to F5 protaper file. According to different obturation techniques, samples were divided into six groups, keeping 30 samples in experimental and 15 samples in control groups. Group I–Lateral Condensation, Group II–Thermafil, Group III–Beefill, Group IV–GuttaFlow, Group V–Positive Control group, Group VI–Negative Control group. After obturation, the samples were immersed in 2% Rhodamine-B dye for 24 hours. Each sample was longitudinally sectioned to examine under confocal laser scanning microscope. Statistical Analysis The results were evaluated with ANOVA and posthoc Tukey honest significant difference (HSD) comparison test. Results The mean values of dye penetration of different groups were Group I (Lateral Condensation) 1.51 ± 0.451, Group II (Thermafil) 0.918 ± 0.399, Group III (Beefill) 1.30 ± 0.559. Group IV (GuttaFlow) 0.655 ± 0.396, Group V (Positive Control group) 1.96 ±0.046, Group VI (Negative Control group) 0 ± 0. The lowest mean value of apical microleakage was found in GuttaFlow amongst all experimental groups. Conclusion It can be concluded that the GuttaFlow obturating material exhibited better apical sealing ability with canal walls.

scholarly journals Immunomodulator Activity of Effervescent Granule of Wualae Fruit (Etlingera elatior (Jack) R.M. Smith) Based on Specific Phagocytic Activity

2019 ◽  
Vol 2 (2) ◽  
pp. 35-40 ◽  
Author(s):  
Adryan Fristiohady ◽  
Wa Ode Siti Zubaydah ◽  
Wahyuni Wahyuni ◽  
Mirda Mirda ◽  
Saripuddin Saripuddin ◽  
...  
Keyword(s):  
Phagocytic Activity ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Etlingera Elatior

The previous study reported that Etlingera elatior (Wualae) has activity as immunostimulant with dose at 300 mg/kg BW and 400 mg/kg BW. Formulating natural product into effervescent granule (GE) can increase practicality and interest to consume traditional medicine. This study aims to investigate the immunostimulant activity of Wualae ethanolic extract in the effervescent granule. Wualae was macerated with ethanol then formulated into effervescent granule at dose 300 mg/kgBW and 400 mg/kgBW. Formulations were evaluated. Animals were divided into 4 groups and treated with: group I (positive control); group II (negative control); group III (GE dose at 300mg/kgBW); and group IV (GE at dose 400mg/kgBW) for 7 days and at days 8, groups were infected by Staphylococcus aureus intraperitoneally. Immunostimulant activity was measured by calculating the Specific Phagocytic Activity (SPA) of macrophage. Data collected from the evaluation and measurement of the SPA then analyzed using SPSS. Results from the evaluation were considered good except the moisture content of effervescent granule. Results from SPA was found that effervescent granule at dose 300 mg/kgBW and at dose 400 mg/kgBW have 26.33% and 32.5% consecutively with significance 0.02 (p<0.05). Thus, effervescent granule has comparable phagocytosis activity with positive control.

scholarly journals UJI AKTIVITAS ANTIULCER AIR ALKALI TERIONISASI PADA MENCIT PUTIH JANTAN (MUS MUSCULUS)

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Adriyan Suhada ◽  
Bq. Fitria Maharani ◽  
Ni Komang Wijiani Yanti ◽  
Nur'aini Nur'aini
Keyword(s):  
Mus Musculus ◽  
Mucosal Damage ◽  
Positive Control ◽  
Negative Control ◽  
Gastric Mucosal Damage ◽  
Group Vi ◽  
Alkaline Water ◽  
Water Group ◽  
Group I ◽  
Water Ph

Alkaline water is water that has antioxidant content and has a structure that is rich in active hydrogen that can cure stomach ulcers. The purpose of this study was to determine the effectiveness of ionized alkaline water as an antiulcers in male white mice (mus musculus). Method of induction of gastric ulcer using aspirin, used as many as 24 male mice (mus musculus) aged 2-3 months used as mice test animals divided into 6 groups each group consisting of 3 animals all groups were given ulcer treatment for 7 days, then group I (negative control) were given aquadest group II (positive control) were given sucralfate, group III were given ph 8 alkaline water group IV was given ph 9.0 alkaline water, group V was given ph 9.5 alkaline water, and group VI was given alkaline water pH 11.5 treatment of each group for the next 7 days on the 14th day the mice were dissected and the gastric mucosa structure was observed and the mucosal damage value was determined. The results showed that alkaline water 9.5 and 11.5 had lower mucosal damage values than negative controls. It can be concluded that the alkaline water ph 9.5 and 11.5 are said to be effective in reducing gastric mucosal damage compared to the negative group given aquadest.

scholarly journals Analisis Metabolit Sekunder dan Aktivitas Ekstrak Etanol Kulit Buah Salak (Salacca zalacca (Gaertn.) Voss) Terhadap Kadar Glukosa dan Ureum Kreatinin Tikus Putih Jantan (Rattus norvegicus)

KOVALEN ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 161-168
Author(s):  
Tien Wahyu Handayani ◽  
Agustinus Widodo ◽  
Risna Yanti ◽  
Erdy Prasetyo ◽  
Zulfaidah ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Rattus Norvegicus ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Phytochemical Screening ◽  
Fruit Peel ◽  
Treatment Groups ◽  
White Rats

The snake fruit peel (Salacca zalacca (Gaertn.) Voss) traditionally has several health benefits, one of which is to treat diabetes mellitus. This study aimed to determine the content of secondary metabolites in the ethanolic extract of snake fruit peel and its activity on glucose and urea creatinine levels in male white rats (Rattus norvegicus). Phytochemical screening of extracts was carried out qualitatively and quantitatively using UV-Vis Spectrophotometry. This study is a laboratory experimental study using 30 test animals divided into 6 treatment groups (normal control, negative control, positive control, 70 mg/kg BW, 140 mg/kg BW, and 280 mg/kg BW). The results showed that the ethanol extract of the bark of the salak fruit contained 7.61 %w/w alkaloids, flavonoids 0.041% w/w, tannins 1.18% w/w, and saponins 2% w/w. Ethanol extract of salak fruit peel dose of 140 mg/kg BW affected decreasing blood glucose and urea creatinine levels. The skin of the salak fruit has the potential to be further investigated as an antidiabetic.

Pengujian Efektivitas Penyembuhan Luka Mencit Diabetes Melitus Yang Diberikan Sediaan Krim Ekstrak Etanol Daun Bandotan

2016 ◽  
Vol 6 (1) ◽  
pp. 50
Author(s):  
Ria Afrianti ◽  
Dedi Nofiandi ◽  
Dira Dira ◽  
Widya Ulfa
Keyword(s):  
Wound Healing ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Collagen Fibers ◽  
Group Iv ◽  
Ageratum Conyzoides ◽  
Group V ◽  
Group Iii ◽  
Group I

Bandotan plants (Ageratum conyzoides (L.) L.) commonly used as agent in the treatment of inflammatory, wound, and also used used as analgesic, stimulant and tonic. In this study we carried out a test of the effectiveness of the cream (w/o) of ethanolic extract of bandotan leaves in wound healing in streptozocin-induced hyperglycemia mice. Animals were divided into five groups: Group I (without induced, given cream base w/o), group II (induced, given cream base w/o), group III (induced, given cream containing 3% extract), group IV (induced, given cream containing 5% extract) and group V (induced, given cream containing 7% extract). Animals also divided into three subgroups based on time of decapitation on 7th, 14th and 21th days. The parameters observed were the percentage of wound healing and collagen fibers density scores. Results were analyzed by Two-Way ANOVA. The results showed that the cream in concentration of 3%, 5% and 7% could affect wound healing in hyperglycemia mice. The percentage of wound healing of the cream in concentration of 3%, 5% and 7% were significantly different (p<0,05) with positive control, and density scores of collagen fibers was also significantly different (p<0,05) from the positive control and negative control. However, the best results was obtained from cream containing 5% extract which perrcentage of wound healing reached 98.5% on 21th day and got a score of 3 for density of collagen fibers that had been formed on the 14th day. 

scholarly journals Uji Efek Stimulansia Ekstrak Etil Asetat Kulit Kayu Akway (Drymis piperita) Asal Papua Pada Tikus (Ratus norvegicus) Jantan

2020 ◽  
Vol 12 (1) ◽  
pp. 37-42
Author(s):  
Rani D. Pratiwi ◽  
Eva S. Simaremare
Keyword(s):  
Body Weight ◽  
Treatment Group ◽  
Research Result ◽  
Stem Bark ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Group Vi ◽  
Group I ◽  
Significant Difference

Drymis sp. stem bark from Papua widely utilized by local people especially in Sougb Tribe from Sururey Distric Pegunungan Arfak West Papua, one of them is used to increase the stamina of the body. The aim of this study was to investigate the tonic effect of Drymis sp. stem bark of Ratus norvegicus. Ratatory exhaustion method was used to the tonic effect. Selected rats (30) were divided into 6 groups, each consisting of 5 rats. Tested extract was administrated oraly one dose to the rats. Group I, II and III was given the ethil acetat fraction of Drymis sp. stem bark 10mg/kg body weight, 30mg/kg body weight and 50mg/kg body weight consecutively. Group IV was given CMC-Na 0,5% (as negative control). Goup V was given caffein 15 mg/kg body weight (as positive control) and Group VI as a control. The total swimming period until exhaustion was measured and use as index of swimming capacity. Research result show that the highest dose 50 mg/body weight of the tested fraction the longer swimming time and with better stamina. The result of One Way ANOVA test showed that there was a significant difference between treatment group, followed by tukey test, tukey result obtained the ethil acetat fraction and negative control (CMC Na) showed that there was significant difference, and there is no real difference between between treatment group and positive control.   Key words: Drymis sp, Papua, tonic effect, rats.   

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