scholarly journals Different 4T1 Cells Migration under Caesalpinia sappan L. and Ficus septica Burm.f Ethanolic Extracts

2017 ◽  
Vol 8 (1) ◽  
pp. 21 ◽  
Author(s):  
Sari Haryanti ◽  
Retno Murwanti ◽  
Herwandhani Putri ◽  
Gagas Pradani Nur Ilmawati ◽  
Suwijiyo Pramono ◽  
...  
Keyword(s):  
Anticancer Agent ◽  
Gelatin Zymography ◽  
Ethanolic Extract ◽  
Cell Types ◽  
Inhibitory Effect ◽  
Caesalpinia Sappan ◽  
Ic50 Value ◽  
Anti Cancer ◽  
Ethanolic Extracts ◽  
4T1 Cells

Secang (C. sappan L.) and awar (F. septica Burm.f) are known of Indonesian traditional medicine that highly consumed throughout centuries in order to cure various diseases. Recently, researchers also concern about its effects as anti-cancer on various cell types. This study was conducted to understand the ethanolic extract of C. sappan L. (ECS) and F. septica Burm.f (EFS) effects on 4T1 cells migration at various concentrations. Firstly, we examine cell proliferation profile with MTT assay under treatment with the extracts and obtained the IC50 value of ECS (20 μg/mL) and EFS (15 μg/mL). Subsequent assay conducted was to examine the cells migration under low concentration resulting in the migration inhibitory effect of both EFS and ECS with different intensity.  EFS performed better migration inhibitory effect than ECS. Finally, we conducted gelatin zymography and western blot and revealed that the migration inhibitory effect of EFS may correlate to the lowering of protein expression of MMP9 and Rac-1 after 24 hours of treatment. We conclude that both extracts are potential to be developed as anticancer agent and EFS is more potent for anti-metastasis.Keywords: C. sappan L., F. septica Burm.f, 4T1, anti-migration

Evaluation of Phytochemicals and Pharmacological Activities of Ben-Cha-Lo-Ka-Wi-Chian Remedy

2019 ◽  
Vol 891 ◽  
pp. 52-59
Author(s):  
Chanai Noysang ◽  
Teerarat Pummarin
Keyword(s):  
Antioxidant Activity ◽  
Minimum Inhibitory Concentration ◽  
Inhibitory Concentration ◽  
Propionibacterium Acnes ◽  
Ethanolic Extract ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Pharmacological Activities ◽  
Ethanolic Extracts ◽  
Reaction Test

Benchalokawichian (BLW) remedy is a Thai traditional medicine that has been notified in the List of Medicine Products of the National List of Essential Drugs A.D. 2006 and has long been used as an antipyretic. The phytochemicals of the 70% and 95% ethanolic extracts of were studied by color reaction test and HPTLC analysis. The BLW remedy showed a highest amounts of 70% ethanolic extractives (ca. 3.99 ± 0.60% of dry material weight). The several ethanolic extracts showed similar qualitative phytochemicals. The major of phytochemicals identified in these extracts were alkaloids, flavonoids, tannins, and polyphenols. The antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl assay. The analysed 70% ethanolic extract showed a highest antioxidant activity at IC50= 526.09 µg/ml and both extracts not showed tyrosinase inhibitory effect. The 95% ethanolic extract showed the greatest anti-propionibacterium acnes activity with inhibition zone of 10.10 ± 0.45 mm and minimum inhibitory concentration (MIC) was 5.05 mg/ml

Preliminary Phytochemicals and Pharmacologic Activities Assessment of White and Pink Nelumbo nucifera Gaertn. Flowers

2019 ◽  
Vol 891 ◽  
pp. 41-51
Author(s):  
Chanai Noysang ◽  
Nutsuda Boonmatit
Keyword(s):  
Antioxidant Activity ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
White Flower ◽  
Nelumbo Nucifera ◽  
Color Reaction ◽  
Natural Source ◽  
Pink Flower ◽  
Ethanolic Extracts ◽  
Reaction Test

The phytochemicals of the ethanolic extracts of petals and stamens of lotus flowers from several lotus cultivars belonging to the Nelumbo nucifera Gaertn. (namely 'lotus white flower' and 'lotus pink flower') were studied by color reaction test and HPTLC analysis. The lotus pink flower stamens showed a highest amount of ethanolic extractives (ca. 18.33±2.14% of dry material weight). The several the ethanolic extracts of N. nucifera petals and stamens showed similar qualitative phytochemicals. The crude extracts revealed the presence of flavonoids, terpenoinds, tannins and polyphenols. The antioxidant activity was assessed using the 2,2-diphenyl-1-picrylhydrazyl assay. The analyzed lotus pink flower stamens ethanolic extract showed a highest antioxidant activity at IC50 = 0.61±0.16 μg/ml and lotus pink flower petals ethanolic extract showed a highest tyrosinase inhibitory effect at IC50 = 2.25±0.21 μg/ml. N. nucifera flowers could be provide a potential natural source of phytochemicals and could be beneficial to the cosmetic properties.

scholarly journals Radical Scavenging Activity Combination of Sambiloto (Andrographis paniculata Nees.) and Patikan Kebo (Euphorbia hirta L.) Ethanolic Extracts on 2,2-Diphenyl-1-Picrylhidrazyl (DPPH)

2018 ◽  
Vol 23 (3) ◽  
pp. 79
Author(s):  
Andayana Puspitasari Gani ◽  
Suwidjiyo Pramono ◽  
Sudibyo Martono ◽  
Sitarina Widyarini
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Andrographis Paniculata ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Euphorbia Hirta ◽  
Ic50 Value ◽  
Ethanolic Extracts ◽  
Dpph Radical Scavenging

Research of radical scavenging activity of sambiloto (Andrographis paniculata Nees) and patikan kebo (Euphorbia hirta L) ethanolic extracts and their combination toward 2,2-Diphenyl-1-Picrylhidrazyl (DPPH) has conducted. Subjects of this experiment comprise sambiloto ethanolic extract, patikan kebo ethanolic extract, dechlorophyllated extract of sambiloto and combinations among those three. Dechlorophyllation by electrocoagulation method is done to reduce chlorophyll content thereby increasing the level of active compound in the sambiloto extract. The results of dechlorophyllation showed that only total flavonoid content in the extract of sambiloto increased, meanwhile the radical scavenging activity results showed that patikan kebo ethanolic extract has the lowest IC50 value (22,36 µg/mL) compared to sambiloto ethanolic extract (IC50 value 499,03 µg/mL). Extract combination may increase DPPH radical scavenging activity of the sambiloto extract (IC50 value 76,06 µg/mL) but not higher than that of patikan kebo single extract.

scholarly journals Ethanolic Extract of Hedyotis corymbosa L. Inhibits Migration and MMP-9 Activity on Metastatic Breast Cancer Cells

2018 ◽  
Vol 9 (1) ◽  
pp. 16
Author(s):  
Dhania Novitasari ◽  
Sri Handayani ◽  
Riris Istighfari Jenie
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Metastatic Breast ◽  
Gelatin Zymography ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Anticancer Properties ◽  
Scratch Wound ◽  
4T1 Breast Cancer

Many natural products have been widely explored for their pharmacological activities, including anticancer activity. Hedyotis corymbosa L. is known for its anticancer properties toward several cancer cell lines. The study aims to investigate whether ethanolic extract of Hedyotis corymbosa L. (EEH) performs anticancer properties by inhibiting migration and metastasis on breast cancer cells. Cytotoxic evaluation by using MTT assay was carried out to determine EEH effect on 4T1 breast cancer cells, meanwhile to investigate the treatment of EEH in migration and metastasis inhibitory effect, scratch wound healing assay and gelatin zymography were conducted in this study. The data showed that EEH possessed cytotoxic activity with IC50 value of 400 μg/mL.  Interestingly, migration inhibitory effect was shown up to 42 hours and the activity of MMP-9 was also decreased after the treatment with EEH. According to these findings, we suggest that Hedyotis corymbosa L. promotes another anticancer properties by inhibiting migration and metastasis towards breast cancer cells.Keywords : Hedyotis corymbosa L., cytotoxicity, migration, metastatic, 4T1 breast cancer cells

scholarly journals Heartwood of Secang (CAESALPINIA SAPPAN L.) Ethanolic Extract Show Selective Cytotoxic Activities on T47D and Widr Cells But Not on Hela Cells

2017 ◽  
Vol 7 (2) ◽  
pp. 60
Author(s):  
Erlina Rivanti ◽  
Bani Adlina Shabrina ◽  
Ika Nurzijah ◽  
Cyndwika Ayu ◽  
Adam Hermawan
Keyword(s):  
Cancer Cells ◽  
Hela Cells ◽  
Breast Cancer Cells ◽  
Ethanolic Extract ◽  
Vero Cells ◽  
Cytotoxic Activities ◽  
Colon Cancer Cells ◽  
Caesalpinia Sappan ◽  
Ic50 Value ◽  
Cervical Cancer Cells

The present study investigate the selectivity of heartwood of secang ethanolic extract (SEE) on T47D breast cancer cells, WiDr colon cancer cells, and HeLa cervical cancer cells, compared to Vero normal epithelial cells. The cytotoxic effect was evaluated by using MTT assay  with 24-hour treatment to get IC50values. Selectivity was evaluated by using selectivity index (SI). SEE had a potent cytotoxic activity on T47D and WiDr cancer cells (IC50 <100 µg/ml). IC50 value of HeLa cancer cells was observed on moderate cytotoxic (100 <IC50 <1000 µg/ml). SEE demonstrated more selective to T47D and WiDr than Vero cells (SI > 3), while in HeLa cells is not selective (SI < 3). This result indicating its potential of Caesalpinia sappan as a chemopreventive agent in cancer therapy.Keywords: Cancer, selectivity, Secang, T47D, WiDr, HeLa, Vero

scholarly journals Antimigratory Activity of Brazilin-Containing Fraction from Caesalpinia sappan L. on MDAMB-231 Cells

2020 ◽  
Vol 27 (4) ◽  
pp. 266
Author(s):  
Sri Handayani ◽  
Ratna Asmah Susidarti ◽  
Puspa Dewi Narrij Lotulung ◽  
Akhmad Darmawan ◽  
Edy Meiyanto ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Combination Index ◽  
Synergistic Effects ◽  
Biological Activities ◽  
Gelatin Zymography ◽  
Inhibitory Effect ◽  
Cytotoxic Effects ◽  
Caesalpinia Sappan ◽  
Liquid Liquid Extraction ◽  
Synergistic Inhibitory Effect

Caesalpinia sappan is studied for several biological activities. The aim of this research is to determine the cytotoxic and antimigratory activities of Caesalpinia sappan active fraction in combination with cisplatin on human TNBC cells (MDA-MB-231). Caesalpinia sappan heartwood was extracted with methanol. Then, several fractions of the methanol extract were obtained by using a liquid-liquid extraction method followed by column chromatography. The cytotoxicity was determined using MTT assay. Synergistic effects were analyzed by calculating the combination index (CI). Migration was examined using wound-healing assay. Levels of MMP2 activity were determined with gelatin zymography assay. The results showed that most of the fractions included in this study exhibited cytotoxic effects against MDA-MB-231 cells, and C fraction demonstrated the highest cytotoxic activity of all fractions. The combination of C-cisplatin revealed a synergistic inhibitory effect on MDA-MB-231 cell growth (CI<1). Furthermore, C fraction, alone and in combination with cisplatin, inhibited migration of MDA-MB-231 and suppressed MMP2 activity. The C fraction isolated from Caesalpinia sappan increased the cytotoxic and antimigratory activities of cisplatin on MDA-MB-231 cells. Based on these findings, the potential of Caesalpinia sappan to act as a supportive agent in metastatic TNBC treatment with cisplatin warrants further exploration.

scholarly journals Ethanolic Extract of Secang (Caesalpinia sappan L.) Wood Performs as Chemosensitizing Agent Through Apoptotic Induction on Breast Cancer MCF-7 Cells

2012 ◽  
Vol 3 (3) ◽  
pp. 444 ◽  
Author(s):  
Rahmi Khamsita ◽  
Adam Hermawan ◽  
Dyaningtyas Dewi Pamungkas Putri ◽  
Edy Meiyanto
Keyword(s):  
Breast Cancer ◽  
Annexin V ◽  
Ethanolic Extract ◽  
Caesalpinia Sappan ◽  
Ic50 Value ◽  
Dose Dependent ◽  
Resistance To Chemotherapy ◽  
Apoptotic Induction ◽  
Combinatorial Treatment ◽  
Mcf 7

Resistance to chemotherapy is believed to cause treatment failure of the patient cancer. Secang (Caesalpinia sappan L.) has been proven to possess anticancer activity on some cancer cell lines. The aimed of this study to develop ethanolic extract of secang wood (EES) as chemosensitizing agent through apoptotic induction on breast cancer MCF-7 cells. Extraction of secang was done by using maceration with 70 % ethanol. Single and combinatorial treatment of EES and doxorubicin on MCF-7 breast cancer cells were analyzed by using MTT assay to determine the IC50 value and combination index (CI) to evaluate the combinatorial effect. Apoptosis was analyzed with flowcytometry (annexin V).  EES showed a dose-dependent cytotoxicity (IC50 value of 37 µg/ml), while combinatorial treatment showed that 7 concentrations was found to be synergist with doxorubicin on MCF-7 cells. Combinatorial treatment also triggered apoptotic instead of single treatment. Based on this result, we conclude that ethanolic extract of secang wood is potential as chemosensitizing agent in breast cancer.Keywords: Caesalpinia sappan L, MCF-7 cells, doxorubicin, apoptosis.  

scholarly journals Brazilein in Combination with Cisplatin Inhibit Proliferation and Migration on Highly Metastatic Cancer Cells, 4T1

2017 ◽  
Vol 21 (1) ◽  
pp. 38 ◽  
Author(s):  
Sri Handayani ◽  
Ratna Asmah Susidarti ◽  
Zalinar Udin ◽  
Edy Meiyanto ◽  
Riris Istighfari Jenie
Keyword(s):  
Cancer Cells ◽  
Cell Cycle Progression ◽  
Metastatic Cancer ◽  
Gelatin Zymography ◽  
Inhibitory Effect ◽  
Cycle Progression ◽  
4T1 Cells ◽  
4T1 Breast Cancer ◽  
And Migration ◽  
Induced Apoptosis

Brazilein performs anti­cancer activities on several cancer cells and potentially inhibits metastasis. The aims of this study is to observe the synergistic cytotoxic and migration inhibitory effect of brazilein combined with cisplatin on 4T1 breast cancer cells. Under MTT assay, we found that brazilein revealed cytotoxic effect on 4T1 cells in a dose­dependent manner (IC50=50 ± 0.3 µM). Combination of brazilein and cisplatin showed synergistic effect (CI=0.72). Flowcytometry analysis on the cell cycle progression showed that single treatment of 25 µM brazilein induced G2/M­phase accumulation, 12.5 µM cisplatin induced S­phase accumulation, while combination of brazilein and cisplatin induced S­phase and G2/Mphase accumulation. Combination of brazilein and cisplatin induced apoptosis higher than that of the single treatments. Based on wound healing assay, 12.5 µM brazilein and its combination with 6.25 µM cisplatin inhibited cells migration. Immunoblotting and gelatin zymography analysis showed that combination of brazilein and cisplatin inhibited the expression level of Rac1 and MMP9 proteins. Based on these results, we conclude that brazilein enhanced cytotoxic activity of cisplatin and inhibited migration on 4T1 cells and potentially can be developed as an enhancing cytotoxic and antimetastasis agent.

scholarly journals In vitro antimalarial activity assay of Ashitaba Leaf ethanolic extract (Angelica keiskei)

2021 ◽  
pp. 27-30
Author(s):  
Alvi Kusuma Wardani ◽  
Abdul Rahman Wahid ◽  
Miftahul Jannah
Keyword(s):  
Antimalarial Activity ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Positive Control ◽  
New Drugs ◽  
Test Results ◽  
Ic50 Value ◽  
Angelica Keiskei ◽  
Very High

Introduction: The incidence of malaria is still very high in number in the world. Difficulty in treating malaria is caused by the resistance of malaria parasites to conventional drugs. An alternative treatment that can be used to treat malaria is to discover new drugs from natural ingredients. Aim: This study aimed to determine the activity of the Ashitaba leaf ethanolic extract as an antimalarial drug to Plasmodium falciparum strain 3D7. Methods: This study tested the activity of Ashitaba extract on the growth of P.falciparum in five concentrations, namely concentration of 0.01 ppm, 0.1 ppm, 1 ppm, 10 ppm, and 100 ppm. Results: The test results showed that the highest inhibitory effect was found on the concentration of 100 ppm with percent inhibition of 79.47 ± 26.91%. The 50% inhibition to parasites showed the half maximal inhibitory concentration (IC50) value of 2.09 ppm, compared to the positive control of which the IC50 of chloroquine was 0.007 ppm. Conclusion: Ashitaba leaf extract can be considered to have very active anti-malarial activity, because it has an IC50 value of less than 5 ppm.

scholarly journals Effect of Serjania lethalis ethanolic extract on weed control

2013 ◽  
Vol 31 (2) ◽  
pp. 239-248 ◽  
Author(s):  
P.U. Grisi ◽  
S.C.J. Gualtieri ◽  
S. Anese ◽  
V.C. Pereira ◽  
M.R. Forim
Keyword(s):  
Seedling Growth ◽  
Weed Management ◽  
Plant Material ◽  
Ethanolic Extract ◽  
Inhibitory Effect ◽  
Dependent Manner ◽  
Buffer Solution ◽  
Concentration Dependent Manner ◽  
Promising Alternative ◽  
Ethanolic Extracts

The objective of this study was to evaluate the effect of the ethanolic extract of Serjania lethalis leaves and stems on the diaspore germination and seedling growth of wild poinsettia (Euphorbia heterophylla) and barnyardgrass (Echinochloa crus-galli). The crude ethanolic extract was prepared from 100 g of dry plant material dissolved in 500 ml of ethanol. The extracts were solubilized in a buffer solution containing dimethyl sulfoxide (DMSO) at concentrations of 10.0, 7.5, 5.0 and 2.5 mg mL-1. The effect of these extracts was compared with herbicide oxyfluorfen in bioassays. The ethanolic extracts of S. lethalis leaves and stems inhibited the germination and seedling growth of barnyardgrass and wild poinsettia in a concentration-dependent manner. The reduction in the root length of E. heterophylla seedlings might be attributed to the reduced elongation of metaxylem cells. The phytotoxicity of the extracts ranged according to the receptor species, and for some variables, the inhibitory effect was similar, and even superior, to that of the commercial herbicide. Thus, S. lethalis extracts might be a promising alternative for sustainable weed management.

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