scholarly journals Manganese (Mn) and Lead (Pb) Content in Lemna Culture Fertilized by Bioslurry

2020 ◽  
pp. 1-6
Author(s):  
Iskandar . ◽  
Yuli Andriani ◽  
Irfan Zidni
Keyword(s):  
Experimental Method ◽  
Culture Medium ◽  
Culture Media ◽  
Lemna Minor ◽  
Small Water ◽  
Randomized Design ◽  
Water Plant ◽  
Mn Content ◽  
Completely Randomized Design ◽  
Experimental Pond

Lemna minor is a small water plant that floats on water and has the potential to be fresh feed for fish. Bioslurry is a by-product of the management of biogas from livestock manure which is often used as fertilizer for plants because it contains nutrients in a readily available form. Among the contents contained in bioslurry, manganese and lead are important things related to their use for plants and fish. This study aims to determine the mineral (Mn) and lead (Pb) content in the medium of lemna cultivation which is fertilized with various doses of bioslurry. The study was conducted at the Ciparanje Experimental Pond, Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran, Indonesia. This study used an experimental method with Completely Randomized Design (CRD) with four treatments with three replications. The treatment in the form of adding bioslurry to the lemna culture media was 0.00%, 0.25%, 1.00% and 2.50% of the volume of water. The minor density of Lemna used is 800 g/m2. The culture was carried out in 4 cycles for 7 days. The parameters observed were Mn and Pb content in the lemna culture medium shortly after the addition of Bioslurry and after 7 days of lemna cultivation. Based on the results of the study, the use of 2.5% bioslurry concentration can produce the highest Mn content with a range of 2.0-4.6 ppm and 2.92 ppm Pb content after 7 days of lemna cultivation. In addition, the use of 2.5% bioslurry concentration resulted in Mn and Pb values in the culture medium shortly after stirring bioslurry in the lemna culture media. Based on the research results, the treatment of adding 2.5% bioslurry to the lemna culture media is the best dose for produced Mn for fish, as well as the safest Pb for aquaculture activities.

Pertumbuhan Dan Perkembangan Anggrek Dendrobium anosmum Pada Media Kultur In Vitro Dengan Beberapa Konsentrasi Air Kelapa

Agrologia ◽  
2018 ◽  
Vol 1 (1) ◽  
Author(s):  
S. Tuhuteru ◽  
Meity L Hehanussa ◽  
Simon H.T Raharjo
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Water Concentration ◽  
Medium Composition ◽  
Coconut Water ◽  
Orchid Species ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

Dendrobium anosmum is one of natural orchids in Indonesia. Optimization of medium composition for orchid propagation through in vitro culture is necessary to enhance propagule multiplication capabilities and quality. This study was aimed to study the influence of concentration of coconut water in culture medium on in vitro growth and development of D. anosmum orchid species and to determine the optimal coconut water concentration in culture media.  The experiment were arranged in a Completely Randomized Design with four treatments and eight replications. The treatments consisted of the addition of coconut water with concentrations: 0 ml•l -1 (control), 50 ml•l-1, 100 ml•l-1 and 150 ml•l-1. The results showed that addition of coconut water in culture medium gave different effect on shoot growth and multiplication of D. anosmum orchids.  Coconut water concentration of 100 ml•l-1 was the best concentration for growth and multiplication of D. anosmum orchids, based on both shoots and roots growth, plantlet height and wet weight.

scholarly journals Domestication of Red Seaweed (Gelidium latifolium) in Different Culture Media

2020 ◽  
Vol 25 (1) ◽  
pp. 39-44
Author(s):  
Andri Wijayanto ◽  
Ita Widowati ◽  
Tjahjo Winanto
Keyword(s):  
Research Laboratory ◽  
Culture Medium ◽  
Culture Media ◽  
Raw Material ◽  
Growth Media ◽  
Red Seaweed ◽  
Randomized Design ◽  
Gelidium Latifolium ◽  
Domestication Process ◽  
Completely Randomized Design

Gelidium latifolium is one of red seaweed types potentially can be developed as an industrial raw material. Since Gelidium is currently taken from ocean, the availability of seaweed from aquaculture is necessary to overcome the small number of its availability in nature. In Indonesia, G. latifolium cultivation has not been carried out so that domestication is required. The use of macro and micro nutrients in growth media is essentially needed for the domestication process. Domestication requires fast media and place for growth. The purpose of this study is to determine the growth of biomass and the survival of G. latifolium in different culture media. The study was conducted in a semi-outdoor research laboratory. The method used in this research is laboratory experimental method and Completely Randomized Design (CRD) with the treatment applied using 3 types of culture media (Urea: Za: TSP) by comparison (A) 100: 50: 50% (2 g.L-1), (B) 75: 75: 50% (2 g.L-1) and (C) 75: 50: 75% (2 g.L-1), with 3 replications. The seaweed was kept in 10 L of water in aeration equipped aquarium and filled with 10 g of G. latifolium on each treatment. The best growth rate of G. latifolium biomass is 5.67± 0.58 g and 100±0% are survived in C culture medium with a concentration of 75% Urea: 50% ZA: 75% TSP (2 g.L-1).

INFLUENCE OF CULTURE MEDIUM ON THE SPORULATION AND VIABILITY OF ASPERGILLUS SPP. AND TALAROMYCES SPP. ENTOMOPATHOGENIC FUNGI

2018 ◽  
Vol 18 (1) ◽  
pp. 12
Author(s):  
Yuyun Fitriana ◽  
Radix Suharjo ◽  
I Gede Swibawa ◽  
Purnomo . ◽  
Puji Lestari ◽  
...  
Keyword(s):  
Entomopathogenic Fungi ◽  
Culture Medium ◽  
Culture Media ◽  
Spore Production ◽  
Corn Meal ◽  
Spore Viability ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Completely Randomized Design

Influence of Culture Medium on the Sporulation and Viability of Aspergillus spp. and Talaromyces spp. Entomopathogenic Fungi. The purpose of this study was to determine the effect of three kinds of cultures media on the spore production and viability of Aspergillus spp. (AS1, 6, 7, 9) and Talaromyces spp. (AS2–5, 8, 10) entomopathogenic fungi. This study was arranged using Factorial-Completely Randomized Design (CRD) with 2 factors and 3 replications. The first factor was three kinds of cultures media (potato dextrose agar (PDA), corn meal agar (CMA), and sabouraud dextrose agar (SDA)) and the second one was isolates of Aspergillus spp. Or Talaromyces spp.. Data of spore production and spore viability were tested using ANOVA and if there was significantly difference, the data then further analyzed using Tukey‘s Honestly Significant Difference (HSD) test at 5% of significant level. The spore production of Aspergillus spp. were in the range of 0.58 - 14.27 x 108 spores mL-1 (PDA); 0.28 – 2.68 x 108 spores mL-1 (SDA) and 1.85 - 5.33 x 108 spores mL-1 (CMA). The highest spore production was achieved by AS1 isolate that was grown on PDA media. The spore produced by Talaromyces spp. were in the range of 2.15 – 28.62 x108 spores mL-1 (PDA); 0.28 – 29.43 x108 spores mL-1 (SDA); and 1.88 – 16.63 x108 spores mL-1 (CMA). The highest spore production was produced by AS8 isolate which were cultured on PDA. The spore viability among isolates of the two entomopathogenic fungi were not significantly different. The spore viability of Aspergillus spp. was in the range of 95.10 – 97.66% (PDA), 94.02 – 98.45% (SDA) and 92.86 – 98.20% (CMA). The spore viability of Talaromyces spp. was in the range of 95.83 – 100% (PDA), 85.83 – 100% (SDA), and 90.75 – 100% (CMA). Culture medium influenced spore production but not the spore viability. The best culture media used for spore production of both of the entomopathogenic fungi was PDA media.

scholarly journals PENGARUH PENCAMPURAN KOTORAN TERNAK SEBAGAI MEDIA KULTUR TERHADAP PERTAMBAHAN POPULASI CACING TANAH (Lumbricus rubellus)

2019 ◽  
Vol 34 (1) ◽  
pp. 75-80
Author(s):  
Satria Edi Putra ◽  
Iskandar Johan ◽  
Muhammad Hasby
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Fish Farming ◽  
Lumbricus Rubellus ◽  
Cattle Dung ◽  
Cow Dung ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Buffalo Dung ◽  
Growing Population

This research aims to determine the effect of mixing cattle dung as a culture medium to the growing population of earthworms. The research was conducted for 42 days starting from 18 October to 28 November 2017 at the fish farming school hall of Agriculture Faculty of Islamic University Riau Pekanbaru. The worm tree used in this study was as many as 960 tail or 80 tail for each container. Worms are maintained for 42 days with different mixtures of medium, i,e, 100% P1 dung treatment, P2 cow-dung treatment 50% + 50% buffalo dung, 50% cow dung + 50% goat dung, 50% cow dung + 50% chicken droppings. The research design was Completely Randomized Design with 4 treatments and 3 replications. The result of this research showed that the increase of population of earthworm with the mixing of cattle dung as culture media which conducted for 42 days is the best treatment of P2 as much as 4266 head/container, then the second one used was in treatment 3 as much as 1855 head/container, and the lowest increase of the population was at treatment P1 as much as 403 head/container, and followed by treatment P4 as much as 575 head/ container.

Pertumbuhan Cladocera jenis Chydoridae pada media kultur yang berbeda

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Denovis Sambode ◽  
Henneke Pangkey ◽  
Sartje Lantu
Keyword(s):  
Population Growth ◽  
Culture Medium ◽  
Quality Parameters ◽  
Organic Fertilizers ◽  
Cow Dung ◽  
Randomized Design ◽  
Period Data ◽  
Growth Development ◽  
Completely Randomized Design ◽  
Medium Culture

The aim of this study was to find out the effect of different organic fertilizers on the population growth of Chydorus sp. This research was conducted at Laboratorium of Nutrition and Food Technology, Faculty of Fishery and Marine Sciences, Sam Ratulangi University, from December, 2012 to January, 2013. The experiment was run in the Completely Randomized Design with 3 treatments and 3 replication. Chydorus sp. was cultured in 9 glass jars with a volume of 1 L each. Three treatments applied in this research included A: medium culture composed of 500ml of water and 50 grams of soil; B: medium culture composed of 500ml of water, 50 grams of soil and 10g of cow dung; C: medium culture composed of 500ml of water, 50 grams of soil and 10g of horse dung. The density of Cydorus sp. in each media was 10 individuals /500 ml water. Observation on the population growth, development of live preys, and water quality parameters was conducted for 20 days period. Data were statistically analized with Analysis of variance. The results showed the highest density of Chydorus sp. was reached by Chydorus cultured in medium with horse manure (2169 individual/500 ml), followed by medium culture with cow manure (1715 individual/500 ml), and the lowest in medium culture with soil which was 1065,33 individual/500 ml. However, Analysis of variances showed that Chydorus population growth were not significantly affected by different culture medium. Keywords: manure, liquid fertilizer, growth, Chydorus sp.

scholarly journals Sensitivity Testing of Ciplukan (Physalis angulata) Rod Crude Extract on The Bacterium Pseudomonas fluorescens In Vitro

2019 ◽  
Vol 10 (1) ◽  
pp. 24-30
Author(s):  
Ion Tarsardo Sianturi ◽  
Arief Prajitno ◽  
Ellana Sanoesi
Keyword(s):  
Experimental Method ◽  
Crude Extract ◽  
Inhibition Zone ◽  
Natural Material ◽  
Sensitivity Testing ◽  
Randomized Design ◽  
Physalis Angulata ◽  
Completely Randomized Design

Diseases is a problem in aquaculture, one of which is the diseases caused by the bacteriumP.fluorescens. Antibiotics and chemicals materials themselves can give the bacterium resistance and cause harm for the environment. One alternative that can be done is using a natural material, namely the rod of ciplukan (p. angulata). The purpose of this research was to explain the effect of P. angulata on the growth of p. fluorescens. The method which can be use is experimental method by using the research design of completely randomized design (ral) with 5 treatment and 2 control with 3 repetitions. The results showed that extract of P. angulata at various dose (6,67 ppt, 13,33 ppt, 19,99 ppt, 26,66 ppt and 33,33 ppt) exhibited anti-P. fluorescens activity with inhibition zone diameters in the range of (4,06±0,07 mm - 9,63±1,61 mm). Increashing extract dose lead to increased the inhibition zone. The extract dose of 33,33 ppt exhibited best anti-P. fluorescens activity in this research. The research results show that P. angulata is proven to be able to inhibit the P. fluorescens, but to prove the effectiveness of this material, an in vivo is required.

scholarly journals UTILIZATION OF PAPAYA LEAVES (Carica papaya L.) TO CONTROL ONION PEST SPODOPTERA EXIGUA (LEPIDOPTERA: NOCTUIDAE)LEPIDOPTERA (NOCTUIDAE)

2020 ◽  
Vol 7 (1) ◽  
pp. 1-7
Author(s):  
Muhammad Agung Permadi ◽  
Rafiqah Amanda Lubis ◽  
Syawaludin Syawaludin ◽  
Nova Suryani Pasaribu
Keyword(s):  
Experimental Method ◽  
Carica Papaya ◽  
Spodoptera Exigua ◽  
Toxic Compounds ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Lepidoptera Noctuidae

Spodoptera exigua is a migrant pest that attacks onions simultaneously in groups. The utilization of papaya leaves to control pests due to papaya leaves have toxic compounds that can kill pests. This study aims to measure the ability of papaya leaves to control S. exigua larvae. This research was conducted using a completely randomized design (CRD) experimental method with two treatment factors. The first factor was papaya leaf concentration (0 g / l, 30 g / l, 70 g / l, 100 g / l, 120 g / l), and the second factor is a mixture of kerosene. The treatment was repeated three times. The study was conducted at the Agrotechnology Laboratory, Agriculture Faculty Muhammadiyah University, South Tapanuli. The results showed that papaya leaves could kill S. exigua larvae. The highest mortality of S. exigua worms There were statistically significant non-treatments among treatments.

scholarly journals PENGARUH JENIS BAHAN PENGEMAS TERHADAP DAYA TAHAN CABAI MERAH (Capsicum annum L.) SELAMA PENYIMPANAN DINGIN

SIMBIOSA ◽  
2017 ◽  
Vol 2 (1) ◽  
Author(s):  
Fenny Agustina ◽  
Agam Akhmad Syaukani ◽  
Fatimah Sahara
Keyword(s):  
Experimental Method ◽  
Cold Storage ◽  
Packaging Materials ◽  
Plastic Film ◽  
Test Results ◽  
Capsicum Annum ◽  
Control Film ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Texture Attributes

The study was conducted over two months, from March to April 2012. This study aims to analyze the influence of type of packaging materials for durability of chilli (Capsicum annum L.) during cold storage. The method used in this study is an experimental method using a completely randomized design (CRD), which consists of four types of packaging materials, namely of chilli are packed in plastic PE, PP, PS, PS + control film with a red pepper that is stored without packaging. The parameters analyzed are severe shrinkage and the organoleptic (texture, color, aroma and overall). From the test results obtained ANAVA Fhitung value of 203,869 is greater than the value Ftabel, both at the level of 5% for 3,480 and 5,990 at 1% level. For the organoleptic value of PS + plastic film gives the highest yield in each test the texture attributes of 6,678. 6,500 color, aroma and overall 6,122 and 6,722. PP plastic gives the lowest result in each test the texture attributes of 3,156, 3,556 color, aroma and overall 3,333 and 3,300, this indicates that there is a very significant effect between the type of packaging materials for durability chili during cold storage.

scholarly journals In vitro growth of Cattleya nobilior Rchb. f.: culture media, sealing systems and irradiance

2021 ◽  
Vol 51 ◽  
Author(s):  
Kaliana Gottschalk de Freitas ◽  
José Carlos Sorgato ◽  
Jackeline Schultz Soares ◽  
Luan Marlon Ribeiro
Keyword(s):  
Plant Height ◽  
Natural Ventilation ◽  
Culture Medium ◽  
Culture Media ◽  
Ventilation System ◽  
Light Condition ◽  
Leaf Length ◽  
In Vitro Growth ◽  
Randomized Design

ABSTRACT Some factors may influence the in vitro culture of native orchids, especially the culture medium, sealing system and light condition. This study aimed to evaluate the addition of banana pulp and peel in the formulation of culture media, sealing system and irradiance, in the in vitro growth of Cattleya nobilior Rchb. f. A completely randomized design was used in a factorial scheme with five culture media [Murashige & Skoog supplemented or not with banana pulp or peel (100 or 200g L-1)], two light conditions [3,000 K LED lamps (43 µmol m-2 s-1 or 86 µmol m-2 s-1)] and two sealing systems (natural and conventional ventilation), with six replicates of one vial each. The plant height, diameter of the largest pseudobulb, length of the largest root and of the largest leaf, fresh weight and number of leaves, roots and shoots were evaluated. The use of both the banana pulp and peel as additives in the culture medium formulation promoted the C. nobilior growth. The natural ventilation system increased the plant height, pseudobulb diameter and leaf length, while the conventional system promoted tillering.

scholarly journals EFEKTIVITAS INFUSA DAUN JAMBU BIJI (Psidium guajava) SEBAGAI PRESERVATIF ALAMI PADA KARKAS PUYUH

2021 ◽  
Vol 46 (2) ◽  
pp. 127
Author(s):  
Eulis Tanti Marlina ◽  
Ellin Harlia ◽  
Yuli Astuti Hidayati ◽  
Deden Zamzam Badruzzaman ◽  
Wowon Juanda
Keyword(s):  
Shelf Life ◽  
Experimental Method ◽  
Psidium Guajava ◽  
Antimicrobial Compounds ◽  
Natural Preservative ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Guava Leaves ◽  
Total Bacteria

Guava leaves can be used as a natural preservative in quail carcass because they contain antimicrobial compounds such as flavonoid and tannin. This study studied the effect of using guava leaves infusion on quail carcass on the total bacteria, Enterobacteriaceae, and early spoilage.  The research used an experimental method with a completely randomized design, 3 treatments of guava leaves infusion concentration, namely P1 = 25%, P2 = 50%. And P3 = 75%, each treatment was repeated 6 times.  The application of infusion to quail carcass was carried out by immersion for 15 minutes.  Data were analyzed using ANOVA and Turkey’s test.  The results showed that the total bacteria and Enterobacteriaceae before immersion were 3.80 x 107 cfu/g and 1.90 x 104 cfu/g, respectively.  Soaking quail carcass in guava leaves infusion was able to reduce the total bacteria and Enterobacteriaceae to 72.15% and 78.50%, respectively.  The early spoilage was inversely proportional to the total bacteria and Enterobacteriaceae resulted in longer shelf life.

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