scholarly journals UJI PENGGUNAAN BERBAGAI MEDIA TUMBUH DAN PEMBERIAN PUPUK GROWMORE PADA AKLIMATISASI TANAMAN ANGGREK Dendrobium

2019 ◽  
Vol 15 (2) ◽  
pp. 105-114
Author(s):  
GUSTI MARLINA ◽  
MARLINDA MARLINDA ◽  
HENI ROSNETI
Keyword(s):  
Plant Height ◽  
Growth Media ◽  
Growing Media ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Difference Test ◽  
Honestly Significant Difference Test

The research objective was determine the effect of various growth media and growmore fertilizer on the acclimatization of Dendrobium orchids. study used a Completely Randomized Design (CRD) Factorial consisting of 2 factors, the first is (M) of growing media which consists of 3 levels, namely: M1 (medium fern root), M2 (wood charcoal medium), M3 (coir media coconut) and the second is (G) growmore fertilizer which consists of 4 levels, namely: G0 (without Growmore fertilizer), G1 (Growmore fertilizer 2 g / liter), G2 (fertilizer Growmore 4 g / liter), G3 (Growmore fertilizer 6 g / liter). Determine the growth of orchid plants, observations were made with the following parameters Growth Percentage (%), Plant Height (cm), Number of leaves (strands), and Number of tillers (stems). Data obtained were analyzed statistically, then continued with the Honestly Significant Difference Test (BNJ) at the level of 5%. The results showed that the single treatment of the use of growing media did not have a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). The growth of growmore fertilizer also did not show a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). Interaction of the use of various growth media and growmore fertilizer did not show a significant effect on the percentage of growth (%), plant height (cm), number of leaves (strands), and number of tillers (stems).

scholarly journals PENGGUNAAN 2,4-D UNTUK INDUKSI KALUS KLON KAKAO UNGGUL SULAWESI 1

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Yulianti Rasud ◽  
Moh. Habil ◽  
Tony Tony
Keyword(s):  
Callus Induction ◽  
Callus Formation ◽  
Culture Techniques ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Difference Test ◽  
Honestly Significant Difference Test

ABSTRACT The multiplication of cocoa clones in conventional Sulawesi has not yet been able to fulfill the demand for large quantities of seeds because it is limited by the number of shoots and branches ready to be tapped, connected and oculated and takes longer to produce large quantities of seeds. One alternative in overcoming this problem is plant proragation using tussue culture techniques.  The aim of this experiment was to determine the appropriate of 2,4-D for callus induction of superior cocoa clones Sulawesi via in vitro culture.  This experiment used Completely Randomized Design with five treatments, namely 0.50 ppm 2,4-D, 0.75 ppm 2,4-D, 1.00 ppm 2,4-D, 1.25 ppm 2,4-D and 1.50 ppm 2,4-D.  Parameters observed consisted of the time, percentage, color and texture of calli.  Data was analized by using analysis of variance and differences between mean treatments were determined by Honestly Significant Difference Test at 5% level.  Results of this experiment indicated that the ability of different callus induction at various concentrations of 2,4-D for superior cocoa clones in Sulawesi 1 was tried.  it was obtained the quickest callus formation at concentration 0.50ppm 2,4-D namely average 4.22 WAC with the percentage of callus formation was up to 99,33%. Keywords: Callus Induction, Clones Sulawesi 1, 2,4-D ABSTRAK Perbanyakan klon kakao Sulawesi secara konvensional saat ini belum dapat memenuhi permintaan bibit dalam jumlah besar karena sangat dibatasi oleh jumlah tunas dan cabang yang siap disetek, disambung, dan diokulasi serta dibutuhkan waktu yang lebih lama untuk menghasilkan bibit dalam jumlah besar. Salah satu alternatif dalam mengatasi masalah tersebut adalah perbanyakan tanaman dengan menggunakan teknik kultur jaringan.  Penelitian ini bertujuan untuk memperoleh protokol yang tepat dalam menginduksi kalus sebagai upaya awal dalam perbanyakan tanaman kakao melalui embryogenesis. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 5 level perlakuan yaitu 0,50 ppm 2,4-D, 0,75 ppm 2,4-D, 1,00 ppm 2,4-D, 1,25 ppm 2,4-D dan 1,50 ppm 2,4-D. Pengamatan dilakukan terhadap saat muncul kalus, persentase eksplan berkalus, warna kalus dan tekstur kalus.  Data diolah dengan analisis ragam dan perbedaan antar perlakuan ditentukan dengan Uji Beda Nyata Jujur pada taraf 5%. Hasil penelitian menunjukkan kemampuan induksi kalus berbeda pada berbagai konsentrasi 2,4-D untuk klon kakao unggul Sulawesi 1 yang dicobakan. Saat muncul kalus paling cepat diperoleh pada konsentrasi 0,5 ppm 2,4-D yaitu rata-rata 16,67 HST dengan persentase pembentukan kalus tertinggi mencapai 99,33%.  Selanjutnya, warna dan tekstur kalus yang dihasilkan yaitu remah putih dan remah kecoklatan. Kata Kunci: Induksi Kalus, Klon Sulawesi 1, 2,4-D.

GROWTH RESPONSE OF BEHA GRASS (Brachiaria humidicola) FERTILIZED WITH GOAT MANURE

2021 ◽  
Vol 2 (3) ◽  
Author(s):  
Budiman Budiman ◽  
Natsir Sandiah ◽  
La Malesi
Keyword(s):  
Plant Height ◽  
Root Length ◽  
New Production ◽  
Brachiaria Humidicola ◽  
Randomized Design ◽  
Length Data ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Goat Manure ◽  
Difference Test

This study aimed to determine the best dosage of goat manure as a natural fertilizer on Beha grass(Brachiaria humidicola). This study used 64 poles of Beha grass grown in polybags divided into 16plots, and it used a completely randomized design (CRD) to analyze the data. This study consist of 4treatments that is P0 (0 ton/ha of goat manure), P1 (10 ton/ha of goat manure), P2 (15 ton/ha of goatmanure), and P3 (20 ton/ha of goat manure). This study's variables were plant height, number of leaves,numbers of tillers, new production, and root length. Data analysis used ANOVA variance continued withreal honest difference test. The result of this study showed that averages of plant height (cm) wereP0=26, P1=50.5, P2=51.5, and P3=52.75. Averages of the number of leaves were P0=22, P1=110,P2=160, and P3=185. Averages of numbers of tillers were P0=7, P1=25, P2=37, and P3=38. Averages ofnew production (g) were P0=17.75, P1=123.5, P2=178, and P3=192.25. Averages of root length (cm)were P0=37.8, P1=39.5, P2=41.775, and P3=49.5. Fertilization with natural fertilizer from goat manurehas a genuine effect (P<0.01) on the growth of Beha grass. The best dosage of fertilization with goatmanure was 15 ton/ha.

scholarly journals DETECTION AND TRANSMISSION OF RICE STUNT VIRUS ON CIHERANG AND SITU BAGENDIT VARIETIES

2019 ◽  
Vol 18 (2) ◽  
pp. 169
Author(s):  
Selvi Helina ◽  
Sri Sulandari ◽  
Sedyo Hartono ◽  
Andi Trisyono
Keyword(s):  
Plant Height ◽  
Incubation Period ◽  
Rice Production ◽  
Rice Plants ◽  
Inhibition Of Growth ◽  
Randomized Design ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Rice Ragged Stunt Virus

Detection and Transmission of  rice stunt virus on Ciherang and Situ Bagendit Varieties. The explosion of brown planthoppers recently has caused reduction of rice production in Indonesia. Brown planthoppers do not only act as pest, but also transmit Rice grassy stunt virus (RGSV) and Rice ragged stunt virus (RRSV). Detection of the existence of the two viruses in rice plants and vector insects is important to be done to ensure that the virus is infected with the vector. The aim of this research is to detect the existence of virus in varieties of Ciherang and Situ Bagendit as a result of transmission in the laboratory and to find out the ability of brown planthoppers to transmit stunt virus to both of the varieties. This research was compiled using Completely Randomized Design (CRD) with 4 treatments, namely healthy rice plants of Ciherang and Situ Bagendit varieties, Ciherang and Situ Bagendit varieties which were infested by brown planthoppers each with 5 repetitions. The parameters observed were incubation period, symptoms, plant height, number of leaves and incidence of disease. The data on plant height, number of leaves and incidence of disease were analyzed using ANOVA and continued with the Least Significant Difference (LSD) test at the level of 5%. The results showed that Ciherang and Situ Bagendit varieties were only positively infected by Rice ragged stunt virus. The results of the rice transmission showed that Ciherang variety had a faster incubation period of 10 DAI while Situ Bagendit was 14 DAI, but the two varieties showed an inhibition of growth in plant height and number of leaves compared to healthy plants with each incidence of 51.3% and 46.3%.

scholarly journals THE EFFECT OF HYDROPONIC NUTRITION LEVELS ON THE GROWTH OF MUSTARD (BRASSICA SP.) PLANTS

2020 ◽  
Vol 1 (1) ◽  
pp. 40-52
Author(s):  
Arie Hapsani Hasan Basri ◽  
Mahmudah Mahmudah
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Research Method ◽  
Growth And Yield ◽  
Leaf Width ◽  
Randomized Design ◽  
Significant Difference ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Difference Test

The main roles of plant growth was nutrition. This research aims to determine the concentration of nutrients that can provide the best growth and yield on various mustard. The research method  used is a factorial Complete Randomized Design (RBD) with three replications and then analyzed using the Least Significant Difference test (LSD) at a level of 5%. The treatment of vegetable types is as follows: S1 = mustard pakchoy, S2 = mustard samhong, S3 = mustard pagoda, S4 = mustard pakchoy mini. Treatment with nutrition is the AB mix fertilizerzs number of leaves solution as follows: N1 = 1000 ppm, N2 = 1200 ppm, N3 = 1400 ppm, N4 = 1600 ppm, N5 = 1800 ppm. The results showed that plant height, number of leaves, leaf width and wet weight of the pagoda and mini pakchoy plants were not significantly different at 1000 ppm, 1200 ppm, 1400 ppm, 1600 ppm and 1800 ppm. Number of leaves of pakchoy significantly different at 1400 ppm. While the number of leaves and leaf width of samhong plants were not significantly different at each treatment, but were significantly different from plant height at 1600 ppm and significantly different from the wet weight at 1400 ppm

scholarly journals The Effect of Cocopeat and Charcoal Combination in Growing Media of Green Mustard (Brassica rapa L.) Var. Parachinensis Growth

2020 ◽  
Vol 7 (1) ◽  
pp. 62-66
Author(s):  
Masniar Vina Kesti ◽  
Bambang Irawan ◽  
Priyambodo Priyambodo ◽  
Martha Lulus Lande
Keyword(s):  
Dry Weight ◽  
Growing Media ◽  
Randomized Design ◽  
Vigor Index ◽  
Growing Medium ◽  
Significant Difference ◽  
Seedling Roots ◽  
Completely Randomized Design ◽  
Water Gas ◽  
Difference Test

Green mustard is one type of vegetable that is easily cultivated and has good prospects to increase farmers' income and community nutrition. Growing media is a material that is useful as a place to stand upright seedlings, to develop seedling roots, to store water, gas, and nutrients needed for seedling growth. The purpose of this study is a). to determine the effect of using a combination of cocopeat and charcoal as a medium for planting mustard greens, b). to get the best dosage of the composition of the growing medium on the growth of mustard greens. The study was conducted in a completely randomized design (CRD) with 4 treatments and 6 replications. The data were analyzed by analysis of variance (ANOVA) and further tested with the Least Significant Difference test (LSD) = 5%. The results showed that the use of cocopeat growing media and charcoal influenced germination and vigor index. Cocopeat and charcoal have a significant effect on fresh and dry weight and chlorophyll content of mustard greens. The mixture of cocopeat growing media and charcoal on P1 did not affect the growth of green mustard on the dry weight. Fresh weight gave a significant effect on K1 and P1 was also significantly different from P2 and P3.

scholarly journals Pengaruh Pencampuran Media Tanam Serta Beda Ukuran Benih Terhadap Pertumbuhan Biji Kamboja Jepang (Adenium crimsonstar)

2017 ◽  
Vol 1 (1) ◽  
pp. 7
Author(s):  
Kukuh Dwi Styawan ◽  
Rustam Baraq Noor ◽  
Abdul Rofik
Keyword(s):  
Plant Height ◽  
Seed Size ◽  
Seed Length ◽  
Growing Media ◽  
Randomized Design ◽  
Significant Difference ◽  
Seed Growth ◽  
Media Interaction ◽  
Completely Randomized Design ◽  
Different Grain Size

As well as the growing influence of media mixing different grain size of the seed to growth frangipani Japan (Adenium crimsonstar). This study aims to determine the effect of mixing different media as well as the planting of seed size on seed growth frangipani Japan (Adenium crimsonstar). The study was conducted in April through July 2010, in the district of Ulu Loa Janan, Kutai regency. This study uses a completely randomized design (CRD) with 4 x 3 factorial analysis consisted of four replications. The first factor is the growing media 4 levels: m0: sand medium (control), m1: sand + fine brick (3: 1), m2: sand, ground roasted (3: 1), m3: finely + sand + bricks ground fuel (2 : 1: 1). The second factor is the size of the seed 3 levels: b1: weight 0,02gram / 0.9 to 1.0 cm long seed / seed, b2: 0,01gram / 0.5-0.7 cm long beans / seeds, b3: weight of 0,008 gram / seed length from 0.3 to 0.4 cm / seed. The results showed that treatment Growing Media significant effect on all parameters of the study. Treatment m2 (sand + soil fuel) showed the highest plant height is 5.44 cm, while the treatments m1 (fine brick sand +) showed the smallest plant height is 3.07 cm, the results showed that the different treatment of seed size did not significantly affect all parameter studies. Media interaction and different planting seed size showed no significant difference in all parameters of the study.

scholarly journals تأثير تراكيز مختلفة من السكر في بيئة زراعة الأنسجه على نمو نبات الورد صنف ساره (Sara)

2020 ◽  
Vol 16 (1) ◽  
pp. 49-62
Author(s):  
محي الدين جمعة عبد الله
Keyword(s):  
Growth Parameters ◽  
Growth Media ◽  
Fresh Weight ◽  
Negative Results ◽  
Randomized Design ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Number Of Branches

The effect of adding different sugar concentrations of five treatments (T1, T2, T3, T4, T5) on rose growth CV Sarah in vitro was carried out in the Sudan University Laboratories. Data were collected for plant height, number of branches, number of leaves, and fresh weight. Statistical analysis was conducted using a completely randomized design, to find the least significant difference to compare between treatment means for MS (Murashige and Skoog) medium. Data analysis showed that the application of sugar at concentrations of 15, 30, and 45gms gave the highest means for the different growth parameters and fresh weight at a significant level, whereas the addition of sugar at 60gms concentration and sugar-free gave no significant increases. This showed clearly that addition of sugar was essential for plant growth, greater concentrations gaviz 60gm negative results, Addition of sugar at the above-mentioned concentration from 15mg,45gm to parameters studied the growth media in vitro gave a positive increase in rose growth.

scholarly journals INITIATION OF GRAPE (VITIS VINIFERA L.) ON DIFFERENT STERILIZATION TECHNIQUES

2019 ◽  
Vol 5 (2) ◽  
pp. 91
Author(s):  
Ratu Mentari Dewi ◽  
Ramal Yusuf ◽  
Sri Anjar Lasmini ◽  
Hawalina Hawalina
Keyword(s):  
Callus Formation ◽  
Plant Biotechnology ◽  
Experimental Unit ◽  
Vitis Vinifera L ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Difference Test ◽  
Honestly Significant Difference Test

The most crucial step during the development of tissue culture is the method of explant sterilization. Especially, explants is sourced directly from fields that were more susceptible to microbial contaminations. This study aimed to obtain sterilization technique  from several sterilant for reduced contamination and support growth of shoots grape in vitro. This research was conducted at the Laboratory of Plant Biotechnology, Faculty of Agriculture, University of Tadulako, during January to April 2018. The study used a factorial completely randomized design, one factor was tested i.e S1 = Taft 8,3 g/L (0,1% carbendazim), S2 =  taft 25 g/L (0,3% carbendazim),  S3 = taft 41,67 g/L (0,5% carbendazim), S4 = Taft 8,3 g/L (0,1% carbendazim) + HgCl2 0,1%, S5  = Taft 25 gr/L (0,3% carbendazim) + HgCl2 0,1% dan S6 = 41,67 gr/L  (0,5% carbendazim) + HgCl2 0,1 %. There were six treatment and each treatment was replicated three times to obtain 18 experimental units. Each experimental unit using two explant. Data were analyzed using analysis of variance and followed by Honestly Significant Difference test at level of 5% if the treatment effects were significant. The results showed sterilization technique using taft 41,67 gram / L containing 0.5% carbendazim for 30 minutes, followed by 70% alcohol for 30 seconds and HgCl2 0,1% for 10 minutes gave free contamination for explant.whereas, Taft 8.3 g / L (Carb 0.1%) for 30 min, followed by 70% alcohol for 30 seconds is a sterilization technique that does not inhibit explant growth as indicated from the most callus formation.

scholarly journals Pertumbuhan Planlet Nenas (Ananas comosusL. Merr.) Varietas Smooth Cayenne Hasil Kultur In Vitro pada Beberapa Konsentrasi BAP dan Umur Plantlet

2013 ◽  
Vol 1 (1) ◽  
pp. 54
Author(s):  
Ramadhani Dwi Santoso ◽  
. Sobir
Keyword(s):  
Block Design ◽  
Error Level ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Randomized Complete Block Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Difference Test ◽  
Honestly Significant Difference Test

<p style="text-align: justify;">The aim of this research is to study the effects of synthetic cytokinin (6-benzylaminopurine/BAP) treatments on two groups of pineapple (Ananas comosusL.Merr.) plantlets differentiated by plantlet ages. The research used the Factorial Experiment on Randomized Complete Block Design with two factors, which are BAP concentrations (0 ppm, 25 ppm, 50 ppm, and 75 ppm) and plantlet division by age grouping (52 days and 69 days), with three replications. It was then followed by Tukey’s Honestly Significant Difference Test at error level of 5%. The results show that BAP treatments with the concentration used in this research significantly inhibits the growth of pineapple plantlets observed on four variables (number of leaves, leave length, plantlet height, and plantlet diameter), while age grouping treatments didn’t show any significant effects, except on the plantlet height at 8 and 14 weeks after treatment. There are also treatment-related interactions which significantly affects the number of leaves and plantlet height. It was then suggested that lower concentrations of cytokinin are required if similar research is to be conducted in the future.</p><p>Keywords : pineapple, post-acclimatization, propagation, 6-benzylaminopurine</p>

scholarly journals KECERNAAN IN VITRO SILASE SAMPAH SAYUR DAN DAUN GAMAL MENGGUNAKAN MIKROORGANISME RUMEN KAMBING

2015 ◽  
Vol 2 (3) ◽  
pp. 17
Author(s):  
Indrayani Indrayani ◽  
Harapin Hafid ◽  
Dian Agustina
Keyword(s):  
Organic Matter ◽  
Analysis Of Variance ◽  
Dry Matter ◽  
Vegetable Waste ◽  
Dry Matter Digestibility ◽  
Randomized Design ◽  
Honestly Significant Difference ◽  
Significant Difference ◽  
Completely Randomized Design

ABSTRACTThis study aims to determine the digestibility of dry matter and organic matter level waste silage mixed vegetables and Gliricidia leaves were tested in vitro. This study used a completely randomized design with 3 treatments and 3 replications. Each of these treatments is R0 (Gliricidia leaves 100%), R1 (Gliricidia leaves 70% + 30% silage vegetable waste), and R2 (Gliricidia leaves 40% + 60% silage vegetable waste). Data were analyzed using analysis of variance (ANOVA) and further testing using the test Honestly Significant Difference (HSD). The results of ANOVA showed that the mixture of vegetable waste silage was highly significant (p <0.05) on dry matter digestibility and significantly (p <0.05) on the digestibility of organic matter. It can be concluded that the mixed of vegetable waste silage and Gliricidia leaves can improved digestibility of dry matter and organic matter, treatment of 40% and 60% Gliricidia leaves plus waste vegetable produce silage dry matter digestibility and percentage of organic matter is best (72,24% and 68,19%).Keyword: Silage vegetable waste, gliricidia leaves, dry and organic matter digestibilityABSTRAKPenelitian ini bertujuan untuk mengetahui kecenaan bahan kering dan bahan organik tingkat campuran silase sampah sayur dan daun gamal yang diuji secara in vitro. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 3 perlakuan dan 3 ulangan. Masing-masing perlakuan tersebut ialah R0 (daun gamal 100%), R1 (daun gamal 70% + silase sampah sayur 30%), dan R2 (daun gamal 40% + silase sampah sayur 60%). Data yang diperoleh dianalisis dengan menggunakan analisis sidik ragam (ANOVA) dan uji lanjut menggunakan uji Beda Nyata Jujur (BNJ). Hasil analisis sidik ragam menunjukkan bahwa campuran silase sampah sayur berpengaruh sangat nyata (p<0,05) terhadap kecernaan bahan kering dan berpengaruh nyata (p<0,05) terhadap kecernaan bahan organik. Dapat disimpulkan bahwa perlakuan campuran silase sampah sayur dan daun gamal dapat meningkatkan kecernaan bahan kering dan bahan organik, perlakuan 40% daun gamal dan 60% silase sampah sayur menghasilkan persentase kecernaan bahan kering dan bahan organik yang terbaik yaitu (72,24% dan 68,19%).Kata kunci : Silase sampah sayur, daun gamal, kecernaan bahan kering, dan bahan organik.

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