scholarly journals Anticoagulant Activity of Mangrove (Avicennia alba) Leaves Extract in Vitro

2021 ◽  
Vol 26 (2) ◽  
pp. 110-116
Author(s):  
Riza Rusyiana ◽  
Ima Arum Lestarini ◽  
Candra Dwipayana Hamdin ◽  
Handa Muliasari
Keyword(s):  
Human Blood ◽  
Anticoagulant Activity ◽  
Ethanol Extract ◽  
Positive Control ◽  
Sulphated Polysaccharide ◽  
Human Blood Cells ◽  
Coagulant Activity ◽  
Human Blood Samples ◽  
Avicennia Alba

Anti-coagulants are substances used to prevent the formation of thromboembolism, one of the causes of cardiovascular disease. Since cardiovascular is a disease as the leading cause of death in the world, exploration of novel and more effective anticoagulant is very needed. Mangroves contain flavonoid and sulphated polysaccharide compounds that potential as anti-coagulants. This study aimed to determine the anti-coagulant activity of ethanol extract and fractions of mangrove leaves (Avicennia alba) in human blood cells in vitro. A. alba leaves were collected at Teluk Ekas, East Lombok, Indonesia. The leaves were macerated with 96% ethanol (3x24h) and then evaporated. The extract was then fractionated to obtain the n-hexane, chloroform, and residual fractions. Anti-coagulant activity of ethanol extract and the three fractions were tested in vitro to five human blood samples by the method of Lee-White and Eustrek, compared to heparin as a positive control. Phytochemical screening of the ethanol extract indicated that A. alba leaves extract contains alkaloids, tannin, saponin, flavonoids, sulphated polysaccharide, and terpenoid. The results of Lee-White method were the ethanol extract (1 mg.mL-1), and three fractions (0.5 mg.mL-1) have anti-coagulant activity. Statistical analysis showed that the anti-coagulant activity of the ethanol extract was significantly different (P<0.05) compared to the normal group and fractions and were not significantly different (P>0.05) compared to heparin. Ethanol extract and the three fractions of A. alba leaves have anti-coagulant activity in vitro. The anti-coagulant activity of ethanol extract (1 mg.mL-1) was comparable to heparin (25 IU) and was better than the three extract fractions (0.5 mg.mL-1). Further research is needed to test the anticoagulant activities of isolated sulphated polysaccharides and flavonoid that can be developed as anticoagulant agents. 

In Vitro Distribution of Diacetyl Didemnin B in Human Blood Cells and Plasma

1989 ◽  
Vol 7 (2) ◽  
pp. 123-128 ◽  
Author(s):  
Jerry L. Phllips ◽  
Rowena Schwartz ◽  
Daniel D. Von Hoff
Keyword(s):  
Human Blood ◽  
Blood Cells ◽  
Didemnin B ◽  
Human Blood Cells

scholarly journals Daya Antibakteri Ekstrak Etanol Ketepeng Cina (Senna alata) terhadap Staphylococcus aureus dan Escherichia coli secara In Vitro

2012 ◽  
Vol 13 (1) ◽  
pp. 63
Author(s):  
Taswin Yacob ◽  
Rita Endriani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diffusion Method ◽  
Randomized Design ◽  
Senna Alata ◽  
Treatment Of Infection ◽  
Range Test

The benefit and efficacy of ketepeng cina (Senna alata) in the treatment of infection has shown that have antibacterialactivity, inhibiting and killing bacteria that cause infection. The objective of this study was evaluate the antibacterialactivity of ketepeng cina against Staphylococcus aureus dan Escherichia coli in vitro. This study was a laboratoryexperimental research which use completely randomized design with diffusion method. Ethanol extract of Sennaalata leaves devided into 4 doses, i.c. 100, 50, 25 and 12.5. Amoxiclave were used as positive control and aquadestnegative control. The data were analyzed by Analysis of Varian continued with Duncan’s Multiple Range Test. Theresult of this study showed that antimicrobial activity of ethanol extract Senna alata leaves inhibited the growth ofStaphylococcus aureus, but not Escherichia coli. The optimum effect was showed given by the concentration 100at 17.7 mm.

scholarly journals EFEK EKSTRAK ETANOL BIJI LABU KUNING (CUCURBITA MOSCHATA DUCHESNE) SEBAGAI ANTELMINTIK PADA CACING GELANG (ASCARIDIA GALLI)

2020 ◽  
Vol 7 (1) ◽  
pp. 11-18
Author(s):  
Noni Zakiah ◽  
Vonna Aulianshah ◽  
T. Maulana Hidayatullah ◽  
Faridah Hanum
Keyword(s):  
Ethanol Extract ◽  
Positive Control ◽  
The Body ◽  
Whole Body ◽  
In Vitro Test ◽  
Cucurbita Moschata ◽  
Ascaridia Galli ◽  
Group I ◽  
Pumpkin Seeds

Kegunaan labu kuning di Indonesia masih sebatas daging buah yang dapat diolah menjadi panganan seperti kue basah, kolak dan sayur berkuah. Secara empiris, biji labu kuning telah digunakan untuk mengatasi cacingan. Penelitian ini dilakukan untuk mengetahui mortalitas cacing gelang (Ascaridia galli) dalam ekstrak etanol biji labu kuning (Cucurbita moschata Duchesne). Penelitian ini menggunakan 25 ekor Ascaridia galli yang dibagi menjadi 5 kelompok, kelompok I kontrol negatif menggunakan larutan NaCl fisiologis, kelompok II kontrol positif menggunakan larutan pirantel pamoat 0,5 %, kelompok III, IV dan V berturut-turut menggunakan 25 mg/ml, 50 mg/ml dan 100 mg/ml ekstrak etanol biji labu kuning. Parameter penelitian ini ditentukan dengan melihat persentase nilai skor pasca inkubasi 12 jam, 24 jam, dan 36 jam. Skor 3 diberikan apabila seluruh tubuh Ascaridia galli bergerak, skor 2 diberikan jika hanya sebagian tubuh Ascaridia galli bergerak, skor 1 jika Ascaridia galli diam tetapi masih hidup, dan skor 0 apabila Ascaridia galli mati. Hasil uji in vitro dengan perlakuan 25 mg/ml ekstrak etanol biji labu kuning menyebabkan kematian 3 ekor Ascaridia galli  atau 60% pasca inkubasi 36 jam, sedangkan ekstrak etanol biji labu kuning dengan perlakuan 50 mg/ml, 100 mg/ml dan kelompok kontrol positif mengakibatkan kematian 4 ekor Ascaridia galli atau 80% pasca inkubasi 36 jam. Dari hasil penelitian disimpulkan bahwa ekstrak etanol biji labu kuning (Cucurbita moschata Duchesne) dosis 25 mg/ml, 50 mg/ml, dan 100 mg/ml secara in vitro dalam waktu 36 jam mampu mengakibatkan mortalitas Ascaridia galli. The use of yellow pumpkin in Indonesia is still limited to fruit meat that can be processed into snacks such as soggy cakes, porridge and vegetable soup. This research was conducted to determine the mortality of Ascaridia galli in ethanol extract of yellow pumpkin seeds (Cucurbita moschata Duchesne). This study used 25 Ascaridia galli which were divided into 5 groups, group I was negative control using physiological NaCl solution, group II was positive control using 0.5% pirantel pamoate solution, group III, IV and V respectively used 25 mg / ml, 50 mg/ml and 100 mg/ml ethanol extract of yellow pumpkin seeds. The parameters of this study were determined by looking at the percentage of post-incubation scores 12 hours, 24 hours, and 36 hours. A score of 3 is given if the whole body of Ascaridia galli moves, a score of 2 is given if only part of the body of Ascaridia galli moves, a score of 1 if Ascaridia galli is still but still alive, and a score of 0 if Ascaridia galli dies. In vitro test results with 25 mg/ml ethanol extract of pumpkin seeds caused 3 deaths of Ascaridia galli or 60% after incubation for 36 hours, while ethanol extract of yellow pumpkin seeds treated with 50 mg / ml, 100 mg/ml and positive control group resulting in the death of 4 Ascaridia galli or 80% after 36 hours incubation. From the results of the study concluded that the ethanol extract of yellow pumpkin seeds (Cucurbita moschata Duchesne) doses of 25 mg / ml, 50 mg / ml, and 100 mg / ml in vitro within 36 hours can lead to Ascaridia galli mortality.

scholarly journals No Evidence for XMRV in German CFS and MS Patients with Fatigue Despite the Ability of the Virus to Infect Human Blood Cells In Vitro

PLoS ONE ◽  
2010 ◽  
Vol 5 (12) ◽  
pp. e15632 ◽  
Author(s):  
Oliver Hohn ◽  
Kristin Strohschein ◽  
Alexander U. Brandt ◽  
Sandra Seeher ◽  
Sandra Klein ◽  
...  
Keyword(s):  
Human Blood ◽  
Blood Cells ◽  
Human Blood Cells

Identifikasi Antiinflamasi Partisi Metanol, n-Heksan dan Ekstrak Etanol Daun Kemangi (Ocimum Americanum L.) Secara In Vitro

2021 ◽  
Vol 1 ◽  
pp. 820-828
Author(s):  
Annas Pamening ◽  
W Wirasti ◽  
S Slamet ◽  
Urmatul Waznah
Keyword(s):  
Diclofenac Sodium ◽  
Ethanol Extract ◽  
Positive Control ◽  
Hypotonic Solution ◽  
Stabilization Method ◽  
Membrane Stabilization ◽  
Anti Inflammatory ◽  
Red Blood Cell Membranes ◽  
Ocimum Americanum

AbstractBasil plant (Ocimum americanum) is efficacious as an anti-inflammatory and analgesic activity. According to research by Sarma and Babu, 2011, Verma and Kothiyal, 2012 showed basil activity as an antioxidant, antimicrobial, anti-diabetic, anthelmintic, antifungal, insecticide, anti-inflammatory, analgesic, and lowering total cholesterol and LDL-C levels. The purpose of this study was to determine the stabilization activity of red blood cell membranes on methanol partitioning, n-hexane partitioning and ethanol extract of basil leaves in vitro. This study used the erythrocyte membrane stabilization method from the induction of a hypotonic solution with samples of methanol partitioning, n-hexane partitioning and ethanol extract to be compared with a positive control, namely Na diclofenac. By analyzing the data using UV-Vis spectrophotometry test. These results were supported by the ANOVA statistical test which stated that there was a difference in each treatment and continued with the Tukey test which stated that there was no difference between 100 ppm diclofenac sodium and 400 ppm ethanol extract.Keywords: Extract, Basil (Ocimum americanum) Leaf, In Vitro. AbstrakTumbuhan Kemangi (Ocimum americanum) berkhasiat sebagai aktivitas sebagai anti-inflamasi dan analgesik. Menurut penelitian Sarma dan Babu, 2011.,Verma dan Kothiyal, 2012 menunjukkan aktivitas kemangi sebagai antioksidan, antimikroba, anti diabetes, antihelmintik, antifungi, insektisida, antiinflamasi, analgesic, dan menurunkan kadar total kolesterol dan LDL-C. Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas stabilisasi membran sel darah merah pada partisi metanol, partisi n-heksan dan ekstrak etanol daun kemangi secara in vitro. Penelitian ini menggunakan metode stabilisasi membran eritrosit dari induksi larutan hipotonik dengan sampel partisi metanol, partisi n-heksan dan ekstrak etanol yang akan dibandingkan dengan kontrol positif yaitu Na diklofenak. Dengan analisis data menggunakan uji spektrofotometri UV-Vis. Hasil ini didukung dengan uji statistik ANOVA yang menyatakan terdapat perbedaan pada setiap perlakuan dan dilanjutkan uji tukey yang menyatakan tidak ada perbedaan pada natrium diklofenak 100 ppm dengan ekstrak etanol konsentrasi 400 ppm.Kata Kunci : Ekstrak, Daun Kemangi (Ocimum americanum), In Vitro.

scholarly journals Potential of Malacca leaf (Phyllanthus emblica) against Salmonella sp

2020 ◽  
Vol 151 ◽  
pp. 01029
Author(s):  
Nuzul Asmilia ◽  
Mahdi Abrar ◽  
Yudha Fahrimal ◽  
Amalia Sutriana ◽  
Yobeswi Husna
Keyword(s):  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Positive Control ◽  
Hexane Extract ◽  
Clear Zone ◽  
Inhibitory Property

Malacca is one of traditional medicine that possesses a potent antimicrobial activity. This study aims to determine the inhibitory activity of Malacca leaf extract on the growth of Salmonella sp in vitro. The bacteria was obtained from Microbiology Laboratory of the Faculty of Veterinary Medicine, Universitas Syiah Kuala. The study was conducted using n-hexane extract, ethyl acetate extract and ethanol of malacca leaves with dilution concentrations of 5%, 25%, and 50%.The inhibitory property of malacca leaf was tested using Kirby-Bauer method. Data were analyzed descriptively. The results of this study indicate that n-hexane extract, ethyl acetate extract and ethanol extract of malacca leaves can inhibit the growth of Salmonella sp. The n-hexane extract of malacca leaves showed a greater inhibition than the ethyl acetate and ethanol extract of malacca leaves. n-hexane extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 1.35 mm (weak), 4.97 mm (moderate), and 12.87 mm (strong), respectively ethyl acetate extract with a concentration of 5%, 25%, and 50% showed average diameter inhibition of 2.00 mm (weak), 5.72 mm (moderate), and 7.58 mm (moderate), whereas in ethanol extract were 0.47 mm (weak), 2.58 mm (weak), and 4.35 mm (weak), repectively. The clear zone areas in negative and positive control were 0.00 mm 20.00 mm, respectively. Malacca leaf extract possess inhibitory property against the growth of the Salmonella sp.

Impact Deposition of Radon Progeny (218Po ,214Po and 210Po) on the Surface of Human Blood: In Vitro Study

2011 ◽  
Vol 110-116 ◽  
pp. 2004-2009 ◽  
Author(s):  
Asaad Hamid Ismail ◽  
Mohamad S. Jaafar
Keyword(s):  
Human Blood ◽  
Radon Concentration ◽  
Absorbed Dose ◽  
Blood Component ◽  
Good Efficiency ◽  
Present Design ◽  
Human Blood Samples ◽  
The Impact ◽  
Exposure Technique

This work presented new irradiation technique to estimate the impact of radon's progeny deposition on the human blood surface on some blood diseases, using CR-39Nuclear Track Detectors (NTDs). The results show that the present design has good efficiency and the loss rate of radon concentration was a little. Amount of the loss of radon concentration during the process of mixing blood component did not affect on the efficiency of exposure technique. Therefore, method of mixing blood component process was successful. As well as, human blood exposure to radon gas (2210±5.1Bq/m3) make thrombocytopenia, and no effect on red blood cell. And rate of radon absorbed dose into the human blood samples is high at 20 minutes.

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