The acute supplementation of combination juice of yellow watermelon (citrullus lanatus thunb.) - plantain (musa paradisiacal var. Sapientum l.) suppress post-exercise blood lactic acid production in rats

2021 ◽  
Vol 9 (2) ◽  
pp. 73-79
Author(s):  
Farida Farida ◽  
Hesti Permata Sari ◽  
Afina Rachma Sulistyaning
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Citrullus Lanatus ◽  
Lactic Acid Production ◽  
Test Dose ◽  
Negative Control ◽  
Muscle Performance ◽  
Sprague Dawley ◽  
Significant Difference ◽  
Swimming Test

Background: Yellow watermelon contains citrulline, which can suppress lactic acid production, while plantains contain potassium which is important for muscle performance. The yellow watermelon and plantain combination juice potential to be a natural sports drink that delays muscle fatigue by suppressing lactic acid production after exercise.Objectives: To determine the effect of yellow watermelon-plantain juice on lactic acid in rats after swimming test.Materials and Methods: This true experimental study used a post-test-only with controlled group design. Thirty Sprague Dawley rats, eight-week-old, male, were divided into five groups, namely positive control (C+), negative control (C-), dose 1 (P1), dose 2 (P2), and dose 3 (P3). The C (+) group received no juice and was not tested swimming, the C (-) group received no juice but was tested swimming, P1 received combined juice up to 1.8 g and tested swimming, P2 received combined juice up to 3, 6 g and tested swimming, P3 received combined juice up to 1.8 g with the addition of 0.27 g granulated sugar and tested swimming. The juice is given 30 minutes before the test. The swim test was performed for three minutes; after that, the blood was taken to test the lactic acid levels. The data were analyzed using the one-way ANOVA and the advanced post-hoc with the least significant difference test.Results: The lactic acid levels in C (+), in C (-), P1, P2, and P3 groups after swimming test were 1.38 mMol / L; 7.14 mMol / L; 3.74 mMol / L; 1.66 mMol; and 2.91 mMol/L. There were differences in levels of lactic acid (p <0.05) in each group after the combination juice intervention was given.Conclusion: Combination juice of yellow watermelon-plantain has an effect on lactic acid levels after swimming test. Dose 2 (3.6 g) was the best because it produces the lowest lactic acid after the swimming test.

scholarly journals Protective effect of titanium tetrafluoride and silver diamine fluoride on radiation-induced dentin caries in vitro

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Beatriz Martines de Souza ◽  
Mayara Souza Silva ◽  
Aline Silva Braga ◽  
Patrícia Sanches Kerges Bueno ◽  
Paulo Sergio da Silva Santos ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Protective Effect ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Negative Control ◽  
Titanium Tetrafluoride ◽  
Silver Diamine Fluoride ◽  
Dentin Caries ◽  
Radiation Induced

AbstractThis in vitro study evaluated the protective effect of titanium tetrafluoride (TiF4) varnish and silver diamine fluoride (SDF) solution on the radiation-induced dentin caries. Bovine root dentin samples were irradiated (70 Gy) and treated as follows: (6 h): 4% TiF4 varnish; 5.42% NaF varnish; 30% SDF solution; placebo varnish; or untreated (negative control). Microcosm biofilm was produced from human dental biofilm (from patients with head-neck cancer) mixed with McBain saliva for the first 8 h. After 16 h and from day 2 to day 5, McBain saliva (0.2% sucrose) was replaced daily (37 °C, 5% CO2) (biological triplicate). Demineralization was quantified by transverse microradiography (TMR), while biofilm was analyzed by using viability, colony-forming units (CFU) counting and lactic acid production assays. The data were statistically analyzed by ANOVA (p < 0.05). TiF4 and SDF were able to reduce mineral loss compared to placebo and the negative control. TiF4 and SDF significantly reduced the biofilm viability compared to negative control. TiF4 significantly reduced the CFU count of total microorganism, while only SDF affected total streptococci and mutans streptococci counts. The varnishes induced a reduction in lactic acid production compared to the negative control. TiF4 and SDF may be good alternatives to control the development of radiation-induced dentin caries.

Protective Effect of 4% Titanium Tetrafluoride Varnish on Dentin Demineralization Using a Microcosm Biofilm Model

2019 ◽  
Vol 53 (5) ◽  
pp. 576-583 ◽  
Author(s):  
Daiana Moreli Soares dos Santos ◽  
Juliana Gonçalves Pires ◽  
Aline Braga Silva ◽  
Priscila Maria Aranda Salomão ◽  
Marília Afonso Rabelo Buzalaf ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Protective Effect ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Extracellular Polysaccharide ◽  
Human Saliva ◽  
Positive Control ◽  
Negative Control ◽  
Titanium Tetrafluoride ◽  
Biofilm Model

This study evaluated the effect of titanium tetrafluoride (TiF4) varnish on the development of dentin carious lesions. Bovine root dentin samples were treated for 6 h with: (A) 4% TiF4 varnish (2.45% F); (B) 5.42% sodium fluoride (NaF) varnish (2.45% F); (C) 2% chlorhexidine (CHX) gel – positive control; (D) placebo varnish; or (E) untreated – negative control (n = 4 × biological triplicate, n = 12). Treated dentin samples were exposed to human saliva mixed with McBain saliva (1:50) for the first 8 h in 24-well plates. Thereafter, the medium was removed, and McBain saliva containing 0.2% sucrose was applied for 16 h. From days 2 to 5, McBain saliva with sucrose was replaced daily (37°C, 5% CO2). The demineralization was measured using transverse microradiography, while the effect on biofilm was analyzed using viability, extracellular polysaccharide (EPS), and lactic acid production assays. The data were statistically analyzed (p < 0.05). All treatments (fluorides and CHX) significantly reduced the biofilm viability compared to placebo varnish and negative control. However, none of them was able to reduce the colony-forming unit counting for total microorganism, total streptococci, and Streptococcus mutans. NaF significantly reduced the number of Lactobacillus sp. compared to negative control. No effect was seen on lactic acid production neither on EPS synthesis, except that CHX significantly reduced the amount of insoluble EPS. Both fluorides were able to reduce dentin demineralization compared to placebo varnish and negative control; TiF4 had a better effect in reducing mineral loss and lesion depth than NaF. Therefore, TiF4 varnish has the best protective effect on dentin carious lesion formation using this model.

Screening of Lactic Acid Fermentation by Rhizopus oryzae on Banana Peel Using Two-Level Factorial Analysis

2022 ◽  
Vol 16 (1) ◽  
pp. 12
Author(s):  
Nor Atikah Husna Ahmad Nasir ◽  
Nurul Syafiqah Mohd Yaminudin ◽  
Atikah Kamaludin ◽  
Sharir Aizat Kamaruddin ◽  
Siti Nurbalqis Aziman
Keyword(s):  
Lactic Acid ◽  
Moisture Content ◽  
Acid Production ◽  
Rhizopus Oryzae ◽  
Lactic Acid Production ◽  
Inoculum Size ◽  
Factorial Analysis ◽  
Banana Peel ◽  
Acid Fermentation ◽  
Significant Difference

For ages, pure sugars or edible crops have produced lactic acid. However, a major concern on lactic acid production lies in the cost of the raw materials used. Thus, an alternative to overcome this situation is urgently needed. Characterization of banana peels shows that it contains promising sugar that can be utilized for lactic acid production, which are xylose (0.774 g/L), glucose (0.756 g/L) and fructose (0.532 g/L). Thus, this study aims to screen the potential of banana peel as a substrate by using Rhizopus oryzae through batch fermentation for lactic acid production, as R. oryzae can synthesize lactic acid in low nutrient requirements. Two-level factorial analysis was designed to screen the effects of moisture content (60% and 80%), temperature (27 °C and 40 °C), pH (4.5 and 6.5) and inoculum size (1x104 spores/mL and 1x108 spores/g) on the lactic acid production. Based on the Two-level factorial (2LF) analysis, the highest lactic acid production of 0.0813 g/L was detected at 80 % moisture content, pH 4.5, the temperature of 27 °C and inoculum size of 1x104 spores/g. The findings show that most of the conditions have a significant difference between each other (p<0.05). Therefore, the fermentation of banana peels by R. oryzae could be a promising method to produce a lactic acid concentration.

scholarly journals Improving Quality of Sunn Hemp (Crotalaria Juncea L.) Foliage as Roughage Source for Ruminants by using Microorganisms

2021 ◽  
Vol 18 (24) ◽  
pp. 1440
Author(s):  
Chantira Wongnen ◽  
Krittika Kabploy ◽  
Pijug Summpunn ◽  
Suchat Suksathits
Keyword(s):  
Bacillus Subtilis ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Negative Control ◽  
Gas Production ◽  
Reduction Efficiency ◽  
Sunn Hemp ◽  
Fermented Juice

This study aims to investigate the chemical composition, fermentation characteristics, and in vitro ruminal digestibility efficiency of Sunn hemp silage with Fermented juice of epiphytic lactic acid bacteria (FJLAB) and bacillus subtilis. The experiment was designed in a CRD. Five dietary treatments were fresh Sunn hemp (FS, the positive control), Sunn hemp silage (SS, the negative control), Sunn hemp silage with B. subtilis (SSB), Sunn hemp silage with FJLAB (SSL), and SSB plus FJLAB (SSBL). The results showed the OM content of Sunn hemp silage was decreased (p < 0.05), but fiber contents (NDF, ADF, cellulose, and hemicellulose) were increased when compared with fresh Sunn hemp. However, SSL and SSBL could improve nutrition values (higher CP Reduction efficiency; p < 0.01, decrease cellulose; p < 0.01, and hemicellulose content; p < 0.10) and quality grading of Sunn hemp silage when compare with the negative control which did not affect to CP and EE values. Furthermore, FJLAB reduced fiber content and increase CP content of Sunn hemp silage, whereas B. subtilis presented the opposite results. However, the combination of FJLAB and B. subtilis showed the best treatment of Sunn hemp silage of this experiment (the highest CP and EE Reduction efficiency, ruminal gas production, and organic matter degradability; OMD). HIGHLIGHTS Pyrrolizidine Alkaloids, a toxin associated with disease in ruminants, found in Sunn hemp is completely destroyed by anaerobic microorganisms of silage The fermentation process of silage was improved by increasing lactic acid production and decreasing pH value to inhibit the growth of undesirable microbes Combination of fermented juice of epiphytic lactic acid bacteria (FJLAB) and Bacillus subtilis, lactic acid producer, for Sunn hemp silage treatment yielded the highest lactic acid production and nutritive values GRAPHICAL ABSTRACT

scholarly journals Lactic acid production ability of Lactobacillus sp. from four tropical fruits using their by-products as carbon source

Heliyon ◽  
2021 ◽  
Vol 7 (5) ◽  
pp. e07079
Author(s):  
Joel Romial Ngouénam ◽  
Chancel Hector Momo Kenfack ◽  
Edith Marius Foko Kouam ◽  
Pierre Marie Kaktcham ◽  
Rukesh Maharjan ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Carbon Source ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Tropical Fruits ◽  
By Products

γ-Valerolactone-Introduced Controlled-Isomerization of Glucose for Lactic Acid Production over Sn-Beta Catalyst

2021 ◽  
Author(s):  
Xinpeng Zhao ◽  
Zhimin Zhou ◽  
hu luo ◽  
Yanfei Zhang ◽  
Wang Liu ◽  
...  
Keyword(s):  
Density Functional Theory ◽  
Lactic Acid ◽  
Dft Calculations ◽  
Acid Production ◽  
Density Functional ◽  
Lactic Acid Production ◽  
Hydrothermal Conversion ◽  
Functional Theory ◽  
Environment Friendly

Combined experiments and density functional theory (DFT) calculations provided insights into the role of the environment-friendly γ-valerolactone (GVL) as a solvent in the hydrothermal conversion of glucose into lactic acid...

Impact of storage duration and micro-aerobic conditions on lactic acid production from food waste

2021 ◽  
Vol 323 ◽  
pp. 124618
Author(s):  
Zengshuai Zhang ◽  
Panagiotis Tsapekos ◽  
Merlin Alvarado-Morales ◽  
Irini Angelidaki
Keyword(s):  
Lactic Acid ◽  
Food Waste ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Storage Duration ◽  
Aerobic Conditions
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