scholarly journals Isolasi Senyawa Kimia Stigmastan-3,5-Diena Yang Mempunyai Daya Toksik Dari Daun Ekaliptus (Eucalyptus Deglupta Blume.)

2017 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Sari Setianingsih ◽  
Rudi Kartika ◽  
Partomuan Simanjuntak
Keyword(s):  
Ethyl Acetate ◽  
Toxicity Test ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Test Results ◽  
Phytochemical Screening ◽  
Isolation And Identification ◽  
Lc50 Value ◽  
Ms Analysis

This study was started by extraction of Eucalyptus deglupta Blume. Using organic solvent   (n-hexane, ethyl acetate, ethanol and water) followed by phytochemical screening and toxicity test using Brine Shrimp Lethality Test (BSLT) method. Isolation and identification of chemical compounds contained in the fraction were done by column chromatography and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Phytochemical screening revealed the presence of alkaloids, flavonoids, steroids and phenolics in the extract. Toxicity test results showed that the ethyl acetate extract was potentially active with LC50 value of  617.95 ppm. The extract was continued to isolation stage and gave fraction EKEA-3.1 with LC50 value of 2759.93 ppm. Identification of chemical compounds in EKEA-3.1 with KG-MS analysis showed that EKEA-3.1 was suspected to be Stigmastan-3,5-diene.

Phytochemical screening for identification of bioactive compounds of Lantana camara Linn. (Verbenaceae) responsible for larvicidal action against Aedes triseriatus Say, 1823 (Diptera : Culicidae)

ENTOMON ◽  
2019 ◽  
Vol 44 (2) ◽  
pp. 117-126
Author(s):  
Rhitayu Chakraborti ◽  
Probir Kumar Bandyopadhyay
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Active Ingredient ◽  
Crude Extract ◽  
Ethyl Acetate Extract ◽  
Lantana Camara ◽  
Mosquito Larvae ◽  
Aedes Triseriatus ◽  
Phytochemical Screening ◽  
Lc50 Value

Study to assess the larvicidal property of Lantana camara leaves against Aedes triseriatus larvae found that the ethyl acetate extract had profound larvicidal action with the crude extract having a LC50 value of 409.831ppm. GC-MS analysis of the ethyl acetate extract confirmed the presence of twenty-one compounds out of which beta-caryophyllene covered the highest percentage of the chromatogram area. Further tests with beta-caryophyllene against the mosquito larvae proved it to be the active ingredient of L. Camara with a LC50 value of 104.243ppm.

scholarly journals GC-MS Analysis of Methanol and Ethyl Acetate Extract of fruits of Sphaeranthus indicus

2019 ◽  
Vol 9 (2) ◽  
pp. 28-30
Author(s):  
Kalpana Garg ◽  
Birendra Shrivastava ◽  
Anurag Bhargava
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Sphaeranthus Indicus

Introduction: To identify the various phytoconstituents present in the plant Sphaeranthus indicus by using gas chromatography and mass spectrometry. Meth­ods: The fruits of Sphaeranthus indicus were extracted with Different solvents of increasing polarity. The methanol and ethylacetate extract were subjected to GCMS analysis to detect the phytoconstituents. Results: Totally 26 compounds were identified. Among these 13 constituents in methanol extract and 13 constituents in ethylacetate extract were identified during the GC-MS analysis. Stigmasterol and lupeol which were identified in the plant is considered to have antiarthritic properties. Keywords: Sphaeranthus indicus, Gas chromatography, Mass spectrometry.

scholarly journals Identifikasi Senyawa Kimia Fenolik Dalam Ekstrak Etil Asetat Kulit Buah Jengkol (Archidendron pauciflorum (Benth.) I.C Nielsen)

2019 ◽  
Vol 11 (02) ◽  
pp. 111-119
Author(s):  
Ahmad Sopian Pian
Keyword(s):  
Mass Spectrometry ◽  
Ethyl Acetate ◽  
Reduction Method ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Processed Food ◽  
Fruit Peel ◽  
Fruit Skin ◽  
Radical Reduction

Jengkol (Archidendron pauciflorum (Benth.) I.C Nielsen) is a plant that is already familiar inIndonesia and is widely used as a processed food that is quite popular. One of the underutilized parts ofthe jengkol plant is the skin. Jengkol fruit peel contains alkaloid compounds, flavonoids, tannins,glycosides, sapoinin and steroids or triterpenoids. The purpose of this study was to determine the contentof chemical compounds in ethyl acetate extract of jengkol fruit peel, and to test antioxidant activity byDPPH free radical reduction method. The results of isolation, purification in ethyl acetate extract ofjengkol fruit skin does not provide activity as an antioxidant. The results of the analysis using FTIRspectrophotometry, Gas chromatography-Mass Spectrometry (GC-MS) and Nuclear Magnetic ResonanceSpectroscopy ( 1 HNMR).

scholarly journals EVALUATION OF ANTIOXIDANT, ANTIBACTERIAL, AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHYL ACETATE EXTRACT OF STREPTOMYCES OMIYAENSIS SCH2

2018 ◽  
Vol 11 (7) ◽  
pp. 271 ◽  
Author(s):  
Janpen Tangjitjaroenkun
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Antioxidant Power ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis

Objective: The aim of this study was to evaluate the antibacterial and antioxidant properties of ethyl acetate extract of Streptomyces omiyaensis SCH2. The chemical constituents of the extract were investigated using gas chromatography-mass spectrometry (GC-MS).Methods: Secondary metabolites from S. omiyaensis were produced by submerge fermentation using ISP2 medium with 3% NaCl (w/v) for 21 days. The culture filtrate was extracted with ethyl acetate. The chemical constituents were detected in the GC-MS analysis. Antibacterial activity was performed using disc diffusion and broth microdilution methods. Antioxidant activity was evaluated by determining the reducing power capacity and free radical scavenging assays.Results: The GC-MS analysis of the SCH2 extract revealed the presence of four compounds. The main constituents were 2-phenylacetamide (79%). The extract exhibited the highest zone of inhibition against some pathogenic bacteria such as Enterobacter cloacae, Klebsiella pneumoniae, and Bacillus subtilis. In addition, the lowest minimum inhibitory concentration (IC) and minimum bactericidal concentration values of extract were obtained for E. cloacae (0.125 and 4 mg/ml). The extract showed antioxidant potential with IC50 values of 2.078.13±24.58 μg/ml and 475.74±4.56 μg/ml for 2,2-diphenyl-1-picryl-hydrazyl and 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid] assays, respectively. The ferric reducing antioxidant power value of extract was 110.36±1.75 mmol FeSO4.7H2O/g extract.Conclusion: This study indicated that S. omiyaensis extract possesses antibacterial and antioxidant activities. GC-MS analysis revealed the presence of major chemical constituents, acetamide, and pyrrolopyrazine which could be responsible for the biological activities. S. omiyaensis extract could be used as a potential of natural antibacterial and antioxidant agents for pharmaceutical and medical applications.

scholarly journals Skrining Fitokimia Ekstrak Etanol, Etil Asetat Dan N-Heksana Batang myristica Fragrans

2019 ◽  
Vol 2 (1) ◽  
pp. 97-100
Author(s):  
Rizki Damayanti ◽  
Ria Ervilita
Keyword(s):  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Screening Test ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Hexane Extract ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Myristica Fragrans

Telah dilakukan uji skrining fitokimia terhadap batang Myristica fragrans. Uji fitokimia yang dilakukan diantaranya adalah alkaloid, flavonoid, fenolik, saponin, dan terpenoid/ steroid. Hasil uji skrining fitokimia ekstrak etanol batangMyristica fragrans menunjukkan adanya kandungan flavonoid, saponin dan terpenoid/ steroid. Hasil skrining fitokimia pada ekstrak etil asetat menunjukkan adanya falvonoid dan terpenoid/ steroid sedangkan pada ekstrak n-heksana menujukkan adanya senyawa terpenoid/ steroid. Senyawa-senyawa kimia yang tidak terdapat pada ketiga ekstrak dengan variasi pelarut daun Myristica fragrans adalah senyawa alkaloid dan fenolik.   Phytochemical screening tests on the stem of Myristica fragrans have been carried out. Phytochemical tests were included alkaloids, flavonoids, phenolics, saponins, and terpenoids/steroids. The results of the phytochemical screening test of ethanol extract from the stem of Myristica fragrans showed the presence of flavonoids, saponins and terpenoids/steroids. The results of phytochemical screening on ethyl acetate extract showed phalvonoid and terpenoids/steroids whereas n-hexane extract showed terpenoids/steroids. Chemical compounds which were not found in the three extracts with a variety of solvent leaves Myristica fragrans were alkaloid and phenolic compounds.

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAN SKRINING FITOKIMIA EKSTRAK HEKSANA, ETIL ASETAT DAN METANOL TUMBUHAN SURUHAN (Peperomia pellucida L. Kunth)

2020 ◽  
Vol 16 (2) ◽  
pp. 105-111
Author(s):  
Restianti Restianti ◽  
Bimo Budi Santoso ◽  
Maria Ludya Pulung
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Hexane Extract ◽  
Phytochemical Screening ◽  
E Coli ◽  
Activity Test ◽  
Solvent Analysis

Antibacterial activity test and screening of phytochemical of extract hexane, ethyl acetate  and methanol have been carried out. The extraction of the P.pellucida plants was carried out by the maceration method in stages based on the polarity of the solvent. Analysis of chemical compounds done with phytochemical screening test. Based on the identification of chemical compounds by phytochemical screening, hexane and ethyl acetate extract has been detected only in the presence of flavonoid compounds with moderate categories. Methanol  extract contains high-grade alkaloids and moderate flavonoid and tannin compounds. Antibacterial test using disffusion methods suggest that ethyl acetate and methanol extract have categorically strong antibacterial activity againts the S. aureus  of 14 mm and 16 mm respectively. Hexane extract and acetate ethyl have a strong inhibition to  E. coli  of 11 mm and 12 mm respectively. Methanol extract has a weak inhibition to bacteria E. coli  and a hexane extract has a weak inhibition to bacteria S. aureus.

scholarly journals Isolasi dan Identifikasi Steroid dari Ekstrak N-Heksana Daun Afrika (Vernonia amygdalina Del.)

KOVALEN ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 18-22
Author(s):  
Evi Mashunah ◽  
Erwin ◽  
Saibun Sitorus
Keyword(s):  
Data Analysis ◽  
Toxicity Test ◽  
Brine Shrimp ◽  
Test Results ◽  
Separation And Purification ◽  
Vernonia Amygdalina ◽  
Isolation And Identification ◽  
Brine Shrimp Lethality ◽  
Lc50 Value ◽  
Ft Ir

Isolation and identification of steroids from the n-hexane fraction of Vernonia amygdalina Del. was carried out. Separation and purification were performed using flash chromatographic and gravity chromatography. Based on the results of phytochemical tests supported by UV and FT-IR data analysis, the isolate obtained was a steroid (sterol type). Toxicity test results using the Brine Shrimp Lethality Test (BSLT) were known that the steroid has moderate toxicity with an LC50 value of 48.39 ppm. Keywords: Vernonia amygdalina Del., Toxicity, BSLT, Sterol, Steroid

scholarly journals EFIKASI GRANUL BIOLARVASIDA NYAMUK AEDES AEGIPTY DARI EKSTRAK ETIL ASETAT DAUN BELUNTAS

2017 ◽  
Vol 22 (1) ◽  
pp. 15
Author(s):  
Agus Rochmat ◽  
Mita Napitasari ◽  
Afdwiyarni Metta Karina
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Quinic Acid ◽  
Mosquito Larvae ◽  
Extract Concentration ◽  
Lc50 Value ◽  
Ms Analysis ◽  
Extract Content ◽  
Positive Controls

Penelitian ini bertujuan untuk mengetahui aktivitas dari ekstrak daun beluntas terhadap mortalitas larva nyamuk Aedes aegypti. Ekstrak daun beluntas diperoleh dengan menggunakan metode ekstraksi pada pelarut etanol. Setelah didapatkan ekstrak kental, maka ekstrak tersebut difraksinasi dengan pelarut n-heksana dan etil asetat. Kemampuan biolarvasida ekstrak daun beluntas ditentukan melalui nilai LC50 dan diperkuat dengan identifikasi kandungan senyawa aktif. Uji biolarvasida ini dilakukan terhadap larva nyamuk Aedes aegypti dengan variasi konsentrasi ekstrak 50, 100, 250, 500, dan 1000 ppm selama 24 jam pengamatan. Identifikasi kandungan senyawa dilakukan dengan pengujian sampel dengan analisa GC-MS. Hasil penelitian menjunjukkan bahwa rendemen ekstrak etil asetat sebesar 1,86 %. Nilai LC50 ekstrak etil asetat ini adalah 105,79 ppm. Nilai LC50 yang diperoleh termasuk golongan biolarvasida aktif dan kontrol positif menggunakan abate memiliki nilai LC100 pada konsentrasi 100 ppm. Sementara itu, nilai LC50 pada granul dengan kandungan ektrak daun beluntas yang terbaik diperoleh nilai 96,34 ppm dan nilai LC90 adalah 905.1 ppm. Kemampuan biolarvasida aktif ekstrak beluntas ini dikuatkan dengan hasil analisa GC-MS yang menunjukkan bahwa kandungan senyawa aktif biolarvasida yang terkandung dalam ekstrak etil asetat diduga asam quanat.(THE EFFICATION OF AEDES AEGYPTI BIOLARVASIDA GRANUL FROM ETIL ACETAT EXTRACT OF MARSH FLEABANE LEAVES)This study was aimed at determining the activity of Marsh Fleabane leaves extract against Aedes aegypti mosquito larvae mortality. Marsh Fleabane leaves extract was obtained using ethanol solvent. After obtaining the viscous extract, the extract was fractionated with solvent n-hexan and ethyl acetate. The ability of this biolarvaside was determined by LC50 value and reinforced by identifying of active compound content. This biolarvaside test was conducted on Aedes aegypti mosquito larvae with variation of extract concentration 50, 100, 250, 500, and 1000 ppm during 24 hours observation. Identification of compound content was done by testing the sample with GC-MS analysis. The results show that the yield of ethyl acetate extract equal to 1.86%. The LC value of this ethyl acetate extract was 105.79 ppm. The obtained LC50 values including active biolarvacidal groups and positive controls using abate had LC100 values at 100 ppm concentrations. Meanwhile, the LC50 value on granules using marsh Fleabane leaves extract content was obtained at 96.34ppm and LC90 was 905.1 ppm. The ability of active biolarvaside is corroborated by GC-MS analysis results indicating that the biolarvacid content of active compounds contained in ethyl acetate extract expected as quinic acid

In-vitro bactericidal activity of a novel plant source Plumeria pudica against some human and fish pathogenic bacteria

2020 ◽  
Vol 17 ◽  
Author(s):  
Shubhaisi Das ◽  
Sunanda Burman ◽  
Goutam Chandra
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Ft Ir ◽  
Aldehyde Groups

Background: The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. Objective: The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Methods: Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Results: Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Conclusion: Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.

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