scholarly journals Uji Fitokimia dan Toksisitas Ekstrak Etil Asetat Kulit Batang Melochia umbellata (Houtt) Stapf. var. Visenia dengan Metode Brine Shirmp Lethality Test (BSLT)

2017 ◽  
Vol 4 (2) ◽  
pp. 378-381 ◽  
Author(s):  
Fauziah Ahmad ◽  
Nunuk H. Soekamto ◽  
Firdaus Firdaus
Keyword(s):  
Ethyl Acetate ◽  
Toxicity Test ◽  
Ethyl Acetate Extract ◽  
Artemia Salina ◽  
Bark Extract ◽  
Steroid Compound ◽  
Value Analysis ◽  
South Sulawesi ◽  
The People ◽  
Test Result

Melochia umbellata (Houtt) Stapf. var. Visenia is plant species included in Malvaceae family. This spesies is known as paliasa and used as traditional medicine by the people of South Sulawesi. This study aimed to determine the secondary metabolites by reagents and toxicity characteristic testing from bark extract of Melochia umbellata (Houtt) Stapf. var. Visenia using Brine Shirmp Lethality Test (BSLT) method. The extract was prepeared by maceration ethyl acetate. Phytochemical test result showed that the ethyl acetate extract was containing the steroid compound, alkaloids and terpenoids. Toxicity test performed by shrimp Artemia salina Leach larvae was at 48 hours. The toxic effects of the extract were identified by the percentage of the number of shrimp larvae mortality using probit value analysis (LC50). The toxicity test of bark Melochia umbellata (Houtt) Stapf. var. Visenia extract showed that the ethyl acetate extract is toxic to A. salina  LC50 = 54,55 mg / mL.

scholarly journals Antimicrobial and larvicidal potential of endophytic fungi isolated from Tragia involucrata Linn.

2016 ◽  
Vol 6 (01) ◽  
pp. 1494 ◽  
Author(s):  
Ananda Danagoudar ◽  
Chandrashekhar G. Joshi* ◽  
M.T. Nivya ◽  
H.M. Manjunath ◽  
Jagadeesha Poyya ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Larvicidal Activity ◽  
Endophytic Fungi ◽  
Ethyl Acetate Extract ◽  
Penicillium Citrinum ◽  
Diffusion Method ◽  
Disc Diffusion Method ◽  
The People ◽  
Therapeutic Compounds

Endophytic fungi are one of the untapped resources of therapeutic compounds for various diseases. The present study focused on the antimicrobial as well as larvicidal activity of ethyl acetate extract of endophytic fungi isolated from Tragia involucrata. The ethyl acetate extract of Penicillium citrinum CGJ-C1 (GenBank No.KT780618), Penicillium citrinum CGJ-C2 (KP739821), Cladosporium sp. (KP739822), and Cryptendoxyla hypophloia CGJ-D2 (KT780619) was subjected to antimicrobial activity against a panel of microorganisms by disc diffusion method, larvicidal activity against Culex quinquefasciatus. All the extracts showed significant antimicrobial activity against the tested organisms ranging from 8±0.32 to 13±2.11. The extent of activity was comparable to the standard drugs. The larvicidal potential of the endophytes was superior to T.involucrata extract. The larvicidal activity was found to be dose and time dependent with LC50 value ranging from 4.25- 158.06ppm after 24hrs of treatment. This is the first report on the bioactivity of the endophytes isolated from T.involucrata. Further studies on the bio-guided isolation of lead compound will benefit the people suffering from microbe’s related diseases.

scholarly journals KLT BIOAUTOGRAFI HASIL PARTISI EKSTRAK ETANOL HERBA BANDOTAN (Ageratum conyzoides L.) TERHADAP Shigella dysentriae

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Surya Sumantri Abdullah ◽  
Natsir Djide ◽  
Sartini Natsir
Keyword(s):  
Ethyl Acetate ◽  
Incubation Time ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Diameter Measurement ◽  
Steroid Compound ◽  
Ageratum Conyzoides ◽  
Bioassay Method

Penelitian uji daya hambat dan analisis KLT bioautografi hasil partisi ekstrak etanol herba bandotan (Ageratum conyzoides L.) terhadap Shigella dysentriae  telah dilakukan. Penelitian ini bertujuan untuk mengetahui kemampuan ekstrak tersebut dalam menghambat pertumbuhan S.dysentriae dan membandingkan daya hambat ekstrak dengan tingkat kepolaran yang berbeda yaitu pada ekstrak n-heksana, larut etil asetat, dan tidak larut etil asetat berdasarkan  pengukurandiameter hambatan yang terbentuk. Herba tersebut diekstraksi dengan etanol menggunakan metode maserasi, lalu dipartisi dengan pelarut n-heksana dan etil asetat. Pengukuran dilakukan dengan menggunakan metode difusi pada medium Miller Hinton Agar (MHA) dengan waktu inkubasi 24 jam pada suhu 37oC dan memberikan diameter daerah hambatan terbesar pada ekstrak tidak larut etil asetat herba bandotan yaitu 9,3 dan 10,3 mm. Pemisahan secara KLT pada ekstrak n-heksana, ekstrak etil asetat, dan ekstrak tidak larut etil asetat dengan cairan pengelusi berturut-turut, n-heksana:etil asetat (3:1), n-heksana:etil asetat (1:1), dan etil asetat:etanol (10:1) dengan jumlah bercak noda berturut-turut 4, 5, dan 2. Nilai Rf pada ekstrak n-heksana 0.25, 0.41, 0.52, dan 0.71 sedangkan pada ekstrak etil asetat 0.34, 0.53, 0.65, 0.81, dan 0.92 pada ekstrak tidak larut etil asetat 0.33 dan 0.64. Hasil KLT bioautografi diperoleh komponen antibakteri yang diidentifikasi ekstrak n-heksana adalah golongan steroid dan pada tidak larut etil asetat golongan polifenol ABSTRACTInhibition test research and TLC bioautographic bioassay method of the partition results of the ethanol extract of bandotan (Ageratum conyzoides L.) herb against Shigella dysentriae have been conducted. This study aims to determine the ability of these extracts to inhibit S.dysentriae growth and to compare the inhibition of extracts with different polarity levels, n-hexane soluble, ethyl acetate soluble, and ethyl acetate insoluble extracts based on the diameter measurement of the formed resistance. The herbs were extracted with ethanol using the maceration method, then partitioned with n-hexane and ethyl acetate solvents. Measurements were carried out using the diffusion method on Miller Hinton Agar (MHA) medium with an incubation time of 24 hours at 37oC and gave the largest diameter area of resistance to the ethyl acetate insoluble extract of bandotan herb, value 9.3 and 10.3 mm. Separation by TLC on n-hexane extract, ethyl acetate extract, and ethyl acetate insoluble extract with elusive liquid respectively, n-hexane: ethyl acetate (3: 1), n-hexane: ethyl acetate (1: 1), and ethyl acetate: ethanol (10: 1) with the number of stains 4, 5, and 2, respectively. 0.81, and 0.92 in ethyl acetate insoluble extracts 0.33 and 0.64. The results of the bioautography TLC bioassay method showed that the antibacterial component identified in the n-hexane extract was a steroid compound and ethyl acetate insoluble was a polyphenol compound.

scholarly journals Isolasi Senyawa Kimia Stigmastan-3,5-Diena Yang Mempunyai Daya Toksik Dari Daun Ekaliptus (Eucalyptus Deglupta Blume.)

2017 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Sari Setianingsih ◽  
Rudi Kartika ◽  
Partomuan Simanjuntak
Keyword(s):  
Ethyl Acetate ◽  
Toxicity Test ◽  
Ethyl Acetate Extract ◽  
Chemical Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Test Results ◽  
Phytochemical Screening ◽  
Isolation And Identification ◽  
Lc50 Value ◽  
Ms Analysis

This study was started by extraction of Eucalyptus deglupta Blume. Using organic solvent   (n-hexane, ethyl acetate, ethanol and water) followed by phytochemical screening and toxicity test using Brine Shrimp Lethality Test (BSLT) method. Isolation and identification of chemical compounds contained in the fraction were done by column chromatography and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Phytochemical screening revealed the presence of alkaloids, flavonoids, steroids and phenolics in the extract. Toxicity test results showed that the ethyl acetate extract was potentially active with LC50 value of  617.95 ppm. The extract was continued to isolation stage and gave fraction EKEA-3.1 with LC50 value of 2759.93 ppm. Identification of chemical compounds in EKEA-3.1 with KG-MS analysis showed that EKEA-3.1 was suspected to be Stigmastan-3,5-diene.

scholarly journals Profil Fraksi Sitotoksik terhadap Sel Murine Leukemia P-388 dari Ekstrak Biji Honje (Etlingera elatior)

2017 ◽  
Vol 3 (1) ◽  
pp. 79-87
Author(s):  
Alfindah Rusanti ◽  
Dede Sukandar ◽  
Tarso Rudiana ◽  
Adawiah Adawiah
Keyword(s):  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Mtt Assay ◽  
Chemical Structure ◽  
Ethyl Acetate Extract ◽  
Artemia Salina ◽  
Cytotoxic Compounds ◽  
Murine Leukemia ◽  
Etlingera Elatior

The research characterization of cytotoxic fraction against P-388 leukemia murine cells from the extract honje (Etlingera elatior) seed have been reported. This research lead to isolated and characterization of cytotoxic compounds against P-388 leukemia murine cells from the extract E. elantior seed. The extract of E. elantior seed was maserated by methanol, n-hexane, and ethyl acetate, respectively and estimated their cytotoxic activity against P-388 leukemia murine cell with 3- (4, 5-dimetiltiazol-2-yl) -2,5-difeniltetrazolium bromide (MTT) assay guided toxicity test against of shrimp Artemia salina Leach. Brine shirmp Lethality Test (BSLT) method. The active extracts will be separated by fractionation using column chromatography, radial chromatography, and for analyzing the purity of isolate will estimate by HPLC. The chemical structure of pure isolate will be identified by spectroscopies data UV Vis, FTIR, NMR and MS. The ethyl acetate extract from honje seed have cytotoxic activity by leukemia P-388 cell  with IC50 19.21 µg/mL. The compound toxic as cytotoxicagainst P-388 leukemia murine cells is flavonoid compouds their is resveratrol, lapachol, apigenin, methylated chrysin, 6,2’-dihydroxyflavanone, 3-hydroxy-3,4’-dymethoxyflavone and 4’-hydroxy-5,7-dimethoxyflavanone.DOI: http://dx.doi.org/10.15408/jkv.v0i0.3640

scholarly journals The Antioxcidant, Antibacterial Activity and Toxicity of Kesambi (Schleichera Oleosa (Lour) Oken) Brak Extracts

2020 ◽  
Vol 4 (3) ◽  
Author(s):  
Puspita Sari
Keyword(s):  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Toxicity Test ◽  
Ethyl Acetate Extract ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Toxicity Tests ◽  
Agar Diffusion ◽  
Schleichera Oleosa

Abstract This study aimed to compare antioxidant, antibacterial and toxicity tests of various types of kesambi tree bark extract (Schleichera oleosa (Lour) Oken). The research stages included extraction of kesambi stem skin from Mancak Serang sub-district with multilevel maceration techniques using 3 solvents: Methanol, n-hexane, and ethyl acetate. The extract was then tested for antioxidant activity, antibacterial, and toxicity. The results of antioxidant testing using the DPPH method, antibacterial with agar diffusion method, and toxicity test with BSLT method, the results showed that ethyl acetate extract had better antioxidant activity (IC50 7,723 ppm.) compared to extract of methanol (IC50 7,801 ppm), and n-hexane extract (IC50 8,568 ppm). Antibacterial activity showed the ability to inhibit the growth of Streptococcus aureus compared to Escherichia coli at a concentration of 10000 ppm, and the results of the toxicity test showed that ethyl acetate extract had better activity (LC50 305,17 ppm) than n-hexane extract (LC50 374, 96 ppm) and methanol extract (LC50 431,26 ppm).Keywords: DPPH, BSLT, Agar Diffusion, Schleichera oleosa (Lour) Oken

scholarly journals IDENTIFIKASI SENYAWA FLAVONOID PADA FRAKSI ETIL ASETAT BENALU BATU (Begonia sp.) ASAL KABUPATEN MOROWALI UTARA

2016 ◽  
Vol 2 (2) ◽  
pp. 83-89
Author(s):  
Agus Ritna ◽  
Syariful Anam ◽  
Akhmad Khumaidi
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Preparative Thin Layer Chromatography ◽  
Ethyl Acetate Fraction ◽  
Visible Spectroscopy ◽  
Flavonoid Compounds ◽  
The People ◽  
Color Reagent ◽  
Vacuum Liquid Chromatography ◽  
Layer Chromatography

Benalu Batu Plant (Begonia sp.) in family Begoniaceae is a plant used by the people of North Morowali to treat tumors and cancers. It generally contains saponins, tannins, flavonoids and polyphenols. This research aimed to identify flavonoid compounds contained in ethyl acetate fraction of Begonia sp. The simplicia was extracted using maceration method and partitioned using n-hexane, ethyl acetate and water. The Ethyl acetate extract was then fractionated by nine eluent combinations using separation method of Vacuum Liquid Chromatography (VLC) and Preparative Thin Layer Chromatography (Prep TLC). One fraction was obtained and was suspected to contain flavonoid compounds after Shinode and Pew color reagent test. In the result of Ultraviolet-Visible Spectroscopy with methanol at wavelength range of 200-550 nm, the isolates showed absorption peaks at 275 nm (peak 1) and 225 nm (peak 2). Based on the wavelength of flavonoid compounds contained in the fraction, Begonia sp. showed similarities to the absorption peak of flavan-3-ol or flavanols

scholarly journals Toxicity Extract and Faction of Surian Toona sinensis Leaf and Bark against Shrimp Larvae Artemia salina L.

2019 ◽  
Vol 3 (3) ◽  
pp. 128-137
Author(s):  
Rori Theresia ◽  
Syamsul Falah ◽  
Mega Safithri ◽  
Muhammad Assyar
Keyword(s):  
Ethyl Acetate ◽  
Water Content ◽  
Diethyl Ether ◽  
Water Extract ◽  
Artemia Salina ◽  
Probit Analysis ◽  
Bark Extract ◽  
Toona Sinensis ◽  
Lc50 Value ◽  
Toxic Concentration

Surian (Toona sinensis) is one of the herbs that contain many bioactive compounds. Part of surian plants that can be utilized as therapy is leaf and bark. This study aims to determine the toxicity of leaf and bark extract of Surian through Bhrine Shrimp Lethality Test (BSLT), then compare toxic concentration of water extract, and ethanol 70 %, and n-hexane, diethyl ether and ethyl acetate fractions to shrimp larvae (Artemia salina L). The results showed that water content of leaves simplicia was 8.08 % and bark was 6.26 %. The highest extract rendement value by ethanol solvent was 23 %. The result of BSLT test probit analysis showed that the extract of ethyl acetate bark fraction had the highest toxicity with LC50 value 405.641 ppm, while leaf water extract had the lowest toxicity with LC50 value 707.787 ppm.

scholarly journals Screening for Bioactive Extracts and Targeted Isolation of Antimicrobial Agents from the Stem Bark of Adenanthera pavonina L.

2020 ◽  
pp. 227-242
Author(s):  
K. Abdu ◽  
M. Adamu
Keyword(s):  
Ethyl Acetate ◽  
Antimicrobial Agents ◽  
Ethyl Acetate Extract ◽  
Scientific Evidence ◽  
Bacterial Species ◽  
Stem Bark ◽  
Artemia Salina ◽  
Scientific Data ◽  
Adenanthera Pavonina ◽  
Solvent Extract

Bioactive secondary metabolites can be isolated from medicinal plants as antimicrobial agents. Based on ethnopharmacological relevance, Adenanthera pavonina L. is recognized as a plant with good medicinal values and forms the integral part systems of traditional medicine in Borno state, and other parts of Nigeria. Despite the widespread used, most of the information available to consumers about the plant is not backed by credible scientific data. Only a limited number of studies have probed into the scientific evidence for bioactivity and safety of this medicinal plant. Such studies rarely progress to isolation and evaluation of the active antimicrobial agents. In the present study, four solvent-extract (ethanol, ethyl acetate, chloroform and n-hexane) were subjected to antimicrobial activity test against E. coli (E.C), S. typhi (S.T), S. aureus (S.A), A. flavus (A.F), C. albican (C.A) and M. specie (M.S) and toxicity test against Artemia salina. The ethyl acetate extract showed outstanding activity against the microbes (bacteria and fungi) and Artemia salina, respectively. The activity and toxicity effects of the ethyl acetate extract suggests the presence of active antimicrobial agents and hence provides a way forward for column chromatographic isolation of the targeted Antimicrobial agents. The targeted compounds isolated (AP-X38 and AP-X44) were also subjected to antimicrobial test using three bacterial species and found to be even more active that the solvent extract of ethyl acetate. This study justifies part of ethno medicinal claims on the plant.

scholarly journals ANTIOXIDANT ACTIVITY OF ETHYL ACETATE EXTRACT OF NUTMEG (Myristica fragrans Houtt) STEM BARK

2019 ◽  
Vol 19 (3) ◽  
pp. 58-63
Author(s):  
Ilham Maulana ◽  
Binawati Ginting ◽  
Nurdin Nurdin ◽  
Saiful Fakri
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Bark Extract ◽  
Test Results ◽  
Myristica Fragrans ◽  
Ic50 Value ◽  
Stem Bark Extract ◽  
Myristica Fragrans Houtt

Antioxidant activity test for the extract of ethyl acetate nutmeg stem bark (Myristica fragrans Houtt) was carried out using 2,2-diphenyl-1picrylhydrazil (DPPH). The test results of antioxidant activity of ethyl acetate nutmeg stem bark extract with DPPH method at a concentration of 25 ppm, 50 ppm and 100 ppm obtained IC50 value = 68.14 ppm with IC50 value of vitamin C (as positive control) 3.657 ppm. The results of column chromatographic separation of ethyl acetate nutmeg stem bark extract obtained 5 combined fractions namely MFEK 1, MFEK 2, MFEK 3, MFEK 4 and MFEK 5. The test results of the fraction antioxidant activity in a good category were the MFEK 1 fraction. The MFEK 2 fraction to MFEK 5 showed an antioxidant activity lower than compared  to the ethyl acetate extract. This shows that the ethyl acetate nutmeg stem bark extract has a better inhibitory activity, because there are several active compounds contained in the extract which can inhibit free radicals, so the IC50 value of the extract is better than the fractions.

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