scholarly journals Induksi mutasi kedelai (Glycine max (L.) Merrill) dengan sodium azida pada tanah salin

2019 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Nurul Fajriyah ◽  
Karno Karno ◽  
Florentina Kusmiyati

ABSTRACT  Mutation is one of plant breeding ways to expand genetic diversity. The purpose of the research was to evaluate the effect of sodium azide mutagen on soybean variety Dering 1 at saline and non-saline soil. The research was arranged in Factorial Design based on Completely Randomized Design with 2 factors. The first factor was doses of Sodium Azide consisted of 0 mM, 0.05 mM, 0.1 mM, 0.2 mM, dan 0.4 mM, 0.8 mM, 1.6 mM, 3.2 mM, 6.4 mM, 12.8 mM, and 25.6 mM. The second factor was salinity levels consisted of 0 dS/m, 2 dS/m and 5 dS/m. Parameters measured were plant height, number of leaves, number of pods, weight of pod, number of seeds, and weight of seeds per plant on M1 generation. Result showed that lethal dosage (LD) 50 was obtained at 0,663 mM. Sodium azide mutagent caused diversity of plant height, number of leaves at saline and non-saline soil. There was 10 plants and 3 plants that was classified as tolerant and most tolerant at saline soil (2 dS/m) respectively. Keywords : soybean, sodium azide, saline soil ABSTRAK  Mutasi adalah salah satu cara pemuliaan tanaman untuk memperluas kergaman genetik. Tujuan penelitian untuk mengevaluasi pengaruh mutagen sodium azida terhadap kedelai varietas Dering 1 pada tanah salin dan non-salin. Rancangan percobaan yang digunakan di greenhouse adalah Percobaan Faktorial dengan dasar Rancangan Acak Lengkap yang terdiri dari 2 faktor. Faktor pertama adalah dosis mutagen kimia Sodium Azide (SA) yang terdiri dari 11 taraf perlakuan yaitu 0 mM, 0,05 mM, 0,1 mM, 0,2 mM, dan 0,4 mM, 0,8 mM, 1,6 mM, 3,2 mM, 6,4 mM, 12,8 mM, 25,6 mM. Faktor kedua adalah tingkat salinitas yaitu 0 dS/m, 2 dS/m dan 5 dS/m. Parameter yang diamati adalah tinggi tanaman, jumlah daun, jumlah polong, berat polong, jumlah biji, dan berat biji per tanaman pada generasi M1. Hasil penlitian menunjukkan bahwa dosis letal median (LD50) diperoleh pada 0,663 mM. Mutagen sodium azida menyebabkan keragaman tinggi tanaman, jumlah daun pada tanah salin dan non-salin. Terdapat 10 tanaman dan 3 tanaman yang masing-masing tergolong tahan dan sangat tahan pada tanah salin (2 dS/m). Kata kunci : kedelai, sodium azida, tanah salin

Author(s):  
Budiman Budiman ◽  
Natsir Sandiah ◽  
La Malesi

This study aimed to determine the best dosage of goat manure as a natural fertilizer on Beha grass(Brachiaria humidicola). This study used 64 poles of Beha grass grown in polybags divided into 16plots, and it used a completely randomized design (CRD) to analyze the data. This study consist of 4treatments that is P0 (0 ton/ha of goat manure), P1 (10 ton/ha of goat manure), P2 (15 ton/ha of goatmanure), and P3 (20 ton/ha of goat manure). This study's variables were plant height, number of leaves,numbers of tillers, new production, and root length. Data analysis used ANOVA variance continued withreal honest difference test. The result of this study showed that averages of plant height (cm) wereP0=26, P1=50.5, P2=51.5, and P3=52.75. Averages of the number of leaves were P0=22, P1=110,P2=160, and P3=185. Averages of numbers of tillers were P0=7, P1=25, P2=37, and P3=38. Averages ofnew production (g) were P0=17.75, P1=123.5, P2=178, and P3=192.25. Averages of root length (cm)were P0=37.8, P1=39.5, P2=41.775, and P3=49.5. Fertilization with natural fertilizer from goat manurehas a genuine effect (P<0.01) on the growth of Beha grass. The best dosage of fertilization with goatmanure was 15 ton/ha.


2019 ◽  
Vol 15 (2) ◽  
pp. 105-114
Author(s):  
GUSTI MARLINA ◽  
MARLINDA MARLINDA ◽  
HENI ROSNETI

The research objective was determine the effect of various growth media and growmore fertilizer on the acclimatization of Dendrobium orchids. study used a Completely Randomized Design (CRD) Factorial consisting of 2 factors, the first is (M) of growing media which consists of 3 levels, namely: M1 (medium fern root), M2 (wood charcoal medium), M3 (coir media coconut) and the second is (G) growmore fertilizer which consists of 4 levels, namely: G0 (without Growmore fertilizer), G1 (Growmore fertilizer 2 g / liter), G2 (fertilizer Growmore 4 g / liter), G3 (Growmore fertilizer 6 g / liter). Determine the growth of orchid plants, observations were made with the following parameters Growth Percentage (%), Plant Height (cm), Number of leaves (strands), and Number of tillers (stems). Data obtained were analyzed statistically, then continued with the Honestly Significant Difference Test (BNJ) at the level of 5%. The results showed that the single treatment of the use of growing media did not have a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). The growth of growmore fertilizer also did not show a significant effect on the parameters of observation of growth percentage (%), plant height (cm), number of leaves (strands), and number of tillers (stems). Interaction of the use of various growth media and growmore fertilizer did not show a significant effect on the percentage of growth (%), plant height (cm), number of leaves (strands), and number of tillers (stems).


2020 ◽  
Vol 7 (1) ◽  
pp. 43-48
Author(s):  
Eduardo Santana Aires ◽  
Carlos Alberto Aragão ◽  
Itala Laiane Silva Gomes ◽  
Gilmário Noberto de Souza ◽  
Isa Gabriela Vieira de Andrade

This study aimed to evaluate different substrates to produce yellow passion fruit seedlings. For this purpose, an experiment was carried out in a greenhouse, from April to June 2016. A completely randomized design with five replications, in a 6 x 5 factorial scheme was used. The first factor was the alternative substrates (poultry, cattle and equine manure, sugarcane and coconut bagasse, and Organomais compost). The second factor was the replacement levels to the commercial substrate of Plantmax® (100:0, 75:25, 50:50, 25:75, 0:100). The seedling evaluation was carried out 42 days after sowing. The following variables were analyzed: length, width, and the number of leaves; plant height and shoot dry matter. A significant effect (P0.05) for the interaction between the factors was verified. The addition of 60% cattle manure and Organomais to the commercial substrate Plantmax® promoted greater length, width, and the number of leaves, plant height, and shoot dry matter accumulation in the yellow passion fruit seedlings.


2018 ◽  
Vol 7 (1) ◽  
pp. 6
Author(s):  
Hairin Juanda ◽  
Tutik Nugrahini ◽  
Mahdalena Mahdalena

Effect of NASA Liquid Organic Fertilizer and Compost Fertilizer on Kenaf Plant Growth (Hibiscus cannabinus L). This study aims to determine the effect of NASA POC, compost fertilizer and the interaction of NASA POC and compost fertilizer on kenaf plants. This research was carried out at East Borneo Samarinda Indonesia. with a time of ± 4 months, starting from June to September 2016. The design of the study uses factorial completely randomized design (CRD) consisting of 2 treatments and 4 replications. The treatment consists of 2 factors. The first factor is the administration of NASA POC consisting of 4 levels, namely: j0: Control (without treatment), j1: POC with a concentration of 10 ml / liter of water, j2: POC with a concentration of 15 ml / liter of water, j3: POC with a concentration of 20 ml / liter of water. The second factor is Compost fertilizer consisting of 4 levels, namely: k0: Without the provision of Compost Fertilizer, k1: Composting 500 gram / polybag, k2: Composting Fertilizer 700 grams / polybag, k3: Composting 800 grams / polybag. From the analysis of variance showed that the effect of giving NASA POC (J), compost (K) and the interaction of NASA POC and compost (JK) did not affect the parameters of plant height 30 DAP, 60 DAP and 90 DAP, stem diameter 30 DAP, 60 DAP and 90 DAP, number of leaves 30 DAP, 60 DAP and 90 DAP, leaf area 30 DAP, 60 DAP and 90 DAP. 


2018 ◽  
Vol 23 (1) ◽  
pp. 51 ◽  
Author(s):  
Tintin Suhartini ◽  
Try Zulchi

<p><strong>Abstract</strong></p><p>The research was aimed to screen the local rice germplasms for salinity tolerance. The 104 local rice germplasms were derived of collection of Gene Bank of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (ICABIOGRAD) were tested to salinity. The activities was carried out in greenhouse of ICABIOGRAD. The 15 day old seedlings were planted in pots soil contained NaCl 0.4%. One accession of rice was planted in 2 pots, each pot contained 4 seedlings. Observation of plant symptom was conducted after 1 month planted. The experiment was carried out in a Completely Randomized Design with 2 replications. The results of the analysis showed there were different responsses among rice accesions to the treatment. Salinity caused dry and dead leaves, there were very significant differences between the varieties for plant height, dry weight plant, number of total leaves, and number of green leaves. There were a high and negative correlation of plant height and total number of leaves with percentage of dead leaves. The result showed 21 accesions were tolerant to moderately&amp;#8722;tolerant, while Pokkali as a tolerant check, most tolerant. There were two accessions were tolerant with dead leaf percentage &lt;50%, i.e. Tjempo Brondol (reg. 5800) and Gembira Putih (reg. 20602), whereas Pokkali as resistant check had low dead leaf percentage (16.9%) and 19 accessions were moderately-tolerant with dead leaf percentage &lt;70%, and 82 accesions were sensitive to highly-sensitive.</p><p><strong>Abstrak</strong></p><p>Penelitian ini bertujuan untuk menyaring plasma nutfah padi yang memiliki sifat toleran terhadap salinitas. Sejumlah 104 plasma nutfah padi lokal yang berasal dari koleksi Bank Gen BB Biogen diuji terhadap salinitas. Kegiatan dilakukan di rumah kaca BB Biogen. Bibit padi berumur &amp;plusmn;15 hari ditanam pada pot berisi tanah yang sudah dilumpurkan dengan larutan garam NaCl 0,4% (4.000 ppm). Satu aksesi padi ditanam pada 2 pot, masing masing pot berisi 4 tanaman. Setelah berumur 4 minggu dari tanam dilakukan pengamatan meliputi tinggi tanaman, bobot kering akar, bobot kering tanaman, jumlah daun total, jumlah daun hijau, dan jumlah daun mati. Pengujian menggunakan Rancangan Acak Lengkap dengan 2 ulangan. Hasil analisis varians menunjukkan terdapat perbedaan respons plasma nutfah padi terhadap perlakuan salin yang diberikan. Perbedaan sangat nyata terdapat pada tinggi tanaman, bobot kering tanaman, jumlah daun total, dan jumlah daun hijau. Terdapat korelasi yang tinggi dan negatif pada tinggi tanaman dan jumlah daun total dengan persentase daun mati. Hasil pengujian telah terpilih 21 aksesi plasma nutfah padi toleran hingga agak toleran, sedangkan Pokkali sebagai cek sangat toleran. Dua aksesi yang toleran dengan persentase daun mati &lt;50%, yaitu Tjempo Brondol (reg. 5800) dan Gembira Putih (reg. 20602), Pokkali sebagai varietas kontrol dengan tingkat persentase daun mati 16,9%, sedangkan 19 aksesi termasuk kelompok agak toleran dengan persentase daun mati &lt;70%, dan 82 aksesi termasuk kelompok peka hingga sangat peka.</p>


2019 ◽  
Vol 18 (2) ◽  
pp. 169
Author(s):  
Selvi Helina ◽  
Sri Sulandari ◽  
Sedyo Hartono ◽  
Andi Trisyono

Detection and Transmission of  rice stunt virus on Ciherang and Situ Bagendit Varieties. The explosion of brown planthoppers recently has caused reduction of rice production in Indonesia. Brown planthoppers do not only act as pest, but also transmit Rice grassy stunt virus (RGSV) and Rice ragged stunt virus (RRSV). Detection of the existence of the two viruses in rice plants and vector insects is important to be done to ensure that the virus is infected with the vector. The aim of this research is to detect the existence of virus in varieties of Ciherang and Situ Bagendit as a result of transmission in the laboratory and to find out the ability of brown planthoppers to transmit stunt virus to both of the varieties. This research was compiled using Completely Randomized Design (CRD) with 4 treatments, namely healthy rice plants of Ciherang and Situ Bagendit varieties, Ciherang and Situ Bagendit varieties which were infested by brown planthoppers each with 5 repetitions. The parameters observed were incubation period, symptoms, plant height, number of leaves and incidence of disease. The data on plant height, number of leaves and incidence of disease were analyzed using ANOVA and continued with the Least Significant Difference (LSD) test at the level of 5%. The results showed that Ciherang and Situ Bagendit varieties were only positively infected by Rice ragged stunt virus. The results of the rice transmission showed that Ciherang variety had a faster incubation period of 10 DAI while Situ Bagendit was 14 DAI, but the two varieties showed an inhibition of growth in plant height and number of leaves compared to healthy plants with each incidence of 51.3% and 46.3%.


Akta Agrosia ◽  
2020 ◽  
Vol 23 (2) ◽  
pp. 67-71
Author(s):  
Fiana Podesta ◽  
Ririn Harini ◽  
Dwi Fitriani ◽  
Suryadi Suryadi

Soybean production in Bengkulu Province is still lower than national production. Soybean production can still be improved by adding nutrients, one source of untapped nutrients is cow's blood which is still a waste. The purpose of this research is to determine the effect of cow’s blood added with bioactivators on growth performance of soybean. This research was conducted by using a complete randomized design (CRD) arranged factorially consisted of two factors with three replications. The first factor was the various kinds of cow’s blood added with bioactivators consisting of Yeast, Stale Rice, Microorganism-Bio(M-Bio), Effective Microorganism-4 (EM-4), and Rumen. The second factor was the concentration of cow’s blood added with bioactivators consisting of 15%, 30%, and 45%. Results showed: 1) There was no treatments interaction between various kinds of cow’s blood added with bioactivators and its concentration in the growth, 2) the various kinds of cow’s blood added with  bioactivators had significant effect in the growth of plant height, number of leaves, number of branches, wet and dry weight, but it had no significant effect on plant height and leaf area, and 3) The concentration of cow’s blood added with bioactivators  has no significant effect in the growth.  This study indicated that cow’s blood added with Rumen as bioactivator showed better growth performance of soybean. Keywords: bioactivators, cow’s blood, nutrient of land, performance of soybean


2019 ◽  
Vol 6 (3) ◽  
pp. 477-481
Author(s):  
Dini Puspita Yanty ◽  
Siti Hardianti Wahyuni

Effect of immersion duration of trichoderma harzianum on local chili growth the aim of this researchis to see the old soaking ability of Trichoderma harzianum mushroom on the growth of local chili.The design used was a Completely Randomized Design (CRD) consisting of 6 treatments and 4replications, namely local chili seeds soaked with a 5-minute T.harzianum suspension, local chiliseeds soaked with a 10-minute T.harzianum suspension, local chili seeds that were soaked marinatedwith T.harzianum suspension for 15 minutes, local chili seeds soaked with T.harzianum suspensionfor 20 minutes, local chili seeds soaked with T.harzianum suspension for 25 minutes, Control (withoutimmersion with trichoderma harzianum suspension. Observed parameters are the percentage of local chiligrowth, plant height and number of leaves The results showed that the application of trichoderma harzianumhad a good effect on the growth of chili plants, soaking for 25 minutes was the best soaking on localchili seeds.


2018 ◽  
Vol 24 (3) ◽  
pp. 248-254 ◽  
Author(s):  
Ariadna Linares Gabriel ◽  
Catalino Jorge López-Collado ◽  
Mario Alejandro Hernández-Chontal ◽  
Joel Velasco-Velasco ◽  
Gustavo López-Romero

The Heliconias have a great potential as a cut flower and for this reason an adequate management of the crop is important. The objective of this research was the application of soil amendments (Biol and superabsorbent polymers - SAP) and the analysis on their effect in the growth of Heliconia psittacorum cv. Tropica. An experiment was established using a completely randomized design with four treatments and five repetitions: SAP (1 g plant-1), biol (20 mL plant-1) + SAP (1 g plant-1), biol (20 mL plant-1) and one control (without application). A 20 cm rhizome was planted per pot and were evaluated the height of the plant, the number of leaves, the leaf area and the number of shoots were measured. The analysis of variance did not show statistical differences (p ≤ 0.05) between treatments for the variables of the height of the plant, the leaf area and the number of shoots, but statistical differences were found for the variable number of leaves (p ≤ 0.05), and the best treatments were Biol, SAP, and Biol + SAP. With the application of the biol and SAP amendments, it was not possible to increase the plant height, the leaf area and the number of Heliconia shoots. Regarding the application of SAP to the not found significant results in Heliconia, it is expected that treatments with SAP show a better effect in the dry season.


2017 ◽  
Vol 5 (2) ◽  
pp. 43
Author(s):  
Muhammad Andry Kesuma ◽  
Indriyanto Indriyanto ◽  
Yusnita Yusnita

 Dendrobium (Orchidaceae) is one of the most popular orchid genera consisting more than 2000 species, which is widely known for its long vaselife, various, shapes and colors, high frequency of flowering per year and long and flexible stalks, so it is easily assembled for flower arrangements. The objectives of this research was to study the effects of different host trees and position of plant attachment at the tree on growth of Dendrobium seedlings. This experiment was conducted with treatments arranged factorally (3 X 2) in a completely randomized design with three replicates. The first factor was the species of host three consisted of Akasia (Acacia auriculiformis), Cemara Bundel (Cupressus retusa) and Kerai Payung (Filicium decipiens) and the second factor was position of plant attachment on the tree, which consisted of attachment at the bottom ( 0,5 m above ground ) and the upper was ( 1,5 m above ground ). Each experimental unit consisted of two Dendrobium seedlings. The results showed that in general both host trees and position of attachment did not affect number of shoots, number of leaves, plant height and the longest root length. however, different host trees and its interaction with the position of attachment affected the number of roots, resulted in the highest number of roots obtained in Kerai Payung (Fillicium decipiens) at 1,5 m above ground.Keywords: Acacia auriculiformis, Cupressus retusa, host tree, Filicium decipiens, orchid.


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