scholarly journals The radio-protective effects of n-Hexane extracts of Telfairia occidentalis Hook. f. and Cucumeropsis mannii Naud. seed oils on the liver of irradiated male Wistar rats

2020 ◽  
Vol 7 (3) ◽  
pp. 432-440
Author(s):  
Sarah Onyenibe NWOZO ◽  
Michael O UKEJE ◽  
Josiah Eseoghene IFIE
Keyword(s):  
Oxidative Stress ◽  
Ionizing Radiation ◽  
Histological Examination ◽  
Wistar Rats ◽  
Male Rats ◽  
Herbal Remedies ◽  
Protective Effects ◽  
Telfairia Occidentalis ◽  
Hexane Extracts ◽  
Cucumeropsis Mannii

The human population is predisposed to some considerable amounts of radiation especially ionizing radiation which may negatively impact their metabolic processes. Herbal extracts can mitigate these harmful effects. Therefore, this study aims to investigate the protective effect of n-Hexane extracts of Telfairia occidentalis and Cucumeropsis mannii seeds oils against radiation-mediated oxidative stress in Wistar rats. Sixty male rats were randomly distributed into six groups of six animals each and n-hexane extracts of T. occidentalis and C. mannii were administered at a dose of 2.4 or 4.8 mg/kg b. wt., orally for 7 days before irradiation and 10 days after irradiation, when they were sacrificed. Lipid peroxidation was measured, hepatic antioxidant status; SOD, CAT, GSH, Gpx and GST were estimated. The activities of liver enzymes: ALT, AST and ALP were measured and histological examination of sections of the liver was carried out. Radiation significantly increased MDA levels, SOD, GPx, AST, ALT and ALP activities but reduced body weights, total proteins, CAT, GSH and GST activities. Administration of the extracts significantly reduces the levels of MDA, SOD, GPx, ALT, AST and ALP activities while they increase the activities of CAT, GSH and GST at a dosage of 4.8 mg/kg. Histological examination showed increased levels of toxicity in radiated and groups administered 2.4 mg/kg extracts. From these findings, extracts of T. occidentalis and C. mannii at 4.8 mg/kg b. wt are effective herbal remedies in the prevention and amelioration of the consequences of oxidative stress due to exposure to ionizing radiation.

The Protective Effect of Metformin against Oxandrolone-Induced Infertility in Male Rats

2020 ◽  
Vol 26 ◽  
Author(s):  
Abdulqader Fadhil Abed ◽  
Yazun Bashir Jarrar ◽  
Hamzeh J Al-Ameer ◽  
Wajdy Al-Awaida ◽  
Su-Jun Lee
Keyword(s):  
Gene Expression ◽  
Male Infertility ◽  
Protective Effect ◽  
Histological Examination ◽  
Sperm Count ◽  
Serum Testosterone ◽  
Male Rats ◽  
P Value ◽  
Protective Effects ◽  
Rt Pcr

Background: Oxandrolone is a synthetic testosterone analogue that is widely used among bodybuilders and athletes. However, oxandrolone causes male infertility. Recently, it was found that metformin reduces the risk of infertility associated with diabetes mellitus. Aim: This study aimed to investigate the protective effects of metformin against oxandrolone-induced infertility in male rats. Methods: Rats continuously received one of four treatments (n=7) over 14 days: control DMSO administration, oxandrolone administration, metformin administration, or co-administration of oxandrolone and metformin. Doses were equivalent to those used for human treatment. Subsequently, testicular and blood samples were collected for morphological, biochemical, and histological examination. In addition, gene expression of the testosterone synthesizing enzyme CYP11A1 was analyzed in the testes using RT-PCR. Results: Oxandrolone administration induced male infertility by significantly reducing relative weights of testes by 48%, sperm count by 82%, and serum testosterone levels by 96% (ANOVA, P value < 0.05). In addition, histological examination determined that oxandrolone caused spermatogenic arrest which was associated with 2-fold downregulation of testicular CYP11A1 gene expression. However, co-administration of metformin with oxandrolone significantly ameliorated toxicological alterations induced by oxandrolone exposure (ANOVA, P value < 0.05). Conclusion: Metformin administration protected against oxandrolone-induced infertility in male rats. Further clinical studies are needed to confirm the protective effect of metformin against oxandrolone-induced infertility among athletes.

Indicators of oxidative stress in weanling and pubertal rats following exposure to nicotine via milk

2009 ◽  
Vol 29 (6) ◽  
pp. 489-496 ◽  
Author(s):  
Ben Ahmed Halima ◽  
Khlifi Sarra ◽  
Rtibi Kais ◽  
Elfazaa Salwa ◽  
Gharbi Najoua
Keyword(s):  
Oxidative Stress ◽  
Wistar Rats ◽  
Female Rats ◽  
Male Rats ◽  
Nicotine Exposure ◽  
Lactation Period ◽  
Aspartate Amino Transferase ◽  
Males And Females ◽  
Breast Fed ◽  
Biomarkers Of Oxidative Stress

Nicotine, a major toxic component of tobacco, has been identified as an important risk factor for infant and children diseases. It is concentrated in breast milk and is absorbed by the infant. The purpose of the present study was to investigate the effects of maternal nicotine exposure during lactation on breast-fed rats and at the pubertal age by measuring biomarkers of oxidative stress. Particularly, a new parameter, the thiol concentration was evaluated. Two groups of lactating Wistar rats were used. For the first group, female rats were given an intraperitoenal injection of nicotine or saline (2 mg/kg per day) during lactation. For the second group, we reproduced the same process described above and then the female and male pups were separately kept after weaning without any treatment until the puberty (at 45 days of age). In the liver and lung of the offspring, we examined the malondialdehyde (MDA) level, the thiol concentration, and the activities of two antioxidant enzymes: superoxyde dismutase (SOD) and catalase (CAT). In the plasma, alanine amino transferase (ALT) and aspartate amino transferase (AST) activities were measured. For rats aged 21 days, the treatment significantly reduced the thiol concentration, SOD, and CAT activities but increased MDA level, AST, and ALT activities. For rats aged 45 days, the males and females did not react the same way. In fact, the males were more affected. These results indicate that maternal nicotine exposure during the lactation period induces oxidative stress in the liver and lung of lactating offspring, which is maintained until the puberty, especially for the male rats.

scholarly journals Protective Effects of Parkia biglobosa Protein Isolate on Streptozotocin-Induced Hepatic Damage and Oxidative Stress in Diabetic Male Rats

Molecules ◽  
2017 ◽  
Vol 22 (10) ◽  
pp. 1654 ◽  
Author(s):  
Bolajoko Ogunyinka ◽  
Babatunji Oyinloye ◽  
Foluso Osunsanmi ◽  
Andrew Opoku ◽  
Abidemi Kappo
Keyword(s):  
Oxidative Stress ◽  
Protein Isolate ◽  
Hepatic Damage ◽  
Male Rats ◽  
Protective Effects ◽  
Parkia Biglobosa ◽  
And Oxidative Stress

scholarly journals Anti-Inflammatory and Antioxidative Effects of Sumatriptan Against Doxorubicin-Induced Cardiotoxicity in Rat

2021 ◽  
Author(s):  
Mohammad Sheibani ◽  
Hedyeh Faghir-Ghanesefat ◽  
Yaser Azizi ◽  
Tahmineh Mokhtari ◽  
Hasan Yousefi‐Manesh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Mortality Rate ◽  
Cardiac Tissue ◽  
The Body ◽  
Male Rats ◽  
Protective Effects ◽  
Cardiac Damage ◽  
Necrosis Factor Alpha ◽  
Antioxidative Effects

The clinical use of doxorubicin as a potent chemotherapeutic agent is limited due to its dose-dependent cardiotoxicity. Oxidative stress and inflammatory pathways have a pivotal role in doxorubicin-induced cardiotoxicity. Sumatriptan, a 5-hydroxytryptamine (5-HT)1B/1D agonist that is mainly used to relieve migraine pain, has suggested exerting protective effects in numerous pathological conditions through antiinflammatory properties. The aim of the present study was to investigate the effects of sumatriptan on doxorubicin-induced cardiotoxicity and the contribution of anti-inflammation and antioxidative responses. Cardiotoxicity was induced by the administration of doxorubicin three times a week (2.5 mg/kg i.p) for two consecutive weeks on male rats. The animals were divided into four groups, including Control, Sumatriptan (0.1 mg/kg) received group, doxorubicin received group, and Doxorubicin+Sumatriptan (0.1 mg/kg) received group. Sumatriptan was administered 30 min before every injection of doxorubicin. On the last day of the second week, the body weight, mortality rate, electrocardiogram (ECG) and histopathological changes, cardiac inotropic study, and biochemical factors were evaluated. The loss of body weight, mortality rate, ECG parameters, reduction of papillary muscle contractility force as well as histopathological scores following administration of doxorubicin indicated severe cardiac damage. However, treatment with sumatriptan inhibited the functional and structural impairment induced by doxorubicin. In addition, sumatriptan could significantly reduce cardiac tissue levels of malondialdehyde (MDA) and tumor necrosis factor-alpha (TNF-α), which were increased in the doxorubicin-treated rats. This study illustrated the protective effects of sumatriptan on decreasing doxorubicin-induced cardiac toxicity and mortality rate in part through inhibition of inflammatory and oxidative stress pathways.

scholarly journals Subchronic Toxicological Assessment of Dr Iguedo Goko Cleanser® on Lipid Profile and Serum Antioxidant Enzymes in Exposed Wistar Rats

2020 ◽  
pp. 16-25
Author(s):  
Godswill J. Udom ◽  
Jude E. Okokon ◽  
John A. Udobang ◽  
Daniel N. Obot ◽  
Nkechi J. Onyeukwu
Keyword(s):  
Oxidative Stress ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Low Dose ◽  
Herbal Remedy ◽  
Female Rats ◽  
Male Rats ◽  
High Density Lipoproteins ◽  
High Dose ◽  
Low Density

Dr Iguedo Goko Cleanser® is a polyherbal mixture promoted as an effective herbal remedy for numerous diseases. Study aimed to evaluate the toxicity concern of the polyherbal mixture (PHM) on lipid profile and oxidative status in Wistar rats of both gender. Acute toxicity study was conducted using modified method of Lorke. Thirty Wistar rats of bother gender were randomly divided into six groups (5/group) and exposed to the polyherbal mixture for 60 days via oral gavage. Control groups (1 and 4) received 10 mL/kg distilled water, while groups 2-3 and 5-6 received 476.24 and 158.75 mg/kg body weight of Dr Iguedo Goko Cleanser® respectively. On 62nd day, animals were sacrificed under diethyl ether anaesthesia; blood samples were collected by cardiac puncture for biochemical analysis. PHM significantly (p < 0.05) increased high density lipoproteins (HDL) levels in male rats as well as high dose female rats relative to control. However, low dose female rats recorded low HDL levels relative to control. Total cholesterol, triglycerides, low density and very low density lipoprotein levels were significantly reduced in all test groups relative to controls. The low dose males (LDM) had reduced serum glutathione peroxidase (GPX) activity; while increased and decreased GPX and glutathione (GSH) activities were respectively recorded for female rats. Male rats had dose-dependent increase in malondialdehyde. The recorded reductions in serum lipids suggest that the polyherbal mixture may have hypolipidemic potentials. While the increased malondialdehyde as well as decreased GPX and GSH indicate lipid peroxidation and oxidative stress inducing potentials of the PHM. Despite the positive modulation on lipid profile, findings suggest utmost caution on chronic use as its oxidative stress inducing potentials is considerable.

Effect of barley grain on memory and brain’s oxidative stress factors in male rats

2020 ◽  
Vol 10 ◽  
Author(s):  
Batool Shakiba ◽  
Azam Moghani ◽  
Marzieh Kafami ◽  
Mahmoud Hosseini ◽  
Masoud Hosseinzadeh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Animal Feed ◽  
Memory Function ◽  
Barley Grain ◽  
Stress Factors ◽  
Male Rats ◽  
Barley Seed ◽  
Saline Control ◽  
Albino Rats ◽  
Protective Effects

Background & Objective: Barley is widely used as a major staple of human food and animal feed. Several antioxidant phenols are found in barely, which have scavenging properties. The present study aimed to assess the protective effects of barley seed against the oxidative damage of brain tissues in a scopolamine-induced memory impairment model. Materials and Methods: In total, 32 male albino rats (mean weight: 250±10 g) were divided into four groups of saline (control), scopolamine, barley seed (100 mg/kg) with scopolamine, and barley seed alone. The spatial memory function was assessed using the Morris water maze. Results: Compared to the scopolamine group, barley seed could decrease the escape latency time in the treated rats, while the time spent and distance traveled in the target quadrant on the probe trial increased. Moreover, barley seed could increase the malondialdehyde concentration in the hippocampus and cortical tissues, while the thiol content was observed to decrease. Conclusion: According to the results, the use of dietary barley seed could improve the memory function in dementia associated with increased oxidative stress.

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