scholarly journals Features of the immunoreactivity T and B lymphocytes subpopulations and cytokine imbalance in patients with hepatosplenomegaly of different etiology

Pathologia ◽  
2021 ◽  
Vol 18 (2) ◽  
pp. 174-182
Author(s):  
O. M. Klimova ◽  
T. I. Kordon ◽  
S. V. Sushkov ◽  
L. A. Drozdova ◽  
O. V. Lavinska ◽  
...  
Keyword(s):  
B Lymphocytes ◽  
B Lymphocyte ◽  
Group Iii ◽  
Regulatory T Lymphocytes ◽  
Normal Number ◽  
Group I ◽  
Hla Dr ◽  
Vascular Growth Factor ◽  
Maximum Decrease ◽  
High Level

The aim was to study the mechanisms of immunological dysregulation of cytokine and immunoglobulin production, changes in the CD expression of T and B lymphocyte subpopulations in patients with hepatosplenomegaly of different etiology. Materials and methods. We examined 73 patients with liver cirrhosis complicated by portal hypertension, hepatosplenomegaly, and bleeding from phlebectasia. We identified three groups of patients depending on the triggering factors of cirrhosis: the first (I) group – HBV/HCV; the second (II) group – CMV/VEB; the third (III) group – hereditary enzymopathies. The study material was lymphocytes and blood serum. We used the methods of ELISA, immunofluorescence and flow cytometry. Results. An increase in the concentration of IgA and IgM was revealed against the background of normal number of CD22+ B lymphocytes with HBV/HCV (I group), high level of IgM and their producers, B lymphocytes, with CMV/VEB (II group), in group III with hereditary enzymopathies, the concentration of all immunoglobulins was normal with an increased content of B lymphocytes. Multidirectional changes in the content of cytokines were revealed: in group I the synthesis of anti-inflammatory cytokines IL-4, IL-10 and in group II – pro-inflammatory IL-1β, INF-γ, TNF-α dominated; in group III the concentration of IL-6 and vascular growth factor (VEGF) was maximally increased. The number of activated CD3+CD4+CD25+ T cells was reduced in groups I and II – by 2.3 and 2.0 times respectively, in group III – increased by 1.2 times. The number of regulatory T lymphocytes CD3+CD4+CD25+CD127neg was reduced by half in I and II groups. Expression of co-stimulatory molecules CD3+CD4+CD28+ was low in all groups and the maximum decrease was in group III. In patients with HCV/HBV, an increase in the expression of the late activation marker of lymphocytes CD3+HLA-DR+ by an average of 63 % was noted. Conclusions. The revealed immune disorders in hepatosplenomegaly of different etiology are characterized by multidirectional changes. Approaches to the treatment of these patients should be complex, taking into account the trigger factors that cause dysregulation of immune responses, which leads to further destruction, and focuses at remodeling target organs.

scholarly journals Variability of Phytochemicals by Breeding Year, Usage and Seed Size of Korean Soybean (Glycine max (L.) Merrill) Varieties

Agriculture ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 100
Author(s):  
Tae-Young Hwang ◽  
Byoung-Deok Park ◽  
Jwakyung Sung ◽  
Hong-Sig Kim
Keyword(s):  
Cluster Analysis ◽  
Seed Weight ◽  
Group Iv ◽  
Group Iii ◽  
Group I ◽  
Glycine Max L ◽  
Main Components ◽  
High Level ◽  
Different Origins ◽  
100 Seed Weight

The interest in phytochemicals from crops has grown in recent years, with soybean, as a functional crop, having great potential for providing a high level of nutrition. Therefore, the aim of this study was to evaluate phytochemicals from Korean soybean varieties, and to provide the basic information necessary for targeted breeding of soybean. The seeds of 172 soybean varieties were collected from various domestic institutes, and their phytochemicals were analyzed and compared based on agronomic characteristics (color, size, usage, etc.) using cluster analysis. We found that the soybean varieties contained relatively higher levels of the following phytochemicals: lutein (3 var., >7.0 μg g−1 seed), tocopherols (26 var., >300 μg g−1 seed), and phytosterols (33 var., >1000 μg g−1 seed). Overall, phytochemical levels were higher in varieties released since 2000 and with small-sized seeds (<12 g, 100-seed weight). The distribution of phytochemicals varied according to the usage (cooking, sauce, sprouts, etc.), even though the highest levels of each phytochemical were mainly observed in sprouts. The cluster analysis using three main components classified the varieties into five groups. Of them, group III showed relatively higher tocopherols, group IV had relatively higher lutein, and group I had relatively higher phytosterols. Jonam and Manpoong (group I) were observed in varieties high in phytochemicals. Overall, some Korean soybean varieties were found to have relatively higher levels of phytochemicals compared to those reported in previous studies of other origins. In conclusion, our findings suggest that some varieties might provide suitable breeding material for target-centered improvement of soybean varieties, and a comparison between different origins is necessary for providing better knowledge for soybean breeding.

HEIFERS POSTSLAUGHTER MEAT PRODUCTIVITY EVALUATION AT DIET USE OF FEED ADDITIVES BIODARIN

2016 ◽  
Vol 1 (1) ◽  
pp. 30-32
Author(s):  
Тагиров ◽  
Khamit Tagirov ◽  
Долженкова ◽  
Galina Dolzhenkova
Keyword(s):  
Meat Quality ◽  
Meat Products ◽  
Feed Additives ◽  
Feed Additive ◽  
Control Group ◽  
Chemical Compositions ◽  
Group Iii ◽  
Group I ◽  
Meat Productivity ◽  
High Level

The purpose of research focused on post-slaughter evaluation of meat productivity and meat quality the Kazakh White-headed breed when introduced into the diet of feed additives BioDarin. Presents the results of the control slaughter heifers 18 months old as well as the results of studies of morphological and chemical compositions of the carcass. The research was conducted post-slaughter evaluation of meat productivity and meat quality of heifers of Kazakh White-headed breed when introduced into the diet of feed additives BioDarin. Scientific and economic experiment was carried out on the basis of JSC AIC «Alga+» Tuimazinsk district of the Republic of Bashkortostan. For the experiment there were formed 4 groups of heifers of Kazakh white-headed breed: group I – control; group II heifers in addition to the basic diet received a feed additive in a dose of 0.5 kg per 100 kg of grain mixture, the animals of group III received 1 kg of the feed additive per 100 kg of grain mixture, peers IV groups received 1.5 kg of a grain mixture. Among the studied groups, heifers of the III experienced group receiving feed additive at a dose of 1.0 kg per 100 kg of grain mixture, had a high level of meat productivity. It was found that meat products of the best quality was got also from animals of group III.

scholarly journals Problem of Ametropia in Children in Russian Federation

2018 ◽  
Vol 15 (2S) ◽  
pp. 40-44
Author(s):  
El Yu. Markova ◽  
L. Yu. Bezmel’nitsyna ◽  
O. V. Kurganova ◽  
N. A. Pron’ko ◽  
L. V. Venediktova
Keyword(s):  
Electrophysiological Study ◽  
Economic Assessment ◽  
Medical Costs ◽  
Refractive Errors ◽  
Mathematical Methods ◽  
Group Iii ◽  
Social Burden ◽  
Group I ◽  
High Level

Refractive errors are remaining one of the most important problems in pediatric ophthalmology. At present, there are changes in organization approaches of prophylactic medical examinations in children. It causes the necessary of comprehensive clinical and economic assessment of their effectiveness.Purpose: to develop the recommendations for improvement of ophthalmological care for children with refractive errors. For diagnostics we used visometry, determination the vision characteristics, determination of chromosensitivity, biomicroscopy, definition of refraction of manifest and cycloplegic, determination of volume and accommodation reserves, ophthalmoscopy, electrophysiological study (EFI), optical coherence tomography (OCT), fundusography.Methods. A retrospective analysis of typical practice of treatment patients with amblyopia and strabismus was organized. Data was processed with mathematical methods and descriptive statistics. Modeling, Budget impact and burden of diseases analyses were used for clinicoeconomical evaluation.Results. 636 patients aged from 5 to 7 years old were included. Depending on the age of the diagnosis of ametropia, three groups were identified. Group I (318 patients): the diagnosis was detected up to 1 year, group II (190 patients) — at the age from 1 to 3 years, Group III: (127 patients) from 3 to 7 years. A direct correlation between the age of ametropia diagnostics and frequency such complications as amblyopia, disturbance of binocular vision and strabismus development of strabismus was established. According to the results of clinical and economic analysis, uncorrected refractive errors in children are characterized by a high level of economic and social burden of disease (the older the age of ametropia diagnosis caused increase of direct medical and non-medical costs). With regard to non-medical costs, we established that a reduction the frequency of courses in vision office and operational treatment results in a reduction in payments to parents on temporary incapacity sheets.Conclusion: the optimal age for diagnostics of refractive errors in up to 1year from both clinical and economic perspective. 

scholarly journals PRO-ENVIRONMENTAL ACTIVITIES IN POLAND IN 2015 (REGIONAL STUDY)

2017 ◽  
Vol 16 (4) ◽  
pp. 93-101 ◽  
Author(s):  
Karol Kukuła
Keyword(s):  
Complex Phenomenon ◽  
Regional Study ◽  
Diagnostic Features ◽  
Group Iii ◽  
Group I ◽  
Construction Techniques ◽  
The Status ◽  
Multiple Criteria Evaluation ◽  
High Level ◽  
Environmental Activities

The author of the article sets out to perform two tasks. The first is an attempt to present the construction techniques of a ranking of items (in this case – voivodships) in terms of the level of a complex phenomenon, and then to present a method of division of items into groups with similar values of the synthetic variable. The second goal is to present the condition of pro-environmental activities in Poland in 2015 in the regional system. In order to implement this objective, diagnostic variables have been selected, describing the condition of activities for environmental protection. Based on the following criteria: substantive and sufficient variability, 11 diagnostic features have been selected. These variables constitute the basis for multiple criteria evaluation of a complex phenomenon (pro-environmental activities). Based on the described procedures, a ranking of voivodeships has been created on the basis of the status of pro-environmental activities in Poland. The set of items (voivodships) has been divided into 3 groups: group I – voivodships with a high level of pro-environmental activities, group II – voivodships with an average level, and group III with a relatively low level. The quantitative proportions of voivodships in particular groups are as follows, respectively: 4 : 6 : 6.

Decreased HLA (human leucocyte antigen)-DR expression on peripheral blood monocytes predicts the development of organ failure in patients with acute pancreatitis

2003 ◽  
Vol 105 (4) ◽  
pp. 409-417 ◽  
Author(s):  
Panu MENTULA ◽  
Marja-Leena KYLÄNPÄÄ-BÄCK ◽  
Esko KEMPPAINEN ◽  
Annika TAKALA ◽  
Sten-Erik JANSSON ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Organ Dysfunction ◽  
Immune Suppression ◽  
Human Leucocyte Antigen ◽  
Severe Disease ◽  
Positive Likelihood Ratio ◽  
Group Iii ◽  
Group I ◽  
Hla Dr ◽  
Cellular Marker

Immune suppression plays an important role in the pathogenesis of acute pancreatitis. Monocyte expression of HLA (human leucocyte antigen)-DR, a cellular marker of immune suppression, was determined in relation to the development of organ dysfunction in patients with acute pancreatitis. A total of 310 consecutive patients with acute pancreatitis, admitted to a university hospital within 72 h of pain onset, were studied; 194 (63%) had mild disease (group I), 87 (28%) had severe disease without organ dysfunction (group II), and 29 (9%) had severe disease with organ dysfunction (group III). HLA-DR expression, defined both as the proportion of monocytes that were HLA-DR-positive and as monocyte HLA-DR fluorescence intensity, was determined at admission, using whole-blood flow cytometry. Of the patients in group III, 13 (45%) developed organ dysfunction within 24 h of admission. The proportion of HLA-DR-positive monocytes and monocyte HLA-DR density were both related to the severity of pancreatitis (P<0.001 for linear trend). In predicting organ dysfunction, the sensitivity, specificity and positive-likelihood ratio for the proportion of HLA-DR-positive monocytes were 83% [95% CI (confidence interval) 64–94%], 72% (67–77%) and 3.0 respectively, and for monocyte HLA-DR density the respective values were 69% (49–85%), 84% (79–88%) and 4.3. In conclusion, monocyte HLA-DR expression predicts the development of organ dysfunction that occurs early in patients with acute pancreatitis.

scholarly journals Impact of livestock industries on rural development

2021 ◽  
Vol 22 (4) ◽  
pp. 608-619
Author(s):  
Alexander I. Kostyaev ◽  
Galina N. Nikonova
Keyword(s):  
Rural Development ◽  
Rural Areas ◽  
Cattle Breeding ◽  
Group Iii ◽  
Livestock Breeding ◽  
Group I ◽  
The Russian Federation ◽  
High Level ◽  
Poultry Farming

The purpose of the study is to test the hypothesis of the priority role of livestock breeding in the development of rural areas on the basis of an analysis of livestock industries and determine the degree of territorial heterogeneity of this phenomenon. The study was carried out on the example of 29 regions of the Non-Black Earth Zone of the Russian Federation. Methods of ranking, correlation and variational analysis were used. A noticeable correlation was established between the indicators of the development of the livestock industry and rural development (p = 0.5; 0.6). For poultry farming, this relationship is moderate (p = 0.3; 0.3), pig breeding - weak negative (p = - 0.1; - 0.1). Concrete calculations confirmed the lack of influence of the development of pig breeding on one of the important indicators of the development of rural areas - the growth of acreage. The features of territorial heterogeneity in the development of cattle breeding and rural areas have been consistently investigated on the basis of the same methodology. Three equal groups of regions with a relatively high, medium and relatively low level of their development were identified. It is shown that the group of regions with a relatively high level of development of cattle breeding in the Non-Black Earth Region accounts for about half of the volume of milk and 56% of the production of cattle for slaughter. Neighboring regions in group I form two, in group II - one, in group III - three areas. When comparing the results obtained for cattle breeding and rural areas, it was found that the composition of the regions in the corresponding groups coincides by 67-78 %. When calculating the correlation coefficient between the final ranks of the regions, established when determining the heterogeneity of the development of livestock breeding and the heterogeneity of the development of rural areas, a high tightness of the correlation was revealed (p = 0.7). The hypothesis being tested was confirmed.

Rural–Urban Comparison in Prevalence of Overweight and Obesity Among Children and Adolescents of Asian Indian Origin

2011 ◽  
Vol 23 (6) ◽  
pp. 928-935 ◽  
Author(s):  
Arnab Ghosh
Keyword(s):  
Children And Adolescents ◽  
Parental Education ◽  
Age Groups ◽  
Overweight And Obesity ◽  
Healthy Children ◽  
Group Iii ◽  
Global Epidemic ◽  
Group I ◽  
High Level ◽  
Obesity In Children

Objectives. To study whether the prevalence of overweight and obesity in children and adolescents is restricted by habitat (urban vs rural). Methods. A total of 753 healthy children and adolescents, out of which 293 (159 boys and 134 girls) were collected Santiniketan (rural area) and 460 (241 boys and 219 girls) were from Calcutta and the suburbs (urban area), aged 8 to 18 years took part in the study. Participants were subsequently divided into 3 age groups: group I, 8 to 12 years; group II, 13 to 15 years, and group III, 16 to 18 years. Results. It was observed that the overall prevalence of overweight and obesity in the study was 9.6% and 5.7%, respectively. Urban residence and high level of parental education are associated with overweight and obesity in children and adolescents ( R2 = 40.3%). Conclusion. Prevalence of overweight and obesity among children and adolescents is not restricted to any particular habitat and warrants early intervention if this global epidemic is to be checked.

scholarly journals Immunogenicity and safety of subunit influenza vaccines in pregnant women

2018 ◽  
Vol 4 (2) ◽  
pp. 00060-2017 ◽  
Author(s):  
Mikhail P. Kostinov ◽  
Alexander P. Cherdantsev ◽  
Nelli K. Akhmatova ◽  
Daria A. Praulova ◽  
Aristitsa M. Kostinova ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Pregnant Women ◽  
Influenza Vaccines ◽  
Group Iii ◽  
Group I ◽  
Increased Risk ◽  
Protective Antibodies ◽  
Group Ii ◽  
High Level ◽  
Virus Strains

Pregnancy is a condition of modulated immune suppression, so this group of patients has increased risk of infectious diseases.Trivalent subunit vaccines, unadjusted Agrippal S1 (group I) and immunoadjuvant Grippol Plus (group II), containing 5 μg of actual influenza virus strains, were administered respectively to 37 and 42 women in the second and third trimester of physiological pregnancy.The administration of subunit influenza vaccines was accompanied by the development of local reactions in no more than 10% of patients, compared with 4.9% of the 41 pregnant women in the placebo group (group III). Systemic reactions were of a general somatic nature, did not differ between vaccinated and placebo groups, and were not associated with vaccination. Physiological births in groups I, II and III were 94.6%, 92.9% and 85.4%, respectively, and the birth rates of children without pathologies were 91.9%, 90.5% and 80.5%, respectively, and were comparable between groups. Vaccination stimulated the production of protective antibodies against influenza virus strains in 64.8–94.5% of patients after immunisation with an unadjusted vaccine and in 72.5–90.0% of patients after the administration of an immunoadjuvant vaccine. After 9 months, antibody levels were recorded in 51.3–72.9% in group I and 54.2–74.2% in group II. Immunisation against influenza in pregnant women provided a high level of seroprotection and seroconversion. Nevertheless, the level of seroprotection against the influenza strain A(H3N2, Victoria) was slightly lower in the group immunised with an unadjusted vaccine compared to those vaccinated with the immunoadjuvant vaccine.

Effects of Zinc Supplementation on the Immune System and on Antibody Response to Multivalent Influenza Vaccine in Hemodialysis Patients

1998 ◽  
Vol 21 (5) ◽  
pp. 274-278 ◽  
Author(s):  
S. TüRK ◽  
S. Bozfakioglu ◽  
S.T. Ecder ◽  
T. Kahraman ◽  
N. GüREL ◽  
...  
Keyword(s):  
Immune System ◽  
Antibody Response ◽  
Influenza Vaccine ◽  
Hemodialysis Patients ◽  
The Other ◽  
Group Iii ◽  
Group I ◽  
Hla Dr ◽  
Serum Zn ◽  
Antibody Levels

The depression of the immune system in chronic uremia is a well-known phenomenon but the role of serum zinc (Zn) levels on both cell-mediated and humoral immunity is still controversial. The aim of this study was to investigate the effect of Zn supplementation on the immune system and on antibody response to multivalent influenza vaccine (MIV) in hemodialysis patients (HP). Twenty-six HP and 11 healthy subjects (HS) were vaccinated with MIV. Hemodialysis patients were randomly divided into two groups. Group 1 (13 HP) was supplemented with 120 mg ZnS04 after each dialysis session. Group II (13 HP) and Grouip III (11 HS) were given placebo. In all cases, the serum Zn levels, CD3, CD4, CD8, CD19, HLA-DR+ cell percentages, CD4/CD8 ratio and CD3+HLA-DR+ cell percentages were determined before and 30 days after vaccination. Antibody levels to subgroups of MIV were also measured. All the baseline parameters studied were not statistically different between Group I and II. However, there was a significant difference between the basal parameters of Group III and the other two groups, except for CD3 and CD4 cell percentages. Serum Zn, CD19 cell percentage and antibody levels to MIV subgroups were significantly increased in Group I at the end of the first month of the study (p<0.01, p<0.05, p<0.001, p<0.001, and p<0.01, respectively), but the other parameters showed no significant changes. The only significant change observed in Groups II and III was an increase in antibody levels to MIV subgroups one month after vaccination. Antibody levels to MIV subgroups, were not statistically different between Groups I and II, but in Group III they were strikingly higher than those of HP (p<0.001). These results led us to conclude that Zn supplementation could not restore the immune parameters and enhance antibody response to MIV in HP.

scholarly journals Quality Assessment of Prosthetic Rehabilitation Using Aesthetic Fixed Restorations

2018 ◽  
Vol 25 (1) ◽  
Author(s):  
Zinovii Ozhohan ◽  
Andrii Biben
Keyword(s):  
Construction Materials ◽  
Clinical Effectiveness ◽  
Clinical Results ◽  
Occlusal Surface ◽  
Prosthetic Rehabilitation ◽  
Group Iii ◽  
Group I ◽  
Occlusal Surfaces ◽  
Group Ii ◽  
High Level

The objective of the research was to study and assess the quality of prosthetic treatment using aesthetic fixed restorations.Materials and methods. The study included 79 patients without a comorbidity who underwent prosthetic rehabilitation. All the patients were divided into 3 groups: Group I included 25 patients with metal-plastic restorations; Group II comprised 34 patients with porcelain-fused-to-metal restorations; Group III consisted of 20 patients with a combined occlusal surface of prosthetic restorations. The patients were observed 6 months after prosthetic repair. Only patients with single molar and premolar crowns were examined. Bridge prostheses were not taken into account in order to eliminate the effect of masticatory force redistribution on the abutment crowns.Results. In Group I, 11 (44%) patients were satisfied with the results of prosthetic treatment. In Group II, 25 (78.12%) patients reported that they were satisfied with their treatment. In Group III, there were 17 (85%) patients satisfied with their outcome. However, the patients’ complaints are often subjective and do not fully reflect the objective state of the dentoalveolar system. An objective examination revealed that in indirect restorations, marginal periodontium pathology is typical. Conclusions. Aesthetic fixed restorations with a combined occlusal surface have demonstrated good clinical results, even at long-term follow-up. Combining positive properties of two different construction materials, namely zirconium dioxide and ceramics, they reduce the risk of complications such as marginal periodontium pathology and chipping along the occlusal surface as well as contribute to minimal abrasion of the occlusal surfaces of the antagonistic teeth. We cannot recommend metal-plastic restorations due to their low clinical effectiveness, poor aesthetic qualities as well as a high level of marginal periodontium pathology.

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