scholarly journals A new combination of substrates: biogas production and diversity of the methanogenic microorganisms

2018 ◽  
Vol 13 (1) ◽  
pp. 119-128 ◽  
Author(s):  
Ivan Kushkevych ◽  
Monika Vítězová ◽  
Tomáš Vítěz ◽  
Jozef Kováč ◽  
Petra Kaucká ◽  
...  

AbstractAgriculture, food industry, and manufacturing are just some of the areas where anaerobic technology can be used. Currently, anaerobic technologies are mainly used for wastewater treatment, solid waste treatment, or for the production of electrical and thermal energy from energy crops processing. However, a clear trend is towards more intensive use of this technology in biomass and biodegradable waste processing and hydrogen or biomethane production. An enormous number of anaerobic digesters are operating worldwide but there is very little information about the effect of different substrate combinations on the methanogens community. This is due to the fact that each of the anaerobic digesters has its own unique microbial community. For the most effective management of anaerobic processes it would be important to know the composition of a consortium of anaerobic microorganisms present in anaerobic digesters processing different input combinations of raw material. This paper characterizes the effect of the input raw materials on the diversity of the methanogen community. Two predominant microorganisms in anaerobic digesters were found to be 99% identity by the sequences of the 16S rRNA gene to theMethanoculleusandThermogymnomonasgenera deposited in GenBank.

2011 ◽  
Vol 148-149 ◽  
pp. 688-691
Author(s):  
Jing Min Hong ◽  
Jing Zhou

A cost combined life cycle assessment was carried out to estimate the economic and environmental impact of biogas production from corn straw. Results showed that the pipeline transport, electricity, and chemicals production stages represented the dominant contribution to environmental impact in most mid-point categories, whereas infrastructure, waste treatment, pipeline transport, and raw materials (e.g., straw, chemicals, coal, diesel) price played an important role to economic impact. Results indicate that it is very difficult to develop the technology of straw for biogas production in China in case without a national financial support. In addition, optimizing the efficiency of raw material consumption and decreasing the pipeline transport distance are effective way to decrease the both overall environmental and economic burden.


2020 ◽  
pp. 0734242X2095740
Author(s):  
Lixian Wang ◽  
Yong Chi ◽  
Di Shu ◽  
Elsa Weiss-Hortala ◽  
Ange Nzihou ◽  
...  

Kitchen waste (KW) has gradually become a prominent problem in municipal solid waste treatment. Hydrothermal liquefaction (HTL) is a promising method used to make fuel oil from food and KW. However, the upgrading of bio-oil is particularly important for the sake of industrial reuse. In this study, the KW from university restaurants was subjected to HTL experiments in order to study theoretical feasibility. With the change of conversion temperature and residence time, the optimal conversion working conditions in this study were determined according to the quality and yield of the bio-oil. Moreover, the bio-oil upgrading effects of different additives (hydrogen chloride, sodium hydroxide, and iron(III) chloride) on the HTL of KW were studied. Alkaline additives have an inhibitory effect on the bio-oil yield and positive effect on coke yield. Acidic additives and iron (Fe)-containing additives can promote bio-oil yield. As an important aspect of upgrading, the effect on the nitrogen content of bio-oil with additives was revealed. The alkaline and Fe-containing additives have little effect on reducing the viscosity of the bio-oil while with the appropriate ratio (2.5 mol•kg−1) of acidic additives to the raw material, the static and dynamic fluidity of the oil phase products are reduced to about 0.1 Pa•s.


2021 ◽  
Vol 12 (2) ◽  
Author(s):  
Nazar Tkach ◽  
◽  
Tetiana Mirzoieva ◽  

The article presents a study on the justification of the economic feasibility of processing cereals into biogas. Аt the first stage the sown areas and productivity of separate agricultural crops in the investigated enterprises of Brovarsky district, Kyiv region were analyzed. The tendency to increase the yield of agricultural crops in the studied enterprises was revealed. This was seen as evidence that they could potentially be fully self-sufficient in raw materials for the production of gaseous biofuels. At the second stage of the study, the potential volume of the raw material base for biogas production in the studied enterprises was calculated, potential volume of biogas production and economically feasible volume of biogas production for each enterprise. It is proposed to use part of the grown grain for bioenergy production, and this part should be 10-15% of the gross harvest of grain enterprises in order to prevent the food crisis in the country. At the third stage of the study to determine the economic efficiency of biogas production from wheat and corn, investment costs were calculated, necessary for the implementation of the project to install a biogas plant in the studied enterprises. The authors calculated the investment costs required to implement the project of installing a biogas plant at the studied enterprises. The authors also analyzed the costs of each company for future projects. Finally, the economic efficiency of the proposed project for biogas production in the studied enterprises of Brovary district, Kyiv region is calculated, the economic efficiency of the project in the long run is analyzed with the use of discounted indicators of economic efficiency with a life cycle of 5 years. It was found that the implementation of projects for biogas production may be accompanied by high efficiency, all enterprises will receive profits from the implementation of investment projects for the production of biogas and biofertilizers with the subsequent sale of biogas and the use of biofertilizers for their own needs.


2014 ◽  
Vol 3 (2) ◽  
Author(s):  
Megawati Megawati

<p>Eceng gondok (Eichornia crassipes) merupakan tanaman yang menjadi limbah perairan dan keberadaannya belum banyak dimanfaatkan. Kandungan selulosa, hemiselulosa, dan lignin di dalamnya dapat dimanfaatkan menjadi biogas melalui proses fermentasi. Penelitian ini mengkaji pengaruh EM4 (Effective Microorganism- 4) terhadap massa, nilai kalor, dan kecepatan pembentukan biogas dari eceng gondok. Percobaan dilakukan dalam anaerobic digester berukuran 4 liter, bahan baku yang digunakan adalah eceng gondok, rumen sapi, dan air dengan variabel penambahan EM4 sebesar 1% dan 0%. Fermentasi dilakukan secara batch dengan pengukuran gas (temperatur, tekanan, dan massa) setiap 7 hari sekali sampai hari ke-35. Sebelum proses fermentasi, dilakukan pengujian terhadap rasio C/N campuran bahan baku. Pembakaran gas dilakukan untuk membuktikan gas yang didapat mengandung metana. Hasil Penelitian menunjukkan bahwa rasio C/N untuk variabel dengan penambahan EM4 1% sebesar 5,33 dan rasio C/N untuk variabel dengan penambahan EM4 0% sebesar 7. Jadi, penambahan EM4 dapat menurunkan rasio C/N. Sementara itu, hasil fermentasinya memperlihatkan bahwa EM4 memperkecil produksi biogas meskipun proses pembentukannya cepat. Massa total biogas yang didapat pada variabel EM4 1% sebesar 1,1 g dan variabel EM4 0% sebesar 1,55 g. Tekanan<br />biogas mengalami fluktuasi (pada variabel EM4 1% sebesar 35,6 cmH2O, sedangkan pada variabel EM4 0% sebesar 40,6 cmH2O). Berdasarkan simulasi menggunakan chemical process simulator software, diketahui heating value biogas sebesar 39.180 kJ/kg.</p><p> </p><p>Water hyacinth (Eichornia crassipes) is a plant that becomes waste and its existence has not been widely used. Content of cellulose, hemicellulose, and lignin in it can be converted into biogas through a process of fermentation. Study examines the effect of EM4 (Effective Microorganism-4) on the mass, heating value, and the rate of formation of biogas from water hyacinth. An experiments were performed in anaerobic digesters size of 4 liters, the raw material used is water hyacinth, cow's rumen, and water with variable of EM4 addition of 1% and 0%. Fermentation was carried out in batch condition with gas measurement (temperature, pressure, and mass) every 7 days until the 35th day. Before fermentation, the C/N ratio of raw material mixture was analyzed. The gas was burnt to prove that the obtained gas containing methane. Results showed that the C/N ratio for the variable with the addition EM4 1% is 5.33 and C/N ratio for the variable with the addition EM4 0% is 7. Thus, the addition of EM4 can reduce C/N ratio. Despite the formation process is rapid, the results showed that EM4 reduce the biogas production. Total mass of biogas obtained at variable<br />EM4 1% is 1.1 g and variable EM4 0% is 1,55 g. Biogas pressure is fluctuated (at variable EM4 1% is 35.6 cmH2O, EM4 0% is 40.6 cmH2O). Based on simulation using the chemical process simulator software, it is known that biogas heating value is 39,180 kJ/kg.</p>


2019 ◽  
Author(s):  
Christian Abendroth ◽  
Adriel Latorre- Pérez ◽  
Manuel Porcar ◽  
Claudia Simeonov ◽  
Olaf Luschnig ◽  
...  

AbstractConventional anaerobic digesters intended for the production of biogas usually operate in complete darkness. Therefore, little is known about the effect of light on microbial communities operating in anaerobic digesters. In the present work, we have studied through 16S rRNA gene amplicon Nanopore sequencing and shotgun metagenomic sequencing the taxonomic and functional structure of the microbial community forming a biofilm on the inner wall of a lab-scale transparent anaerobic biodigester illuminated with natural sunlight. The biofilm was composed of microorganisms involved in the four metabolic processes needed for biogas production. The biofilm proved surprisingly rich in Rhodopseudomonas faecalis, a versatile bacterium able to carry out a photoautotroph metabolism when grown under anaerobic conditions. Our results suggest that this bacterium, able to fix carbon dioxide, could be considered for its use in transparent biogas fermenters in order to contribute to the production of optimized biogas with a higher CH4:CO2 ratio than the biogas produced in regular, opaque digesters. To the best of our knowledge, this is the first study supporting illuminated bioreactors as a new bioprocess for the obtention of biogas enriched in methane.


Recycling ◽  
2019 ◽  
Vol 4 (1) ◽  
pp. 8 ◽  
Author(s):  
Jonathan Cardoso ◽  
Helder Gomes ◽  
Paulo Brito

The main environmental issue associated with compost production is the production of a liquid leachate. Leachate from municipal wastes contains carbon, nitrogen, phosphorus, potassium and trace elements that can be used as nutrients by plants. The advantages of the use of organic wastes such as compost leachate as fertilizers are evident. Their use would reduce the consumption of commercial fertilizers, which need, with their production, high cost and energy. This work aims to determine the physical and chemical properties of a specific leachate with a variable composition, collected from the composting line of a mechanical and biological treatment facility. The goal is to assess if the leachates can be used as a potential source for fertilizers, and thus develop and design a sequence of processes which could effectively convert the leachates to commercial fertilizers according to the requirements of the proposal of regulation of the European Parliament of 2016 for fertilizers. Preliminary results show that the leachate samples qualitatively meet the requirements established for the composition of commercial fertilizers, especially organo-mineral fertilizers. Furthermore, there is no production cost of leachate as a raw material. The results show that the leachate is characterized by manageable concentrations of heavy metals which can be removed by adsorption processes, and it presents suitable amounts of organic carbon after a water removal procedure. However, the establishment of the conditions for suitable conversion processes are still under investigation considering the high composition variability due to factors like storage and environmental conditions.


2019 ◽  
Vol 276 ◽  
pp. 06016
Author(s):  
Slamet Raharjo ◽  
Puti S. Komala ◽  
Adjar Pratoto

Current practice in Indonesia shows that Household Solid Waste (HSW) is handled by transferring to landfill site. Meanwhile, around 68% of HSW is composed of biodegradable components, which has potential for biogas production. The current study compares some commercial activators including Green Phosko (GP7) and Effective microorganisms 4 (EM4) with homemade activator in 200 L anaerobic digester equipped with manual mixing for processing kitchen waste (KW). pH and ratio of solid waste to water were set at 8 and 1:2, respectively. Batch experiments show that digester #3 with homemade activator shows superior performance compared to commercial activators. Meanwhile, the digesters with commercial activators show better performance compared to digester control (without activator). Digester #3 produces 1.35 L CH4/kg total solid (TS) and 1.14 L CH4/kg volatile solid (VS). Current work suggests that the homemade activator has the most suitable methanogen bacterium compared to the commercial activators.


Energies ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2843
Author(s):  
Krystyna Zielińska ◽  
Agata Fabiszewska ◽  
Katarzyna Piasecka-Jóźwiak ◽  
Renata Choińska

A new direction in the use of lactic acid bacteria inoculants is their application for renewable raw materials ensiling for biogas production. The aim of the study was to demonstrate the possibility of stimulating the synthesis of propionic acid in the process of co-fermentation of selected strains of Lactobacillus buchneri and L. diolivorans as well as L. buchneri and Pediococcus acidilactici. L. buchneri KKP 2047p and P. acidilactici KKP 2065p were characterized by the special capabilities for both synthesis and metabolism of 1,2-propanediol. L. diolivorans KKP 2057p stands out for the ability to metabolize 1,2-propanediol to propionic acid. As a result of the co-fermentation a concentration of propionic acid was obtained at least 1.5 times higher in the final stage of culture in comparison to cultivating individual species of bacteria separately. The results of in vitro experiments were applied in agricultural practice, by application of two lactic acid bacteria inoculants in ensiling of grass silage and improving its suitability for biogas production. Grass silages made with the addition of the inoculant were characterized by the content of 1,2-propanediol, 1-propanol and propionic acid ensured extension of the aerobic stability from 4 to 7 days in comparison to untreated silages. It was found that the use of both inoculants resulted in an approximately 10 - 30% increase in biogas yield from this raw material.


2021 ◽  
Vol 942 (1) ◽  
pp. 012008
Author(s):  
K Plawecka ◽  
B Figiela ◽  
A Grela ◽  
K E Buczkowska

Abstract Plasma waste treatment technology is one of the promising methods of reducing problematic waste, but the technology itself create other kind of waste that must be effectively used to recognize this technology as a suitable for circular economy. The aim of the article is to show the possibility of using waste materials from a plasma incineration plant as a raw material for the production of geopolymeric materials. The samples were made based on waste from plasma incineration plant in Liberec (Czech Republic), fly ash from thermal power plants in Skawina (Poland) and sand mixed with an aqueous chemical solution. Next they mechanical properties, microstructure and mineralogical composition have been investigated. The results show the combination of these raw materials can increase the strength of the material and, consequently find applications in the utilization of plasma incineration waste. The article show the geopolymerization could be effective process for using waste materials from a plasma incineration plant as a raw material for creating the products for construction industry.


2020 ◽  
Vol 2020 (08) ◽  
pp. 28-36
Author(s):  
Martina Zbašnik-Senegačnik ◽  
Ljudmila Koprivec

The built environment requires ever-increasing amounts of raw material resources and at the same time bears the responsibility for the resulting waste. Waste is generated throughout the life cycle. In the initial phases it is referred to as industrial waste, while during construction, reconstruction, and demolition it is called construction waste. Construction waste is most voluminous but it also has a great potential in circular economy that aims at the closed loop cycle where already used construction materials and components are recovered as raw materials. Sustainable building principles include four basic strategies, waste avoidance, construction materials and components re-use, continued use, and recycling. The possibility of construction waste treatment and its possible recovery in the building process depends on the type of prevailing materials that are contained in building elements as well as on detachability, separability and inseparability of structural joints and components. The architect plays a responsible role in decreasing the volume of construction waste as the conception of a building represents the key factor in sustainable construction waste management. Planning a construction with a good dismantling potential at the end of the building’s life cycle includes a number of factors such as the choice of building materials with a low environmental impact, the design of detachable composite materials and structures as well as the design of mono material structures. This article focuses on waste resulting from the built environment and discusses architectural concepts with a potential of reducing the volume of construction waste and its potential recovery as a construction resource.


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