Preparation, characterization and blood compatibility assessment of a novel electrospun nanocomposite comprising polyurethane and ayurvedic-indhulekha oil for tissue engineering applications

2018 ◽  
Vol 63 (3) ◽  
pp. 245-253 ◽  
Author(s):  
Manikandan Ayyar ◽  
Mohan Prasath Mani ◽  
Saravana Kumar Jaganathan ◽  
Rajasekar Rathanasamy
Keyword(s):  
Composite Scaffold ◽  
Blood Compatibility ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Weight Percentage ◽  
Force Microscopy ◽  
Hemolytic Assay ◽  
Measurement Analysis ◽  
The Fourier Transform ◽  
Nanocomposite Scaffolds

AbstractElectrospun polyurethane based nanocomposite scaffolds were fabricated by mixing with indhulekha oil. Scanning electron microscope (SEM) portrayed the nanofibrous nature of the composite and the average diameters of the composite scaffold were smaller than the pristine scaffolds. The fabricated scaffold was found to be hydrophobic (114°) due to the inclusion of indhulekha oil, which was displayed in contact angle measurement analysis. The fourier transform infrared spectroscopy (FTIR) results indicated that the indhulekha oil was dispersed in PU matrix identified by formation of hydrogen bond and peak shifting of CH group. The PU/indhulekha oil nanocomposite exhibits a higher decomposition onset temperature and also residual weight percentage at 900°C was more compared to the pure PU. Surface roughness was found to be increased in the composite compared to the pristine PU as indicated by the atomic force microscopy (AFM) analysis. In order to investigate the blood compatibility of electrospun nanocomposites the activated partial thromboplastin time (APTT) assay, prothrombin time (PT) assay and hemolytic assay were performed. The blood compatibility results APTT and PT revealed that the developed nanocomposites demonstrated delayed clotting time indicating the anticoagulant nature of the composite in comparison with the pristine PU. Further, it was also observed that the hemolytic index of nanocomposites was reduced compared to pure PU suggesting the non-hemolytic nature of the fabricated scaffold. Hence, the fabricated nanocomposites might be considered as a potent substitute for scaffolding damaged tissue due to their inherent physicochemical and blood compatibility properties.

Preparation and the Blood Compatibility of Titanium Oxide Nanorod Arrays

2011 ◽  
Vol 306-307 ◽  
pp. 25-30 ◽  
Author(s):  
Ping Luo ◽  
Zhan Yun Huang ◽  
Di Hu Chen
Keyword(s):  
Contact Angle ◽  
Surface Morphology ◽  
Titanium Oxide ◽  
Tin Oxide ◽  
Growth Parameters ◽  
Blood Compatibility ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Nanorod Arrays ◽  
Wetting Properties

In this work, titanium oxide nanorod arrays were fabricated by using the hydrothermal method on fluorine-doped tin oxide (FTO) coated glass. The diameter of the nanorods could be controlled from 150 nm to 30 nm by changing the growth parameters. The surface morphology and the structure of the samples were characterized by SEM and XRD. The wetting properties were identified by contact angle measurement. Platelet attachment was investigated to evaluate the blood compatibility of the samples with different nanoscale topographies. Results show that the nanotopographical surfaces perform outstanding blood compatibility, and the adhering platelet decreased with the increasing diameter of the nanorods.

scholarly journals Surface Properties of Alginate/Chitosan Biofilm for Wound Healing Application

2020 ◽  
Vol 1010 ◽  
pp. 602-607
Author(s):  
Maizlinda Izwana Idris ◽  
Mohammed Firdaus Adzhari ◽  
Siti Natrah Abdul Bakil ◽  
Tee Chuan Lee ◽  
Mohamad Ali Selimin ◽  
...  
Keyword(s):  
Contact Angle ◽  
Wound Healing ◽  
Surface Properties ◽  
Healing Process ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Force Microscopy ◽  
Good Surface ◽  
Atomic Force ◽  
Biopolymer Film

This work focuses on the fabrication of film based on natural biopolymers for wound healing application. Alginate and chitosan were choosen because of their oustanding properties such as biocompatible, hydrophilic and non-toxic. Earlier, the biopolymer film was fabricated by using alginate 1% wt and chitosan 1% wt. solutions at volume ratios of 99:1 and 97:3. Next, the biopolymer film solution was cross-linked with 1M CaCl2.2H2O for two hours and later dried for 24 hours at room temperature. Then, the surface properties of the prepared biopolymer films were characterised via Field Emission Scanning Electron Microscopy (FESEM), Atomic Force Microscopy (AFM) and contact angle measurement. It was observed that the surface of the biopolymer film became rougher as the volume of the chitosan increases. This condition was confirmed with average surface roughness, RA for biopolymer film with ratio of 97:3 resulted in higher values. Also it was found that the surface of biopolymer films were hydrophilic after the contact angle was less than 90°. This can be concluded that the biopolymer based on alginate/chitosan is a promising candidate for wound healing materials particularly with good surface properties for faster healing process at the wound areas.

scholarly journals Characterization of Lipid Membrane Properties for Tunable Electroporation

2012 ◽  
Author(s):  
H. Jeremy Cho ◽  
Shalabh C. Maroo ◽  
Evelyn N. Wang
Keyword(s):  
Lipid Bilayers ◽  
Lipid Membrane ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Membrane Properties ◽  
Force Microscopy ◽  
Packing Structure ◽  
Atomic Force ◽  
Dynamics Simulations

Lipid bilayers form nanopores on the application of an electric field. This process of electroporation can be utilized in different applications ranging from targeted drug delivery in cells to nano-gating membrane for engineering applications. However, the ease of electroporation is dependent on the surface energy of the lipid layers and thus directly related to the packing structure of the lipid molecules. 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) lipid monolayers were deposited on a mica substrate using the Langmuir-Blodgett (LB) technique at different packing densities and analyzed using atomic force microscopy (AFM). The wetting behavior of these monolayers was investigated by contact angle measurement and molecular dynamics simulations. It was found that an equilibrium packing density of liquid-condensed (LC) phase DPPC likely exists and that water molecules can penetrate the monolayer displacing the lipid molecules. The surface tension of the monolayer in air and water was obtained along with its breakthrough force.

scholarly journals Development of Nanoporous AAO Membrane for Nano Filtration Using the Acoustophoresis Method

Sensors ◽  
2020 ◽  
Vol 20 (14) ◽  
pp. 3833
Author(s):  
Yatinkumar Patel ◽  
Giedrius Janusas ◽  
Arvydas Palevicius ◽  
Andrius Vilkauskas
Keyword(s):  
Acoustic Waves ◽  
Chemical Properties ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Nanoporous Structure ◽  
Destructive Testing ◽  
Water Contact ◽  
Force Microscopy ◽  
Interpore Distance ◽  
Nanoporous Surface

A concept of a nanoporous anodic aluminum oxide (AAO) membrane as a vibro-active micro/nano-filter in a micro hydro mechanical system for the filtration, separation, and manipulation of bioparticles is reported in this paper. For the fabrication of a nanoporous AAO, a two-step mild anodization (MA) and hard anodization (HA) technique was used. Atomic force microscopy (AFM) and scanning electron microscopy (SEM) were used to analyze the surface morphology of nanoporous AAO. A nanoporous structure with a pore diameter in the range of 50–90 nm, an interpore distance of 110 nm, and an oxide layer thickness of 0.12 mm with 60.72% porosity was obtained. Fourier-transform infrared spectroscopy (FTIR) and energy-dispersive X-ray spectroscopy (EDS) were employed to evaluate AAO chemical properties. The obtained results showed that the AAO structure is of hexagonal symmetry and showed where Al2O3 is dominant. The hydrophobic properties of the nanoporous surface were characterized by water contact angle measurement. It was observed that the surface of the nanoporous AAO membrane is hydrophilic. Furthermore, to determine whether a nanomembrane could function as a vibro-active nano filter, a numerical simulation was performed using COMSOL Multiphysics 5.4 (COMSOL Inc, Stockholm, Sweden). Here, a membrane was excited at a frequency range of 0–100 kHz for surface acoustics wave (SAW) distribution on the surface of the nanoporous AAO using a PZT 5H cylinder (Piezo Hannas, Wuhan, China). The SAW, standing acoustic waves, and travelling acoustic waves of different wavelengths were excited to the fabricated AAO membrane and the results were compared with experimental ones, obtained from non-destructive testing method 3D scanning vibrometer (PSV-500-3D-HV, Polytec GmbH, Waldbronn, Germany) and holographic interferometry system (PRISM, Hy-Tech Forming Systems (USA), Phoenix, AZ, USA). Finally, a simulation of a single nanotube was performed to analyze the acoustic pressure distribution and time, needed to center nanoparticles in the nanotube.

Green Polyurethane Adhesives for Wood Bonding

2015 ◽  
Vol 1115 ◽  
pp. 331-336
Author(s):  
M.A.F. Amran ◽  
Ernie Suzana Ali
Keyword(s):  
Untreated Wood ◽  
Adhesive System ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Lap Shear ◽  
Lap Shear Test ◽  
Wood Joint ◽  
Wood Bonding ◽  
Polyurethane Adhesives ◽  
The Fourier Transform

The Green polyurethane adhesive system was prepared using two types palm oil polyol having different molecular weight ~1100 (GA) and ~ 2200 (GB) respectively, 4-4-diphenylmethane diisocyanate (MDI) and nanoclay fillers via in-situ process. Adhesives were applied on both sides of two untreated wood substrate using brushing technique for lap shear test. The attached substrate then undergoes the curing process for 24 hours at room temperature with relative humidity of 50±5%. The disappearance of NCO peak in the Fourier transform infra-red (FTIR) spectrum showed that MDI has completely reacted to form PU. Contact angle measurement proved that high wetting condition obtains from green polyurethane (PU) adhesive. PU adhesive wood joint improved in shear strength with addition of 1wt% nanoclay for both green polyol.

Topographical Evolution of Nanocrystalline Diamond and Its Effect on Osteoblast Interactions

2007 ◽  
Vol 1039 ◽  
Author(s):  
Lei Yang ◽  
Brian W Sheldon ◽  
Thomas J Webster
Keyword(s):  
Grain Size ◽  
Cell Adhesion ◽  
Microwave Plasma ◽  
Chemical Vapor ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Cellular Adhesion ◽  
Nanocrystalline Diamond ◽  
Sem Images ◽  
Force Microscopy

AbstractThe potential of nanocrystalline diamond films (NDFs) for biological applications has been addressed by a variety of recent researchers. In the present work, we consider the topographical evolution of NDFs fabricated by microwave plasma assisted chemical-vapor-deposition (MPCVD) and its influence on osteoblast (bone forming cells) functions. One group of NDFs formed in this study were grown with 0.67% methane, 5%∼20% hydrogen and argon. The other group of NDFs composed of nano diamond and amorphous carbon was created with indirect exposure to plasma with 0.67% methane, 20% hydrogen and argon. Scanning electron microscope (SEM) images revealed that the nano diamond grains in the first group of NDFs evolved from round shapes into faceted and successively cubic shapes as hydrogen increased, whereas the second group of NDFs consisted of nano platelet grains. Atomic force microscopy (AFM) analysis confirmed this evolution as well as the variation of surface roughness. Cell results demonstrated that osteoblast adhesion and proliferation on NDFs varied dramatically depending on the different topographical features of the films. Specifically, results showed that NDFs with grain size less than 100 nm could be coated on the stem of a hip implant to promote cell adhesion. In contrast, NDFs with grain size greater than 200 nm or NDF with nano platelet grains may be optimal for the inside of acetabular articulation where cell adhesion is not preferred but wear-resistance is important. Further observation by contact angle measurement suggested that the differences in cellular adhesion and proliferation were related to the wettability associated to the topographical features of NDFs surfaces.

scholarly journals Evaluation of Antithrombogenic pHPC on CoCr Substrates for Biomedical Applications

Coatings ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 93
Author(s):  
Catrin Bannewitz ◽  
Tim Lenz-Habijan ◽  
Jonathan Lentz ◽  
Marcus Peters ◽  
Volker Trösken ◽  
...  
Keyword(s):  
Contact Angle ◽  
Platelet Adhesion ◽  
Significant Risk ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Force Microscopy ◽  
Covered Area ◽  
Chandler Loop ◽  
Coated Surfaces

Bare metal endovascular implants pose a significant risk of causing thrombogenic complications. Antithrombogenic surface modifications, such as phenox’s “Hydrophilic Polymer Coating” (pHPC), which was originally developed for NiTi implants, decrease the thrombogenicity of metal surfaces. In this study, the transferability of pHPC onto biomedical CoCr-based alloys is examined. Coated surfaces were characterized via contact-angle measurement and atomic force microscopy. The equivalence of the antithrombogenic effect in contact with whole human blood was demonstrated in vitro for CoCr plates compared to NiTi plates on a platform shaker and for braided devices in a Chandler loop. Platelet adhesion was assessed via scanning electron microscopy and fluorescence microscopy. The coating efficiency of pHPC on CoCr plates was confirmed by a reduction of the contact angle from 84.4° ± 5.1° to 36.2° ± 5.2°. The surface roughness was not affected by the application of pHPC. Platelet adhesion was significantly reduced on pHPC-coated specimens. The platelet covered area was reduced by 85% for coated CoCr plates compared to uncoated samples. Uncoated braided devices were completely covered by platelets, while on the pHPC-coated samples, very few platelets were visible. In conclusion, the antithrombogenic effect of pHPC coating can be successfully applied on CoCr plates as well as stent-like CoCr braids.

scholarly journals Hyaluronan Immobilized Polyurethane as a Blood Contacting Material

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Feirong Gong ◽  
Yue Lu ◽  
Hui Guo ◽  
Shujun Cheng ◽  
Yun Gao
Keyword(s):  
Blood Compatibility ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Total Reflection ◽  
Attenuated Total Reflection ◽  
Chemical Compositions ◽  
Coagulation Time ◽  
Water Contact ◽  
Excess Amount ◽  
Surface Changes

Hyaluronan (hyaluronic acid, HA) was immobilized onto the surface of amino-functionalized polyurethane films with the goal of obtaining a novel kind of biomaterial which had the potential in blood-contacting applications. The amino-functionalized polyurethane was prepared by synthesized acidic polyurethane whose pendant carboxyl groups were treated with an excess amount of 1,3-diaminopropane in the presence of N,N-carbonyldiimidazole (CDI). Attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), Raman spectroscopy (RS), scanning electron microscopy (SEM), and water contact angle measurement were used to confirm the surface changes at each step of treatment, both in morphologies and chemical compositions. APTT and PT results showed that HA immobilization could prolong the blood coagulation time, thus HA-immobilized polyurethane (PU-HA) exhibited improved blood compatibility. Cytotoxicity analysis showed that the PU-HA films synthesized in this study were cytocompatible and could support human vein endothelial cells (HUVECs) adhesion and proliferation.

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