Physicochemical analysis, proteolysis activity and exopolysaccharides production of herbal yogurt fortified with plant extracts

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Amal Bakr Shori ◽  
Chin Wai Peng ◽  
Elham Bagheri ◽  
Ahmad Salihin Baba
Keyword(s):  
Starter Culture ◽  
Water Extract ◽  
Milk Proteins ◽  
Streptococcus Thermophilus ◽  
Psidium Guajava ◽  
Physicochemical Analysis ◽  
Plant Water ◽  
Garcinia Mangostana ◽  
Lycium Barbarum ◽  
Water Extracts

AbstractYogurt production with starter culture at 41 °C in the presence of plant water extracts (Momordica grosvenori, Psidium guajava, Lycium barbarum or Garcinia mangostana) were studied to examine the effects on acidification, physicochemical properties, microbial growth, proteolytic activity, and exopolysaccharide (EPS) content. All plant-based yogurt reached a pH of 4.5 faster (300–330 min) than plain-yogurt (360 min). All plant water extracts stimulated Lactobacillus spp. (∼7.4 log10 CFU/mL) and Streptococcus thermophilus (8.20–8.50 log10 CFU/mL) growth except for G. mangostana which marginally inhibited Lactobacillus spp. growth (7.21 log10 CFU/mL). M. grosvenori, L. barbarum, and G. mangonstana were significantly affected proteolysis of milk proteins (46.2 ± 0.8, 39.9 ± 0.5, & 35.8 ± 0.1 µg/mL; respectively) compared to plain-yogurt (26.3 ± 0.4 µg/mL). The presence of G. mangostana and L. barbarum resulted in an increase (p < 0.05) of total solids content (∼15.0%) and water holding capacity in yogurt (28.1 ± 1.2 & 26.5 ± 0.3%; respectively; p < 0.05). In addition, M. grosvenori water extract enhanced (p < 0.05) syneresis of yogurt (1.78 ± 0.30%). L. barbarum yogurt showed the highest EPS concentration (220.9 ± 12.4 µg/L) among yogurt samples. In conclusion, the presence of plant water extracts positively altered yogurt fermentation, enhanced proteolysis of milk protein, and induced EPS production.

scholarly journals Effects of Garcinia mongostana, Lycium barbzarum, Momordica grosvenori, and Psidium guajava on the Growth of Lactobacillus spp. and Streptococcus thermophilus

2021 ◽  
pp. 30-39
Author(s):  
Elham Bagheri ◽  
Amal Bakr Shori ◽  
Ahmad Salihin Baba
Keyword(s):  
Water Extract ◽  
Streptococcus Thermophilus ◽  
Psidium Guajava ◽  
Herbal Extract ◽  
Plant Water ◽  
Inhibitory Effects ◽  
Lycium Barbarum ◽  
Fermented Beverage ◽  
Dose Response Effect ◽  
Lactobacillus Spp

There are an increasing number of fermented beverage using herbal extract as a natural ingredient. The growth of bacteria in four plant water extract (Lycium barbarum, Psidium guajava, Momordica grosvenori, and Garcinia mongostana) as measured by the increase in turbidity of MRS and M17 growth medium containing Lactobacillus spp. and Streptococcus thermophilus respectively was investigated during 30 minutes of incubation at 37°C. The stimulatory effects on Lactobacillus spp. growth was tremendously enhanced (p<0.05) by L. barbarum (3.0%; OD=0.1.1) and P. guajava (1.5% (OD=0.5) and 3.0% (OD=0.6); respectively) compared to control (0%) after 30 minutes. In addition, inclusion of P. guajava (1.5%) shorted incubation time to reach plateau at 5 minutes. The inclusion of M. grosvenori and G. mangostana water extract at 0.30% increased Lactobacillus spp. growth by 2 and 14 fold respectively which higher than control. There was a dose response effect of all plant water extracts except for M. grosvenori on the growth of S. thermophilus. All the three concentrations of L. barbarum and P. guajava stimulated the growth of S. thermophilus which reach 3 fold higher than control at 1.5% L. barbarum. On the other hand, M. grosvenori water extract showed inhibitory effects on the growth of S. thermophilus at all doses but after the first 15 min at 3.0% the inhibitory effects were lost (p<0.05). G. mangostana water extract at 3.0% increased S. thermophilus growth 10 fold higher than control after 30 minutes. In conclusion, all plant water extract samples except M. grosvenori could be a good vehicle for carrying Lactobacillus ssp. and S. thermophilus while M. grosvenori could enhance the growth of Lactobacillus spp. but not S. thermophilus.

scholarly journals Growth Rate of Lactobacillus ssp. and Streptococcus thermophilus of some Medicinal Plants Water Extracts with Fish Collagen

2021 ◽  
Vol 12 (1) ◽  
pp. 824-832
Keyword(s):  
Growth Rate ◽  
Bacterial Growth ◽  
Water Extract ◽  
Streptococcus Thermophilus ◽  
Plant Water ◽  
Lycium Barbarum ◽  
Fish Collagen ◽  
Illicium Verum ◽  
Presence And Absence ◽  
Lactobacillus Spp

The main objective of this study was to evaluate the growth rate of Lactobacillus ssp. and Streptococcus thermophilus in 6 types of plant water extract (Lycium barbarum, Illicium verum, Psidium guajava, Curcuma longa, Allium sativum, and Codonopsis pilosula) with two different concentration (10% and 2.5%; w/v) both in the presence and absence of fish collagen as a protein source during incubation at 37°C. The growth rate was measured using a spectrophotometer, and the absorbance was taken every 4 hours for 28 hours (S. thermophilus) and 48 hours (Lactobacillus spp.). The effects of plant water extract on bacterial growth were dose-dependent. The growth of S. thermophilus and Lactobacillus spp. in all samples increased with incubation time. After about 12 hours for S. thermophilus in 2.5% plant extract and 16 hours for other samples with or without fish collagen, it began to plateau. The growth rate of Lactobacillus spp. was significantly higher (p<0.05) than S. thermophilus. Illicium verum and Lycium barbarum, both in the presence and absence of fish collagen, showed the most significant influence of bacterial growth among other samples. Fish collagen had a slight effect on bacteria growth during the incubation period. In conclusion, all plant samples could be an effective vehicle for carrying Lactobacillus ssp. and S. thermophilus.

scholarly journals Role of growth regulators and plant water extracts on the productivity and water use efficiency of wheat genotypes under limited water supply

2019 ◽  
Vol 35 (6) ◽  
Author(s):  
Safdar Hussain ◽  
Abdul Rehman ◽  
Muhammad Akram ◽  
Javaid Iqbal ◽  
Tahira Batool Qaisrani ◽  
...  
Keyword(s):  
Growth Regulators ◽  
Block Design ◽  
Compatible Solutes ◽  
Water Extract ◽  
Grain Filling ◽  
Plant Water ◽  
Wheat Genotypes ◽  
Water Extracts ◽  
Filling Stage ◽  
Grain Filling Stage

Empowerment of wheat genotypes by application of growth regulators, compatible solutes and plant extracts under water restriction is an important strategy for getting sustainable yield. Field trail was performed at the research area of the Faculty of Agricultural Sciences, Ghazi University, Dera Ghazi Khan, Pakistan. Three wheat cultivars Aas-2011, Faisalabad- 2008 and Triple dwarf-1 were subjected to drought stress (skipping the irrigation at grain filling stage). The wheat genotypes were subjected to treatments viz., T1 i.e. All normal irrigation without application of abscisic acid (ABA), salicylic acid (SA), moringa (MLE) and mulberry leaf water extract (MBLE), T2­ i.e. skipping the irrigation at grain filling stage and application of 2µM ABA, T3 i.e. skipping the irrigation at grain filling stage and application of 10 m mol SA, T4 i.e. skipping the irrigation at grain filling stage and application of 15% MLE and T5 i.e. skipping the irrigation at grain filling stage and application of 10% MBLE. The experiment was laid out in Randomized Complete Block Design with factorial arrangement and repeated three times. From this study it is concluded that Aas-2011 shown best result under drought condition by applying growth regulators and plant water extracts. rs and plant water extracts.

scholarly journals Biofilm Formation on Stainless Steel by Streptococcus thermophilus UC8547 in Milk Environments Is Mediated by the Proteinase PrtS

2017 ◽  
Vol 83 (8) ◽  
Author(s):  
D. Bassi ◽  
F. Cappa ◽  
S. Gazzola ◽  
L. Orrù ◽  
P. S. Cocconcelli
Keyword(s):  
Stainless Steel ◽  
Biofilm Formation ◽  
Starter Culture ◽  
High Capacity ◽  
Milk Proteins ◽  
Streptococcus Thermophilus ◽  
Content Type ◽  
Factors Affecting ◽  
Ecological Benefit ◽  
Cell Envelopes

ABSTRACT In Streptococcus thermophilus, gene transfer events and loss of ancestral traits over the years contribute to its high level of adaptation to milk environments. Biofilm formation capacity, a phenotype that is lost in the majority of strains, plays a role in persistence in dairy environments, such as milk pasteurization and cheese manufacturing plants. To investigate this property, we have studied S. thermophilus UC8547, a fast-acidifying dairy starter culture selected for its high capacity to form biofilm on stainless steel under environmental conditions resembling the dairy environment. Using a dynamic flow cell apparatus, it was shown that S. thermophilus UC8547 biofilm formation on stainless steel depends on the presence of milk proteins. From this strain, which harbors the prtS gene for the cell wall protease and shows an aggregative phenotype, spontaneous mutants with impaired biofilm capacity can be isolated at high frequency. These mutants lack the PrtS expendable island, as confirmed by comparison of the genome sequence of UC8547Δ3 with that of the parent strain. The prtS island excision occurs between two 26-bp direct repeats located in the two copies of the ISSth1 flanking this genomic island. The central role of PrtS was confirmed by analyzing the derivative strain UC8547Δ16, whose prtS gene was interrupted by an insertional mutation, thereby making it incapable of biofilm formation. PrtS, acting as a binding substance between the milk proteins adhered to stainless steel and S. thermophilus cell envelopes, mediates biofilm formation in dairy environments. This feature provides S. thermophilus with an ecological benefit for its survival and persistence in this environment. IMPORTANCE The increased persistence of S. thermophilus biofilm has consequences in the dairy environment: if, on the one hand, the release of this microorganism from biofilm can promote the fermentation of artisanal cheeses, under industrial conditions it may lead to undesirable contamination of dairy products. The study of the molecular mechanism driving S. thermophilus biofilm formation provides increased knowledge on how an ancestral trait affects relevant phenotypes, such as persistence in the environment and efficiency of growth in milk. This study provides insight into the genetic factors affecting biofilm formation at dairy plants.

scholarly journals Allelopathic Effects of Different Plant Water Extracts on Yield and Weeds of Wheat

2018 ◽  
Vol 36 (0) ◽  
Author(s):  
M. NAEEM ◽  
Z.A. CHEEMA ◽  
M.Z. IHSAN ◽  
Y. HUSSAIN ◽  
A. MAZARI ◽  
...  
Keyword(s):  
Chenopodium Album ◽  
Substantial Reduction ◽  
Water Extract ◽  
Rate Of Return ◽  
Control Treatment ◽  
Plant Water ◽  
Marginal Rate ◽  
Water Extracts ◽  
Phytotoxic Effect ◽  
Marginal Rate Of Return

ABSTRACT: Use of synthetic herbicides to control weeds poses serious threats to sustainable agricultural production around the globe. The phytotoxic effect of different plant water extracts (i.e., sorghum, sunflower and mulberry) against weeds has been previously studied; however, their combined effects at different rates are yet to be explored. In this study, mixtures of allelopathic water extracts of sorghum + sunflower and sorghum + sunflower + mulberry were sprayed each at 12, 15, 18 and 21 L ha-1 at 40 and 55 days after sowing in wheat to control wild oats (Avena fatua), little seed canary grass (Phalaris minor), lamb’s quarters (Chenopodium album) and swine cress (Coronopus didymus). A synthetic herbicide Affinity 50 WP (Carfentrazone-ethyl 0.75% + Isoproturon 50% applied at 1000 g a.i., ha-1) and a weedy check were kept as a control treatment. The increasing order of water extract dose 12-21 L ha-1 showed substantial reduction in total weed density and their dry biomass production of both narrow and broad-leaved weeds in all the treatment combinations. Mixture of sorghum + sunflower + mulberry water extracts each at 18 L ha-1 applied at 40+55 DAS resulted in an 87.14% decrease in total weed dry matter with a 19.5% increase in grain yield of wheat and higher net benefits of Rs. 100525 with 777.5% marginal rate of return. Nonetheless, application of sorghum + sunflower water extracts each at 12 L ha-1 at 40+55 DAS was more economical than the other treatments in terms of higher marginal rate of return of 799.82%. Based on this study, it can be suggested that the use of allelopathic plant water extracts in a mixture may act as a potential weed control strategy in wheat.

scholarly journals Combined Application of Natural Plant Water Extracts and Biochar Improves the Productivity of Bread Wheat

2018 ◽  
Author(s):  
Muhammad Ijaz ◽  
Sonia Parveen ◽  
Sajid Ali ◽  
Ahmad Nawaz ◽  
Ahmad Sher ◽  
...  
Keyword(s):  
Grain Yield ◽  
Bread Wheat ◽  
Management Strategies ◽  
Winter Season ◽  
Water Extract ◽  
Control Treatment ◽  
Combine Application ◽  
Plant Water ◽  
Natural Plant ◽  
Water Extracts

Bread wheat (Triticum aestivum L.) is staple of Pakistani people. However, its yield at farmer field is low as compared with its genetic potential. Integration of various crop and soil management strategies might be an option to enhance wheat productivity at farmer field. This 2-year experiment was conducted to check the influence of combine application of natural plant water extracts and biochar on the productvity of wheat during the winter season of 2015-16. The experiment consisted of seven treatment viz. (1) control (2) application of biochar (0.18 kg pot-1) alone, (3) application of sorghum water extract (SWE) alone, (4) application of moringa water extract (MWE) alone, (5) application of biochar + SWE, (6) application of biochar + MWE, (7) application of biochar + SWE+MWE. The results revealed that application of both crop water extracts in combination with biochar improved the growth and grain yield of wheat. Use of MWE in combination with biochar enhanced the grain weight, grain number and grain yield of wheat by 44, 14, and 24%, respectively than the control treatment. In crux, use of MWE in combination with biochar might be a viable option to improve the productivity of bread wheat.

scholarly journals Antioxidant properties of hot water extracts from carpophore and spores of mushroom Ganoderma lucidum

2011 ◽  
pp. 279-288 ◽  
Author(s):  
Anita Klaus ◽  
Maja Kozarski ◽  
Miomir Niksic
Keyword(s):  
Antioxidant Activity ◽  
Ganoderma Lucidum ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Reducing Power ◽  
Hot Water ◽  
Ferrous Ions ◽  
Medicinal Mushrooms ◽  
Water Extracts ◽  
Hot Water Extract

Ganoderma lucidum (Leyss.:Fr.) Karst is one of the medicinal mushrooms, which possesses enviable antioxidant properties. Objective of this investigation was to evaluate antioxidant activity, reducing power, scavenging abilities on 1.1-diphenyl-2-picrylhydrazyl (DPPH) radicals and chelating effects on ferrous ions of hot water extracts obtained from carpophore and spores of this mushroom. Hot water extract from carpophore (Gl-I) showed high antioxidant activity of 85.7 ? 0.7%, at 10 mg/ml, while antioxidant activity of hot water extract from spores (Gl-Is) was 9.2 ? 0.3% at 10 mg/ml. Reducing power of Gl-I reached a plateau of 3.4 ? 0.1 at 20 mg/ml, and 0.3 ? 0.0 at 20 mg/ml for Gl-Is. At 10 mg/ml, scavenging ability on DPPH radicals of Gl-I increased to 96.8 ? 2.5%, whereas Gl-Is scavenged DPPH radicals by 69.6 ? 2.5% at 10 mg/ml. Gl-I chelated 81.6 ? 3.6 % of ferrous ions at 20 mg/ml, while the chelating effect of Gl-Is was 73.8 ? 1.7%. The antioxidative activities of hot water extracts from carpophore and spores of the mushroom G. lucidum were concentration dependent and increased with an increase in the concentration.

Inhibitory Activity of Extracts from Mycelia Materials of Several Mushrooms on the Angiotensin I-Converting Enzyme

2012 ◽  
Vol 610-613 ◽  
pp. 3541-3544
Author(s):  
Pei Sheng Yan ◽  
Xiu Jun Gao
Keyword(s):  
Liquid Medium ◽  
Inhibitory Activity ◽  
Water Extract ◽  
Angiotensin I ◽  
Water Extracts ◽  
Angiotensin I Converting Enzyme ◽  
Ic50 Value ◽  
Ic50 Values ◽  
The Difference ◽  
Ace Inhibitory

ACE inhibitory activities of water extracts from mycelia of 6 kinds of mushrooms in liquid fermentation were investigated. All strains cultivated in shaking flask containing liquid medium, and yield of mycelia ranged from 0.051 to 1.392 g mycelia/day/L liquid medium in average. Resulting mycelia were extracted with distilled water at 50°Cfor 200 min, and the yield of water extracts from mycelia ranged from 287.475 to 490.088 mg/g dried mycelia. These water extracts were used to assay their ACE inhibitory activity. Results showed that their IC50 values ranged from 1.277 to 5.250 mg/ml. The difference among IC50 values of these water extracts were significant (p<0.05 or p<0.001). Lactarius camphorates (IC50: 1.646±0.061mg/mL) was the specie which had relatively lower IC50 value than others, as well as relatively higher water extract yield. The results highlighted the potential for making antihypertensive functional foods or drugs from liquid cultured mycelia of Lactarius camphorates.

scholarly journals Usages of Salvia officinalis Boiled Water Extract for Pathogenic Escherichia coli Exclusion at Elevated Region

2021 ◽  
Vol 2 (3) ◽  
pp. 01-03
Author(s):  
Sherifa Sabra
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Water Extract ◽  
Salvia Officinalis ◽  
Bacterial Suspension ◽  
Water Extracts ◽  
Pathogenic Escherichia Coli ◽  
Chemical Content ◽  
Boiled Water ◽  
Chemical Contents

The aim was for tryout using daily regular Salvia officinalis boiled water extracts in "Arab and Saudi Society", especially at elevated region "Taif". That to prove its ability for pathogenic Escherichia coli exclusion and had antibiotic resistance. This could prove strength of chemical content and adherence of "Arab People" to use as regular and daily to stabilize body health and protect against pathogenic bacteria. Salvia officinalis was purchased from pharmacy at "Taif" and boiled water extract was made. Pathogenic Escherichia coli was obtained from a "Licensed Health Center"; culture was made and suspensions. Tryout by boiled water extract were added to bacterial suspension and then were kept in incubator. The samples were taken at hours (1, 3, 5, 7, 9, 11, 13, 15, 17 and 19); were cultured and were kept for 48 hours at 37 °C. The exclusion percent's had determined, Escherichia coli exclusion mean percentages rose 5% at third hour and then increased 7% at fifth hour and at ninth hour was 8%. Then it decreased at the eleventh hour and thirteenth hour (2% and 3%), then rose at the fifteenth hour 10%. It decreased 5% at the seventeenth hour, and then returned and rose at the nineteenth hour 12%. It was concluded Salvia officinalis boiled water extracts effects on pathogenic Escherichia coli exclusion was found during one growth bacteria cycle, indicated the characteristics of chemical contents and good daily habit. It was recommend continuing as "Arabian Habit" Salvia officinalis boiled water extracts for health and protection stability.

scholarly journals Effect of Natural Plant Water Extracts Suppressed Melanin Production in B16 Mouse Melanoma Cell

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Tae Hyuk Kim ◽  
Mi Ja Chung ◽  
Dae Jung Kim ◽  
Jin Kyoun You ◽  
Dong Joo Seo ◽  
...  
Keyword(s):  
Melanoma Cell ◽  
Plant Water ◽  
Melanin Production ◽  
Natural Plant ◽  
Mouse Melanoma ◽  
Water Extracts ◽  
Mouse Melanoma Cell ◽  
B16 Mouse Melanoma
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