Effect of curcumin analogue synthetic product from cullilawan oil for the liver damage treatment in male mice (Mus musculus L.)

2020 ◽  
Vol 30 (6) ◽  
Author(s):  
Imanuel Berly Delvis Kapelle ◽  
Wasmen Manalu ◽  
Fensia Analda Souhoka
Keyword(s):  
Liver Damage ◽  
Mus Musculus ◽  
Liver Cells ◽  
Synthesis Process ◽  
Male Mice ◽  
Curcumin Analogue ◽  
Synthetic Product ◽  
Blood Biochemical ◽  
Histopathological Images

AbstractThe active component in cullilawan oil can be synthesized into curcumin analogue product, which has pharmacological activity. The synthesis process by using conventional and microwave methods can produce different isomer products. Different synthesis products and models of animal are used to provide different hepatoprotective effects. The aim of this study was to use the curcumin analogue synthetic products (AKS-k and AKS-m) from cullilawan oil in male mice (Mus musculus L.) liver damage treatment induced by carbon tetrachloride (CCl4). The in vivo method was employed using biochemical of blood and histopathological images of liver cells as indicators. The results showed that the curcumin analogue synthetic product using microwave methods had better pharmacological effects than the conventional method product in terms of the results of blood biochemical analysis and microscopic images of liver cells.

Influence of Process Methods on the Hepatoprotective Effect of Curcumin Analogs Synthesized from Culilawan Oil in Mice (Mus musculus L.) with CCl4 Induced Liver Damage

2019 ◽  
Vol 119 (2) ◽  
pp. 28
Author(s):  
Imanuel B. D. Kapelle ◽  
Wasmen Manalu ◽  
Meillisa C. Mainassy
Keyword(s):  
Conventional Method ◽  
Liver Damage ◽  
Mus Musculus ◽  
Aldol Condensation ◽  
Synthesis Process ◽  
Microwave Method ◽  
Pharmacological Effects ◽  
Curcumin Analogues ◽  
Curcumin Analogs

One of the downstream products which can be synthesized from culilawan oil is an analog curcumin compound (AKS) with a dioxolane ring. AKS products can be synthesized using conventional and microwave methods. The method of synthesis can influence physical properties, compound geometry, and pharmacological effects. The purpose of this study was to determine the effect of the processing method on the hepatoprotective ability of AKS, and to determine a protective dose. AKS was synthesized using insulated safrole compounds from Lawang oil and involved isomerization, oxidation, and aldol condensation of curcumin analogues. At the final stage of the analog curcumin synthesis process, 2 different methods were employed: the conventional method heated the chemical in a water bath at 30 °C for 3 hours, the microwave method heated the chemical using 140 watts of power for 2 minutes. Analogs were tested in vivo in mice (Mus musculus L.) with CCl4 induced liver damage. Hepatoprotective efficacy of AKS products processed by the conventional method and the microwave method were compared using histology and liver enzyme (AST and ALT) assessment. Animals treated with conventionally produced AKS products had lower AST and lower ALT levels—and fewer histological signs of liver damage at a lower dose of AKS—than seen in either untreated animals or those treated with microwave produced AKS. Thus, products that are processed by conventional methods are more hepatoprotective.

scholarly journals PENGARUH METODE PROSES SINTESIS ANALOG KURKUMIN ASIMETRIS TERHADAP EFEK HEPATOPROTEKTIF MENCIT (Mus musculus L.)

2020 ◽  
Vol 7 (2) ◽  
pp. 215-225
Author(s):  
Imanuel Berly Delvis Kapelle ◽  
Wasmen Manalu
Keyword(s):  
Liver Damage ◽  
Mus Musculus ◽  
Histological Analysis ◽  
Product Diversification ◽  
Synthesis Process ◽  
Synthesis Methods ◽  
Curcumin Analog ◽  
Curcumin Analogs ◽  
Process Method

Effect of Synthesis Process Method of Asymmetric Curcumin Analog on the Hepatoprotective Effect of Mice (Mus musculus L.) Asymmetric curcumin analogs (ACA) can be synthesized from cullilawan oil. ACA products can be synthesized using conventional methods and microwaves. Synthesis methods can affect physical properties and pharmacological effects. The purpose of this study was to determine the effect of the process method on the hepatoprotective ability of ACA and to determine the effective dose. ACA products were tested in vivo in mice (Mus musculus L.) with CCl4-induced liver damage. The parameters observed were biochemical liver enzymes (AST and ALT) and histological analysis. The results showed that animals treated with ACA-k products had better weight gain, lower AST and ALT levels, and fewer histological signs of liver damage at higher ACA doses than those observed in animals that were untreated or treated with ACA-m products. Thus, asymmetric curcumin analog products that were processed by conventional method were more hepatoprotective. Keywords: ACA, culilawan oil, hepatoprotection, in vivo, product diversification ABSTRAK Analog kurkumin asimetris (AKAS) dapat disintesis dari minyak kulit lawang. Produk AKAS dapat disintesis menggunakan metode konvensional dan microwave. Metode sintesis dapat mempengaruhi sifat fisik dan efek farmakologis. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh metode proses pada kemampuan hepatoprotektif AKAS dan untuk menentukan dosis efektif. Produk AKAS diuji in vivo pada mencit (Mus musculus L.) dengan kerusakan hati yang diinduksi CCl­4. Parameter yang diamati adalah biokimia enzim hati (AST dan ALT) dan analisis histologis. Hasil penelitian menunjukkan bahwa hewan yang diobati dengan produk AKAS-k memiliki kenaikan berat badan yang lebih baik, tingkat AST dan ALT yang lebih rendah, dan lebih sedikit tanda histologis kerusakan hati pada dosis AKAS yang lebih tinggi daripada yang terlihat pada hewan yang tidak diperlakukan atau diobati dengan produk AKAS-m. Dengan demikian, produk analog kurkumin asimetris yang diproses dengan metode konvensional lebih hepatoprotektif.

scholarly journals Correlation between Antistress and Hepatoprotective Effects of Schisandra Lignans Was Related with Its Antioxidative Actions in Liver Cells

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Hao-Jie Pu ◽  
Yun-Feng Cao ◽  
Rong-Rong He ◽  
Zhi-Long Zhao ◽  
Jin-Hui Song ◽  
...  
Keyword(s):  
Free Radicals ◽  
Liver Damage ◽  
Restraint Stress ◽  
Liver Cells ◽  
Protective Effects ◽  
Male Mice ◽  
Stress Effects ◽  
Restraint Tube ◽  
Hepatoprotective Effects ◽  
The Relationship

The present study was conducted to investigate the relationship between the anti-stress and hepato-protective effects of Schisandra Lignans Extract (SLE) on stress-induced liver damage. Seven weeks old male mice were fixed in a restraint tube for 18 h to induce liver damage. SLE was orally administered to animals for 5 days at dosages of 100 and 200 mg/kg/day before exposed to restraint stress. Oral administration of SLE significantly reduced restraint-induced liver damage in experimental animal. SLE was further found to significantly alleviate the provocation of corticosterone in stressed mice. SLE also significantly decreased oxidative damage and increased anti-oxidative capability of liver cells by preventing the over production and accumulation of free radicals. In conclusion, the protective effects of SLE on stress-induced liver damage were confirmed, and the correlation between hepatoprotective and anti-stress effects of schisandra lignans was possible related to its alleviation on the malignant effects of stressors for bio-homeostasis, such as balance of oxidation and reduction in cells.

scholarly journals KAJIAN EFEKTIVITAS EKSTRAK GEL IKAN TOMAN (Channa micropeltes) PADA LUKA BAKAR MENCIT (Mus musculus)

2019 ◽  
Vol 15 (2) ◽  
pp. 184
Author(s):  
Firlianty Firlianty ◽  
Hermansyah Hermansyah ◽  
Windarina Samosir
Keyword(s):  
Data Analysis ◽  
Experimental Method ◽  
Mus Musculus ◽  
Control Treatment ◽  
Test Results ◽  
Male Mice ◽  
In Vivo Test ◽  
Randomized Design ◽  
Completely Randomized Design

This study aims to determine the study of the effectiveness of toman fish (Channa micropeltes) gel extract on healing burns of mice (Mus musculus). This research was conducted for 4 months, starting from September 2018 to January 2019. This study used the experimental method and data analysis using a completely randomized design with 4 children and three replications: treatment A as control, treatment B giving toman fish extract gel (Channa micropeltes ) 1.5%, treatment C 3% and treatment D 4.5%. The results showed that on day 4, day 8 and day 12 showed that the administration of gel extract had a significant effect on changes in the length of burns of mice (Mus musculus) (P>0.05). In vivo test results on male mice with toman fish gel extract can capture the healing of burns in mice.

Uptake, Internalization and Degradation of the Novel Plasminogen Activator Reteplase (BM 06.022) in the Rat

1995 ◽  
Vol 74 (06) ◽  
pp. 1501-1510 ◽  
Author(s):  
J Kuiper ◽  
H van de Bilt ◽  
U Martin ◽  
Th J C van Berkel
Keyword(s):  
Plasminogen Activator ◽  
Liver Cells ◽  
The Novel ◽  
Uptake Mechanism ◽  
Liver Uptake ◽  
Lysosomal Compartment ◽  
Β Phase ◽  
Α Phase ◽  
Parenchymal Liver

SummaryThe catabolism of the novel plasminogen activator reteplase (BM 06.022) was described. For this purpose BM 06.022 was radiolabelled with l25I or with the accumulating label l25I-tyramine cellobiose (l25I-TC).BM 06.022 was injected at a pharmacological dose of 380 μg/kg b.w. and it was cleared from the plasma in a biphasic manner with a half-life of about 1 min in the α-phase and t1/2of 20-28 min in the β-phase. 28% and 72% of the injected dose was cleared in the α-phase and β-phase, respectively. Initially liver, kidneys, skin, bones, lungs, spleen, and muscles contributed mainly to the plasma clearance. Only liver and the kidneys, however, were responsible for the uptake and subsequent degradation of BM 06.022 and contributed for 75% to the catabolism of BM 06.022. BM 06.022 was degraded in the lysosomal compartment of both organs. Parenchymal liver cells were responsible for 70% of the liver uptake of BM 06.022. BM 06.022 associated rapidly to isolated rat parenchymal liver cells and was subsequently degraded in the lysosomal compartment of these cells. BM 06.022 bound with low-affinity to the parenchymal liver cells (550 nM) and the binding of BM 06.022 could be displaced by t-PA (IC50 5.6 nM), indicating that the low-density lipoprotein receptor-related protein (LRP) could be involved in the binding of BM 06.022. GST-RAP, which is an inhibitor of LRP, could in vivo significantly inhibit the uptake of BM 06.022 in the liver.It is concluded that BM 06.022 is metabolized primarily in the liver and the kidneys. These organs take up and degrade BM 06.022 in the lysosomes. The uptake mechanism of BM 06.022 in the kidneys is unknown, while LRP is responsible for a low-affinity binding and uptake of BM 06.022 in parenchymal liver cells.

Hepatoprotective Effects of Hovenia dulcis Fruit on Ethanol-Induced Liver Damage in vitro and in vivo

2009 ◽  
Vol 38 (2) ◽  
pp. 154-159 ◽  
Author(s):  
Yang-Hee You ◽  
Kuk-Yung Jung ◽  
Yoo-Hyun Lee ◽  
Woo-Jin Jun ◽  
Boo-Yong Lee
Keyword(s):  
Liver Damage ◽  
Hepatoprotective Effects ◽  
Hovenia Dulcis

EFECTOS CITOGENÉTICOS DE LA FÓRMULA COMERCIAL DE UNA TABLETA ANTIGRIPAL EN LOS ERITROCITOS DE SANGRE PERIFÉRICA DE RATÓN ÁRABE (MUS MUSCULUS LINNAEUS, 1758)

2013 ◽  
Vol 6 ◽  
Author(s):  
Saúl Flores Maya ◽  
Héctor Barrera Escorcia ◽  
Alexis Frausto Cornejo ◽  
Daniela Elizabeth Chávez Vázquez ◽  
Ana Cristina Hernández Cruz ◽  
...  
Keyword(s):  
Mus Musculus

La mezcla de analgésico, antipirético y antihistamínico de una pastilla antigripal de marca conocida fue evaluada en su capacidad de provocar daño cromosómico y citotoxicidad en sangre periférica de ratón árabe. El daño cromosómico fue evaluado utilizando la prueba de micronúcleos in vivo. El antigripal fue administrado a ratones de la línea árabe por vía oral en una dosis de 10.6 mg/Kg de peso en el curso de ocho h por tres días en un tratamiento agudo. Los cálculos del índice de toxicidad no fueron significativos estadísticamente entre los datos del grupo control negativo y los tratamientos con el antigripal. En cambio, este antigripal mostró efectos genotóxicos significativos a las 24, 48 y 72 h después de su aplicación. Este efecto puede ser causa de los componentes químicos del antigripal como son el paracetamol y la cafeína. En conclusión, la mezcla de analgésico, antipirético y antihistamínico de una pastilla antigripal no provoca daño celular pero muestra daño clastogénico durante el tratamiento agudo del antigripal en ratones de la línea árabe. El ratón Árabe mostró sensibilidad a los efectos de los agentes genotóxicos y, por tanto, este organismo debería ser incluido para estudios de genotoxicidad.

scholarly journals The Structure–Properties–Cytotoxicity Interplay: A Crucial Pathway to Determining Graphene Oxide Biocompatibility

2021 ◽  
Vol 22 (10) ◽  
pp. 5401
Author(s):  
Marta Dziewięcka ◽  
Mirosława Pawlyta ◽  
Łukasz Majchrzycki ◽  
Katarzyna Balin ◽  
Sylwia Barteczko ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Defense Mechanisms ◽  
Physicochemical Analysis ◽  
Experimental Models ◽  
Acheta Domesticus ◽  
Synthesis Process ◽  
Synthesis Methods ◽  
Toxicity Potential ◽  
Potential Applications

Interest in graphene oxide nature and potential applications (especially nanocarriers) has resulted in numerous studies, but the results do not lead to clear conclusions. In this paper, graphene oxide is obtained by multiple synthesis methods and generally characterized. The mechanism of GO interaction with the organism is hard to summarize due to its high chemical activity and variability during the synthesis process and in biological buffers’ environments. When assessing the biocompatibility of GO, it is necessary to take into account many factors derived from nanoparticles (structure, morphology, chemical composition) and the organism (species, defense mechanisms, adaptation). This research aims to determine and compare the in vivo toxicity potential of GO samples from various manufacturers. Each GO sample is analyzed in two concentrations and applied with food. The physiological reactions of an easy model Acheta domesticus (cell viability, apoptosis, oxidative defense, DNA damage) during ten-day lasting exposure were observed. This study emphasizes the variability of the GO nature and complements the biocompatibility aspect, especially in the context of various GO-based experimental models. Changes in the cell biomarkers are discussed in light of detailed physicochemical analysis.

Enhanced reproductive toxicities induced by phthalates contaminated microplastics in male mice (Mus musculus)

2021 ◽  
Vol 406 ◽  
pp. 124644
Author(s):  
Yongfeng Deng ◽  
Zehua Yan ◽  
Ruqin Shen ◽  
Yichao Huang ◽  
Hongqiang Ren ◽  
...  
Keyword(s):  
Mus Musculus ◽  
Male Mice
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