BackgroundThe phylogeny of tribe Brassiceae (Brassicaceae) has not yet been resolved because of its complex evolutionary history. This tribe comprises economically relevant species, including the genusMoricandiaDC. This genus is currently distributed in North Africa, Middle East, Central Asia and Southern Europe, where it is associated with arid and semi-arid environments. Although some species ofMoricandiahave been used in several phylogenetic studies, the phylogeny of this genus is not well established.MethodsHere we present a phylogenetic analysis of the genusMoricandiausing a nuclear (the internal transcribed spacers of the ribosomal DNA) and two plastidial regions (parts of the NADH dehydrogenase subunit F gene and thetrnT-trnF region). We also included in the analyses members of their sister genusRytidocarpusand from the close genusEruca.ResultsThe phylogenetic analyses showed a clear and robust phylogeny of the genusMoricandia. The Bayesian inference tree was concordant with the maximum likelihood and timing trees, with the plastidial and nuclear trees showing only minor discrepancies. The genusMoricandiaappears to be formed by two main lineages: the Iberian clade including three species, and the African clade including the four species inhabiting the Southern Mediterranean regions plusM. arvensis.DiscussionWe dated the main evolutionary events of this genus, showing that the origin of the Iberian clade probably occurred after a range expansion during the Messinian period, between 7.25 and 5.33 Ma. In that period, an extensive African-Iberian floral and faunal interchange occurred due to the existence of land bridges between Africa and Europa in what is, at present-days, the Strait of Gibraltar. We have demonstrated that a Spanish population previously ascribed toRytidocarpus moricandioidesis indeed aMoricandiaspecies, and we propose to name it asM. rytidocarpoidessp. nov. In addition, in all the phylogenetic analyses,M. foleyiappeared outside theMoricandialineage but within the genusEruca. Therefore,M. foleyishould be excluded from the genusMoricandiaand be ascribed, at least provisionally, to the genusEruca.