scholarly journals Transcript abundances of LIM transcription factor, 4CL, CAld5H and CesAs affect wood properties in Eucalyptus globulus

2011 ◽  
Vol 60 (1-6) ◽  
pp. 288-296 ◽  
Author(s):  
N. Negishi ◽  
K. Nanto ◽  
K. Hayashi ◽  
S. Onogi ◽  
A. Kawaoka
Keyword(s):  
Transcription Factor ◽  
Eucalyptus Globulus ◽  
Woody Plant ◽  
Wood Quality ◽  
Wood Properties ◽  
Gene Transcript ◽  
Lim Domain ◽  
Positive Correlation ◽  
Hardwood Species ◽  
Coa Ligase

AbstractEucalyptus globulusis the main hardwood species grown in pulpwood plantations in temperate regions of the world. We have cloned six genes influencing wood quality including the LIM domain transcription factor (LIM), 4-coumarate-CoA ligase (4CL), coniferaldehyde 5-hydroxylase (CAld5H) and the three catalytic units of cellulose synthase (CesA), fromE. globulus. The transcript abundances of LIM in basal stems of ten independentE. globuluslines showed similar patterns to those of 4CL. We investigated the correlation between gene transcript abundances and wood qualities such as Klason lignin (KL) content, syringaldehyde/vanillin (S/V) ratio and holocellulose (HC) content. Expression of the LIM and 4CL were positively correlated with KL content. A highly significant positive correlation was observed between CAld5H expression and S/V ratio. Furthermore, a ratio of the sum of the transcript abundances of three CesA1, CesA2 and CesA3 to 4CL showed a positive correlation with a ratio of HC/KL content that positively correlated with the chemically extracted fiber content in this woody plant.

scholarly journals Reduction of Lignin Content by Suppression of Expression of the LIM Domain Transcription Factor in Eucalyptus camaldulensis

2006 ◽  
Vol 55 (1-6) ◽  
pp. 269-277 ◽  
Author(s):  
A. Kawaoka ◽  
K. Nanto ◽  
K. Ishii ◽  
H. Ebinuma
Keyword(s):  
Transcription Factor ◽  
Lignin Content ◽  
Lignin Biosynthesis ◽  
Eucalyptus Camaldulensis ◽  
Transcript Level ◽  
Amino Acid Identity ◽  
Lim Domain ◽  
Cis Acting ◽  
Coa Ligase ◽  
Phenylalanine Ammonialyase

Abstract We report a reduction of lignin content in the woody plant Eucalyptus camaldulensis by the suppression of gene expression of the LIM domain transcription factor. Previously, we identified a cDNA encoding the tobacco (Nicotiana tabacum) LIM domain transcription factor, Nt1iml, involved in lignin biosynthesis and that specifically binds to an important cis-acting element, the PALbox sequence. The orthologous Eucalyptus gene of Ntliml, namely Ecliml, was isolated from the E. camaldulensis cDNA library (84% amino acid identity). The antisense Ntliml construct with a kanamycin-resistant gene was introduced into E. camaldulensis. The transgenic Eucalyptus plants grown in the greenhouse showed decreased expression levels of severallignin biosynthesis genes, phenylalanine ammonialyase (PAL), cinnamate-4-hydroxylase (C4H) and 4-hydroxycinnamate CoA ligase (4CL). The abnormal phenotypic changes and a 29% reduction of lignin content were observed in the line LG12, in which the transcript level of Ecliml was most1y suppressed. Ec1iml is one of the key transcription factors involved in lignin biosynthesis.

Genetic relationships between wood quality traits and diameter growth of juvenile core wood in Sitka spruce

2013 ◽  
Vol 43 (1) ◽  
pp. 1-6 ◽  
Author(s):  
S.G. Kennedy ◽  
A.D. Cameron ◽  
S.J. Lee
Keyword(s):  
Growth Rate ◽  
Genetic Correlation ◽  
Modulus Of Elasticity ◽  
Genetic Relationships ◽  
Wood Quality ◽  
Wood Properties ◽  
Sitka Spruce ◽  
Acoustic Velocity ◽  
Picea Sitchensis ◽  
Good Growth

The trend towards shorter rotations in planted conifer stands has resulted in a reduction in the proportion of mature wood relative to juvenile core wood, raising concerns that the mechanical performance of sawn battens will be affected. The potential to improve the wood quality of the juvenile core of Sitka spruce (Picea sitchensis (Bong.) Carrière) without compromising growth rate was investigated. Rapid and inexpensive indirect methods of assessing wood properties on standing trees using a Pilodyn gun and acoustic velocity were compared with direct measurements made on wood samples cut from the juvenile core. Strong genetic correlations were observed between Pilodyn gun values and direct measures of density (–0.76) and between the square of acoustic velocity and modulus of elasticity (0.73). The genetic correlation between the square of acoustic velocity and microfibril angle was also strong (–0.84). These results suggest that indirect assessments of wood properties within juvenile core wood are sufficiently reliable for these techniques to be used in the Sitka spruce breeding programme. Although a strong negative genetic correlation between diameter at breast height and density was noted (–0.79), sufficient variation exists within the breeding population to select families with both good growth rate and high modulus of elasticity wood.

scholarly journals Relationship between Growth and Wood properties in Agathis sp. Planted in Indonesia

2017 ◽  
Vol 3 (1) ◽  
pp. 1-5
Author(s):  
Futoshi Ishiguri ◽  
Kazuko Makino ◽  
Imam Wahyudi ◽  
Jun Tanabe ◽  
Yuya Takashima ◽  
...  
Keyword(s):  
Wood Quality ◽  
Basic Density ◽  
Tree Breeding ◽  
Wood Properties ◽  
Stem Diameter ◽  
Breeding Programs ◽  
Significant Difference ◽  
Pilodyn Penetration ◽  
The Mean ◽  
The Relationship

The present study clarified the relationship between the growth and wood properties of 54-year-old Agathis sp. trees planted in Indonesia. Stem diameter, pilodyn penetration, and stress-wave velocity (SWV) were measured for all trees (35 trees) in a plot (30  30 m) located almost at the center of a stand. Based on the mean stem diameter, 10 standard trees in a plot were selected for measuring the basic density (BD) and compressive strength parallel to grain (CS). Core samples (5 mm in diameter) were collected from the 10 selected trees to determine BD and CS. The mean stem diameter, pilodyn penetration and SWV in the plot were 40.2  11.3 cm, 23.4  2.1 cm, and 3.85  0.43 km/s, respectively. No  significant correlation coefficeint (r = -0.327, no significance at 5% level) was obtained between stem diameter and SWV. The mean BD and CS in the 10 trees were 0.42  0.03 g/cm3  and 28.1  2.7 MPa, respectively. A significant positive  correlation was observed between BD and CS. Analysis of variance (ANOVA) revealed a significant difference between BD and CS values of the 10 trees, indicating that wood properties may differ among trees with the same standard growth in a stand. From these results, we concluded that wood quality improvement in this species could be achieved by selecting  trees with high density and strength in tree breeding programs.

Effect of fertilizer on wood properties of Eucalyptus globulus

2000 ◽  
Vol 30 (1) ◽  
pp. 136-144 ◽  
Author(s):  
C A Raymond ◽  
A Muneri
Keyword(s):  
Eucalyptus Globulus ◽  
Fibre Length ◽  
Basic Density ◽  
Wood Properties ◽  
Pulp Yield ◽  
Growth Responses ◽  
P Concentration ◽  
Tree Growth Rate ◽  
Western Australian ◽  
P Fertilizers

The effects of N and P fertilizers applied to Eucalyptus globulus Labill. at plantation establishment on basic density, fibre length, fibre coarseness, predicted pulp yield, and N and P concentration in the wood were examined by sampling four fertilizer factorial trials: three in Victoria and one in Western Australia. Treatments sampled were control, maximum levels of N and P by themselves and combined. Growth responses varied across sites with significant growth responses at the Victorian sites but no response at the Western Australian site. An interaction was suggested between rainfall and the effects of the fertilizer; wood properties at the drier sites were detrimentally affected by fertilizer but there was little effect at the wetter sites. On the two drier sites, application of both N and P, alone or in combination, resulted in changes in density, shorter fibres, and slightly lower predicted pulp yield. Addition of both N and P increased the levels of these nutrients in the wood at the three Victorian sites. Changes occurred in wood properties in the absence of growth responses to the applied fertilizer indicating that these changes were not induced by changing tree growth rate.

Technological Properties of Wood from Trees in Polluted Regions

IAWA Journal ◽  
1986 ◽  
Vol 7 (4) ◽  
pp. 389-397 ◽  
Author(s):  
A. Frühwald
Keyword(s):  
Moisture Content ◽  
Wood Quality ◽  
Wood Properties ◽  
Biological Indicators ◽  
Strength Properties ◽  
West Germany ◽  
Technological Properties ◽  
Growth Rings ◽  
Physical Chemical ◽  
Faster Development

Wood quality from about 230 healthy and diseased fir, spruce, pine and beech trees from various locations in West Germany was tested. In general results are satisfactory for mechanical wood properties, but some physical, chemical and biological indicators lead to the conclusion that logs from heavily diseased trees may be attacked a !ittle earlier and faster by microorganisms during storage. Width of growth rings is reduced in severely damaged softwood trees on most locations whereas beech shows no significant reduction. Declining health of the trees had no or little influence on wood density; the modulus of elasticity and strength were at normal levels and independent of tree damage. Width and moisture content of sapwood were reduced little in pines but more in spruce and fir. A tendency to a more intensely developed irregular brown heartwood in severeIy damaged beech trees could not be proved until now. Storage of spruce in a log yard for four months has shown a slightly faster development of discoloration caused by fungi in diseased trees whereas after seven months wood from healthy trees was more discoloured. After storage strength properties are not or only slightly reduced, but no difference was found between trees assigned to the various health classes. After two years of storage under sprinkling water, pine logs from healthy and diseased trees show no difference in discoloration.

Grade yields and wood properties of Norway spruce [Picea abies (L.) Karst.] from the Maritimes

1995 ◽  
Vol 71 (4) ◽  
pp. 473-478
Author(s):  
Y. H. Chui
Keyword(s):  
Mechanical Properties ◽  
Picea Abies ◽  
Norway Spruce ◽  
Wood Quality ◽  
Wood Properties ◽  
Bending Properties ◽  
Clear Wood ◽  
Comparative Information ◽  
Grade Yield

Norway spruce [Picea abies (L.) Karst.] is one of the major non-native softwood species in the Maritimes. A project was undertaken to evaluate the grade yields and mechanical properties of Norway spruce. The project also provided comparative information on the wood quality of two Norway spruce provenances from Germany and Poland. Four plantations were selected for the study with two of these plantations containing trees of known provenances. One plantation was mature and the other three were juvenile. In total, 530 pieces of lumber and the same number of matched small clear specimens were tested for bending properties. Prior to testing, the lumber was visually graded according to both British and Canadian specifications. Quality of lumber varied significantly between sites. Lumber from the Polish provenance had slightly better mechanical properties than that from trees of the German provenance. Compared with published information, the plantation-grown Norway spruce had lower clear wood bending properties and specific gravity than primary eastern Canadian spruce species and balsam fir, and natural Norway spruce grown in Europe. Key words: Norway spruce [Picea abies (L.) Karst.], wood quality, bending properties, grade yield

Genotypic variation in the basic density, dynamic modulus of elasticity and tracheid traits of Pinus elliottii in three progeny trials in southern China

Holzforschung ◽  
2019 ◽  
Vol 74 (1) ◽  
pp. 1-9
Author(s):  
Meng Lai ◽  
Leiming Dong ◽  
Chunhui Leng ◽  
Lu Zhang ◽  
Min Yi
Keyword(s):  
Genetic Correlation ◽  
Modulus Of Elasticity ◽  
Dynamic Modulus ◽  
Southern China ◽  
Wood Quality ◽  
Basic Density ◽  
Pinus Elliottii ◽  
Wood Properties ◽  
Correlated Response ◽  
Dynamic Modulus Of Elasticity

AbstractA firm understanding of the genetic relationships among wood properties is a prerequisite for breeding for higher wood quality in Pinus elliottii families. To examine and deal with such relationships, increment cores were sampled at breast height from 1260 trees in 42 open-pollinated families in three 27-year-old Slash pine progeny trials in southern China, and genetic variation, genotype-by-environment (G × E) interaction, genetic correlation and correlated response were investigated. The basic density (BD), dynamic modulus of elasticity (MOED) and tracheid traits were found to be under moderate to strong genetic control for the three locations combined, with individual narrow-sense and family mean heritability ranging from 0.28 to 0.44 and 0.52 to 0.69, respectively. Type B genetic correlation estimates indicated that the G × E interaction had a small-level influence on wood properties. Strong genetic correlations (rg) were found between BD and MOED at the three sites (rg = 0.46–0.85), and BD or MOED showed moderate to strong correlations with most tracheid traits at specific localities. In tree breeding programs, one possible strategy would be to improve pulpwood quality and the strength of structural wood through selection for different wood quality traits.

Direct Effects of Wood Characteristics on Pulp and Handsheet Properties of Eucalyptus globulus

Holzforschung ◽  
2002 ◽  
Vol 56 (3) ◽  
pp. 244-252 ◽  
Author(s):  
R. Wimmer ◽  
G.M. Downes ◽  
R. Evans ◽  
G. Rasmussen ◽  
J. French
Keyword(s):  
Wood Density ◽  
Eucalyptus Globulus ◽  
Strong Predictor ◽  
Fibre Length ◽  
Microfibril Angle ◽  
Wood Properties ◽  
Bending Stiffness ◽  
Pulp Yield ◽  
Paper Properties ◽  
Handsheet Properties

Summary Fibre length, microfibril angle and wood density were measured in eight-year-old Eucalyptus globulus clones planted on three different sites. Samples were chipped and pulped, and the pulps beaten prior to preparation of 60g/m2 handsheets. Eleven physical handsheet properties, together with pulp freeness, were measured using standard methods. Direct relationships between wood properties and pulp/paper properties were studied using path analysis. Fibre length had a strong, direct effect on tear index, bending stiffness, freeness, pulp yield and active alkali consumption. Wood density was a strong predictor of most handsheet properties: high density woods produced bulkier, more porous sheets with higher bending stiffness, while lower density woods produced smoother, denser sheets with higher tensile strength.The effect of microfibril angle was small and more evident in handsheets made from beaten pulp.

The Four and a Half LIM Domain Protein 2 (FHL2) Interacts with CALM and Is Highly Expressed in AML with Complex Aberrant Karyotpes.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4337-4337 ◽  
Author(s):  
Zlatana Pasalic ◽  
Belay Tizazu ◽  
Leticia Archangelo ◽  
Alexandre Krause ◽  
Greif Philipp ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Fusion Protein ◽  
Myeloid Leukemia ◽  
Fusion Gene ◽  
Luciferase Reporter ◽  
Lim Domain ◽  
Yeast Two Hybrid ◽  
Lim Domain Protein ◽  
Domain Protein ◽  
Two Hybrid

Abstract The balanced chromosomal translocation t(10;11)(p13;q14) results in the CALM/AF10 fusion gene. This translocation is found in acute myeloid leukemia (AML), T-cell acute lymphoblastic leukaemia (T-ALL) and malignant lymphoma. The CALM/AF10 fusion gene has recently been shown to cause an aggressive biphenotypic leukemia in a murine bone marrow transplant model. The CALM (Clathrin Assembly Lymphoid Myeloid leukemia gene) gene product is a clathrin assembly protein which plays a role in clathrin mediated endocytosis and trans Golgi network trafficking. AF10 is a putative transcription factor most likely involved in processes related to chromatin organization and has polycomb group gene like properties. To learn more about the function of the CALM/AF10 fusion protein, we searched for protein interaction partners of CALM. In a yeast two hybrid screen the four and a half LIM domain protein FHL2 was identified as putative CALM interacting partner. The CALM FHL2 interaction was confirmed by co-transformation assay in yeast and by GST-pulldown experiments. The FHL2 interaction domain of CALM was mapped to amino acids 294 to 335 of CALM using the yeast two hybrid assay. In co-localization studies with transiently expressed fluorescent protein tagged CALM and FHL2, both proteins showed cytoplasmatic localization. Expression analysis (Affymetrix based) in different AML subtypes showed a significantly higher expression of FHL2 in AML with complex aberrant karyotypes compared to AML with normal karyotypes or balanced chromosomal translocations like the t(8;21), inv(16) or t(15;17). FHL2, which is also known as DRAL (downregulated in rhabdomyosarcoma LIM protein), is a TP53 responsive gene known to interact with numerous proteins in both the nucleus and the cytoplasm and can function as a transcriptional cofactor. Known FHL2 interactors include TP53, BRCA1, PLZF (promyelocytic leukemia zinc finger protein), the proto-oncogene SKI1 and beta-catenin. High expression of FHL2 in breast cancer has recently been shown to be associated with an adverse prognosis. CALM has been shown to shuttle between the nucleus and the cytoplasm because inhibition of CREM-mediated nuclear export by leptomycin B leads to the accumulation of CALM in the nucleus. Reporter gene assays using a GAL4-DNA binding domain CALM fusion protein and a GAL4 responsive luciferase reporter were able to demonstrate a transcriptional activation function of CALM. We are currently investigation the effect of FHL2 co-expression on this aspect of the CALM function. It is thus conceivable that FHL2 is playing an important role in CALM/AF10-mediated leukemogenesis by tethering the CALM/AF10 fusion protein to various nuclear transcription factor complexes.

Sign in / Sign up

Export Citation Format

Share Document