Chemical constituents and antiproliferative effects of cultured Mougeotia nummuloides and Spirulina major against cancerous cell lines

AbstractIn this study, the effect ofMougeotia nummuloidesandSpirulina majoron Vero cells (African green monkey kidney), C6 cells (rat brain tumor cells) and HeLa cells (human uterus carcinoma) was investigatedin vitro. The antiproliferative effect of the methanol extract ofM. nummuloidesandS. majorcompared with 5-fluorourasil (5-FU) and cisplatin was tested at various concentrations using the BrdU Cell Proliferation ELISA. BothM. nummuloidesandS. majorextracts significantly inhibited the proliferation of Vero, HeLa and C6 cancer cell lines with IC50and IC75values. TheM. nummuloidesextract exhibited higher activity than 5-FU and cisplatin on Vero and C6 cells at high concentrations. TheS. majorextract revealed better antifproliferative activity than standards against Vero cells at 500 μg/mL. The compounds of methanol extracts were determined by GC-MS after the silylation process. Trehalose, monostearin and 1-monopalmitin were detected as major products in theM. nummuloidesextract where as in theS. majorextract; monostearin, 1-monopalmitin and hexyl alcohol were the main constituents.

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Bioactive Volatile Content of the Stem and Root ofCentaurea carduiformisDC. subsp.carduiformisvar.carduiformis

Journal of Chemistry ◽

10.1155/2013/125286 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 10

Author(s):

Ibrahim Demirtas ◽

Ayse Sahin

Keyword(s):

Cell Lines ◽

Vero Cells ◽

Hexane Extract ◽

African Green Monkey Kidney ◽

Brain Tumor Cells ◽

Green Monkey ◽

Rat Brain Tumor ◽

Medicinal Properties ◽

Asteraceae Family

Several species ofCentaurea, biennial (or more rarely perennial) plants which belong to the Asteraceae family, possess medicinal properties and are currently used in phytotherapy. In the present study, antiproliferative activity of hexane extract from stems and roots ofCentaurea carduiformisDC. subsp.carduiformisvar.carduiformis(CCS and CCR) on Vero cells (African green monkey kidney), C6 cells (Rat Brain tumor cells), and HeLa cells (human uterus carcinoma) was investigatedin vitro. Antiproliferative effect of the extract was tested at 500 μg/mL and 1000 μg/mL using BrDu Cell Proliferation ELISA. The hexane extract was significantly inhibited proliferation of Vero, HeLa, and C6 cancer cell lines with absorbance values. The extract of CCS and CCR showed the highest activity against the Vero, HeLa, and C6 cell lines at 500 μg/mL and 1000 μg/mL.

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Chemical Constituents of the New Endophytic Fungus Mycosphaerella sp. nov. and Their Anti-parasitic Activity

Natural Product Communications ◽

10.1177/1934578x1100600620 ◽

2011 ◽

Vol 6 (6) ◽

pp. 1934578X1100600 ◽

Cited By ~ 4

Author(s):

Eufemio Moreno ◽

Titto Varughese ◽

Carmenza Spadafora ◽

A. Elizabeth Arnold ◽

Phyllis D. Coley ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Endophytic Fungus ◽

Leishmania Donovani ◽

Chemical Constituents ◽

Vero Cells ◽

Cancer Cell Lines ◽

Antiparasitic Activity ◽

Minor Compounds

Chemical investigation of a new endophytic fungus, Mycosphaerella sp. nov. strain F2140, associated with the foliage of the plant Psychotria horizontalis (Rubiaceae) in Panama, resulted in the isolation of cercosporin (1) and a new cercosporin analog (3) as the major components. The structures of minor compounds in the extract were elucidated by detailed spectroscopic analysis as 2-(2-butyl)-6-ethyl-3-hydroxy-6-methylcyclohex-2-ene-1, 5-dione (4), 3-(2-butyl)-6-ethyl-5-hydroxy-2-methoxy-6-methyl-cyclohex-2-enone (5), and an isomer of 5 (6). To study the influence of the hydroxy groups on the antiparasitic activity of cercosporin, compound 1 was acetylated to obtain derivative 2. The isolated compounds 1-6 were tested in vitro to determine their antiparasitic activity against the causal agents of malaria ( Plasmodium falciparum), leishmaniasis ( Leishmania donovani), and Chagas disease ( Trypanosoma cruzi). Cytotoxicity and potential anticancer activity of these compounds were evaluated using mammalian Vero cells and MCF7 cancer cell lines, respectively. Compounds 1 and 2 displayed high potency against L. donovani (IC50 0.46 and 0.64 μM), T. cruzi (IC50 1.08 and 0.78 μM), P. falciparum (IC50 1.03 and 2.99 μM), and MCF7 cancer cell lines (IC50 4.68 and 3.56 μM). Compounds 3-6 were not active in these assays at a concentration of 10 μg/mL.

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Development of Eimeria bovis in vitro: suitability of several bovine, human and porcine endothelial cell lines, bovine fetal gastrointestinal, Madin–Darby bovine kidney (MDBK) and African green monkey kidney (VERO) cells

Parasitology Research ◽

10.1007/s00436-001-0531-1 ◽

2001 ◽

Vol 88 (4) ◽

pp. 301-307 ◽

Cited By ~ 54

Author(s):

C. Hermosilla ◽

B. Barbisch ◽

A. Heise ◽

S. Kowalik ◽

H. Zahner

Keyword(s):

Endothelial Cell ◽

Cell Lines ◽

Vero Cells ◽

African Green Monkey ◽

Monkey Kidney ◽

African Green Monkey Kidney ◽

Bovine Kidney ◽

Green Monkey ◽

Porcine Endothelial Cell

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In Vitro Evaluation of Chitosan Induced Cytotoxicity against Cancerous Cell lines: MCF-7, Saos-2 and HeLa

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520619666190507113949 ◽

2019 ◽

Vol 19 ◽

Author(s):

Zeinab Abedian ◽

Niloofar Jenabian ◽

Ali Akbar Moghadamnia ◽

Ebrahim Zabihi ◽

Roghayeh Pourbagher ◽

...

Keyword(s):

Flow Cytometry ◽

Cell Lines ◽

Anticancer Agents ◽

Fibroblast Cells ◽

Apoptosis Induction ◽

Cytotoxic Effects ◽

Cancerous Cell ◽

Significant Concentration ◽

Mcf 7

Objective/ Background: Cancer is still the most common cause of morbidity in world and new powerful anticancer agents without severe side effects from natural sources is important. Methods: The evaluation of cytotoxicity and apoptosis induction was carried out in MCF-7,HeLa and Saos-2 as cancerous cell lines with different histological origin and human fibroblast served as control normal cell. The cells were treated with different concentrations of chitosan and the cytotoxicity was determined using MTT assay after 24, 48 and 72 h .The mode of death was evaluated by flow cytometry . Results: While both types of chitosan showed significant concentration-dependently cytotoxic effects against the three cancerous cell lines, fibroblast cells showed somehow more compatibility with chitosan. On the other hand, there were no significant differences between LMWC and HMWC cytotoxicity in all cell lines. The flow cytometry results showed the apoptosis pattern of death more in Saos-2 and HeLa while necrosis was more observable with MCF7. Also higher viability with both types of chitosan was seen in fibroblast as normal cells Conclusion: Chitosan shows anticancerous effect against 3 cancerous cell lines, while it is compatible with normal diploid fibroblast cells. Furthermore, it seems that the molecular weight of chitosan does not affect its anticancerous property.

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Low-pH Endocytic Entry of the Porcine Alphaherpesvirus Pseudorabies Virus

Journal of Virology ◽

10.1128/jvi.01849-18 ◽

2018 ◽

Vol 93 (2) ◽

Cited By ~ 6

Author(s):

Jonathan L. Miller ◽

Darin J. Weed ◽

Becky H. Lee ◽

Suzanne M. Pritchard ◽

Anthony V. Nicola

Keyword(s):

Cell Lines ◽

Pseudorabies Virus ◽

Vero Cells ◽

Low Ph ◽

Host Cells ◽

Multiple Model ◽

Trade Restrictions ◽

African Green Monkey Kidney ◽

Entry Pathway ◽

Model Cell

ABSTRACTThe alphaherpesvirus pseudorabies virus (PRV) is the causative agent of pseudorabies, a disease of great economic and welfare importance in swine. Other alphaherpesviruses, including herpes simplex virus (HSV), utilize low-pH-mediated endocytosis to enter a subset of cell types. We investigated whether PRV used this entry pathway in multiple laboratory model cell lines. Inhibition of receptor-mediated endocytosis by treatment with hypertonic medium prevented PRV entry. PRV entry into several cell lines, including porcine kidney (PK15) cells and African green monkey kidney (Vero) cells, was inhibited by noncytotoxic concentrations of the lysosomotropic agents ammonium chloride and monensin, which block the acidification of endosomes. Inactivation of virions by acid pretreatment is a hallmark of viruses that utilize a low-pH-mediated entry pathway. Exposure of PRV virions to pH 5.0 in the absence of host cell membranes reduced entry into PK15 and Vero cells by >80%. Together, these findings suggest that endocytosis followed by fusion with host membranes triggered by low endosomal pH is an important route of entry for PRV.IMPORTANCEPRV is a pathogen of great economic and animal welfare importance in many parts of the world. PRV causes neurological, respiratory, and reproductive disorders, often resulting in mortality of young and immunocompromised animals. Mortality, decreased production, and trade restrictions result in significant financial losses for the agricultural industry. Understanding the molecular mechanisms utilized by PRV to enter host cells is an important step in identifying novel strategies to prevent infection and spread. A thorough understanding of these mechanisms will contribute to a broader understanding of alphaherpesvirus entry. Here, we demonstrate PRV entry into multiple model cell lines via a low-pH endocytosis pathway. Together, these results provide a framework for elucidating the early events of the PRV replicative cycle.

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Chemical Constituents from Walsura pinnata (Meliaceae)

Natural Product Communications ◽

10.1177/1934578x1701200905 ◽

2017 ◽

Vol 12 (9) ◽

pp. 1934578X1701200

Author(s):

Mohamad A. Mahdzir ◽

Jamil A. Shilpi ◽

Norfaizah Mahmud ◽

Sujatha Ramasamy ◽

Khalijah Awang

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Human Cancer ◽

Chemical Constituents ◽

Cancer Cell Lines ◽

Phytochemical Study ◽

Triterpene Acid ◽

Human Cancer Cell Lines ◽

Esi Ms

A phytochemical study on the bark of Walsura pinnata has led to the isolation of a new oleanane triterpene acid, 3-oxo-olean-9(11),12-dien-28-oic acid (1), together with nine known compounds (2–10). Their structures were established on the basis of the detailed spectroscopic analysis, including one- and two-dimensional NMR, ESI-MS and HR-ESI-MS techniques. Compounds 2, 3, 5, 6 and 8 were isolated from W. pinnata for the first time. Compounds 3 and 4 showed in vitro growth inhibitory activity against two human cancer cell lines MCF-7 and SK-OV-3 with IC50 values within the range of 8.85 - 18.28 μg/mL. To the best of our knowledge, this is the first report on the cytotoxic activity of compound 3 towards both cancer cell lines.

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Cancer Alters the Metabolic Fingerprint of Extracellular Vesicles

Cancers ◽

10.3390/cancers12113292 ◽

2020 ◽

Vol 12 (11) ◽

pp. 3292

Author(s):

Mari Palviainen ◽

Kirsi Laukkanen ◽

Zeynep Tavukcuoglu ◽

Vidya Velagapudi ◽

Olli Kärkkäinen ◽

...

Keyword(s):

Cell Lines ◽

Cancer Cell ◽

Extracellular Vesicles ◽

Western Blotting ◽

Cell Metabolism ◽

Cancer Cell Lines ◽

Cancerous Cell ◽

Metabolic Fingerprint ◽

And Control

Cancer alters cell metabolism. How these changes are manifested in the metabolite cargo of cancer-derived extracellular vesicles (EVs) remains poorly understood. To explore these changes, EVs from prostate, cutaneous T-cell lymphoma (CTCL), colon cancer cell lines, and control EVs from their noncancerous counterparts were isolated by differential ultracentrifugation and analyzed by nanoparticle tracking analysis (NTA), electron microscopy (EM), Western blotting, and liquid chromatography-mass spectrometry (LC-MS). Although minor differences between the cancerous and non-cancerous cell-derived EVs were observed by NTA and Western blotting, the largest differences were detected in their metabolite cargo. Compared to EVs from noncancerous cells, cancer EVs contained elevated levels of soluble metabolites, e.g., amino acids and B vitamins. Two metabolites, proline and succinate, were elevated in the EV samples of all three cancer types. In addition, folate and creatinine were elevated in the EVs from prostate and CTCL cancer cell lines. In conclusion, we present the first evidence in vitro that the altered metabolism of different cancer cells is reflected in common metabolite changes in their EVs. These results warrant further studies on the significance and usability of this metabolic fingerprint in cancer.

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Attachment of Salmonella to mammalian cells in vitro

Canadian Journal of Microbiology ◽

10.1139/m83-263 ◽

1983 ◽

Vol 29 (12) ◽

pp. 1731-1735 ◽

Cited By ~ 5

Author(s):

Clifford S. Mintz ◽

Dean O. Cliver ◽

R. H. Deibel

Keyword(s):

Cell Lines ◽

Mammalian Cells ◽

Culture Cell ◽

Intestinal Cell ◽

Tissue Culture Cell ◽

Bacterial Cells ◽

Intestinal Cell Line ◽

Hela Cell Lines ◽

High Concentrations

The attachment of Salmonella typhimurium strain PHL67342 to several mammalian tissue culture cell lines was investigated. Strain PHL67342 failed to attach in significant numbers to the Buffalo green monkey (BGM), swine testicular (ST), and HeLa cell lines. Significant attachment was observed with the Henle intestinal cell line. Log-phase cells of strain PHL67342 attached in greatest numbers to the Henle cells after 45 min of incubation at 37 °C. Attachment to the Henle cells was not affected by D-mannose or D-galactose, but was markedly inhibited by high concentrations of alpha-methyl-D-mannoside. Also, Salmonella lipopolysaccharide had no effect on the attachment of strain PHL67342 to the Henle cells. Fimbriae were not detected on the bacterial cells used in the adherence experiments. These results suggest that some bacterial factor(s) other than fimbriae and lipopolysaccharide mediate the attachment of strain PHL67342 to the Henle cells.

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Attachment and invasion ofToxoplasma gondiiandNeospora caninumto epithelial and fibroblast cell linesin vitro

Parasitology ◽

10.1017/s0031182005008310 ◽

2005 ◽

Vol 131 (5) ◽

pp. 583-590 ◽

Cited By ~ 13

Author(s):

YING LEI ◽

M. DAVEY ◽

J. T. ELLIS

Keyword(s):

Cell Lines ◽

Neospora Caninum ◽

Fibroblast Cell ◽

Cell Types ◽

Vero Cells ◽

Host Cells ◽

Trypsin Treatment ◽

Epithelial Cell Lines ◽

Pre Treatment

Attachment and invasion ofToxoplasma gondiiandNeospora caninumto a cat and a dog fibroblast cell line and 2 epithelial cell lines (a cat kidney and Vero) were comparedin vitrousing fluorescence antibody methodology. In addition, trypsin treatment of tachyzoites was used to determine whether protein molecules were essential to the process of invasion. The results show that bothT. gondiiandN. caninuminvaded all 4 cell lines, and that pre-treatment ofT. gondiitachyzoites with trypsin caused an increase in the ability of the parasite to invade these host cells. FurthermoreT. gondii, in comparison toN. caninum, invaded all 4 cell lines at greater levels. The results here support the conclusion that bothT. gondiiandN. caninumhave the ability to invade a variety of cell types including both dog and cat cells, and questions the utility of Vero cells as an appropriate host cell forin vitrostudies on the biology of these taxa.

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