STUDIES ON SULFATION FACTOR (SF) ACTIVITY OF HUMAN SERUM

1961 ◽  
Vol 36 (1) ◽  
pp. 31-50 ◽  
Author(s):  
Sven Almqvist
Keyword(s):  
Human Serum ◽  
Block Design ◽  
Human Growth ◽  
Normal Subjects ◽  
Randomized Block Design ◽  
S Uptake ◽  
Local Reference ◽  
Improved Design ◽  
One Year

ABSTRACT An improved design of the in vitro bioassay of SF activity in human serum, based on the 35S-uptake of cartilage from hypophysectomized immature rats, has been evaluated. The method is based on a completely randomized block design, each assay statistically evaluable. The elimination of the error among the rats improved the mean precision (λ)rom 0.28 to 0.14. L̄ωas 6.3. The suitability of the design was shown in a series of 157 consecutive assays, in which 10 % had indeterminate confidence limits when P [2266] 0.05. The standard error of 13 replicate assays within one group was ± 1.9%. Six duplicate assays of sera of different SF activities on two groups had a mean difference of 0.09 SF units per ml. The sensitivity of the method was high, [2266] 0.005 units of SF activity, and permitted its application to normal subjects as well as patients with hypopituitarism. The response of the animal strain was stable for at least one year. The limits of the linear range of the dose-response curve with the local reference serum were 35 and 140 μl of serum. The serum factor was stable at −20° C for one year and at +18°C for one week. Although human growth hormone and glutamine in vitro significantly increased the 35S-incorporation, each of them could account for only a small part of the SF activity of normal human serum.

scholarly journals THE GROWTH PERFORMANCE OF CITRUS DERIVED FROM SOMATIC EMBRYOGENESIS PLANTLET AND SCION STOCK

2014 ◽  
Vol 15 (2) ◽  
pp. 71
Author(s):  
Nirmala Friyanti Devy ◽  
Yenni Yenni ◽  
Hardiyanto Hardiyanto
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Performance ◽  
Block Design ◽  
Good Alternative ◽  
Research Station ◽  
Randomized Block Design ◽  
Citrus Rootstock ◽  
Old Japanese ◽  
One Year

Somatic embryogenesis (SE) of callus culture in vitro is one of citrus propagation ways for producing free virus and genetically true-to-type plantlets. To induce growing of plantlets derived from this technology, they should be grafted ex vitro onto a citrus rootstock. The research aimed to evaluate the growth performance of citrus plants cv. Siam Kintamani (Citrus nobilis L.) that used both plantlets and scions as their stocks. The research was conducted at Tlekung Research Station, Indonesian Citrus and Subtropical Fruit Research Institute from June 2011 to December 2012. The treatments were done at nursery house by grafting a plantlet and budding a scion onto an eight-month-old Japanese Citroon (JC) rootstock plant. The grafted and budded plants of one-year old were maintained at nursery house then transplanted into the field. In the field, the research was arranged in a randomized block design with three replications and used 15 plants as unit samples. The results showed that the vegetative growth of Siam Kintamani seedling derived from SE or grafted plant was faster than that of budded plant started from 10 to 12 months after treatment in the nersery house. In the field, the growth of SE grafted plant was only significantly different up to 6 months after transplanting. Plantlets produced from SE in vitro propagation can be used as a good alternative stock material for producing healthy citrus plants. Therefore, a further research is required especially on varieties used, reproductive growth and massive planlets production.

scholarly journals THE GROWTH PERFORMANCE OF CITRUS DERIVED FROM SOMATIC EMBRYOGENESIS PLANTLET AND SCION STOCK

2014 ◽  
Vol 15 (2) ◽  
pp. 71
Author(s):  
Nirmala Friyanti Devy ◽  
Yenni Yenni ◽  
Hardiyanto Hardiyanto
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Performance ◽  
Block Design ◽  
Good Alternative ◽  
Research Station ◽  
Randomized Block Design ◽  
Citrus Rootstock ◽  
Old Japanese ◽  
One Year

Somatic embryogenesis (SE) of callus culture in vitro is one of citrus propagation ways for producing free virus and genetically true-to-type plantlets. To induce growing of plantlets derived from this technology, they should be grafted ex vitro onto a citrus rootstock. The research aimed to evaluate the growth performance of citrus plants cv. Siam Kintamani (Citrus nobilis L.) that used both plantlets and scions as their stocks. The research was conducted at Tlekung Research Station, Indonesian Citrus and Subtropical Fruit Research Institute from June 2011 to December 2012. The treatments were done at nursery house by grafting a plantlet and budding a scion onto an eight-month-old Japanese Citroon (JC) rootstock plant. The grafted and budded plants of one-year old were maintained at nursery house then transplanted into the field. In the field, the research was arranged in a randomized block design with three replications and used 15 plants as unit samples. The results showed that the vegetative growth of Siam Kintamani seedling derived from SE or grafted plant was faster than that of budded plant started from 10 to 12 months after treatment in the nersery house. In the field, the growth of SE grafted plant was only significantly different up to 6 months after transplanting. Plantlets produced from SE in vitro propagation can be used as a good alternative stock material for producing healthy citrus plants. Therefore, a further research is required especially on varieties used, reproductive growth and massive planlets production.

Shooting and Rooting Behaviour in Relation to Season, Age and Growing Conditions of Dendrocalamus asper Backer

2020 ◽  
Vol 27 (1) ◽  
pp. 56-60
Author(s):  
Reena Joshi ◽  
Salil Tewari ◽  
Rajesh Kaushal
Keyword(s):  
Summer Monsoon ◽  
Block Design ◽  
Research Centre ◽  
Agroforestry Research ◽  
Randomized Block Design ◽  
Different Seasons ◽  
Dendrocalamus Asper ◽  
Growing Conditions ◽  
One Year ◽  
Effect Of Age

The experiment was conducted at Agroforestry Research Centre, of G. B. Pant University of Agriculture and Technology, Pantnagar to assess the effect of age (one and two year old), growing condition (intermittent misting and no misting) and different seasons (summer, monsoon, autumn and spring) on rooting and shooting of culm cuttings of Dendrocalamus asper. The experiment was laid out in factorial randomized block design with three replications. The findings indicated that in D. asper, one year old culm cuttings in the intermittent misting condition showed good results. The findings also indicated that shooting and rooting per cent of culm cuttings planted in different season showed response as Spring (March)> Summer (June)> Monsoon (August)> Autumn (October).

scholarly journals Nutritional value of sorghum silages

2017 ◽  
Vol 39 (2) ◽  
pp. 137
Author(s):  
Marielly Maria Almeida Moura ◽  
Daniel Ananias de Assis Pires ◽  
Renê Ferreira Costa ◽  
Daniella Cangussú Tolentino ◽  
João Paulo Sampaio Rigueira ◽  
...  
Keyword(s):  
Crude Protein ◽  
Block Design ◽  
Significance Level ◽  
Dry Matter Digestibility ◽  
Randomized Block Design ◽  
Nutritional Characteristics ◽  
High Participation ◽  
Sorghum Genotypes

 This study aimed to evaluate the nutritional characteristics and quality of silages of five sorghum genotypes, namely: Volumax, AG2005E, Qualimax, BRS610 and AG2501. The study was conducted at the Experimental Farm Unimontes, in the municipality of Janaúba, state of Minas Gerais. The treatments were distributed in a randomized block design with four replicates. Data were tested by analysis of variance andmeans were compared by Scott-Knott test at a significance level of 5%. The pH values ranged from 3.93 and 4.10. Genotypes differed in all studied nutritional characteristics (p < 0.05). For the acid detergent fiber, differences were detected between the genotypes, AG2501 (35.66%), Volumax (34.89%), AG2005E (34.53%), Qualimax (32.39%) and BRS610 (33.60%). The high participation of lignin was verifiedin all genotypes except for Volumax (4.14%). All silages were within the recommended range 50-65% for in vitro dry matter digestibility and classified as having good quality, however only the AG2005E genotype met nitrogen requirements for microbial fermentation, with 7.06% crude protein. 

scholarly journals Optimation of In vitro Lateral Shoots Multiplication of Papaya (Carica papaya L.) “Callina” with BAP and NAA

2017 ◽  
Vol 4 (3) ◽  
pp. 102-107
Author(s):  
Darda Efendi ◽  
Mirza R Putra
Keyword(s):  
Carica Papaya ◽  
Clonal Propagation ◽  
Block Design ◽  
Shoot Multiplication ◽  
Plant Materials ◽  
Randomized Block Design ◽  
Lateral Shoots ◽  
Shoot Production ◽  
Set Up

Papaya is a popular fruit and is grown commercially in many subtropical and tropical countries. Papayas are generally grown from seeds; therefore the offsprings are not true-to-type and could come in three sexes, female, male, and hermaphrodite. Clonal propagation is required to obtain to grow true-to-type hermaphrodite papayas. In this research, we developed an in vitro protocol for shoot multiplication from lateral shoots from in vitro germinated papaya seedlings. The in vitro propagated plant materials could potentially be used as a source of papaya micro cuttings, or as scion for papaya grafting. The experiment was set up as a factorial experiment with NAA at 0, 0.1 and 0.5 mg.L-1, and BAP at 0, 0.1, 0.5, and 1.0 mg.L-1 in a completely randomized block design. BAP interacted with NAA in affecting the shoot production per explant. The optimum BAP and NAA concentration to produce lateral shoots was 0.54 mg. L-1 and 0.1 mg.L-1, respectively. Media without NAA reduced the number of lateral shoots and number of leaf per explant at any BAP concentration.Keywords: hermaphrodite, seeds, true to type, clonal propagation, micro cuttings.

scholarly journals Planting density and initial growth of two tree species adapted to the semi-arid region

2012 ◽  
Vol 36 (5) ◽  
pp. 951-960
Author(s):  
Paulo Sérgio Lima e Silva ◽  
Alexandre Emanuel Régis Holanda ◽  
Haroldo Nogueira de Paiva ◽  
Fábio Henrique Tavares de Oliveira ◽  
Odaci Fernandes de Oliveira
Keyword(s):  
Management Practices ◽  
Block Design ◽  
Gliricidia Sepium ◽  
Plant Age ◽  
Production Costs ◽  
Planting Density ◽  
Initial Growth ◽  
Randomized Block Design ◽  
Semi Arid Region ◽  
One Year

Planting densities influence several aspects of forest formation, including management practices, timber yield, quality, and extraction, and consequently its production costs. The objective of this study was to evaluate Mimosa caesalpiinifolia and Gliricidia sepium growth as a function of planting density (400, 600, 800, 1000, and 1200 plants ha-1) and plant age. The species were evaluated every 90 days for plant height (PH), crown diameter (CD) and root collar diameter (RCD) (10 cm above the ground), with the first evaluation performed at 90 days and the last at 720 days. When plants were one year of age and beyond, evaluations were conducted also for stem diameter at breast height (DBH) (1.30 m above the ground). A randomized block design with split-plots and three replicates was adopted. Species were assigned to plots, planting densities were assigned to subplots, and evaluation ages were assigned to subsubplots. The four traits in both species had their values decreased as planting density increased, but continually increased as plant age increased. For PH and RCD there was an alternation between species superiority, with gliricidia being superior to sabiá at some ages, while the opposite occurred at other ages. As to CD the species only differed in the last measurement, gliricidia being superior. With regard to DBH, gliricidia was superior starting from the second measurement. There was an effect of the species × ages interaction for the four traits and also an effect of the densities × ages interaction for CD and DBH.

scholarly journals Uji Efektivitas Rizobakteria Dalam Menghambat Perkembangan Penyakit Hawar Pelepah Daun (Rhizoctonia solani Kuhn.) Pada Padi Secara In Vitro

2020 ◽  
Vol 16 (1) ◽  
pp. 44
Author(s):  
Hanisa Desy Ariani ◽  
Noor Aidawati ◽  
Dewi Arika Adriani
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Rhizoctonia Solani ◽  
Plant Protection ◽  
Block Design ◽  
Sheath Blight ◽  
In Vitro Test ◽  
Sheath Blight Disease ◽  
Randomized Block Design ◽  
Blight Disease

One of the causes of the declining productivity of rice is sheath blight disease caused by the mold Rhizoctonia solani Kuhn. Control of sheath blight disease that is often done by the farmers is by using chemical pesticides (fungicides), which caused environmental problems. One way to reduce the use of pesticides is to biological control by using antagonist bacteria. This study aimed at in vitro test of rhizobacteria in preventing the development of sheath blight disease in rice plants. This research was conducted in the Phytopathology laboratory of Plant Protection Department of Faculty Agriculture, University of Lambung Mangkurat Banjarbaru from March to May 2018. The experiment used a randomized block design with three groups consisting of eight types of rhizobacteria isolates: (r1) Pseudomonas aeruginosa (Barito Kuala), (r2) Bacillus megaterium (Hulu Sungai Tengah), (r3) Azotobacter sp. (Barito Kuala), (r4) Pseudomonas sp. (Hulu Sungai Selatan), (r5) Flavobacterium sp. (Tanah Laut), (r6) Bacillus bodius (Barito Kuala), (r7) Pseudomonas aeruginosa (Hulu Sungai Selatan), (r8) Necercia sp. (Tanah Laut). The results showed that all rhizobacteria have the ability to inhibit the development of R. solani with different percentages of inhibitions. Pseudomonas aeruginosa (Barito Kuala) was the most effective rhizobacteria in inhibiting the development of R. solani.

scholarly journals The Effect of Processing and Cooling Methods on Coleus tuberosus in vitro Starch Digestibility

2021 ◽  
Vol 41 (3) ◽  
pp. 238
Author(s):  
Jhauharotul Muchlisyiyah ◽  
Tri Dewanti Widyaningsih ◽  
Retno Wulansari ◽  
Hera Sisca Prasmita
Keyword(s):  
Resistant Starch ◽  
Room Temperature ◽  
Potato Starch ◽  
Block Design ◽  
In Vitro Digestibility ◽  
Randomized Block Design ◽  
Two Factors ◽  
Cooling Methods ◽  
Total Starch

Coleus tuberosus, also known as black potato, is one of the Indonesian local tubers consumed as a carbohydrate substituent. Therefore, this study aimed to examine the effect of processing and cooling methods on the in vitro digestibility of black potato starch. Furthermore, two factors Randomized Block Design with a 2x3 experimental design was used, which consisted of processing methods (boiling, roasting, and microwave) and cooling at room temperature and 4 °C for 24 hours with 3 repetitions. Black potato flour was compared with the raw form, by assessing some parameters, namely Resistant Starch (RS), Slowly Digestible Starch (SDS), Rapidly Digestible Starch (RDS), and Glycemic Index (GI). Also, the analysis of total starch, moisture, and color was performed, hence raw black potatoes generally have 10% resistant starch (%wb). Different treatments of cooking and cooling had a significant effect (α = 0.05) on moisture content, total starch, RS, RDS, SDS, GI, brightness (L), and yellowness (b). Black potatoes subjected to the processing method followed by cooling had lower RDS and increased RS content. Furthermore, refrigeration at 4°C for 24 hours reduced the digestibility of black potato starch more than cooling at room temperature. Contrarily, microwaved black potato cooled at room temperature showed a higher digestion rate compared to the raw counterpart. Conclusively, processing followed by cooling reduces the GI and increases the RS content of Coleus tuberosus.

scholarly journals Grafting guava on cattley guava resistant to Meloidogyne enterolobii

2015 ◽  
Vol 45 (9) ◽  
pp. 1579-1584 ◽  
Author(s):  
Renata Rodrigues Robaina ◽  
Graziella Siqueira Campos ◽  
Cláudia Sales Marinho ◽  
Ricardo Moreira Souza ◽  
Cíntia Aparecida Bremenkamp
Keyword(s):  
Block Design ◽  
Promising Method ◽  
Initial Growth ◽  
Success Rates ◽  
Canopy Development ◽  
Randomized Block Design ◽  
Meloidogyne Enterolobii ◽  
Graft Success ◽  
One Year

<p>The use of resistant rootstocks could be a promising method to control nematode<bold>Meloidogyne enterolobii</bold>in commercial plantations of guava. The present study aimed to evaluate the success of grafting guava as a scion on accessions of cattley guava as rootstocks resistant to<bold> M. enterolobii.</bold>The treatments consisted of the rootstocks cattley guava plants (three accessions of<bold> Psidium cattleyanum</bold>) and common guava (control). In the apical wedge grafting method, scion of Paluma cultivated variety was used. The experiment was arranged in a randomized block design with four treatments and five replicates, and eight plants per plot. The saplings produced as described before were planted in the field where the initial growth of the different combinations were evaluated. Graft success was observed for the control (common guava) and for accessions 115 and 117 of cattley guava plants, with success rates of 63, 32 and 29%, respectively. In the field, the cattley guava used as rootstocks hampered Paluma canopy development and caused death of plants. Incompatibility of <bold>P. cattleyanum</bold>as rootstocks for<bold> P. guajava</bold>Paluma was confirmed one year after cultivation in field.</p>

scholarly journals Morphophysiology of Tahiti lime grafted onto Sunki mandarin hybrids under salt stress

2019 ◽  
Vol 23 (8) ◽  
pp. 598-606
Author(s):  
Luderlândio A. Silva ◽  
Marcos E. B. Brito ◽  
Pedro D. Fernandes ◽  
Francisco V. da S. Sá ◽  
Rômulo C. L. Moreira ◽  
...  
Keyword(s):  
Salt Stress ◽  
Saline Water ◽  
Block Design ◽  
Flowering Stage ◽  
Gas Exchanges ◽  
Rangpur Lime ◽  
Randomized Block Design ◽  
Irrigation Water Salinity ◽  
Electrical Conductivities ◽  
One Year

ABSTRACT This study aimed to evaluate the growth and physiology of citrus scion/rootstock combinations irrigated with saline water until the pre-flowering stage. The experiment was conducted in drainage lysimeters with capacity for 150 dm3, in randomized block design in a 2 x 10 factorial scheme, corresponding to two electrical conductivities of water (S1 = 0.3 and S2 = 3.0 dS m-1) and ten scion/rootstock combinations (nine hybrids and one commercial variety) grafted with Tahiti lime, in three repetitions and one plant per plot. Grafted seedlings were transplanted one year after sowing, subjected to salt stress from 15 days after transplanting until the pre-flowering period, and evaluated for gas exchanges and growth. The irrigation with 3.0 dS m-1 saline water did not influence the photosynthetic activity of the studied citrus scion/rootstock combinations until the pre-flowering. The genotype Santa Cruz Rangpur lime (LCRSTC) was more sensitive to irrigation water salinity in terms of growth. The least sensitive combinations to salinity were Tahiti lime grafted onto TSKFL x (LCR x TR) - 018, TSKFL x TRBK - 011 and TSKFL x TRBK - 30.

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