THE EFFECT OF DIFFERENT DOSES OF OESTRADIOL-17β ON COLLAGEN SYNTHESIS IN THE FEMUR OF CASTRATED FEMALE RATS

1975 ◽  
Vol 80 (3) ◽  
pp. 613-624 ◽  
Author(s):  
Norvald Langeland
Keyword(s):  
Specific Activity ◽  
Mature Female ◽  
The Body ◽  
Collagen Metabolism ◽  
Collagen Content ◽  
Female Rats ◽  
Bone Collagen ◽  
Tissue Fluid ◽  
Body Weights ◽  
Different Doses

ABSTRACT The effect of different doses of oestradiol-17β on collagen metabolism in the femur of castrated young mature female rats was studied. The animals received daily injections of the hormone for 21 days and 25 μCi [14C]proline was injected intraperitoneally 24 h before the rats were sacrificed. The rats receiving 1 μg oestradiol per day had a significantly higher specific activity of hydroxyproline in bone compared to the normal and the castrated control rats. At the same time the bone weights and collagen content per femur were less in the rats receiving oestradiol 1 and 2 μg/day than in the control animals. The higher specific activity of bone hydroxyproline in the rats receiving oestradiol-17β 1 μg/day did not, however, indicate a higher bone collagen accretion rate. As all rats received the same amount of [14C] proline, and the body weights differed considerably at sacrifice, the animals probably had different tissue fluid concentrations of [14C] proline. This possibility was supported by the observation that serum concentrations of radioactivity were inversely correlated to body weights. "Correcting" for differences in body weights, the castrated control rats turned out to be the group with the highest specific activity. On doses of 10 and 20 μg oestradiol-17β per day, the specific activity of hydroxyproline in bone was lower than in the control rats while bone weights and collagen content of the femur were not different from the corresponding values of the castrated controls. This finding suggests a "slow down" of collagen metabolism – both with regard to the accretion and the resorption. At high doses – 10 and 20 μg oestradiol per animal per day – the percentage of incorporated 14C found as [14C]hydroxproline was reduced. The reason for this altered total-C14/[14C]hydroxyproline ratio is at present unknown.

IN VITRO STUDIES ON COLLAGEN METABOLISM IN METAPHYSEAL RAT BONE

1975 ◽  
Vol 80 (4) ◽  
pp. 795-800 ◽  
Author(s):  
Norvald Langeland ◽  
Vigdis Teig
Keyword(s):  
Collagen Synthesis ◽  
Mature Female ◽  
Parathyroid Glands ◽  
Collagen Metabolism ◽  
Female Rats ◽  
Bone Collagen ◽  
Detectable Effect ◽  
Bone Collagen Synthesis ◽  
Rat Bone

ABSTRACT Oestradiol-17β has been administered to hypophysectomized/castrated and to thyro-parathyroidectomized/castrated young mature female rats. Treatment with oestradiol-17β 5 μg/day per animal, was continued for 3 weeks. Bone pieces from tibia/femur metaphyses were incubated in vitro for 3 h in order to establish the bone collagen synthesis and resorption rates. The results were compared to results from a previous study on castrated female rats with intact hypophysis, thyroid and parathyroid glands. Bone from the thyro-parathyroidectomized/castrated rats treated with oestradiol-17β had a significantly reduced collagen resorption rate in vitro as compared to their paired controls. This is a result consistent with that of castrated female rats with intact thyroid and parathyroid glands treated with oestradiol. Oestradiol-17β had no detectable effect upon collagen metabolism in the hypophysectomized/castrated rats. It is concluded that the effect of oestradiol on bone resorption is not exerted via the parathyroid glands or parathyroid hormone. The possibility that oestrogens may act on bone via the hypophysis is discussed.

EFFECTS OF ADRENALECTOMY ON THE RELEASE OF FOLLICLE-STIMULATING HORMONE AND THE ONSET OF PUBERTY IN FEMALE RATS

1977 ◽  
Vol 75 (3) ◽  
pp. 419-426 ◽  
Author(s):  
H. M. A. MEIJS-ROELOFS ◽  
P. KRAMER
Keyword(s):  
Body Weight ◽  
Adrenal Gland ◽  
Follicle Stimulating Hormone ◽  
The Body ◽  
Biologically Active ◽  
Female Rats ◽  
Vaginal Opening ◽  
Uterine Weight ◽  
Body Weights ◽  
Adrenalectomized Rats

The involvement of the adrenal gland in the release of gonadotrophins and the onset of puberty in female rats was studied. Two and four days after adrenalectomy (ADX) on either day 5 or 10 after birth, a significant decrease in the concentration of FSH was found; 4 days after ADX on either day 15 or 20, FSH concentrations had increased significantly compared with sham-operated and/or intact controls. However, in the rats adrenalectomized on day 15 or 20, the body weights were lower than in control rats. Relative uterine weights (mg/100 g body wt) in adrenalectomized rats never differed from those of control rats. A delay in the time at which vaginal opening and the first oestrus occurred was found in rats adrenalectomized at 20 or 25 days of age; however this delay was accompanied in these rats by a retardation in the gain in body weight. It is argued that the effects of ADX on both the release of gonadotrophins and the onset of puberty are primarily, and presumably exclusively, due to the effects on general bodily development (expressed in body weight). The lack of effect of ADX on uterine weight supports the hypothesis that 'oestrogen-like' products from the adrenal gland are not biologically active as oestrogens.

IN VITRO STUDIES ON COLLAGEN METABOLISM IN METAPHYSEAL RAT BONE

1975 ◽  
Vol 80 (4) ◽  
pp. 775-783 ◽  
Author(s):  
Norvald Langeland
Keyword(s):  
Amino Acid ◽  
Collagen Synthesis ◽  
Treated Group ◽  
In Vitro Studies ◽  
Collagen Metabolism ◽  
Bone Collagen ◽  
Accretion Rates ◽  
Different Doses ◽  
Rat Bone

ABSTRACT The effect of oestradiol-17β treatment on bone collagen metabolism in vitro was studied in metaphyseal rat bone. Rats were oophorectomized and subsequently treated for 3 weeks with different doses of oestradiol-17β. Bone pieces were incubated in a modified Krebs-Ringer bicarbonate medium for 6 h. Synthesis of [14C] hydroxyproline from [14C]proline and the incorporation of this amino acid into the bone samples was determined and the collagen synthesis and accretion rates calculated from these data. Collagen resorption rates were calculated from measured release of non-radioactive hydroxyproline to the medium. Castration resulted in an increased rate of accretion and resorption of collagen. All doses of oestradiol tested in this study (1 to 20 μg per animal per day for 3 weeks) decreased both accretion and resorption rates to levels insignificantly different from those of the non-castrated control rats. Only the 2 μg treated group had significantly better collagen balance than the castrated untreated rats.

Maternal dietary deficiency and its effect on the metabolism of nucleic acids and proteins. "Effect of exchanging the young, during the lactation period, between the control and undernourished female rats"

1978 ◽  
Vol 56 (2) ◽  
pp. 274-286 ◽  
Author(s):  
Tapas Goswami ◽  
Uma Srivastava
Keyword(s):  
Nucleic Acids ◽  
Protein Content ◽  
Specific Activity ◽  
The Body ◽  
Female Rats ◽  
Control Group ◽  
Lactation Period ◽  
Specific Activities ◽  
The Brain ◽  
Dietary Deficiency

The effect of maternal dietary deficiency on the metabolism of nucleic acids and proteins was studied by exchanging the pups of control and undernourished dams during the lactation period. In the pups of control dams fostered by undernourished dams during the lactation period (E3), it was observed that the body and organ weight, and RNA, DNA, and protein content failed to increase normally. Contrary to this, the free leucine and nucleotide contents were higher and their specific activities lower in the plasma and various organs of the E3 group as compared with the control group.Specific activity of protein was higher in the liver, brain, kidney, and lung, and was lower in the spleen and heart of the E3 group as compared with the control group. Specific activity of RNA was higher in the liver, spleen, and lung, and was lower in the brain, kidney, and heart of the E3 group as compared with the control group.In the pups of undernourished dams fostered by the control dams during the lactation period (E1), the body and organ weights, the RNA, DNA, and protein content, the content of free leucine and nucleotides as well as their specific activities, and the specific activity of protein and RNA were partially or completely restored. However, the DNA content of the brain remained unchanged in comparison with those pups of undernourished dams nursed by their own mother (E2). In the brain, kidney, spleen, and lung of the E1 group, the specific activity of RNA increased considerably and even exceeded the control values.The radioactivity results discussed above clearly demonstrate an accelerated metabolism of protein and RNA in the various organs of the E3 group and a partial or complete normalization in the E1 group.

scholarly journals EFFECT OF THE ROOTS OF ALGERIAN BUNIUM INCRASSATUM ON BIOLOGICAL, BIOCHEMICAL AND HISTOLOGICAL PARAMETERS OF MATURE FEMALE RATS

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
Nawel Attoui ◽  
Farid Berroukeche ◽  
Kamilia Guedri ◽  
Fethi Toul
Keyword(s):  
Biochemical Parameters ◽  
Histological Study ◽  
Mature Female ◽  
The Body ◽  
Female Rats ◽  
Standard Diet ◽  
Female Wistar Rats ◽  
The Impact ◽  
Positive Effect ◽  
As Effects

The present study’s objective was to evaluate the impact of Bunium incrassatum roots’ powder (Talghouda) as a dietary supplement on the evolution of biological, biochemical, and histological parameters in female Wistar rats. The animals were divided into two groups and given an orally standard diet supplemented with 15% of Bunium incrassatum roots powder, daily for 15 days. The obtained results showed that the roots of Bunium incrassatum induced an increase in the body and organs weight of Diet rats compared to the control, as well as effects on the biochemical parameters, characterized mainly by a significant increase in the glycaemia (+44.44%), triglyceride (+56.81%), cholesterol (+11.90%), LDL (+4.08%) and HDL (+11.53%)and a decrease in creatinine (-3.65%), TGO (-6.92%) and TGP (-58.73%). In the histological study of organs, the thyroid of diet rats revealed large thyroid follicles, the colloid of the follicles was more or less developed compared to the control rats. Through the obtained results, it can be concluded that a standard diet supplemented with 15% of Bunium incrassatum roots powder may have a positive effect on biological, biochemical, and histological parameters

IN VITRO STUDIES ON COLLAGEN METABOLISM IN METAPHYSEAL RAT BONE

1975 ◽  
Vol 80 (4) ◽  
pp. 784-794 ◽  
Author(s):  
Norvald Langeland ◽  
Vigdis Teig
Keyword(s):  
Incorporation Rate ◽  
In Vitro Studies ◽  
Collagen Metabolism ◽  
Female Rats ◽  
Bone Collagen ◽  
Metaphyseal Bone ◽  
Rat Bone

ABSTRACT In vitro collagen metabolism in metaphyseal bone has been studied during the first few hours and days after the start of in vivo treatment of castrated female rats with various doses of oestradiol-17β. Already during the first 2 h after the start of treatment with oestradiol-17β 2 μg/day and within the first 24 h in the rats receiving 20 μg/day, a reduction of the collagen incorporation rate was demonstrated. The incorporation rates remained at approximately the same reduced level during the following 3 weeks. In rats receiving oestradiol-17β 1 μg/day no such reduced incorporation rate could be demonstrated during the first 36 h after the start of treatment. The bone collagen resorption rate was significantly elevated during the first 3 days of treatment in the animals receiving oestradiol-17β both 1 and 20 μg/day. After this time the resorption rates gradually fell to levels significantly below controls at 3 weeks after start of treatment.

scholarly journals Effect of neonatal undernutrition on serum follicle-stimulating hormone levels and ovarian development in the female rat

1980 ◽  
Vol 44 (2) ◽  
pp. 179-182 ◽  
Author(s):  
P. E. Schenck ◽  
A. Koos Slob ◽  
J. TH. J. Uilenbroek ◽  
J. J. Van Der Werff Ten Bosch
Keyword(s):  
Ovarian Development ◽  
Slow Growth ◽  
Follicle Stimulating Hormone ◽  
The Body ◽  
Female Rats ◽  
Female Rat ◽  
Maximal Diameter ◽  
Body Weights ◽  
High Level ◽  
Stimulating Hormone

1. Female rats were undernourished from birth through replacement for 12 h/d of the lactating mother by a nipple-ligated mother. This resulted in slow growth so that at 22 d of age the body-weights equalled those of well-nourished animals at 14 d.2. At 14, 17, 20 and 22 d groups of eight to ten animals each of undernourished and well-nourished rats were autopsied, serum follicle-stimulating hormone (FSH) concentration was determined by radioimmunoassay, utersuses were weighed and ovaries examined by light microscopy.3. Undernutrition caused a 3–4 d delay in the third-week drop in serum FSH and in ovarian development as indicated by the number of antral follicles and by the maximal diameter of the ovaries and of the largest follicles.4. It is postulated that the effect of undernutrition on serum FSH may be due to delayed increase in ovarian feedback effectiveness, which may be the result of either the retardation in ovarian development or a continued high level of serum oestrogen-binding protein. It remains uncertain through which mechanism(s) undernutrition brings about delayed onset of puberty.

Estrogenic Activity Produced by Aqueous Extracts of Silkworm (Bombyx mori) Pupae in Ovariectomized Rats

2010 ◽  
Vol 38 (01) ◽  
pp. 89-97 ◽  
Author(s):  
Hyo-Jung Yang ◽  
Jeong-Woong Lee ◽  
So-Hyun Lee ◽  
Jae-Sung Ryu ◽  
Dong-Hoon Kwak ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Estrogenic Activity ◽  
The Body ◽  
Female Rats ◽  
Ovariectomized Rats ◽  
Aqueous Extracts ◽  
Ovx Rats ◽  
Silkworm Pupae ◽  
Body Weights ◽  
Silkworm Bombyx Mori

This study examined the estrogenic activity produced by aqueous extracts of silkworm (Bombyx mori) pupae in ovariectomized (OVX) rats. The components of silkworm pupae were extracted in distilled water at room temperature for 6 hours. The ovaries of six-week old female rats were then bilaterally removed. One week after OVX, the animals were treated with 200, 400 or 600 mg/kg/day of silkworm pupae extracts. The body weights of the OVX rats increased remarkably compared to the control rats, however their relative uterus weights to body weights decreased significantly. Treatment with the aqueous extracts of silkworm pupae dramatically improved the decreased uterus weights of OVX rats, with the highest increase observed in treatment with 200 mg/kg/day of the aqueous extracts. Additionally, treatment with aqueous extracts (200 mg/kg/day) of silkworm pupae significantly elevated the serum 17β-estradiol contents of OVX rats when compared to the control animals. To examine the toxic effects of silkworm pupae on the hepatic functions of OVX rats, the levels of serum glutamate oxaloacetate transaminase (GOT) and glutamate pyruvate transaminase (GPT) were measured. The serum GOT and GPT levels did not change in response to the administration of aqueous extracts (200, 400 and 600 mg/kg/day) for 4-weeks. Taken together, these results suggest that the aqueous extracts of silkworm pupae may have estrogenic activity, which suggests that silkworm pupae may be useful in the prevention and/or treatment of menopausal disorders caused by deficiencies in female sexual hormones, including estrogen.

EFFECTS OF OESTRADIOL-17β BENZOATE TREATMENT ON FRACTURE HEALING AND BONE COLLAGEN SYNTHESIS IN FEMALE RATS

1975 ◽  
Vol 80 (3) ◽  
pp. 603-612 ◽  
Author(s):  
Norvald Langeland
Keyword(s):  
Tensile Strength ◽  
Young Adult ◽  
Collagen Synthesis ◽  
Specific Activity ◽  
Female Rats ◽  
Bone Collagen ◽  
Fracture Region ◽  
Significant Difference ◽  
High Doses ◽  
Bone Collagen Synthesis

ABSTRACT The influence of oestradiol-17β benzoate, 70 μg administered intramuscularly twice a week for up to four weeks, on collagen synthesis in fractured bones, was studied in young adult castrated female rats. The specific activity of [14C] hydroxyproline in the fracture region of the treated rats was constantly below that of the normal and the castrated control animals throughout the experimental periods. The percentage of incorporated 14C found as [14C] hydroxyproline was also reduced in the fracture regions of the oestrogen treated rats compared to the percentage observed in normal rats. During the first two weeks after fracture and the start of treatment, there was no differences in tensile strength of the fractures and no significant difference in collagen content of the fractured tibia of the 3 experimental groups. The weights of the unfractured tibiae in oestrogen treated rats were lower than those of the control animals. The findings suggest a "slow down" of collagen metabolism after administration of high doses of oestradiol.

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