scholarly journals Allopregnanolone concentrations and premenstrual syndrome

2000 ◽  
pp. 269-273 ◽  
Author(s):  
P Monteleone ◽  
S Luisi ◽  
A Tonetti ◽  
F Bernardi ◽  
AD Genazzani ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Specific Antibody ◽  
Premenstrual Syndrome ◽  
Luteal Phase ◽  
Test Design ◽  
Normal Range ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Gaba A ◽  
Psychological Conditions

OBJECTIVE: To evaluate basal allopregnanolone and progesterone in both phases of the menstrual cycle in women suffering from premenstrual syndrome (PMS) and their response to a GnRH test. DESIGN: We selected 56 women (28 patients with PMS and 28 controls) aged between 18 and 32 years. Blood samples were drawn in both follicular and phases. Twenty-eight women (14 patients with PMS and 14 controls) underwent a GnRH test in the luteal phase. METHODS: We evaluated allopregnanolone by RIA, using a specific antibody. Serum progesterone and oestradiol were determined using a commercially available RIA kit. RESULTS: Luteal phase allopregnanolone concentrations were significantly lower in patients with PMS than in controls. Progesterone concentrations were significantly lower in patients with PMS in both the follicular and the luteal phase. Serum oestradiol concentrations were in the normal range in both groups of women, although slightly greater in those with PMS. Allopregnanolone and progesterone responses to a GnRH test were significantly blunted in women with PMS. CONCLUSIONS: Diminished concentrations of allopregnanolone and progesterone, its precursor, and a blunted response to the GnRH test lead us to hypothesise that patients with PMS may suffer from an inadequate production of ovarian neuroactive steroids, especially in the luteal phase. This would lead to an impaired anxiolytic GABA(A)-mediated response in stressful physiological and psychological conditions, and may in part explain various psychoneuroendocrine symptoms that arise during PMS.

Influence of PMSG antiserum on serum progesterone and estrous cycles of PMSG-treated gilts

1992 ◽  
Vol 72 (1) ◽  
pp. 173-176 ◽  
Author(s):  
R. N. Kirkwood ◽  
P. A. Thacker
Keyword(s):  
Key Words ◽  
Luteal Phase ◽  
Ovarian Activity ◽  
Serum Estradiol ◽  
Serum Progesterone ◽  
Estrous Cycles ◽  
Blood Samples ◽  
Effect Of Treatment

Forty-seven prepubertal gilts (88.4 ± 0.9 kg) received an injection of 750 IU PMSG to stimulate ovarian activity. After 96 h, 23 gilts received an injection of an anti-PMSG serum. Blood samples were obtained at the time of PMSG injection (day 0) and at 2, 4, 6, 11, 18, 25 and 32 d. There was no effect of treatment on serum estradiol concentrations or on the occurrence of normal estrous cycles. However, luteal phase serum progesterone concentrations were higher (P < 0.01) in gilts receiving the anti-PMSG. Key words: PMSG, antiserum, gilts, ovulation

scholarly journals Relationship between Anxiety and Interleukin 10 in Female Soccer Players with and Without Premenstrual Syndrome (PMS)

2017 ◽  
Vol 39 (11) ◽  
pp. 602-607 ◽  
Author(s):  
Roberta Foster ◽  
Mauro Vaisberg ◽  
Maíta Araújo ◽  
Marcia Martins ◽  
Tiago Capel ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Premenstrual Syndrome ◽  
Luteal Phase ◽  
Pearson Correlation ◽  
Interleukin 10 ◽  
Soccer Players ◽  
Test Analysis ◽  
Symptom Report ◽  
Significance Level ◽  
Simulated Game

Objective To investigate the level of anxiety and its relationship with interleukin (IL)-10 (anti inflammatory cytokine that modulates mood swings) in a group of female soccer players. Methods Fifty-two eumenorrheic soccer players were evaluated (age 19.8 ± 4.7 years). The presence of premenstrual syndrome (PMS) and phases of the menstrual cycle were determined by a daily symptom report (DSR) kept for 3 consecutive months. The concentration of cytokine IL-10 was determined from urine samples collected at four moments: at the follicular and luteal phases of the menstrual cycle, and before (pre) and after (post) the simulated game, and it was quantified by flow cytometry (Luminex xMAP - EMD Millipore, Billerica, MA, USA). The level of anxiety was determined through the BAI anxiety questionnaire answered by all athletes at the same time of the urine collection. The Student t-test, analysis of variance (ANOVA) and Pearson correlation with significance level at 5% were used for data analysis. Results We showed that the prevalence of PMS among female soccer players is similar to that reported in the literature. In addition, we showed that the group with PMS has a higher level of anxiety compared with group without PMS (p = 0.002). Interleukin-10 analysis in players without PMS revealed that there was a significant decrease in the level of this cytokine before the game during the luteal phase when compared with the follicular phase (p < 0.05). The correlation analysis between IL-10 and anxiety showed a negative correlation post-game in the luteal phase in the group without PMS (p = 0.02; r = -0.50) and a positive correlation post-game in the luteal phase in PMS group (p = 0.04; r = 0.36). Conclusion Our results suggest that IL-10 may contribute to reduce anxiety in the group without PMS. This could be attributed to the fact that no IL-10 variation was observed in the group with PMS, which presented higher anxiety symptoms when compared with the group without PMS.

scholarly journals Blood Ghrelin, Adiponectin and Resistin Levels During Controlled Ovarian Stimulation in IVF Cycles

2016 ◽  
pp. 809-814
Author(s):  
K. DAFOPOULOS ◽  
C. I. MESSINI ◽  
G. ANIFANDIS ◽  
P. GEORGOULIAS ◽  
D. SOURLAS ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Gnrh Agonist ◽  
Ovarian Stimulation ◽  
Luteal Phase ◽  
Controlled Ovarian Stimulation ◽  
Recombinant Fsh ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Stimulation Period ◽  
First Time

The aim of the present study was to investigate changes of blood ghrelin, adiponectin and resistin levels in IVF/ICSI-ET cycles. Twenty women were stimulated with recombinant FSH in a GnRH agonist short protocol for IVF/ICSI. Blood samples were taken on cycle day 2 before the commencement of injections, on cycle day 6 and on the days of HCG injection, oocyte pick up (OPU), embryo transfer (ET) as well as 7 and 12 days post-ET. Serum E2 levels increased during the stimulation, peaking on the HCG day and declined thereafter (p<0.001). Serum progesterone levels started to increase on the OPU day, peaking on the ET day (p<0.001) and decreased on days 7 and 12 post-ET. Plasma ghrelin remained unchanged during the whole cycle. Serum adiponectin levels remained stable during the stimulation period until the ET day and decreased on days 7 and 12 post-ET (p<0.001). Serum resistin levels increased until the ET day (p<0.05), remained unchanged on day 7 post-ET and decreased on day 12 post-ET (p<0.05). The present study shows for the first time that ghrelin levels did not change significantly during IVF/ICSI-ET cycles. Resistin levels increased during the stimulation period while adiponectin levels remained stable decreasing during the luteal phase.

Hypercapnic blood pressure response is greater during the luteal phase of the menstrual cycle

1996 ◽  
Vol 81 (5) ◽  
pp. 2142-2146 ◽  
Author(s):  
N. Edwards ◽  
I. Wilcox ◽  
O. J. Polo ◽  
C. E. Sullivan
Keyword(s):  
Blood Pressure ◽  
Menstrual Cycle ◽  
Luteal Phase ◽  
Blood Pressure Response ◽  
Cardiovascular Responses ◽  
Follicular Phase ◽  
Pressure Response ◽  
Serum Progesterone ◽  
Menstrual Cycles ◽  
Blood Pressure Responses

Edwards, N., I. Wilcox, O. J. Polo, and C. E. Sullivan.Hypercapnic blood pressure response is greater during the luteal phase of the menstrual cycle. J. Appl. Physiol. 81(5): 2142–2146, 1996.—We investigated the cardiovascular responses to acute hypercapnia during the menstrual cycle. Eleven female subjects with regular menstrual cycles performed hypercapnic rebreathing tests during the follicular and luteal phases of their menstrual cycles. Ventilatory and cardiovascular variables were recorded breath by breath. Serum progesterone and estradiol were measured on each occasion. Serum progesterone was higher during the luteal [50.4 ± 9.6 (SE) nmol/l] than during the follicular phase (2.1 ± 0.7 nmol/l; P < 0.001), but serum estradiol did not differ (follicular phase, 324 ± 101 pmol/l; luteal phase, 162 ± 71 pmol/l; P = 0.61). The systolic blood pressure responses during hypercapnia were 2.0 ± 0.3 and 4.0 ± 0.5 mmHg/Torr (1 Torr = 1 mmHg rise in end-tidal [Formula: see text]) during the follicular and luteal phases, respectively, of the menstrual cycle ( P < 0.01). The diastolic blood pressure responses were 1.1 ± 0.2 and 2.1 ± 0.3 mmHg/Torr during the follicular and luteal phases, respectively ( P < 0.002). Heart rate responses did not differ during the luteal (1.7 ± 0.3 beats ⋅ min−1 ⋅ Torr−1) and follicular phases (1.4 ± 0.3 beats ⋅ min−1 ⋅ Torr−1; P = 0.59). These data demonstrate a greater pressor response during the luteal phase of the menstrual cycle that may be related to higher serum progesterone concentrations.

Measurement of Activated Protein C Resistance during Menstrual Cycle in Women with and without the Leiden Mutation

2001 ◽  
Vol 85 (04) ◽  
pp. 614-618 ◽  
Author(s):  
K. Bremme ◽  
M. Blombäck ◽  
M. L. Wramsby
Keyword(s):  
Menstrual Cycle ◽  
Luteal Phase ◽  
Protein C ◽  
Activated Protein C ◽  
Factor V ◽  
Activated Protein C Resistance ◽  
Blood Samples ◽  
Plasma Methods

SummaryVariations in the APC ratio during the menstrual cycle were assessed in 25 women without the Leiden mutation, and 10 women who were carrier of the mutation. Blood samples were collected at four occasions during one menstrual cycle. APC ratios were measured with two APTT based plasma methods with and without factor V depleted plasma.None of the methods were able to accurately discriminate between mutated and non mutated women in all samples. Although a normalized method with factor V depleted plasma was favorable.The levels of estradiol and progesterone did not differ between mutated and non mutated women.Our findings suggest that the level of estradiol at estimated time of ovulation is of importance for the response to APC during luteal phase, since the women exhibiting the highest levels of estradiol at time for ovulation had the lowest response to APC.

A study of the luteolytic mechanism of the antiprogesterone RU486 during the late-luteal phase in pseudopregnant rats

1996 ◽  
Vol 150 (1) ◽  
pp. 93-98 ◽  
Author(s):  
Y Nakamura ◽  
H Tamura ◽  
M Ono ◽  
K Shimamura ◽  
N Sugino ◽  
...  
Keyword(s):  
Subcutaneous Injection ◽  
Luteal Phase ◽  
Treated Group ◽  
Sesame Oil ◽  
Serum Prolactin ◽  
Control Group ◽  
Serum Progesterone ◽  
Blood Samples ◽  
Serial Blood ◽  
Late Luteal Phase

Abstract The purpose of this study was to examine the possible mechanism through which RU486 induces luteolysis during the late-luteal phase in pseudopregnant (PSP) rats. PSP rats received a subcutaneous injection of RU486 in sesame oil (5 mg/kg body weight) or sesame oil alone once a day between day 9 and day 11 of pseudopregnancy. Serial blood samples were collected on days 5, 9, 10, 11 and 12 and assayed for progesterone content. To examine the possible action of RU486 through a uterine and/or a pituitary (prolactin-dependent) mechanism, PSP rats and chronic hysterectomized PSP rats which had been hysterectomized before PSP induction received a subcutaneous injection of RU486 in sesame oil (5 mg/kg body weight), sesame oil alone, prolactin in 50% polyvinylpyrrolidone (15 IU/day), or RU486 and prolactin once a day between day 9 and day 11 of pseudopregnancy. Serial blood samples were collected on days 5, 9, 10 and 11 and assayed for progesterone content. Blood samples were also collected at 0400 h on day 12 and used for prolactin and progesterone determinations. To examine the direct effect of RU486 on corpus luteum and/or pituitary, hysterectomized rats underwent hypophysectomy and pituitary autotransplantation on dioestrus 1 and received a subcutaneous injection of RU486 in sesame oil or sesame oil alone for 3 days between day 21 and day 23 after surgery. Serial blood samples were collected on days 10, 21, 22, 23 and 24 and assayed for progesterone and prolactin contents. In ordinary PSP rats, serum progesterone levels were significantly (P<0·01) lower in the RU486-treated group than in the control group (9 ± 1 vs 53 ± 7 ng/ml; mean ± s.e.m.) on day 11. Serum prolactin levels at 0400 h on day 12 of pseudopregnancy were significantly (P<0·05) lower in the RU486-treated group than in the control group (16 ±4 vs 154 ±44 ng/ml; mean ± s.e.m.). The concomitant prolactin treatment reversed the luteolytic effects of RU486 on day 11 of pseudopregnancy. In hysterectomized PSP rats, RU486 also suppressed serum prolactin levels, and the concomitant prolactin treatment again reversed the luteolytic effects of RU486. In hysterectomized rats which were hypophysectomized and pituitary autotransplanted, RU486 treatment did not induce any significant changes in serum progesterone and prolactin levels. These results indicated that RU486 induced luteolysis during the late-luteal phase in PSP rats by suppressing prolactin secretion via a hypothalamic mechanism. Journal of Endocrinology (1996) 150, 93–98

scholarly journals Neuroactive steroid-serotonergic interaction: responses to an intravenous L-tryptophan challenge in women with premenstrual syndrome

2001 ◽  
pp. 25-33 ◽  
Author(s):  
N Rasgon ◽  
M Serra ◽  
G Biggio ◽  
MG Pisu ◽  
L Fairbanks ◽  
...  
Keyword(s):  
Menstrual Cycle ◽  
Body Mass ◽  
Premenstrual Syndrome ◽  
Luteal Phase ◽  
Neuroactive Steroids ◽  
Serotonergic System ◽  
Neuroactive Steroid

OBJECTIVE: To evaluate the circulating concentrations of the neuroactive steroids in response to an i.v. L-tryptophan (L-TP) challenge across the menstrual cycle in women with premenstrual syndrome (PMS) and in controls. METHOD: An i.v. L-TP challenge was administered eight times during 1 month to five women with prospectively documented PMS and five age- and body mass-matched controls. Progesterone, allopregnanolone pregnenolone and 3alpha-5alpha-tetrahydrocorticosterone were assessed 15 and 0 min before, and at 30, 60 and 90 min after the challenge, across the menstrual cycle. RESULTS: In response to L-TP challenge, only allopregnanolone concentrations were significantly increased across the cycle and this increase was of a greater magnitude in women with PMS. Pregnenolone and 3alpha-5alpha-tetrahydrocorticosterone concentrations were not affected in women with PMS or controls after L-TP challenge. CONCLUSIONS: The data provide evidence for possible interaction between the serotonergic system and the neuroactive steroid, allopregnanolone. Women with PMS demonstrated a more significant increase in allopregnanolone concentrations in response to L-TP challenge, which could be due to an initial low basal serotonergic tone in the luteal phase in the PMS group.

scholarly journals HUBUNGAN ANTARA HIPERTENSI DENGAN PREMENSTRUAL SYNDROME PADA WANITA USIA REPRODUKTIF

Biomedika ◽  
2014 ◽  
Vol 6 (2) ◽  
Author(s):  
Azhim Rahmawati ◽  
Retno Siryaningsih ◽  
Safari Wahyu Jatmiko
Keyword(s):  
Risk Factor ◽  
Menstrual Cycle ◽  
Medical Care ◽  
Premenstrual Syndrome ◽  
Luteal Phase ◽  
Reproductive Age ◽  
Physical Disturbance ◽  
Chi Square ◽  
Cross Sectional ◽  
Chi Square Test

Premenstrual syndrome (PMS) is a cycle disorder which is commonly occured during the luteal phase of the menstrual cycle and will dissapear at the time of menstruation. Ninety percent of women on reproductive ages experience symptomps of PMS and 10% of them experience severe premenstrual symptomps that cuse physical disturbance, medical care necessity, even worst death. Hypertension is a risk factor for a PMS. The aim of this research is to find out a conection between PMS and hypertension. This research was designated using analytic observational with cross sectional approach. We used purposive sampling to get sample. Based on sample formula, we got 104 respondents which is consist of 52 women with hypertension and 52 women normotensive. To obtain data, we used shortened premenstrual syndrome assesment form (SPAF). Data collected were analyzed by chi square test. The result showed that the value of p = 0.00 (p<0.005). This result indicated that there is a conection between hypertension and PMS occurence in reproductive age. The probability of hypertension women to get PMS is 6.75.Keywords: Hypertension, Premenstrual Syndrome, reproductive age

PERIPHERAL PLASMA LEVELS OF 17α-HYDROXYPROGESTERONE, PROGESTERONE AND OESTRADIOL DURING NORMAL MENSTRUAL CYCLES IN WOMEN

1972 ◽  
Vol 71 (4) ◽  
pp. 743-754 ◽  
Author(s):  
Tore H:son Holmdahl ◽  
Elof D. B. Johansson
Keyword(s):  
Menstrual Cycle ◽  
Plasma Levels ◽  
Luteal Phase ◽  
Cycle Length ◽  
Gel Chromatography ◽  
Blood Samples ◽  
Menstrual Cycles ◽  
Peripheral Plasma ◽  
Competitive Protein Binding ◽  
Human Menstrual Cycle

ABSTRACT Liquid-gel chromatography on hydroxyalkoxypropyl Sephadex has been used to separate 17α-hydroxyprogesterone* and progesterone from interfering steroids prior to assay by competitive protein binding. During the luteal phase 0.5 ml of plasma was enough for determinations of both steroids. Fifteen samples could be assayed in less than 48 h. Oestradiol in plasma was assayed by radioimmunoassay. Daily blood samples were collected during 10 normal menstrual cycles in young, healthy women. The average cycle length was 29.7±2.0 (sd) days. 17α-hydroxyprogesterone displayed a midcyclic peak averaging 1.86±0.70 (sd) ng per ml coinciding with a midcyclic peak of oestradiol averaging 16.50± 5.95 (sd) ng per 100 ml of plasma. The highest luteal phase level of 17α-hydroxyprogesterone was 1.94 ±0.72 (sd) ng per ml. The corresponding levels for oestradiol were 9.1 ±3.8 (sd) ng per 100 ml. Progesterone formed a luteal plateau averaging 12.3±2.3 (sd) days. The highest luteal level of progesterone was 14.6±2.1 (sd) ng per ml. The peripheral plasma pattern of 17α-hydroxyprogesterone seems to mimic that of oestradiol during the human menstrual cycle.

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