scholarly journals SKRINING FITOKIMIA DAN PENETAPAN KADAR FENOL TOTAL PADA EKSTRAK DAUN NANGKA (Artocarpus heterophyllus), CEMPEDAK (Artocarpus integer), dan TARAP (Artocarpus odoratissimus) ASAL DESA PENGARON KABUPATEN BANJAR

2021 ◽  
Vol 4 (1) ◽  
pp. 95-102
Author(s):  
Muhammad Ikhwan Rizki ◽  
◽  
Nurlely Nurlely ◽  
Fadlilaturrahmah Fadlilaturrahmah ◽  
Ma’shumah Ma’shumah
Keyword(s):  
Abdominal Pain ◽  
Secondary Metabolites ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phytochemical Screening ◽  
Phenol Content ◽  
Artocarpus Heterophyllus ◽  
Phenol Group ◽  
The People ◽  
Leaf Powder

The leaves of jackfruit (Artocarpus heterophyllus), cempedak (Artocarpus integer), and tarap (Artocarpus odoratissimus) are used empirically by the people of Desa Pengaron, Banjar Regency for treat antimalarials, antidiabetic, abdominal pain, and cancer. The secondary metabolites of the phenol group are responsible for various plant activities. This study aims to determine of compounds and total phenol content in leaves of jackfruit (A. heterophyllus), cempedak (A. integer), and tarap (A. odoratissimus). The leaf samples were oven-dried, then the leaf powder was extracted using ethanol. Phytochemical screening was carried out on the extract using reagents, and phenol content was determined using a spectrophotometer UV-Vis. The results showed that the extracts of leaves jackfruit (A. heterophyllus), cempedak (A. integer), and tarap (A. odoratissimus) contained phenol, flavonoids, and tannins. Total phenol content of leaves A. heterophyllus, A. integer and A. odoratissimus were 13,174 ± 1,378 mgGAE / gram extract, 37,204 ± 2,202 mgGAE / gram extract, and 35,886 ± 0.890 mgGAE / gram extract.

scholarly journals Antioxidant activity and total phenolic content in Caulerpa filiformis (Chlorophyta) from Sechura Bay and Paracas Bay, Peru

2020 ◽  
Vol 27 (1) ◽  
pp. 061-066 ◽  
Author(s):  
Joyce Mamani ◽  
Jorge Chávez ◽  
Eder Apumayta ◽  
Patricia Gil-Kodaka
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Methanolic Extract ◽  
Total Phenol ◽  
North Coast ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Phytochemical Screening ◽  
Phenol Content ◽  
The North

In Peru, Caulerpa filiformis is a marine algae listed as an invasive species. For years, its distribution has been considered to be in the north coast (Isla Lobos de Afuera and Piura) until a recent report of its distribution in the central coast (Ancash, Lima, and Ica). The present investigation aims to determine the main groups of secondary metabolites, total phenol content, and antioxidant activity of the methanolic extract of C. filiformis from Sechura Bay (Piura) and Paracas Bay (Ica). The main chemical groups were determined through phytochemical screening, the content of phenols by the Folin–Ciocalteu method, and antioxidant activity by the ABTS method (2,2-azinobis-[3-ethylbenzothiazoline-6-sulfonic acid]) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH). The phytochemical screening of the methanolic extract of C. filiformis from Sechura Bay and Paracas Bay revealed the presence of carbohydrates, polyphenols, tannins, flavonoids, lipids, alkaloids, steroids, and triterpenes for both extracts. The total phenol content of the extract of C. filiformis from Sechura Bay (39.31 ± 0.39 mg of AGE/g extract) was significantly higher (p < 0.05) than that from Paracas Bay (18.78 ± 0.31 mg of AGE/g extract). In the ABTS and DPPH assays, the antioxidant capacity of the Sechura C. filiformis extract (IC50 = 3.49 ± 0.01 and 2.18 ± 0.02 mg/mL) was significantly higher (p < 0.05) than that of the Paracas C. filiformis extract (IC50 = 6.41 ± 0.02 and 2.42 ± 0.04 mg /mL). These findings suggest that the methanolic extract of C. filiformis is a source of secondary metabolites with an antioxidant potential.

scholarly journals Phytochemical Characterization, In Vitro Antioxidant, Cytotoxic, and Antibacterial Effects of Aristolochia longa L.,

2020 ◽  
Vol 11 (1) ◽  
pp. 8129-8140
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Colorimetric Method ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phytochemical Screening ◽  
Phenol Content

The aim of this study was devoted, in the one hand, to determine a preliminary phytochemical screening of methanolic and aqueous root extracts of Aristolochia longa L. In the other hand, polyphenols and flavonoids contents, in vitro antioxidant, antitumor, and antibacterial activities of the organic extract of A. longa were determined. Phytochemical screening was carried out using standard methods of precipitation and coloration reactions. The total phenol content and flavonoid contents were determined using the Folin-Ciocalteu method and Aluminum chloride colorimetric method, respectively. Antioxidant activity was studied using DPPH assay. The cytotoxic activity of extracts was evaluated against three cancerous cell lines using MTT assay, and antibacterial activity was tested against Escherichia coli, Pseudomonas aeruginosa, Rhodococcus equi, and Staphylococcus aureus using the agar well diffusion assay. Preliminary phytochemical screening for aqueous and ethanolic extracts revealed the presence of starch, tannins, flavonoids, coumarins, and anthocyanins. The total phenol content of A. longa extracts showed that the methanol extract has the highest polyphenol and flavonoid concentrations with 101.4 mg GAE (Gallic acid equivalent)/g and 54.21 mg QE (Quercetin equivalent)/g extract, respectively. The methanolic extract also exhibited the highest antioxidant capacity of DPPH (IC50 = 1.32 mg/mL). While hexane extract exhibited both a potent inhibitory effect on VERO cell growth with IC50 = 15.125 μg/mL and a total inhibitory effect on Staphylococcus aureus with inhibition zone 8.5 mm. The high content of polyphenols in A. longa explains their antioxidant, antitumor, and antibacterial activity. Thus, this plant could be a significant source of natural compounds in preventing the development of cancer.

Evaluation of virgin olive oil bitterness by total phenol content analysis

2007 ◽  
Vol 109 (3) ◽  
pp. 193-197 ◽  
Author(s):  
Gabriel Beltrán ◽  
María T. Ruano ◽  
Antonio Jiménez ◽  
Marino Uceda ◽  
María P. Aguilera
Keyword(s):  
Content Analysis ◽  
Olive Oil ◽  
Virgin Olive Oil ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content

scholarly journals Total Phenol Content and Antimicrobial Activity of Lingonberry (Vaccinium vitis-idaea L.) from Several Areas in the Eastern Carpathians

2017 ◽  
Vol 9 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Éva LASLO ◽  
Zoltán Attila KÖBÖLKUTI
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Effect ◽  
Bioactive Compound ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Eastern Carpathians ◽  
Spoilage Bacteria ◽  
New Perspective ◽  
Similar Growth

This study evaluated the antimicrobial activity and the total phenol content of Vaccinium vitis idaea L. berry fruit from five different localities with distinct growth sites in the Eastern Carpathians. The antibacterial effect of lingonberry was studied on nine selected Gram-positive and negative, foodborne, illness causing and spoilage bacteria. The total phenol content was estimated by the Folin-Ciocalteu method. The present results showed stronger antibacterial effect of lingonberry on Gram-negative bacteria, especially on Pseudomonas fluorescens and Pseudomonas aeruginosa. The total phenol content varied between 3.72 and 2.1 mM GAE/ml. As data suggested, Vaccinium vitis-idaea fruits originating from different geographic regions and environment, differ from each other in terms of bioactive compound quantity and activity. In the selection of new perspective cultivars of lingonberry, the geographical origin of fruits must be considered. Two Step Cluster analysis detected relatively well supported relationship between samples provided from similar growth sites. Correlation analysis showed no correlation between altitude, phenol content and antimicrobial activity.

scholarly journals Prediction of Total Phenolic Content in Extracts of Prunella Species from HPLC Profiles by Multivariate Calibration

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Saliha Sahin ◽  
Esra Isik ◽  
Cevdet Demir
Keyword(s):  
High Performance ◽  
Multivariate Calibration ◽  
Principal Component Regression ◽  
Principal Component ◽  
Total Phenol ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Calibration Methods ◽  
Hplc Profiles

The multivariate calibration methods—principal component regression (PCR) and partial least squares (PLSs)—were employed for the prediction of total phenol contents of four Prunella species. High performance liquid chromatography (HPLC) and spectrophotometric approaches were used to determine the total phenol content of the Prunella samples. Several preprocessing techniques such as smoothing, normalization, and column centering were employed to extract the chemically relevant information from the data after alignment with correlation optimized warping (COW). The importance of the preprocessing was investigated by calculating the root mean square error (RMSE) for the calibration set of the total phenol content of Prunella samples. The models developed based on the preprocessed data were able to predict the total phenol content with a precision comparable to that of the reference of the Folin-Ciocalteu method. PLS model seems preferable, because of its predictive and describing abilities and good interpretability of the contribution of compounds to the total phenol content. Multivariate calibration methods were constructed to model the total phenol content of the Prunella samples from the HPLC profiles and indicate peaks responsible for the total phenol content successfully.

scholarly journals Strawberry jams: influence of different pectins on colour and textural properties

2009 ◽  
Vol 27 (No. 1) ◽  
pp. 20-28 ◽  
Author(s):  
M. Kopjar ◽  
V. Piližota ◽  
N.N. Tiban ◽  
D. Šubarić ◽  
J. Babić ◽  
...  
Keyword(s):  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Textural Properties ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Anthocyanin Content ◽  
Phenol Content ◽  
Quality Properties ◽  
Texture Parameters ◽  
Important Quality

Colour and texture are very important quality properties of all foods. In this work, the influence was investigated of different types of pectin on colour and textural properties in strawberry jams and low-calorie strawberry jams containing fructose and aspartame or fructose syrup and aspartame. The highest anthocyanin content and total phenol content were detected in strawberry jam samples prepared with low methoxy amidated pectin. During storage, after 4 and 6 weeks at both storage temperatures, room temperature and 4°C, anthocyanin content and total phenol content decreased. Also, free radical scavenging activity decreased during storage. As far as the texture parameters are concerned, namely firmness, consistency and cohesiveness, the highest values were found in strawberry jam samples prepared with high methoxyl pectin.

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