scholarly journals Isolation and identification of proteolytic, amylolytic, lipolytic, and chitinolytic bacteria from shrimp waste

2021 ◽  
Vol 28 (1) ◽  
pp. e18353
Author(s):  
Susana Sirvas Cornejo ◽  
Vanesa Buleje ◽  
Licia Salvatierra ◽  
Michael L. Jaramillo
Keyword(s):  
Waste Treatment ◽  
Industrial Applications ◽  
Chemical Processes ◽  
Shrimp Waste ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Microbial Enzymes ◽  
Chitinolytic Bacteria ◽  
By Products ◽  
Potential Use

Bacteria and microbial enzymes are biocatalysts and can be used as an alternative to industrial chemical processes. The present study focused on isolating and identifying bacterial strains from shrimp waste, that produce amylases, lipases, proteases and chitinases with potential use on shrimp waste treatment. Thirty-two bacterial strains were isolated, phenotypically characterized, and identified by the API system and the molecular analysis of the 16S rDNA. It was found that 28.13% of the isolated bacterial strains had amylolytic capacity, 87.50% lipolytic, 96.88% proteolytic and 28.13% chitinolytic capacity on agar plates with specific substrates. The genera Bacillus, Burkholderia, Ochrobactrum, Vibrio, Pseudomonas and Shewanella were identified. Bacteria with enzymatic capacities isolated in the present study, could be used to obtain by-products from shrimp waste as well as other industrial applications.

scholarly journals Isolation and characterization of bacterial strains with a hydrolytic profile with potential use in bioconversion of agroindustial by-products and waste

2013 ◽  
Vol 33 (2) ◽  
pp. 295-303 ◽  
Author(s):  
Cintia Anabela Mazzucotelli ◽  
Alejandra Graciela Ponce ◽  
Catalina Elena Kotlar ◽  
María del Rosario Moreira
Keyword(s):  
Bacterial Strains ◽  
Isolation And Characterization ◽  
By Products ◽  
Potential Use

scholarly journals Influence of indigenous mixotrophic bacteria on pyrite surface chemistry: Implications for bioflotation

2020 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Edy Sanwani ◽  
Nuslia Bayangkara Lamandhi ◽  
Halimatul Husni ◽  
Siti Khodijah Chaerun ◽  
Widi Astuti ◽  
...  
Keyword(s):  
Surface Properties ◽  
Low Cost ◽  
Chemical Processes ◽  
Pyrite Surface ◽  
Bacterial Strains ◽  
Chemical Reagents ◽  
Experimental Systems ◽  
Index Value ◽  
Physical And Chemical ◽  
Potential Use

Given the low-cost and eco-friendly method, biotechnology has been widely utilized in industries as an alternative for physical and chemical processes, including in the biomining process (e.g., bioflotation and biobeneficiation). However, the use of biochemical reagent, which is selective for certain minerals, has not been well studied. This research was aimed to investigate the potential use of biosurfactant-producing mixotrophic bacteria as an alternative to chemical reagents during bioflotation and biobeneficiation process. Thirteen bacterial strains were investigated for their ability to produce biosurfactants and their effects on the surface properties of pyrite minerals. Bacteria-pyrite interaction experimental results showed that pyrite surface properties became more hydrophilic in the experimental systems inoculated with bacteria adapted with pyrite for 48 h than that without bacterial adaptation to pyrite, which was evidenced by the decrease in the contact angle of pyrite minerals by up to 50%. This evidence was also confirmed by the highest emulsifying index value (51.6%) attained during the bacteria-pyrite interaction. Hence, these bacteria can potentially be applied to selective flotation as pyrite depressants.

Isolation and identification of IAA producing endosymbiotic bacteria from Gracillaria corticata (J. Agardh)

2016 ◽  
Vol 5 (12) ◽  
pp. 5179
Author(s):  
Ilahi Shaik* ◽  
P. Janakiram ◽  
Sujatha L. ◽  
Sushma Chandra
Keyword(s):  
Acetic Acid ◽  
Culture Filtrate ◽  
Indole Acetic Acid ◽  
Shoot Growth ◽  
High Salinity ◽  
Saline Soils ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Endosymbiotic Bacteria ◽  
Root And Shoot Growth

Indole acetic acid is a natural phytohormone which influence the root and shoot growth of the plants. Six (GM1-GM6) endosymbiotic bacteria are isolated from Gracilaria corticata and screened for the production of IAA out of six, three bacterial strains GM3, GM5 and GM6 produced significant amount of IAA 102.4 µg/ml 89.40 µg/ml 109.43 µg/ml respectively. Presence of IAA in culture filtrate of the above strains is further analyzed and confirmed by TLC. As these bacterial strains, able to tolerate the high salinity these can be effectively used as PGR to increase the crop yield in saline soils.

scholarly journals Antimicrobial Activity and Chemical Characterization of a Non-Polar Extract of Saffron Stamens in Food Matrix

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 703
Author(s):  
Severino Zara ◽  
Giacomo L. Petretto ◽  
Alberto Mannu ◽  
Giacomo Zara ◽  
Marilena Budroni ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Fatty Acid Content ◽  
Chemical Characterization ◽  
Antimicrobial Activities ◽  
Added Value ◽  
Gas Chromatography Mass Spectrometry ◽  
Food Matrix ◽  
Bacterial Strains ◽  
By Products

The production of saffron spice generates large quantities of plant by-products: over 90% of the plant material collected is discarded, and a consideration fraction of this waste is plant stamens. This work investigated the chemical composition and the antimicrobial activities of the non-polar fraction extracted from four different saffron flower stamens. The chemical composition of ethereal extracts of the saffron stamens was qualitatively assessed by means of gas–chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance (NMR) analyses. These analyses revealed ethereal extracts to possess a high polyunsaturated fatty acid content. In vitro antibacterial activity of stamen extracts showed no large differences between Gram-positive and Gram-negative bacteria in terms of minimal inhibitory concentration (MIC). In food matrix microbial analysis of the bacterial strains belonging to the main foodborne pathogen species, including Staphylococcus aureus DSM 20231, Escherichia coli DSM 30083, and Listeria monocytogenes DSM 20600, using low-fat UHT milk, revealed a statistically significant reduction in the number of cells (particularly for E. coli and S. aureus with a complete elimination of the population of the two target bacteria following incubation in diethyl ether extracts of saffron stamen (DES) at high concentrations tested, both at 37 °C and 6 °C (for 48 h and 7 days, respectively). A synergic effect was observed when the pathogens were incubated at 6 °C with DES. This work shows these by-products to be excellent sources of bioactive compounds, which could be exploited in high-added-value products, such as food, cosmetics, and drugs.

Isolation and Identification of Bacterial Strains from Decomposing Hazelnut Husk

2015 ◽  
Vol 23 (3) ◽  
pp. 174-184 ◽  
Author(s):  
Rıdvan Kızılkaya ◽  
Nevzat Sahin ◽  
Demet Tatar ◽  
Aysel Veyisoglu ◽  
Tayfun Askın ◽  
...  
Keyword(s):  
Bacterial Strains ◽  
Isolation And Identification ◽  
Hazelnut Husk

Microbial enzymes and potential use in algal polysaccharide modifications

2019 ◽  
pp. 375-394
Author(s):  
Daniela de Borba Gurpilhares ◽  
Lara Durães Sette ◽  
Adalberto Pessoa
Keyword(s):  
Microbial Enzymes ◽  
Potential Use

scholarly journals Bioprocessing of Shrimp Waste Using Novel Industrial By-Products: Effects on Nutrients and Lipophilic Antioxidants

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 312
Author(s):  
Luis Angel Cabanillas-Bojórquez ◽  
Erick Paul Gutiérrez-Grijalva ◽  
Ramón Ignacio Castillo-López ◽  
Laura Aracely Contreras-Angulo ◽  
Miguel Angel Angulo-Escalante ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Rich Source ◽  
Added Value ◽  
The Novel ◽  
Shrimp Waste ◽  
Acid Fermentation ◽  
Trolox Equivalent ◽  
By Products

The production of marine foods is on the rise, and shrimp is one of the most widely consumed. As a result, a considerable amount of shrimp waste is generated, becoming a hazardous problem. Shrimp waste is a rich source of added-value components such as proteins, lipids, chitin, minerals, and carotenoids; however, new bioprocesses are needed to obtain these components. This work aimed to characterize the chemical and nutraceutical constituents from the liquor of shrimp waste recovered during a lactic acid fermentation process using the novel substrate sources whey and molasses. Our results showed that the lyophilized liquor is a rich source of proteins (25.40 ± 0.67%), carbohydrates (38.92 ± 0.19%), minerals (calcium and potassium), saturated fatty acids (palmitic, stearic, myristic and lauric acids), unsaturated fatty acids (oleic acid, linoleic, and palmitoleic acids), and astaxanthin (0.50 ± 0.02 µg astaxanthin/g). Moreover, fermentation is a bioprocess that allowed us to obtain antioxidants such as carotenoids with an antioxidant capacity of 154.43 ± 4.73 µM Trolox equivalent/g evaluated by the ABTS method. Our study showed that liquor from shrimp waste fermentation could be a source of nutraceutical constituents with pharmaceutical applications. However, further studies are needed to separate these added-value components from the liquor matrix.

scholarly journals Screening of Effective Media for Black Soldier Fly Larvae Used in Food Waste Treatment

2020 ◽  
Vol 42 (10) ◽  
pp. 463-471
Author(s):  
Chul-Hwan Kim ◽  
Kwanyoung Ko ◽  
Jongkeun Lee ◽  
Haegeun Chung
Keyword(s):  
Food Waste ◽  
Wheat Bran ◽  
Waste Treatment ◽  
Treatment Method ◽  
Treatment Technology ◽  
Black Soldier Fly ◽  
Black Soldier Fly Larvae ◽  
Fly Larvae ◽  
By Products ◽  
Bean Sprouts

Objectives : Black soldier fly larvae (BSFL) are organisms that effectively decompose various types of organic waste including food waste, and food waste treatment using BSFL is attracting attention as a sustainable waste treatment method. However, food waste discharged from Korea has a wide variety of properties, and its high salt concentration limits its treatment by BSFL. Therefore, to increase the efficiency of food waste treatment using BSFL, it is necessary to increase the quality of food waste as a production medium for BSFL. In this study, the ratio of protein and fat was adjusted by adding bean sprouts and wheat brans to food wastes treated at high temperature under vacuum, and whether such medium is suitable for rearing BSFL was investigated.Methods : To improve the medium, the ratio of protein and fat was adjusted to approximately 2:1 by adding bean sprouts and bran residue to food waste. Subsequently, the growth and development rate of BSFL reared on chicken feed, food waste, food waste + bean sprouts, food waste + wheat bran were measured. Also, the decomposition rate of each medium was analyzed.Results and Discussion : The growth rate of BSFL grown on food waste + wheat bran medium was similar to that of BSFL reared on chicken feed. The speed of development at day 7 was also the fastest for BSFL reared with food waste + wheat bran medium and chicken feed. These results suggest that the mixed medium to which wheat bran has been added to food waste has the potential to be used as a commercial medium for BSFL production. The survival rate of BSFL was 89% or higher in all media.Conclusions : When food waste was used alone, BSFL development was poor compared to that in media combined with agricultural by-products such as bean sprouts and wheat bran. Therefore, to use food waste as a rearing medium of BSFL, it is necessary to adjust the ratio of protein and fat by adding various agricultural by-products and reduce salinity. For the improvement of food waste treatment technology using BSFL, mass rearing of useful insects such as BSFL, and promotion of the use of agricultural by-products, additional research is needed to optimize the composition of rearing medium based on food waste.

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