scholarly journals Gallic Acid Content and Antioxidant Activity of Pomegranate Peel Ethanol Extract

2020 ◽  
Vol 52 (4) ◽  
Author(s):  
Diah Dhianawaty ◽  
Latifah Rahman Nurfazriah ◽  
Andri Rezano
Keyword(s):  
Cell Proliferation ◽  
Antioxidant Activity ◽  
Gallic Acid ◽  
Acid Content ◽  
High Performance ◽  
Ethanol Extract ◽  
Antitumor Effects ◽  
Pomegranate Peel ◽  
Chromatography Method ◽  
Pomegranate Peel Extract

Ethanol extract of Pomegranate peel is proven to have the ability to inhibit the proliferation of cervical cancer HeLa cells. It was reported that this extract contains ellagic acid, gallic acid, and punicalagin which are identified as major effectors of antitumor activity. Gallic acid has been demonstrated to have an antioxidant activity that can influence cancer cell proliferation. The aim of the study was to identify the gallic acid content in pomegranate peel ethanol extract and to measure its antioxidant. This study was an experimental study performed at the Biochemistry and Molecular Biology Laboratory and Central Laboratory of Universitas Padjadjaran Bandung, Indonesia during September-December 2019. Gallic acid concentration was measured by using a high-performance liquid chromatography method (HPLC) and the antioxidant activity was measured by using 1,1-diphenyl-2-picrylhydrazyl (DPPH). The extract contained 616.97 mg/kg of gallic acid with an antioxidant activity of IC50 6.1 µg/mL. In conclusion, gallic acid has cytotoxic and antitumor effects from its ability to modulate antioxidant/pro-oxidant balance and inhibit reactive oxygen species (ROS). Therefore, both gallic acid content and antioxidant activity parameters can be used as markers in pomegranate peel extract which shows activities that inhibit HeLa cell proliferation.

scholarly journals In vitro anti-adenoviral activities of ethanol extract, fractions, and main phenolic compounds of pomegranate (Punica granatum L.) peel

2020 ◽  
Vol 28 ◽  
pp. 204020662091657
Author(s):  
Ali Karimi ◽  
Mohammad-Taghi Moradi ◽  
Mohammad Rabiei ◽  
Somayeh Alidadi
Keyword(s):  
Phenolic Compounds ◽  
Gallic Acid ◽  
Virus Replication ◽  
Ethanol Extract ◽  
Inhibitory Effect ◽  
Pomegranate Peel ◽  
Butanol Fraction ◽  
Pomegranate Peel Extract ◽  
Peel Extract

Background Adenovirus causes a number of diseases in human, and can cause serious infection in severely immunosuppressed individuals. Despite the seriousness of adenovirus infection, there is no definitely approved anti-adenoviral therapy. Many studies have shown that compounds derived from medicinal plants have antiviral activity. Therefore, this study evaluated in vitro anti-adenoviral activity of ethanol extract, fractions, and main phenolic compounds of pomegranate peel. Methods The ethanol extract of pomegranate peel was prepared with maceration method and fractionated by consecutive liquid/liquid partition. The cytotoxic and anti-adenovirus activities of the extract, fractions, and main phenolic compounds (ellagic acid, punicalagin and gallic acid) were evaluated on Hep-2 cell line using MTT assay. Inhibitory effect on adsorption and post-adsorption phases of the virus replication cycle was also evaluated. Results Pomegranate peel extract had a desirable effect against adenovirus with IC50 of 5.77 µg/mL and selectivity index of 49.9. Among the fractions and compounds, the n-butanol fraction and gallic acid had the highest anti-adenoviral activity with IC50 of 2.16 µg/mL and 4.67 µM and selectivity indices of 122.5 and 10.5, respectively. The crude extract, n-butanol fraction and gallic acid inhibited the virus replication in post-adsorption phase ( p <  0.01). Conclusion Pomegranate peel extract, especially its n-butanol fraction, could serve as a new promising anti-adenovirus agent due to high inhibitory effect against adenovirus replication. The effect of the n-butanol fraction may be related to the synergistic effect or other compounds of this fraction. Further understanding of the bioassay guided isolation of natural compounds of this fraction seems essential.

scholarly journals Effect of Chitosan–Pullulan Composite Edible Coating Functionalized with Pomegranate Peel Extract on the Shelf Life of Mango (Mangifera indica)

Coatings ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 764
Author(s):  
Nishant Kumar ◽  
Pratibha ◽  
Neeraj ◽  
Anka Trajkovska Petkoska ◽  
Sawsan Ali AL-Hilifi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Shelf Life ◽  
Mangifera Indica ◽  
Storage Period ◽  
Edible Coating ◽  
Postharvest Quality ◽  
Pomegranate Peel ◽  
Preservation Method ◽  
Pomegranate Peel Extract ◽  
Peel Extract

The polysaccharide based composite biomaterial (coating) used in preserving fruits and vegetables during storage is attracting increased attention as it is biodegradable material that prolongs shelf life. In the present investigation, chitosan–pullulan (50:50) composite edible coating was prepared with pomegranate peel extract (0.02 g/mL) as an active antioxidant agent. The effect of treatment with pomegranate peel extract enriched chitosan–pullulan composite edible coating on the shelf life of mango fruits during 18 days of storage period at room (23 °C) and cold (4 °C) temperature was evaluated. Results of the present study demonstrated that the application of chitosan–pullulan composite edible coating significantly (p ≤  0.05) influences the storage life of mango fruits at both storage temperatures. The chitosan–pullulan composite edible coating reduced the physiological loss in weight (PLW), and maintained total soluble solids (TSS), acidity and pH of coated mango fruits as compared to the control. In addition, fruit sensory quality such as freshness, color, taste and texture were also retained by the treatment. Furthermore, sustained firmness, phenolic content and antioxidant activity confirmed the effectiveness of the pomegranate peel extract enriched chitosan–pullulan composite edible coating on mango fruits. The phenolic, flavonoid and antioxidant activity of coated fruits were retained by pomegranate peel rich edible coating. Therefore, the chitosan–pullulan (50:50) combination with pomegranate peel extract can be used as an alternative preservation method to prolong the shelf life of mango fruits at room and cold storage conditions. However, more in-depth studies are required at farm and transit level without affecting the postharvest quality of mango fruits, providing more revenue for farmers and minimizing postharvest losses.

scholarly journals Application of the thin-layer chromatography method for analysis of plant antioxidant activity

Food systems ◽  
2021 ◽  
Vol 4 (1) ◽  
pp. 26-30
Author(s):  
N. V. Kupaeva ◽  
E. R. Vasilevskaya ◽  
L. V. Fedulova ◽  
E. A. Kotenkova
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Chromatography Method ◽  
Physicochemical Methods ◽  
Ethanol Extracts ◽  
Layer Chromatography ◽  
Active Substances

Plants are a rich source of effective non-toxic biologically active substances. Various physicochemical methods of analysis are used for evaluation of plant antioxidant activity. Composition of ethanol extracts of red, yellow and white onion husks, dried rosemary, basil, and chaga were studied by high performance thin layer chromatography (HPTLC) method. The antioxidant activity of the obtained fractions on a chromatographic plate was assessed by subsequent DPPH screening. The extracts red and yellow onion husk and rosemary demonstrated the highest antioxidant activity, variability of the qualitative composition and similarity of antioxidant profiles, while extract of white onion husks did not contain any antioxidant classes. Intensive spots with Rf of 0.13-0.97 were observed along the whole chromatogram track corresponding to red onion husks. It was also found that all tested extract, excepting white onion husk and chaga, contained spots with varying degrees of intensity in the Rf range of 0.96-0.98, which corresponded quercetin Rf value.

scholarly journals Five-Golden-Flowers Tea: Green Extraction and Hepatoprotective Effect against Oxidative Damage

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2216 ◽  
Author(s):  
Cai-Ning Zhao ◽  
Guo-Yi Tang ◽  
Qing Liu ◽  
Xiao-Yu Xu ◽  
Shi-Yu Cao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
High Performance ◽  
Antioxidant Properties ◽  
Dry Weight ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Herbal Tea ◽  
Antioxidant Power

The consumption of herbal teas has become popular in recent years due to their attractive flavors and outstanding antioxidant properties. The Five-Golden-Flowers tea is a herbal tea consisting of five famous edible flowers. The effects of microwave-assisted extraction parameters on the antioxidant activity of Five-Golden-Flowers tea were studied by single-factor experiments, and further investigated using response surface methodology. Under the optimal parameters (53.04 mL/g of solvent/material ratio, 65.52 °C, 30.89 min, and 500 W), the ferric-reducing antioxidant power, Trolox equivalent antioxidant capacity, and total phenolic content of the herbal tea were 862.90 ± 2.44 µmol Fe2+/g dry weight (DW), 474.37 ± 1.92 µmol Trolox/g DW, and 65.50 ± 1.26 mg gallic acid equivalent (GAE)/g DW, respectively. The in vivo antioxidant activity of the herbal tea was evaluated on alcohol-induced acute liver injury in mice. The herbal tea significantly decreased the levels of aspartate aminotransferase, total bilirubin, and malonaldehyde at different doses (200, 400, and 800 mg/kg); improved the levels of liver index, serum triacylglycerol, and catalase at dose of 800 mg/kg. These results indicated its role in alleviating hepatic oxidative injury. Besides, rutin, chlorogenic acid, epicatechin, gallic acid, and p-coumaric acid were identified and quantified by high performance liquid chromatography (HPLC), which could contribute to the antioxidant activity of the herbal tea.

scholarly journals Development of High Performance Thin Layer Chromatography Method for Quantitation of Gallic Acid in Normacid Syrup: A Poly Herbal Formulation

2021 ◽  
Vol 11 (1-s) ◽  
pp. 49-52
Author(s):  
Kinjal H Shah ◽  
Rajeshree Makwana
Keyword(s):  
Gallic Acid ◽  
High Performance ◽  
Control Method ◽  
Quantitative Estimation ◽  
Chromatography Method ◽  
Herbal Formulation ◽  
Quality Control Method ◽  
Layer Chromatography ◽  
Hptlc Method ◽  
Nanogram Level

Normacid syrup consisiting of 17 ingredients, used in the treatment of hyperacidity and gastritis. Gallic acid is one of the phytoconstituent present in Normacid syrup- a poly herbal formulation. In the present study an attempt has been made to develop a HPTLC method for quantitative estimation of gallic acid in dried fruits used in formulations. This HPTLC method was found to be reproducible, accurate, precise and detect gallic acid at nanogram level. The developed HPTLC method would be an important tool in the quality control method of poly herbal formulation.  Keywords: HPTLC, Polyherbal, Gallic acid, Gastritis

scholarly journals Antioxidant (gallic acid and quercetin) profile of Sumatran wild mangoes (Mangifera spp.): a potential source for antidegenerative medicine

F1000Research ◽  
2020 ◽  
Vol 9 ◽  
pp. 220
Author(s):  
Fitmawati Fitmawati ◽  
Esi Resida ◽  
Sri Nur Kholifah ◽  
Rodesia Mustika Roza ◽  
Muhammad Almurdani ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
High Performance ◽  
Dry Weight ◽  
Hplc Method ◽  
Flavonoid Compound ◽  
The Public ◽  
New Findings ◽  
Phenolic Analysis

Background: New findings on the potential of wild mangoes from the island of Sumatra as a source of antioxidant helps their conservation effort as it introduces their useful compounds to the public. This study aims to analyze the antioxidant profile and quantification of gallic acid and quercetin content from leaves and bark of Sumatran wild mangoes. Exploration and analysis of phytochemical constituents from 11 Sumatran wild mangoes was performed. Methods: Antioxidant activity of wild mangoes was analysed with 1,1- diphenyl-2-picryl hydroxyl (DPPH), and determination of quercetin and gallic acid content was performed by high performance liquid chromatography (HPLC) method. Total flavonoid and phenolic analysis was also performed. Curve fitting analysis used a linear regression approach. Results: The highest level of antioxidant activity, phenolic compound and flavonoid compound was found in the leaves and bark of Mangifera sp1. (MBS), the bark of M. foetida3 (var. batu) and leaves of M. torquenda, and the bark and leaves of M. sumatrana, respectively. The content of gallic acid in leaves ranged from 5.2270-35.4763 mg/g dry weight. Quercetin content of wild mangoes leaves ranged from 0.76 to 1.47 mg/g dry weight with the lowest value in M. foetida2 (var. manis) and the highest in M. laurina. Conclusion: The results obtained are expected to be useful in supporting the development of antidegenerative drugs from natural ingredients that have potential as immunomodulatory agents.

scholarly journals QUANTIFICATION OF PHYTOCHEMICAL CONSTITUENTS AND IN VITRO ANTIOXIDANT ACTIVITY IN THE LEAVES OFCITRUS MEDICA

2017 ◽  
pp. 119-123
Author(s):  
Mayavatis Patil
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Gallic Acid ◽  
Ethanol Extract ◽  
Maximum Level ◽  
Maximum Activity ◽  
Total Phenolic ◽  
Alkaloid Contents ◽  
In Vitro Antioxidant Activity

Objective: In the present investigation, phytochemical assay and in vitro antioxidant activity of ethanol (70%), methanol, ethyl acetate and hexane extract of Citrus medica leaves were carried out.Methods: The quantification of total phenolic and alkaloid contents were estimated by taking gallic acid and atropine as standard. In–vitro antioxidant activity of extracts was evaluated by using different free radicals (DPPH, superoxide and free radicals).Results: Ethanol extract of leaves have more phenolic and alkaloid contents than other extracts. The selected plant extract was produced concentration dependent percentage inhibition of different free radicals and produced maximum activity at a concentration of 1000µgm/ml, and there after percentage inhibition was raised gradually to its maximum level with higher concentrations. HPLC analysis revealed the presence of Gallic acid, Catechein, Rutin, Chlorgenic acid, Queracetine and some unknown components which need to be e identified.Conclusion: Among the four extracts, ethanol extract of C. medica showed good antioxidant activity. 

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