scholarly journals AN EFFECTIVE WAY TO CARRY OUT MASS IN VITRO PROPAGATION OF POTENTILLA ALBA L.

2016 ◽  
Vol 6 (01) ◽  
pp. 433-444
Author(s):  
L. I. Tikhomirova ◽  
A. A. Kechaykin ◽  
A. I. Shmakov ◽  
O. V. Aleksandrova
Keyword(s):  
Culture Media ◽  
Raw Materials ◽  
Effective Means ◽  
Growth Conditions ◽  
Test Tube ◽  
Rare And Endangered Species ◽  
In The Wild ◽  
Rare And Endangered ◽  
The Republic

<p>Despite the plant’s extensive area of distribution, <em>Potentilla alba </em>L.<em> </em>natural resources are scarce and cannot meet the modern needs of the pharmaceutical industry. Because of the mass preparation of medical raw materials by using <em>P. alba</em>, it entered into the list of rare and endangered species plants of the Red Data Book of the Republic of Belarus. This plant is not represented in the wild flora of Western Siberia, but there is a great need for developing a method for the mass propagation of <em>P. alba</em> using <em>in vitro</em> culture in order to obtain a high-quality planting material. At the explant stage, the technique of the <em>P. alba</em> introduction into <em>in vitro</em> culture is developed. This paper reveals the morphogenetic features of the development of <em>P. alba</em> explants of different types and the regenerative capacity of the tissue culture. At the micropropagation stage, the optimum culture media and the growth conditions for the regenerated plants are selected. At the stage of test-tube plants rooting and transferring them into <em>ex vitro</em> conditions, the most effective means of adaptation to non-sterile conditions in hydroponics are proposed.</p>

scholarly journals In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
Yupi ISNAINI ◽  
Titien Ngatinem Praptosuwiryo
Keyword(s):  
Spore Germination ◽  
Culture Media ◽  
Basal Medium ◽  
Modern Medicine ◽  
Ex Situ ◽  
Naphthalene Acetic Acid ◽  
Gametophyte Development ◽  
In The Wild ◽  
Rare And Endangered

Abstract. Isnaini Y, Praptosuwiryo TNg. 2020. In vitro spore germination and early gametophyte development of Cibotium barometz (L.) J. Sm. in different media. Biodiversitas 21: 5373-5381. Cibotium barometz (L.) J. Sm. is known as the golden chicken fern and included in Appendix II of CITES. It is an important export commodity for traditional and modern medicine. Globally, populations of this species are under significant pressure due to overexploitation in the wild. In vitro culture is one of the technologies used for ex-situ propagation and conservation of rare and endangered ferns and lycophytes. This study’s objectives were: (i) to observe in vitro spore germination and early gametophyte development of C. barometz, and (ii) to determine the best culture medium for rapid spore germination and early development of the gametophytes. The sterilized spores were sown in half-strength Murashige & Skoog (½MS) basal medium supplemented with combinations of 6-Benzylaminopurine (BAP) and α-Naphthalene acetic acid (NAA). A factorial combination of four BAP concentrations (0, 2, 4, and 6 mg L-1) with four concentrations of NAA (0; 0.01; 0.03 and 0.05 mg L-1) created 16 treatments replicated in a Completely Randomized Design. Spore germination of C. barometz was observed to be Vittaria-type, and its prothallial development was Drynaria-type. Spore germination started 7-14 days after sowing. Young heart-shape gametophytes consisting of 110-240 cells were formed in 45-61 days after sowing. The two best spore culture media for rapid spore germination and development of C. barometz gametophytes were ½ MS with or without 2 mg L-1 BAP.

scholarly journals MICROCLONAL PROPAGATION OF KARELIAN BIRCH AND TRIPLOID ASPEN IN VITRO

ÈKOBIOTEH ◽  
2021 ◽  
Vol 4 (2) ◽  
pp. 101-106
Author(s):  
N.S. Anokhina ◽  
◽  
V.F. Konovalov ◽  
E.R. Khanova ◽  
◽  
...  
Keyword(s):  
Tree Species ◽  
Gene Pool ◽  
Raw Materials ◽  
High Quality ◽  
The Republic ◽  
Methodological Aspects ◽  
Karelian Birch

Methodological aspects of microclonal reproduction of valuable tree species – triploid aspen and Karelian birch, which are important for enriching the gene pool of the main forest-forming tree species in the Republic and obtaining high-quality wood raw materials in the form of ornamental and decorative wood, are proposed.

scholarly journals COMPARATIVE EVALUATION OF ANTIOXIDANT ACTIVITY OF EXTRACTS OF BERGENIA CRASSIFOLIA (L.) FITSCH LEAVES OF DIFFERENT TERMS OF VEGETATION

2020 ◽  
pp. 231-239
Author(s):  
Svetlana Vladimirovna Tsyrendorzhieva ◽  
Sesegma Dashiyevna Zhamsaranova
Keyword(s):  
Raw Materials ◽  
Antioxidant Properties ◽  
Total Content ◽  
Leaf Extracts ◽  
Green Leaves ◽  
Chelating Ability ◽  
The Republic ◽  
Bergenia Crassifolia ◽  
Maximum Content

New data on the chemical composition and antioxidant properties of aqueous leaf extracts of Bergenia crassifolia (L.) Fitsch of different periods of vegetation growing in the Republic of Buryatia were obtained. It is shown that this is one of the few plants whose leaves contain a significant amount of phenolic compounds. The maximum content of tannins was noted in the extract of red leaves of the incense – 2.42%. Depending on the phase of the collection of raw materials, the content of flavonoids in the water extracts of the leaves of the frangipani varied from 3.88 to 5.42%. A higher accumulation of flavonoids was observed in the extract of black leaves of badan, which is apparently associated with leaf fermentation, in which the cellular structure is destroyed, enzymes and polyphenols are released. The leader in determining the total content of anthocyanins, as well as for flavonoids, was the extract of black leaves of frankincense. The largest amount of arbutin was contained in the extract of green leaves of frankincense – 1.22%. The studied extracts had a high ability to absorb free radicals, however, the extract of green leaves of badan had the highest antiradical activity, had a minimum EC50 of 4 mg/cm3. The iron-chelating ability of the tested aqueous extracts of frankincense leaves of different periods of in vitro vegetation showed that the leaf extracts had weak iron-chelating activity.

scholarly journals PERTUMBUHAN KULTUR TUNAS AKSILAR KENTANG (Solanum tuberosum L.) DENGAN PENAMBAHAN SUPER FOSFAT DAN KNO3 PADA MEDIA AB MIX SECARA IN VITRO

2019 ◽  
Vol 20 (2) ◽  
pp. 71
Author(s):  
Hamami Alfasani Dewanto ◽  
Desi Saraswati ◽  
Oetami Dwi Hadjoeningtijas
Keyword(s):  
Culture Media ◽  
Raw Materials ◽  
Solanum Tuberosum L ◽  
Leaf Color ◽  
Tissue Culture Media ◽  
Randomized Design ◽  
Plantlet Growth ◽  
Number Of Leaves ◽  
Dark Green

Murashige&Skoog-based medium Potatoes are one commodity that has the potential to be developed as a resource in the context of food diversification, farmers' income riser, non fossil export commodities and raw materials for processing industry. The objective of this research was to find out the effect of SP-36 fertilizer, KNO3 fertilizer, as well as the interaction between the two fertilizers on the growth of potato nodal culture on AB Mix media in vitro. The results of this study are expected to provide economical potato tissue culture media development. This research used factorial complete randomized design. The treatment were SP-36 concentration: 0 ppm; 50 ppm; 100 ppm; and 200 ppm, in combination with KNO3 concentration: 0 ppm; 100 ppm; 200 ppm; 400 ppm; and 600 ppm, The variables observed included number of leaves, leaf color, length of plantlets, fresh weight of plantlets and percentage of plantlets growth. Based on the results of the analysis of variance (ANOVA) F. Calculate < F. Table with the average success of plantlet growth between 87.5-100%. In addition, there are four types of leaf color produced, namely the color of yellowish green, pale green leaves, green, and dark green. Research showed that the interaction between SP-36 fertilizer and KNO3 fertilizer on AB Mix media had no significant effect on all observed variables.

scholarly journals Understanding Reproductive Aging in Wildlife to Improve Animal Conservation and Human Reproductive Health

2021 ◽  
Vol 9 ◽  
Author(s):  
Pierre Comizzoli ◽  
Mary Ann Ottinger
Keyword(s):  
Wild Species ◽  
Natural Habitat ◽  
Research Area ◽  
Laboratory Animals ◽  
Reproductive Aging ◽  
Multiple Traits ◽  
Rare And Endangered Species ◽  
In The Wild ◽  
Rare And Endangered ◽  
Human Reproductive

Similar to humans and laboratory animals, reproductive aging is observed in wild species-from small invertebrates to large mammals. Aging issues are also prevalent in rare and endangered species under human care as their life expectancy is longer than in the wild. The objectives of this review are to (1) present conserved as well as distinctive traits of reproductive aging in different wild animal species (2) highlight the value of comparative studies to address aging issues in conservation breeding as well as in human reproductive medicine, and (3) suggest next steps forward in that research area. From social insects to mega-vertebrates, reproductive aging studies as well as observations in the wild or in breeding centers often remain at the physiological or organismal scale (senescence) rather than at the germ cell level. Overall, multiple traits are conserved across very different species (depletion of the ovarian reserve or no decline in testicular functions), but unique features also exist (endless reproductive life or unaltered quality of germ cells). There is a broad consensus about the need to fill research gaps because many cellular and molecular processes during reproductive aging remain undescribed. More research in male aging is particularly needed across all species. Furthermore, studies on reproductive aging of target species in their natural habitat (sentinel species) are crucial to define more accurate reproductive indicators relevant to other species, including humans, sharing the same environment. Wild species can significantly contribute to our general knowledge of a crucial phenomenon and provide new approaches to extend the reproductive lifespan.

Preparation and Properties of Chitosan-Tranexamic Acid Salts

2019 ◽  
Vol 943 ◽  
pp. 129-134 ◽  
Author(s):  
Zhang Hu ◽  
Ya Qi Qin ◽  
Si Tong Lu ◽  
Chu Ru Huang ◽  
Ze Kun Chen
Keyword(s):  
Tranexamic Acid ◽  
Water Solubility ◽  
Raw Materials ◽  
Test Tube ◽  
X Ray Diffraction ◽  
Crystallinity Degree ◽  
Protonation Reaction ◽  
Coagulant Activity ◽  
Acid Salts

The solid chitosan-tranexamic acid salts were successfully prepared by the method of half-wet grinding with chitosan and tranexamic acid as raw materials. The physical properties including water solubility and stability of the prepared samples were tested, and their structures were characterized by Fourier transform infrared (FTIR) spectroscopy and X-ray diffraction (XRD). In addition, the in vitro pro-coagulant activity was evaluated by the test tube method. The results showed that the prepared chitosan-tranexamic acid salts could be dissolved in weak acidic, neutral and weak alkaline aqueous solutions, expanding the dissolution range. The characterization results indicated that the protonation reaction of the chitosan amino group was achieved without solvent and the crystallinity degree of chitosan-tranexamic acid salts decreased by comparison with chitosan. The hemostasis evaluation showed that chitosan-tranexamic acid salts with different mass ratios had pro-coagulant activities in vitro. In particular, the clotting time of chitosan acid salt with mass ratio of 1:2 was shorter than that of Yunnan Baiyao. This effort laid the foundation for the development of the promising chitosan-based hemostatic materials.

scholarly journals Induction of iron regulated proteins during normal growth of Neisseria meningitidis in a chemically defined medium

1994 ◽  
Vol 36 (4) ◽  
pp. 301-310 ◽  
Author(s):  
Maria Cristina de Cunto Brandileone ◽  
Rosemeire Cobo Zanella ◽  
Vera Simonsen Dias Vieira ◽  
Claudio Tavares Sacciii ◽  
Lucimar Gonçalves Milagres ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Culture Supernatant ◽  
Culture Media ◽  
Membrane Vesicles ◽  
Normal Growth ◽  
Growth Conditions ◽  
Defined Medium ◽  
Outer Membrane Vesicles ◽  
Iron Deficient

The expression of iron regulated proteins (IRPs) in vitro has been obtained in the past by adding iron chelators to the culture after bacterial growth, in the presence of an organic iron source. We have investigated aspects concerning full expression of the meningococcal IRPs during normal growth, in defined conditions using Catlin medium, Mueller Hinton and Tryptic Soy Broth (TSB). The expression of IRPs varied between different strains with respect to Ethylenediamine Di-ortho-Hidroxy-phenyl-acetic acid (EDDA) concentrations, and according to culture medium, and also between different lots of TSB. For each strain, a specific set of IRPs were expressed and higher EDDA concentrations, or addition of glucose, or use of different culture media did not resulted in a differential expression of IRPs. We were not able to grow N. meningitidis under normal growth conditions using Desferal. We looked for a good yield of outer membrane vesicles (OMVs) expressing IRPs in iron-deficient Catlin medium containing EDDA and Hemin. Culture for 32 h at 30ºC after growing for 16 h at 37ºC supported good bacterial growth. Bacterial lysis was noted after additional 24 h at 30ºC. Approximately 4 times more OMVs was recoverable from a culture supernatant after 24 h at 30ºC than from the cells after 16 h at 37ºC. The IRP were as well expressed in OMVs from culture supernatant obtained after 24 h at 30ºC as from the cells after 16 h at 37ºC.

In vitro propagation of Gentiana dinarica Beck.

2012 ◽  
Vol 7 (4) ◽  
pp. 690-697 ◽  
Author(s):  
Branka Vinterhalter ◽  
Dijana Milošević ◽  
Teodora Janković ◽  
Jelena Milojević ◽  
Dragan Vinterhalter
Keyword(s):  
Plant Growth Regulator ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Ms Medium ◽  
Root Initiation ◽  
Free Medium ◽  
Rare And Endangered Species ◽  
Rare And Endangered ◽  
Gentiana Dinarica

AbstractGentiana dinarica Beck, rare and endangered species of Balkan Dinaric alps, was in vitro propagated (micropropagated) from axillary buds of plants collected at Mt. Tara, Serbia. G. dinarica preferred MS to WPM medium, with optimal shoot multiplication on MS medium with 3% sucrose, 1.0 mg l−1 BA and 0.1 mg l−1 NAA. Rooting was not clearly separated from shoot multiplication since BA did not completely inhibit root initiation. Spontaneous rooting on plant growth regulator-free medium occurred in some 30% of shoot explants. Rooting was stimulated mostly by decreased mineral salt nutrition and a medium with 0.5 MS salts, 2% sucrose and 0.5–1.0 mg l−1 IBA was considered to be optimal for rooting. Rooted plantlets were successfully acclimated and further cultured in peat-based substrate.

scholarly journals Comparative Elimination of Begomoviruses in Cassava Meristems and Axillary Buds

2021 ◽  
pp. 1-9
Author(s):  
Adonise F. Valam Zango ◽  
Innocent Zinga ◽  
Régis Dimitri Longué Soupké ◽  
Simplice Prosper Yandia ◽  
Brice Toko Marabana ◽  
...  
Keyword(s):  
Plant Material ◽  
Central African Republic ◽  
Culture Media ◽  
Effective Means ◽  
Infected Plant ◽  
High Severity ◽  
Rate Of Emergence ◽  
The University ◽  
Infected Plant Material

Aim: The production of healthy cuttings from a local cassava cultivar for cassava mosaic control. Study design: The study was carried out at the In Vitro Culture Laboratory using the techniques of thermotherapy and culture of tissues and explants in a specific medium. Place and duration: The study was carried out at the Laboratory of Biological and Agronomic Sciences for Development at the University of Bangui, Central African Republic from December 2017 to June 2018. Methodology: A variety of cassava called six-month very susceptible to mosaic was used for this work. The cuttings used were infected by Cassava Mosaic Begomoviruses (CMBs) with high severity. It were subcultured in a room under the heat of 37 ° C to 40 ° C for two weeks. Explants and meristems were taken from the stems and the apices, respectively. These collected materials were treated and seeded on appropriate culture media. After the plants produced in vitro were acclimatized and the leaves were removed to check their phytosanitary state by the PCR technique. Results: The rate of emergence of the acclimatized plants and the expression of the disease on the microplants were evaluated. The results show that 75% of the weaned vitro plants recovered under acclimatization. In addition, the acclimatized plants left growing in the greenhouse for four months remained asymptomatic. Molecular analysis by PCR showed that begomoviruses were not detected on meristem samples unlike samples from stem fragments. Conclusion: The combination of thermotherapy technique associated with the culture of meristems constitutes an effective means useful for the sanitation of infected plant material.

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