scholarly journals Fertilisation rate obtained with frozen-thawed boar semen supplemented with rosmarinic acid using a single insemination timed according to vulvar skin temperature changes

2015 ◽  
Vol 63 (1) ◽  
pp. 100-109 ◽  
Author(s):  
Victoria Luño ◽  
Lydia Gil ◽  
Maite Olaciregui ◽  
Juan Grandía ◽  
Trinidad Ansó ◽  
...  

Artificial insemination (AI) of sows with frozen-thawed semen usually results in lower pregnancy rates and litter sizes than the use of liquid preserved semen. The present study evaluated the effectiveness of vulvar skin temperature changes as a predictor of ovulation in sows and determined the fertility rates obtained after AI with frozen-thawed semen supplemented with rosmarinic acid (RA). Semen was collected from mature boars and cryopreserved in experimental extenders supplemented with or without 105 μM of RA. Multiparous sows were inseminated with a single dose of semen when vulvar skin temperature decreased to a value below 35 °C. Intrauterine insemination was performed using 1.5 × 109 spermatozoa. The sows were slaughtered 48 h after AI and the embryos and oocytes were recovered from the oviducts. Total and progressive motility, viability and acrosome integrity were significantly (P < 0.05) higher in RA-supplemented semen samples compared with the control. Fertilisation occurred in all sows inseminated in the study, although there were no significant differences between the experimental groups. Sows inseminated with RA-supplemented semen showed a slight increase in the number of embryos recovered as compared to sows inseminated with control semen. In conclusion, insemination according to vulvar skin temperature changes resulted in successful fertilisation in all sows, although supplementation of the freezing media with RA did not improve the fertilising ability of frozen-thawed boar sperm.

2016 ◽  
Vol 136 (11) ◽  
pp. 1581-1585 ◽  
Author(s):  
Tota Mizuno ◽  
Takeru Sakai ◽  
Shunsuke Kawazura ◽  
Hirotoshi Asano ◽  
Kota Akehi ◽  
...  

2012 ◽  
Vol 30 (1) ◽  
pp. 27-31 ◽  
Author(s):  
Hidetoshi Mori ◽  
Tim Hideaki Tanaka ◽  
Hiroshi Kuge ◽  
Ken Sasaki

Objective To determine whether any difference exists in responses to indirect moxibustion relative to thermal stimulation sites. Methods Twenty one healthy men of mean±SD age 22.5±6.1 years were randomly divided into two groups, one receiving a single moxibustion stimulation in three locations (the three-point stimulation group, n=10 participants) and the other receiving three stimulations in one location (the one-point stimulation group, n=11 participants). The thermal stimulation sites were GV14, GV9 and GV4 acupuncture points. A thermograph was used to obtain the skin temperature on the posterior trunks of the participants. To analyse skin temperature, four arbitrary frames (the scapular, interscapular, lumbar and vertebral regions) were made on the posterior trunk. Result An increase in skin temperature on the posterior trunk was observed following both one- and three-point moxibustion administrations. The skin temperature of the lumbar region showed a significant increase after three-point stimulation compared with single-point stimulation (p=0.011). There was also a significant increase in skin temperature of the spinal region after three-point stimulation compared with one-point stimulation (p=0.046). Conclusion Administration of single moxibustion doses on the GV14, GV9 and GV4 points produces greater changes in skin temperature than three applications of moxibustion to the GV14 point only.


2021 ◽  
Vol 55 (4) ◽  
pp. 246
Author(s):  
Gede Wira Buanayuda ◽  
Hamdani Lunardhi ◽  
Indra Gusti Mansur

Infertility is a problem for husband and wife, in the last 20 years the number of infertile couples has tended to increase by around 6.5 million pairs. The infertile couple can use the intrauterine insemination method to obtain offspring if a conventional method approach cannot be performed. Insemination requires a sperm preparation stage in which there are centrifugation and resuspension procedures that tend to produce excess reactive oxygen species (ROS). Excessive ROS will damage the motility of the spermatozoa. This study aims to prove the addition of alpha lipoic acid (ALA) as an antioxidant in the process of sperm preparation to improve and maintain better sperm motility. This research is a laboratory study with an experimental research design. The sample consisted of 10 infertile men who visited the Andrology section of the Sayyidah Jakarta Mother and Child Hospital (RSIA), where each ejaculate from the patient would be divided into 3 groups namely (k1) fresh semen as a control group, (k2) sperm preparation group without ALA, (k3) group of sperm preparation with the addition of ALA. The motility of spermatozoa was observed with the WHO 1999 method for 4 hours in units of percent. Progressive motility in k3 (47.95 ± 3.617) was higher than in k2 (38.05 ± 3.278) statistically significantly different after 3 hours of observation (p<0.0001). Progressive motility in k3 (78.8 ± 5.841) was higher than k1 (56.55 ± 7.511) from the initial observation (p <0.0001). The progressive motility of k2 (76.05 ± 6.768) was higher than k1 (56.55 ± 7.511) from the start of the observation (0.0001). It can be concluded that the addition of ALA in the sperm preparation process increases and maintains progressive motility that is better than sperm preparation without ALA addition after 3 hours of observation.


2005 ◽  
Vol 17 (2) ◽  
pp. 159 ◽  
Author(s):  
E.A. Martinez ◽  
J.M. Vazquez ◽  
I. Parrilla ◽  
C. Cuello ◽  
M.A. Gil ◽  
...  

A new procedure for nonsurgical deep intrauterine insemination (DUI) in non-sedated sows has recently been reported (Martinez et al. 2002 Reproduction 123, 163–170). In comparison to traditional artificial insemination (AI), using this procedure, a 20-fold reduction in the number of spermatozoa inseminated can be used without a decrease in fertility when hormonally treated post-weaning estrous sows are used. The aim of the present study was to evaluate the effectiveness of DUI under field conditions. In Experiment 1, crossbred sows (2–6 parity) were weaned at 20.75 ± 0.06 days. Estrous detection was performed once per day, beginning 3 days after weaning. Sows with a weaning to estrus interval of 4–5 days were selected to be inseminated. A total of 190 sows were inseminated at 12, 24, and 36 h after onset of estrus using one of the following two regimes: (1) DUI with 150 × 106 fresh spermatozoa in 5 mL of BTS (n = 95) and (2) Traditional AI with 3 × 109 fresh spermatozoa in 100 mL of BTS (n = 95) prepared from the same semen samples used for the DUI group. Farrowing rates (FR) and litter sizes (LTS; mean ± SEM) from both groups were compared using chi-squared test and ANOVA, respectively. There was no significant difference in the FR between groups (83.2 and 86.3% for DUI and AI groups, respectively). However, a decrease (P < 0.001) in the LTS was observed in sows inseminated by the DUI procedure (9.8 ± 0.29 and 10.9 ± 0.17, respectively). In Experiment 2, seventy one natural post-weaning estrus sows were used. Fifty-five sows were DUI inseminated three times with 150 (n = 17), 300 (n = 19), or 600 (n = 19) × 106 spermatozoa in 5, 10, or 20 mL of BTS, respectively. The remaining sows (n = 16) were traditionally inseminated. On Day 6 after estrus, sows were subjected to laparotomy and the tips of both uterine horns were flushed in order to evaluate pregnancy rate (PR: percentage of sows with at least 4 viable embryos) and fertilization rate (ratio of viable embryos to the total number of embryos and oocytes). PR was similar in all the groups, ranging from 84.2% (DUI 300 × 106 spermatozoa group) to 94.7% (DUI 600 × 106 spermatozoa group). Fertilization rate and the percentage of bilateral fertilization after DUI with 600 × 106 spermatozoa did not differ from those of the AI group (97.8 and 100% vs. 98.4 and 100%, respectively), but a significant decrease in both parameters (P < 0.05; chi-square test) was observed in sows inseminated with 300 (94.3 and 87.5%) or 150 (84.4 and 66.7%) × 106 spermatozoa. In conclusion, DUI with 150 × 106 spermatozoa offers similar FR but a lower LTS in sows with natural estrus in comparison with those parameters obtained when traditional AI is used. The lower litter size could be related to the low percentage of bilateral fertilization observed in that group. This work was supported by CDTI 020003.


Author(s):  
K. G. Ambily ◽  
Malati Naik ◽  
Hiron M. Harshan ◽  
C. Jayakumar ◽  
M. P. Unnikrishnan ◽  
...  

Boar semen is voluminous and ejaculated as jets or fractions of pre-sperm, sperm rich (SRF) and post-sperm rich fractions. Recent studies have reported more resilient characteristics of sperm in initial portions of SRF towards cold shock and cryopreservation. The present study was conducted to assess the quality of specific fractions of SRF, namely, first 10mL of SRF (F1) and rest of SRF (F2) in Large white Yorkshire (LWY) boar semen. Ejaculates were collected using gloved-hand technique and were subjected to quality assessments of volume, pH, sperm progressive motility, concentration, plasma membrane integrity, abnormality, acrosome integrity and sperm membrane cholesterol. Upon statistical analysis, significant differences were noticed in volume, pH, sperm concentration and sperm membrane cholesterol between fractions of the ejaculate.


2017 ◽  
Vol 53 ◽  
pp. 254-259
Author(s):  
V. O. Melnik ◽  
O. O. Kravchenko ◽  
О. S. Kohut

Improving of fertility indicators and reproductive qualities of sows during artificial insemination in farms of different specializations is very topical issue. The introduction of artificial insemination of sows on breeding farms by the spermdoses of optimum volume, by the frozen-thawed and sex sperm requires the introduction of innovative reproductive technologies. The use of economical methods of artificial insemination of sows using a minimum number of sperm in a small volume of spermdose in order to achieve high rates of fertility and prolificacy was proven in numerous experiments of the authors. Significantly reduced spermdose may be sufficient if the sperm enters deep enough into the uterus. Vitality of sperm does not depend on the size of spermdose, but the best place for sperm to survive one oviducts where they keep the fertilizing capacity from 9 to 27 hours. So deep intrauterine insemination of sows improves conditions for sperm survival The aim was to study the feasibility and justification for widespread implementation in to production on breeding farms of intrauterine insemination of sows morder to increase their fertilization and prolificacy and  to save the boar sperm with the highest index of breeding values. Experiments were conducted in terms of selection and genetic center of Agrofirm "Mig-Service-Agro" in Mykolaiv region. In the experiment used 65 sows of live weight of 280-320 kg wiht 2-4 farrowing were. Sows in sexual hunting were showed once daily in the morning using a boar-prober. Artificial insemination was performed twice: the first time - in the afternoon and at 14-16 p.m. The second time – in the morning of the next day at 9-10 am. For artificial insemination of sows were ed using experimental spermodes with volume of 40 ml which contained 1.5 billion of a Active sperm. For the dilution of sperm was used Durasperm - KRUUSE (Denmark) the period of sperm perpetuation is 5-7 days. To enter the were semen used catheters Magaplus S, (Spain) for intrauterine insemination of sows. Analysis shows that the period from weaning to insemination has significant difference comparing sows of large White breed with Landrace breed (p <0.001), with genotype sows F1 (p <0.01) and sows of the Duroc breed (p <0.05). For all selected 65 sows duration of suckling period, was estimated which averaged 32.2 days and the average time from weaning of pigs to their sexual inclination and the first intrauterine insemination 6.8 days that meets the physiological norm. After intrauterine insemination of sows of then 48 farrowed, which wich made for 73.9%. іncluding live 5 emergency farrow  were obtained, representing 10.4% of all amount. Percentage of farrow is considered physiologically normal – 80%, or more of total insemined sows. The very low percentage of farrow 53.3% had of sows F1, and the highest percentage was found by sows of the Duroc breed – 85.7%. Pregnency of sows were received just 17, which made 26.1% and highest percentage – 46.7% was set by sows F1. Analysis of the pregnancy sows shows that on average it is 116.2 days was the longest – 117.1 days was set in Landrace breed sows and the short est 115.5 days in Large White breed, but the difference is not significant. 571 pigs were received, іncluding live 451 head, which is 78.9%. The largest percentage of іncluding live piglets obtained from sows F1 – 82.5%, and the lowest in Landrace breeds – 77.0% and Large White – 77.3%. Exit of all piglets per sow without emergency farrowings is 12.2, іncluding live – 9,8. The highest yield were obtained piglets from sows F1 – 13.1, іncluding live – 10.9, the lowest yield of sows of the Duroc breed – 10.6, іncluding live 9.1, which has significant difference compared with the control (IDPs) and other breeds.  After intrauterine insemination 8 sows showed cyclic deregulation in 20-25 days, ie repeated sexual hunt took place on average 22.3 days. These sows were inseminated by not fractional way, they farrowed and an average litter just 13.1 piglets per sow, іncluding live - 11.3 was obtained. Repeating after intrauterine insemination on 45-48-49 day in the sexual hunt came about three sows for artificial insemination by not fractional method 3 farrowed and was obtained output – 13.3 piglets, іncluding live – 11.7. It should be noted that the best sow Large White breed №12 after intrauterine insemination bore 16 pigs, іncluding live 11, sow of Landrace breed №1556 – 18 pigs, іncluding live 12, Duroc №5888 – 13 pigs, іncluding live 11, sow F1 №167 – 20 pigs, іncluding live 14 pigs. Breeding requires more careful handling with major sows taking into account their breeding value and cost, that’s why we believe that there is no need to risk causing injury genitals with intrauterine insemination if a sufficient number of spermdoses of boars-sires exist.


Maturitas ◽  
1986 ◽  
Vol 8 (1) ◽  
pp. 67-76 ◽  
Author(s):  
Michael J. Katovich ◽  
James W. Simpkins ◽  
Lee Ann Berglund ◽  
June O'Meara

2017 ◽  
Vol 9 (4) ◽  
pp. 163-168 ◽  
Author(s):  
Jonathan M. Burke ◽  
Austin R. Herman ◽  
Blaine C. Long ◽  
Kevin C. Miller

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