Bagian Ilmu Kesehatan Gigi dan Mulut Fakultas Kedokteran Gigi Universitas Hasanuddin Makassar, Indonesia

Elderly people are particularly vulnerable to oral disease. Decreased function of the body can affect the aging process of the organic, and anorganic content in the elderly. The aim of this study was to determine the content of organic elements (total protein level), volume and pH of saliva in the elderly. This descriptive observational study with crosssectional design was performed in Tresna Werdha Gau Mabaji Social Institution, Gowa (n=30)with a simple random sampling technique using 30% of the total population and in Werdha Theodora Makassar nursing home with saturated sampling technique (n = 13). Anorganic contents were measured using spectophotometric and organic content of the total protein level of saliva was measured using an autoanalyzer tool kyltec in BPTP Laboratory Maros. Statistical analysis was performed with t-test using SPSS version 16. There were no significant differences between the anorganic (Ca2+,Mg2+, and phosphate) and organic elements content of saliva (total protein level), the volume of saliva and salivary pH in the elderly by sex and age groups. Yet, there are significant differences in the volume of saliva of elderly who consume drugs for systemic diseases. It is concluded that gender and age do not affect the anorganic (Ca,and phosphate)and organic element content of saliva (total protein content), the volume of saliva, and saliva pH in the elderly. Drugs consumed for systemic diseases does not affect the anorganic and organic element content of saliva and the pH of saliva in the elderly.

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Dynamics of individual indicators of protein metabolism in the body of broiler chickens on the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”

Ukrainian Journal of Veterinary and Agricultural Sciences ◽

10.32718/ujvas3-2.07 ◽

2020 ◽

Vol 3 (2) ◽

pp. 42-46

Author(s):

V. G. Stoyanovskyy ◽

M. O. Shevchuk ◽

I. A. Kolomiiets ◽

V. A. Kolotnytskyy

Keyword(s):

Total Protein ◽

Protein Metabolism ◽

Broiler Chickens ◽

Stress Factor ◽

The Body ◽

Control Group ◽

Total Protein Content ◽

Combined Stress ◽

Stages Of Development ◽

Γ Globulins

The body of broiler chickens is characterized by a high metabolism, which promotes rapid growth and development, but their performance indicators are largely related to housing conditions, which are known to include a number of technological stressors. With the development of stress in the body of poultry changes the course of metabolic processes, which causes changes in all types of metabolism, including protein. The aim of the study was to determine the changes in individual indicators of protein metabolism in the body of broiler chickens against the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”. At 13th day of life, all clinically healthy poultry were exposed to combined stress – revaccination (intranasal Newcastle disease) plus cold stress (for 60 minutes by air conditioning and 5 °C in the vivarium). Material for research was selected for 3 days after the action of the stress factor (stage of anxiety), 13, 20 and 26 days after the action of the stress factor (the resistance stage). The material for the study was blood plasma, which determined the concentration of total protein, as well as the content of albumin, globulin fraction (α1, α2, β, γ). It was found that 3 days after the action of stress in chickens of Control group the development of adaptive reactions is manifested by the stability of the total protein content and redistribution of the fractional composition of plasma globulins in the form of increased albumin and α2-globulins. At different stages of development of the stage of resistance in birds of Control group, the content of total protein decreases by an average of 14.6 % due to the content of albumin by 6.9 % and α2-globulins – by 15.9 %, against the background of increasing α1- and γ-globulins by 23.1 and 33.5 % with the stabilization of individual studied indicators at the final stages of development of the resistance stage. The use in the diet of broilers feed “Reasil Humic Vet”, probiotic feed supplement “Laktin”, feed “Reasil Humic Health” under the influence of complex stress helps to increase the intensity of protein metabolism in poultry with the development of adaptation syndrome, as evidenced by the growth of total protein on average by 37.8 % (P < 0.05) and albumin – by 17.0 % (P < 0.05), which indicates an increase in the intensity of protein-synthesizing properties of the organism. In different periods of stress in the blood of broiler chickens Research groups there is an increase in the ratio of individual protein fractions, especially the content of γ-globulins - an average of 21.3 % (P < 0.05), which indicates an increase in the immune status of their body with a predominance of numerical values in the Research 1 group of poultry.

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An atherogenic stimulus homocysteine inhibits cofactor activity of thrombomodulin and enhances thrombomodulin expression in human umbilical vein endothelial cells

Blood ◽

10.1182/blood.v79.11.2930.2930 ◽

1992 ◽

Vol 79 (11) ◽

pp. 2930-2936 ◽

Cited By ~ 110

Author(s):

T Hayashi ◽

G Honda ◽

K Suzuki

Keyword(s):

Endothelial Cells ◽

Total Protein ◽

Protein Level ◽

Umbilical Vein ◽

Mrna Level ◽

Fold Increase ◽

Immunoblot Analysis ◽

Whole Cells ◽

Total Protein Level ◽

Cofactor Activity

Abstract Thrombomodulin plays a role as a cofactor for thrombin-catalyzed activation of protein C on endothelial cells. We examined the effect of homocysteine, a stimulant of atherosclerosis and thrombotic disease, on the cofactor activity and protein level of thrombomodulin and also on the expression of thrombomodulin in endothelial cells. Homocysteine inhibited the cofactor activity of thrombomodulin both on the surface of endothelial cells and in the whole cells dose- and time-dependently, and maximal inhibition of the cofactor activity occurred after a 3- to 6-hour incubation with 10 mmol/L homocysteine (10% of initial activity). Homocysteine also decreased the amount of intact (unreduced) thrombomodulin in endothelial cells. However, at the same condition the total protein level (reduced and unreduced form) of thrombomodulin, determined by dot immunoblot analysis using the monoclonal antibody that recognized both reduced and unreduced thrombomodulin, decreased slightly, and the mRNA level of thrombomodulin showed a twofold to three-fold increase. After 24 hours of incubation, the cofactor activity and total protein level of thrombomodulin were 60% and 165% of the initial values, respectively. When purified thrombomodulin fixed to a microwell plate was treated with homocysteine, both cofactor activity and thrombin-binding ability to the thrombomodulin were decreased in proportion to the concentration of homocysteine. These findings suggest that homocysteine directly inhibited the cofactor activity of thrombomodulin on endothelial cells by reducing the disulfide-bond rich epidermal growth factor-like structures of thrombomodulin. This would a result in the decrease of the antithrombotic property of endothelium and may also trigger off the synthesis of mRNA and protein of thrombomodulin to maintain the antithrombotic properties of the cells.

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Changes in the composition of proteins in the blood during the period of ischemia-reperfusion syndrome, modeled by a violation of blood flow in the liver

Vestnik of Russian military medical Academy ◽

10.17816/brmma12275 ◽

2018 ◽

Vol 20 (2) ◽

pp. 110-114

Author(s):

G Sh Garayev ◽

J R Gafarova ◽

R E Jafarova

Keyword(s):

Total Protein ◽

Plasma Proteins ◽

Ischemia Reperfusion ◽

Protein Composition ◽

The Body ◽

Total Protein Content ◽

Quantitative Composition ◽

Liver Ischemia ◽

Pathological Changes ◽

Quantitative Changes

On the experimental model of liver ischemia-reperfusion, quantitative changes in protein composition in the blood of white outbred rats were investigated. It was found that against the background of a 10-minute ischemia, the total protein content decreased by 0,9%, albumins by 1,5% (p0,05). The content of globulins practically did not change, and the content of fibrinogen increased by 33,3% (p0,05). In the corresponding periods of reperfusion compared with intact values, the content of total protein and albumins decreased, and globulins and fibrinogen increased. The intensity of changes in the quantitative and qualitative content of proteins in the blood of animals depended on both the duration of the ischemia period and the duration of reperfusion. At the same time, the increase in the level of globulins in the blood occurred more intensively during reperfusion after 20 minutes of ischemia. Thus, against the background of ischemia, there are changes in the quantitative composition of plasma proteins (total protein, albumins, globulins, fibrinogen). As the duration of ischemia increases, pathological changes increase. With reperfusion, the process continues unidirectionally, but against the background of a deeper ischemia lasting 20 minutes, some parameters of the pathological changes in the blood composition are less pronounced than against a background of reperfusion after a 10-minute ischemia. This phenomenon is explained by the activation of the protective and adaptive functions of the body.

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An atherogenic stimulus homocysteine inhibits cofactor activity of thrombomodulin and enhances thrombomodulin expression in human umbilical vein endothelial cells

Blood ◽

10.1182/blood.v79.11.2930.bloodjournal79112930 ◽

1992 ◽

Vol 79 (11) ◽

pp. 2930-2936 ◽

Cited By ~ 1

Author(s):

T Hayashi ◽

G Honda ◽

K Suzuki

Keyword(s):

Endothelial Cells ◽

Total Protein ◽

Protein Level ◽

Umbilical Vein ◽

Mrna Level ◽

Fold Increase ◽

Immunoblot Analysis ◽

Whole Cells ◽

Total Protein Level ◽

Cofactor Activity

Thrombomodulin plays a role as a cofactor for thrombin-catalyzed activation of protein C on endothelial cells. We examined the effect of homocysteine, a stimulant of atherosclerosis and thrombotic disease, on the cofactor activity and protein level of thrombomodulin and also on the expression of thrombomodulin in endothelial cells. Homocysteine inhibited the cofactor activity of thrombomodulin both on the surface of endothelial cells and in the whole cells dose- and time-dependently, and maximal inhibition of the cofactor activity occurred after a 3- to 6-hour incubation with 10 mmol/L homocysteine (10% of initial activity). Homocysteine also decreased the amount of intact (unreduced) thrombomodulin in endothelial cells. However, at the same condition the total protein level (reduced and unreduced form) of thrombomodulin, determined by dot immunoblot analysis using the monoclonal antibody that recognized both reduced and unreduced thrombomodulin, decreased slightly, and the mRNA level of thrombomodulin showed a twofold to three-fold increase. After 24 hours of incubation, the cofactor activity and total protein level of thrombomodulin were 60% and 165% of the initial values, respectively. When purified thrombomodulin fixed to a microwell plate was treated with homocysteine, both cofactor activity and thrombin-binding ability to the thrombomodulin were decreased in proportion to the concentration of homocysteine. These findings suggest that homocysteine directly inhibited the cofactor activity of thrombomodulin on endothelial cells by reducing the disulfide-bond rich epidermal growth factor-like structures of thrombomodulin. This would a result in the decrease of the antithrombotic property of endothelium and may also trigger off the synthesis of mRNA and protein of thrombomodulin to maintain the antithrombotic properties of the cells.

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Socioeconomic, Nutritional Profile Correlates with Morbidity Risks and Quality of Health Status among Elderly Residing at Old Age Home & Residence from Different Region of South Karnataka

International Journal of Health Sciences and Research ◽

10.52403/ijhsr.20210652 ◽

2021 ◽

Vol 11 (6) ◽

pp. 349-355

Author(s):

Pooja Anudhar G ◽

Sushma B. V

Keyword(s):

Nutritional Status ◽

Old Age ◽

Sampling Technique ◽

The Elderly ◽

The Body ◽

Deficiency Anemia ◽

Elderly Subjects ◽

Randomized Sampling ◽

Nutritional Profile

Ageing is the multidimensional process in humans with changes occurring in physical, psychological and social aspects which increase their proneness to health ailments. The associated functional impairment and physical inability will influence on their absorption and metabolism leading to the changes in nutritional status. Epidemiological study was conducted among the elderly subjects with respective to understand the quality of health in relation to the nutritional profile. Using a purposive randomized sampling technique, the elderly subjects were selected and assessed for the nutrition and health condition. The investigation comprises approximately 90 subjects of old age institutions and 90 subjects of residents from different domiciles. The study was conducted using standardized validated tools and checklist with oral interview technique and one to one interactions. Findings: In accordance with the body mass evaluation; approximately 59% elderly living at own houses were under Grade 1, Grade 2 and Grade 3 obesity in comparison to their counterpart. Medical health records indicated iron deficiency anemia among 10% of subjects. Gastritis, Diabetes, hypertension, arthritis was observed to be the major health problems. Comparatively 64% male elderly from household had oral problems and underwent for dentures. Often had three meal patterns in a day. Henceforth, there is a need to understand and address on the nutritional needs, etiology and demographic characteristics interlinked with comorbidities and health disabilities among aging population. Evidently a significant association was noticed between nutritional status on morbidities and health quality among elderly. Key words: Old age home, elderly, Ageing, nutritional profile, quality of health.

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ELEKTROFORESIS PROTEIN SERUM PASIEN DENGAN KADAR PROTEIN NORMAL

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY ◽

10.24293/ijcpml.v15i3.962 ◽

2018 ◽

Vol 15 (3) ◽

pp. 87

Author(s):

Tiene Rostini ◽

Coriejati Rita

Keyword(s):

Serum Protein ◽

Total Protein ◽

Protein Level ◽

Protein Electrophoresis ◽

Serum Protein Electrophoresis ◽

Total Protein Level ◽

Normal Limits ◽

Nephritic Syndrome ◽

Serum Protein Level ◽

Electrophoresis Pattern

Serum protein electrophoresis pattern can assist in diagnosis of liver disease, hematological disorders, renal disorders andgastrointestinal disease. Measurement of total protein level in the serum cannot detect any disorders in patient with normal limit ofserum total protein level. The aim of this study; was to evaluate the serum protein electrophoresis pattern in patient with normal limitsof serum protein level. This research was carried out by descriptive retrospective study using the electrophoresis data from patients’medical record at the Clinical Pathology Department, Dr. Hasan Sadikin General Hospital Bandung. The data of serum electrophoresis (bySebia gel electrophoresis) were grouped based on disease or disorders, and confirmed with the diagnosis derived from patient’s medicalrecord. Inclusion criteria of samples if ; the electrophoresis data were available, serum total protein level within normal limits (6.4–8.3mg/dL), and the data of electrophoresis taken from medical record were taken from August 2006 until August 2008. The result foundso far was, there were 240 data of electrophoresis from patients with serum protein level within normal limits (6.4–8.3 mg/dL). theinterpretation of electrophoresis consist of: 1) inflammation (149 patients; 62.2% ; sensitivity 83.7%, specificity 86,5%) 2) Cirrhosis(46 patients ; 19.2% ; sensitivity 87.5% ; specificity 88.4%) 3) Nephritic syndrome (15 patients ; 6.2%; sensitivity 53%; specificity96.9% 4) Monoclonal gammophaty (15 patients(6.2% ; sensitivity 80% ; specificity 98.7%) 5) Normal pattern in 15 patient (6.2%).This study found abnormal serum protein electrophoresis pattern in the condition of inflammation, Cirrhosis, Nephritic Syndrome, andMonoclonal gammophaty. It can be concluded that many disorders could be detected in patient with serum protein level within normallimits such as: inflammation, cirrhosis, nephritis syndrome and monoclonal gammophaty by abnormal electrophoresis pattern

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Marker changes of blood plasma proteinogram in rats with toxic hepatitis

Regulatory Mechanisms in Biosystems ◽

10.15421/022055 ◽

2020 ◽

Vol 11 (3) ◽

pp. 360-366

Author(s):

V. A. Gryshchenko ◽

V. S. Minina

Keyword(s):

Blood Plasma ◽

Plasma Protein ◽

Total Protein ◽

Toxic Hepatitis ◽

Diclofenac Sodium ◽

The Body ◽

Laboratory Animals ◽

Total Protein Content ◽

Protein Spectrum ◽

G Ratio

In recent years, there has been a pronounced tendency to increase in the incidence of drug-induced liver damage due to the growing expansion of the pharmaceutical market, which is also observed in the case of incorrect administration of nonsteroidal anti-inflammatory drugs (NSAIDs). In this case, the violation of the functional state of the body has a negative effect on synthetic processes, which in combination with the protein system of tissues significantly affects the metabolic homeostasis of the body. Therefore, the aim of the study was to determine marker changes in the plasma protein spectrum in laboratory rats with diclofenac-induced hepatitis and the effectiveness of reparative therapy based on milk phospholipids. The drug form of toxic hepatitis in laboratory animals was induced according to the author’s model by oral administration of diclofenac sodium (NSAID group) at a dose of 12.5 mg/kg, once a day for 14 days. Thus, in rats with toxic hepatitis there was a probable decrease in plasma total protein content by 15.6% compared with control, indicating a violation of protein-synthesizing function of the liver. With the introduction into the body of clinically healthy and sick animals of the liposomal form of the bioadditive "FLP-MD" based on milk phospholipids, the level of total protein in blood plasma corresponded to control values. As a result of the study of the plasma protein spectrum of Wistar rats, the four most sensitive indicators, which undergo significant probable changes in absolute and relative units of measurement with the development of toxic diclofenac-induced hepatitis, are protein fractions with molecular weights of 180–190, 150–170, 60 and 54–58 kDa and four markers of the effectiveness of restoring the protein-synthesizing function of the liver with the use of corrective therapy, in particular, bioadditives "FLP-MD" – 900, 180–190, 68–70 kDa and the value of A/G ratio, which is important for implementation in applied veterinary medicine, especially in the diagnosis of NSAID hepatopathy, supplementing the picture of its pathogenesis at the molecular level and testing the effectiveness of newly created drugs of hepatoprotective profile.

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Protective Effect of Terminalia Chebula Extract on Serum Total Protein against

Bangladesh Medical Journal ◽

10.3329/bmj.v46i1.34632 ◽

2017 ◽

Vol 46 (1) ◽

pp. 11-14

Author(s):

Tania Yeasmin ◽

Kazi Shamima Akhter ◽

Masud Imtiaz

Keyword(s):

Protective Effect ◽

Total Protein ◽

Protein Level ◽

Control Group ◽

Albino Rats ◽

Terminalia Chebula ◽

Serum Total Protein ◽

Medical College ◽

Total Protein Level ◽

Serum Total Protein Level

Terminalia Chebula extract is used for regeneration of hepatic cells and protection of liver against damage due to its active component. This study aims to observe the protective effect of Terminalia Chebula against Paracetamol induced change of serum total protein level in Wister Albino rats. The study was carried out in the Department of Physiology, Dhaka Medical College (DMC) during January 2013 to December 2013. A total number of 44 rats, age ranging from 90 to 120 days, weight between 150 to 200 gm (initial body weight) were selected for the study. After acclimatization for 14 days, they were divided into control groups and experimental groups. Before sacrifice, final body weights of all the rats were measured. then all the rats were sacrificed on 22nd day and then blood samples were collected. For assessment of liver function, serum total protein level was done by using standard laboratory kits. The mean serum total protein level was significantly (p<0.001) lower in paracetamol treated control group in comparison to those of baseline control group. Serum total protein level of all experimental groups were significantly (P<0.001) higher than Paracetamol treated control group. From the results of this study, it may be concluded that Terminalia Chebula may have some protective effect against Paracetamol induced liver damage in rats.Bangladesh Med J. 2017 Jan; 46 (1): 11-14

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