scholarly journals Differentiation of Bone Marrow Stem Cells on Inkjet Printed Silk Lines

2006 ◽  
Vol 950 ◽  
Author(s):  
Skander Limem ◽  
Paul Calvert ◽  
Hyeon Joo Kim ◽  
David L. Kaplan
Keyword(s):  
Bone Marrow ◽  
Inkjet Printing ◽  
Culture Cell ◽  
Bioactive Materials ◽  
Plastic Substrates ◽  
Cell Functions ◽  
Printed Patterns ◽  
First Time ◽  
Vinyl Plastic ◽  
Printing Techniques

ABSTRACTWater based silk solutions were successfully inkjet printed for the first time into patterns of parallel lines onto vinyl plastic substrates. Human bone marrow stromal cells (hMSCs) were seeded on the silk printed patterns and cultured in the presence of 100 ng/ml of bone morphogenic protein (BMP-2). After one week of culture cell growth and attachment showed site specificity on the silk printed lines. Both alkaline phosphatase activity and cell morphology indicated hBMSCs differentiation into osteogenic cells along the silk printed lines. After 4 week of culture, the cellular bridging of adjacent silk printed lines took place for all interline distances lower than 1.25 mm. Therefore, commercial inkjet printing technology can produce complex viable cellular patterns with 111 ± 24 μm lateral resolution, through the deposition of bioactive materials. The results provide a first step toward cell specific control using 3D inkjet printing techniques using biocompatible gel systems to regulate cell functions.

scholarly journals Aberrant Expression of TLR2, TLR7, TLR9, Splicing Variants of TLR4 and MYD88 in Chronic Lymphocytic Leukemia Patients

2021 ◽  
Vol 10 (4) ◽  
pp. 867
Author(s):  
Katarzyna Skorka ◽  
Paulina Wlasiuk ◽  
Agnieszka Karczmarczyk ◽  
Krzysztof Giannopoulos
Keyword(s):  
Bone Marrow ◽  
Chronic Lymphocytic Leukemia ◽  
Cytotoxic T Cells ◽  
Lymphocytic Leukemia ◽  
Aberrant Expression ◽  
Downstream Signaling ◽  
Splicing Variants ◽  
High Level ◽  
Time To First Treatment ◽  
First Time

Functional toll-like receptors (TLRs) could modulate anti-tumor effects by activating inflammatory cytokines and the cytotoxic T-cells response. However, excessive TLR expression could promote tumor progression, since TLR-induced inflammation might stimulate cancer cells expansion into the microenvironment. Myd88 is involved in activation NF-κB through TLRs downstream signaling, hence in the current study we provided, for the first time, a complex characterization of expression of TLR2, TLR4, TLR7, TLR9, and MYD88 as well as their splicing forms in two distinct compartments of the microenvironment of chronic lymphocytic leukemia (CLL): peripheral blood and bone marrow. We found correlations between MYD88 and TLRs expressions in both compartments, indicating their relevant cooperation in CLL. The MYD88 expression was higher in CLL patients compared to healthy volunteers (HVs) (0.1780 vs. 0.128, p < 0.0001). The TLRs expression was aberrant in CLL compared to HVs. Analysis of survival curves revealed a shorter time to first treatment in the group of patients with low level of TLR4(3) expression compared to high level of TLR4(3) expression in bone marrow (13 months vs. 48 months, p = 0.0207). We suggest that TLRs expression is differentially regulated in CLL but is similarly shared between two distinct compartments of the microenvironment.

scholarly journals Additive Manufacturing for Effective Smart Structures: The Idea of 6D Printing

2021 ◽  
Vol 5 (5) ◽  
pp. 119
Author(s):  
Stelios K. Georgantzinos ◽  
Georgios I. Giannopoulos ◽  
Panteleimon A. Bakalis
Keyword(s):  
Additive Manufacturing ◽  
Degrees Of Freedom ◽  
Smart Structures ◽  
Interaction Mechanism ◽  
Modeling And Simulations ◽  
Environmental Stimulus ◽  
Material Component ◽  
Design And Manufacturing ◽  
First Time ◽  
Printing Techniques

This paper aims to establish six-dimensional (6D) printing as a new branch of additive manufacturing investigating its benefits, advantages as well as possible limitations concerning the design and manufacturing of effective smart structures. The concept of 6D printing, to the authors’ best knowledge, is introduced for the first time. The new method combines the four-dimensional (4D) and five-dimensional (5D) printing techniques. This means that the printing process is going to use five degrees of freedom for creating the final object while the final produced material component will be a smart/intelligent one (i.e., will be capable of changing its shape or properties due to its interaction with an environmental stimulus). A 6D printed structure can be stronger and more effective than a corresponding 4D printed structure, can be manufactured using less material, can perform movements by being exposed to an external stimulus through an interaction mechanism, and it may learn how to reconfigure itself suitably, based on predictions via mathematical modeling and simulations.

scholarly journals Strain difference of murine bone marrow-derived mast cell functions

2005 ◽  
Vol 78 (3) ◽  
pp. 605-611 ◽  
Author(s):  
Junko Noguchi ◽  
Etsushi Kuroda ◽  
Uki Yamashita
Keyword(s):  
Bone Marrow ◽  
Mast Cell ◽  
Strain Difference ◽  
Murine Bone Marrow ◽  
Cell Functions

Printing ultrathin graphene oxide nanofiltration membranes for water purification

2017 ◽  
Vol 5 (39) ◽  
pp. 20860-20866 ◽  
Author(s):  
Mahdi Fathizadeh ◽  
Huynh Ngoc Tien ◽  
Konstantin Khivantsev ◽  
Jung-Tsai Chen ◽  
Miao Yu
Keyword(s):  
Graphene Oxide ◽  
Water Purification ◽  
Inkjet Printing ◽  
Low Cost ◽  
Nanofiltration Membranes ◽  
Highly Effective ◽  
First Time ◽  
Polymeric Supports ◽  
Effective Water

We demonstrated for the first time that inkjet printing can be a low-cost, easy, fast, and scalable method for depositing ultrathin (7.5–60 nm) uniform graphene oxide (GO) nanofiltration membranes on polymeric supports for highly effective water purification.

scholarly journals Myelin Debris Stimulates NG2/CSPG4 Expression in Bone Marrow-Derived Macrophages in the Injured Spinal Cord

2021 ◽  
Vol 15 ◽  
Author(s):  
Yang Liu ◽  
Grace Hammel ◽  
Minjun Shi ◽  
Zhijian Cheng ◽  
Sandra Zivkovic ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Bone Marrow ◽  
Spinal Cord Injury ◽  
Phagocytic Capacity ◽  
Myelin Sheaths ◽  
Cellular Debris ◽  
Cord Injury ◽  
And Function ◽  
Secondary Spinal Cord Injury ◽  
First Time

Although the increased expression of members of the chondroitin sulfate proteoglycan family, such as neuron-glial antigen 2 (NG2), have been well documented after an injury to the spinal cord, a complete picture as to the cellular origins and function of this NG2 expression has yet to be made. Using a spinal cord injury (SCI) mouse model, we describe that some infiltrated bone marrow-derived macrophages (BMDMΦ) are early contributors to NG2/CSPG4 expression and secretion after SCI. We demonstrate for the first time that a lesion-related form of cellular debris generated from damaged myelin sheaths can increase NG2/CSPG4 expression in BMDMΦ, which then exhibit enhanced proliferation and decreased phagocytic capacity. These results suggest that BMDMΦ may play a much more nuanced role in secondary spinal cord injury than previously thought, including acting as early contributors to the NG2 component of the glial scar.

scholarly journals 3D inkjet printing of star block copolymer hydrogels cross-linked using various metallic ions

RSC Advances ◽  
2017 ◽  
Vol 7 (88) ◽  
pp. 55571-55576
Author(s):  
Y. Nakagawa ◽  
S. Ohta ◽  
M. Nakamura ◽  
T. Ito
Keyword(s):  
Block Copolymer ◽  
Inkjet Printing ◽  
Metallic Ions ◽  
Star Block Copolymer ◽  
First Time

We have, for the first time, investigated 3D inkjet printing of ionically cross-linked star block copolymer hydrogels.

PDGF-D activation by macrophage-derived uPA promotes AngII-induced cardiac remodeling in obese mice

2021 ◽  
Vol 218 (9) ◽  
Author(s):  
Yu-Wen Cheng ◽  
Ze-Bei Zhang ◽  
Bei-Di Lan ◽  
Jing-Rong Lin ◽  
Xiao-Hui Chen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Adipose Tissue ◽  
Cardiac Remodeling ◽  
High Fat Diet ◽  
Platelet Derived Growth Factor ◽  
Obese Mice ◽  
Mechanistic Studies ◽  
High Fat ◽  
Cardiac Damage ◽  
First Time

Obesity-induced secretory disorder of adipose tissue–derived factors is important for cardiac damage. However, whether platelet-derived growth factor-D (PDGF-D), a newly identified adipokine, regulates cardiac remodeling in angiotensin II (AngII)–infused obese mice is unclear. Here, we found obesity induced PDGF-D expression in adipose tissue as well as more severe cardiac remodeling compared with control lean mice after AngII infusion. Adipocyte-specific PDGF-D knockout attenuated hypertensive cardiac remodeling in obese mice. Consistently, adipocyte-specific PDGF-D overexpression transgenic mice (PA-Tg) showed exacerbated cardiac remodeling after AngII infusion without high-fat diet treatment. Mechanistic studies indicated that AngII-stimulated macrophages produce urokinase plasminogen activator (uPA) that activates PDGF-D by splicing full-length PDGF-D into the active PDGF-DD. Moreover, bone marrow–specific uPA knockdown decreased active PDGF-DD levels in the heart and improved cardiac remodeling in HFD hypertensive mice. Together, our data provide for the first time a new interaction pattern between macrophage and adipocyte: that macrophage-derived uPA activates adipocyte-secreted PDGF-D, which finally accelerates AngII-induced cardiac remodeling in obese mice.

Amplification of Ehrlichial DNA from Dogs 34 Months after Infection with Ehrlichia canis

1998 ◽  
Vol 36 (1) ◽  
pp. 73-76 ◽  
Author(s):  
Shimon Harrus ◽  
Trevor Waner ◽  
Itzhak Aizenberg ◽  
Janet E. Foley ◽  
Amy M. Poland ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Carrier State ◽  
Ehrlichia Canis ◽  
Beagle Dogs ◽  
Three Samples ◽  
Canine Monocytic Ehrlichiosis ◽  
Antibody Titers ◽  
Healthy Dogs ◽  
First Time ◽  
Indirect Immunofluorescent

In order to determine whether dogs in the subclinical phase of canine monocytic ehrlichiosis (CME) are carriers of Ehrlichia canis and to determine the significance of persistent indirect immunofluorescent anti-E. canis antibody titers during this phase, PCR was performed with blood, bone marrow, and splenic aspirates collected 34 months postinoculation from six clinically healthy beagle dogs experimentally infected with E. canis. At least one of the three samples (spleen, bone marrow, and blood) from four of the six dogs was PCR positive. The spleens of all four of these dogs were PCR positive, and the bone marrow and blood of two of the four dogs were PCR positive. Indirect immunofluorescent-antibody titers increased progressively during the first 5 months postinfection, remained high for an additional period of more than 11 months, and declined thereafter, suggesting that the dogs were recovering from the disease. Five of the dogs remained seropositive 34 months postinfection. The data obtained in this study demonstrate for the first time that clinically healthy dogs in the subclinical phase of CME are carriers of the rickettsia. It was shown that dogs can harbor E. canisfor years without developing the chronic clinical disease and that dogs can eliminate the parasite and recover from CME without medical treatment. Our findings suggest that the spleen is the organ most likely to harbor E. canis parasites during the subclinical phase and the last organ to accommodate the parasite before elimination. It was concluded that PCR of DNA extracted from splenic aspirates is a reliable method for determining the carrier state of CME.

Effects of spaceflight on rat erythroid parameters

1996 ◽  
Vol 81 (1) ◽  
pp. 117-122 ◽  
Author(s):  
Z. Allebban ◽  
L. A. Gibson ◽  
R. D. Lange ◽  
T. L. Jago ◽  
K. M. Strickland ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Life Sciences ◽  
Lower Number ◽  
Ground Control ◽  
Colony Forming Unit ◽  
Human Erythropoietin ◽  
Group A ◽  
Group B ◽  
First Time ◽  
Group D

Hematologic studies were performed on 21 ground control rats and 21 rats flown during the Spacelab Life Sciences-2 14-day mission. Group A (n = 5) was used to collect blood in flight and 9 days postflight, group B (n = 5) was injected with recombinant human erythropoietin (rhEpo), group C (n = 5) received saline as a control, and group D (n = 6) was killed in flight and tissues were collected. Results indicated no significant changes in peripheral blood erythroid elements between flight and ground control rats. The nonadherent bone marrow on flight day 13 showed a lower number of recombinant rat interleukin-3 (rrIL-3)-responsive and rrIL-3 + rhEpo-responsive blast-forming unit erythroid (BFU-e) colonies in flight rats compared with ground control rats. On landing day, a slight increase in the number of rhEpo + rrIL-3-responsive BFU-e colonies of flight animals compared with ground control rats was evident. Nine days postflight, bone marrow from flight rats stimulated with rhEpo alone or with rhEpo + rrIL-3 showed an increase in the number of colony-forming unit erythroid colonies and a decrease in BFU-e colonies compared with ground control rats. This is the first time that animals were injected with rhEpo and subsequently blood and tissues were collected during the spaceflight to study the regulation of erythropoiesis in microgravity.

A Critical Role for CD48 Antigen in Regulating Alloengraftment and Lymphohematopoietic Recovery After Bone Marrow Transplantation

Blood ◽  
1998 ◽  
Vol 92 (11) ◽  
pp. 4453-4463 ◽  
Author(s):  
Bruce R. Blazar ◽  
Patricia A. Taylor ◽  
Angela Panoskaltsis-Mortari ◽  
Hideo Yagita ◽  
Jonathan S. Bromberg ◽  
...  
Keyword(s):  
Bone Marrow ◽  
T Cells ◽  
Bone Marrow Transplantation ◽  
T Cell ◽  
Marrow Transplantation ◽  
Cytotoxic T Lymphocyte ◽  
Critical Role ◽  
Cell Functions ◽  
Hematopoietic Recovery ◽  
Delayed Recovery

Abstract The binding of CD2, present on T cells, to its counterreceptor CD48 facilitates adhesion, signaling, alloantigen-induced cytokine production, and cytotoxic T-lymphocyte responses. Because these T-cell functions have been implicated in graft-versus-host disease (GVHD) pathogenesis, we have analyzed the effects of the CD2:CD48 pathway on GVHD mediated by CD4+ and CD8+ T cells infused into sublethally irradiated recipients. CD4+ T-cell–mediated, and to a lesser extent, CD8+ T-cell–mediated GVHD was inhibited by CD2 + 48 monoclonal antibody (MoAb) infusion. To assess the effects of combined MoAb infusion on alloengraftment, two different alloengraftment bone marrow transplantation (BMT) models were used. In both, MoAb infusion markedly inhibited alloengraftment and hematopoietic recovery post-BMT. To determine if the adverse effects on lymphohematopoiesis in the allogeneic BMT recipients were caused by an immune or nonimmune mechanism, studies were performed in congenic BMT recipients to preclude an immune mechanism as the cause for delayed recovery post-BMT. MoAb infusion resulted in impaired lymphohematopoietic recovery in congenic BMT recipients and markedly reduced day 12 colony-forming unit–spleen formation in syngeneic BMT recipients, consistent with a nonimmune mediated mechanism. Because the spleen is a site of early hematopoietic recovery post-BMT, studies were performed using adult splenectomized syngeneic BMT recipients. MoAb infusion delayed recovery in both nonsplenectomized and splenectomized recipients post-BMT, indicating that the delayed hematopoietic recovery was not the consequence of an abnormal homing pattern of hematopoietic progenitors to the spleen early post-BMT. CD48 MoAb was necessary and sufficient for the inhibition of GVHD lethality and delayed lymphohematopoietic effects of the combined MoAb regimen. CD48 MoAb was found to induce a profound modulation of CD48 antigen expression on BM cells, suggesting that the CD48 antigen may have an important function in hematopoiesis in the BM compartment. Taken together, these data provide evidence that the CD48 antigen plays a critical role in regulating hematopoiesis in post-BMT.

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