Diamond-like Carbon Film Coating for Tissue Engineering

2008 ◽  
Vol 1138 ◽  
Author(s):  
Yasuharu Ohgoe ◽  
Haruki Matsuo ◽  
Kazuhiro Nonaka ◽  
Toshiyuki Yaguchi ◽  
Kazuya Kanasugi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Surface Composition ◽  
Umbilical Vein ◽  
Carbon Film ◽  
Biological Response ◽  
Film Coating ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
Diamond Like Carbon ◽  
Dlc Film

AbstractIn this study, we focus on effect of diamond-like carbon (DLC) coating on scaffold for tissue engineering. DLC film was deposited on segmented polyurethane (SPU) scaffold sheet which consists of micro SUP fibers. Structural and compositional effects of the DLC film coating were investigated on cell growth as an investigation of biological response. The surface composition, morphology, structures, and wettability of the DLC film coating was estimated by using X-ray photoelectron spectrometer (XPS), Scanning Electron Microscope (SEM), Ar-laser Raman spectrophotometer (Raman), and contact angle measurement. And then, human umbilical vein endothelial (HUV-EC-C) cells were grown on the DLC coated scaffold sheet. The results presented here suggest that DLC film coating is promising approach to improve biological for tissue engineering.

scholarly journals Porous Silicon Coated with Ultra-Thin Diamond-Like Carbon Film Cathodes

2001 ◽  
Vol 685 ◽  
Author(s):  
Anatoli A. Evtukh ◽  
Volodimir G. Litovchenko ◽  
Yurii M. Litvin ◽  
Dmitrii V. Fedin ◽  
Yurii V. Rassamakin ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Carbon Film ◽  
Silicon Layer ◽  
Field Enhancement ◽  
Film Coating ◽  
Layered Structures ◽  
Diamond Like Carbon ◽  
Flat Panel Display ◽  
Dlc Film ◽  
Emission Efficiency

AbstractThe main requirements to electron field emission cathodes are their efficiency, stability and uniformity. In this work we combined the properties of porous silicon layers and diamond-like carbon (DLC) film to obtain emission cathodes with improved parameters. The layered structures of porous silicon and DLC film were formed both on flat n-Si surface and silicon tips created by chemical etching. The conditions of the anodic and stain etching of silicon in HF containing solution under the illumination have been widely changed. The influence of thin (≤10nm) DLC film coating of the porous silicon layer on electron emission has been investigated. The parameters of emission efficiency such as field enhancement coefficient, effective emission areas and threshold voltages have been estimated from current-voltage dependencies to compare and characterize different layered structures. The improvement of the emission efficiency of silicon tip arrays with porous layers coated with thin DLC film has been observed. These silicon-based structures are promising for flat panel display applications.

Diamond-like Carbon Film Deposition on an Artificial Heart Blood Pump Using a Flexible Type Electrode with r.f. Plasma CVD Processing

2006 ◽  
Vol 956 ◽  
Author(s):  
Kazuya Kanasugi ◽  
Yasuharu Ohgoe ◽  
Katsuya Tsuchimoto ◽  
Keisuke Sato ◽  
Kenji K. Hirakuri ◽  
...  
Keyword(s):  
Artificial Heart ◽  
Carbon Film ◽  
Film Coating ◽  
Film Structure ◽  
Film Deposition ◽  
Blood Pump ◽  
Diamond Like Carbon ◽  
Dlc Film ◽  
Self Bias ◽  
Heart Blood

ABSTRACTDiamond-like carbon (DLC) film was deposited uniformly on an irregular structure such as a polyurethane artificial heart blood pump using a special 3-dimensional type electrode. Process of applying the DLC film coating is accomplished by inserting a large number of small metallic balls (φ0.8 mm chromium balls). It is then possible to adjust the shape of the electrode in such a way that the DLC film coating can be applied to the irregular surface of the artificial heart. In investigating the availability of the electrode, under helium (He) plasma, the plasma states were measured using double probe analysis. Lateral profiles of the electron temperature were higher in the centre and decreased towards the edges of the electrode. On the other hand, the plasma density profiles were lower in the centre part than at the edges. The electrode kept ion sheath on the artificial heart blood pump's surface at self-bias voltage uniformly. The results were that the DLC film was deposited completely on the artificial heart blood pump at the film thickness of approximately 350 - 380 nm. Additionally the film structure was uniform.

Fabrication and characterization of customized tubular scaffolds for tracheal tissue engineering by using solvent based 3D printing on predefined template

2021 ◽  
Vol 27 (2) ◽  
pp. 421-428
Author(s):  
Rudranarayan Kandi ◽  
Pulak Mohan Pandey ◽  
Misba Majood ◽  
Sujata Mohanty
Keyword(s):  
Tissue Engineering ◽  
Cell Adhesion ◽  
3D Printing ◽  
Chemical Properties ◽  
Contact Angle Measurement ◽  
Angle Measurement ◽  
In Vitro Cytotoxicity ◽  
Content Type ◽  
Tracheal Tissue

Purpose This paper aims to discuss the successful fabrication of customized tubular scaffolds for tracheal tissue engineering with a novel route using solvent-based extrusion 3D printing. Design/methodology/approach The manufacturing approach involved extrusion of polymeric ink over a rotating predefined pattern to construct customized tubular structure of polycaprolactone (PCL) and polyurethane (PU). Dimensional deviation in thickness of scaffolds were calculated for various layer thicknesses of 3D printing. Physical and chemical properties of scaffolds were investigated by scanning electron microscope (SEM), contact angle measurement, Fourier Transform Infrared Spectroscopy (FTIR) and X-ray diffraction (XRD). Mechanical characterizations were performed, and the results were compared to the reported properties of human native trachea from previous reports. Additionally, in vitro cytotoxicity of the fabricated scaffolds was studied in terms of cell proliferation, cell adhesion and hemagglutination assay. Findings The developed fabrication route was flexible and accurate by printing customized tubular scaffolds of various scales. Physiochemical results showed good miscibility of PCL/PU blend, and decrease in crystalline nature of blend with the addition of PU. Preliminary mechanical assessments illustrated comparable mechanical properties with the native human trachea. Longitudinal compression test reported outstanding strength and flexibility to maintain an unobstructed lumen, necessary for the patency. Furthermore, the scaffolds were found to be biocompatible to promote cell adhesion and proliferation from the in vitro cytotoxicity results. Practical implications The attempt can potentially meet the demand for flexible tubular scaffolds that ease the concerns such as availability of suitable organ donors. Originality/value 3D printing over accurate predefined templates to fabricate customized grafts gives novelty to the present method. Various customized scaffolds were compared with conventional cylindrical scaffold in terms of flexibility.

MULTILAYERS DIAMOND-LIKE CARBON FILM WITH GERMANIUM BUFFER LAYERS BY PULSED LASER DEPOSITION

2017 ◽  
Vol 24 (02) ◽  
pp. 1750014 ◽  
Author(s):  
Y. CHENG ◽  
Y. M. LU ◽  
Y. L. GUO ◽  
G. J. HUANG ◽  
S. Y. WANG ◽  
...  
Keyword(s):  
Critical Load ◽  
Carbon Film ◽  
High Hardness ◽  
Buffer Layers ◽  
Diamond Like Carbon ◽  
Dlc Film ◽  
Diamond Like Carbon Film ◽  
Film Hardness ◽  
Protective Films ◽  
Germanium Substrate

Multilayer diamond-like carbon film with germanium buffer layers, which was composed of several thick DLC layers and thin germanium island “layers” and named as Ge-DLC film, was prepared on the germanium substrate by ultraviolet laser. The Ge-DLC film had almost same surface roughness as the pure DLC film. Hardness of the Ge-DLC film was above 48.1[Formula: see text]GPa, which was almost the same as that of pure DLC film. Meanwhile, compared to the pure DLC film, the critical load of Ge-DLC film on the germanium substrate increased from 81.6[Formula: see text]mN to 143.8[Formula: see text]mN. Moreover, Ge-DLC film on germanium substrates had no change after fastness tests. The results showed that Ge-DLC film not only kept high hardness but also had higher critical load than that of pure DLC film. Therefore, it could be used as practical protective films.

Study on the Preparation of Silver Nanoparticles Coated with Diamond-Like Carbon Film and their Properties

2013 ◽  
Vol 745-746 ◽  
pp. 60-65
Author(s):  
Nan Yu Ma ◽  
Dan Zeng ◽  
Yu Jie Huang ◽  
Jun Wei Di ◽  
Mu Sen Li
Keyword(s):  
Deposition Time ◽  
Near Infrared ◽  
Resonance Effect ◽  
Carbon Film ◽  
Chemical Vapor ◽  
Diamond Like Carbon ◽  
Ag Nps ◽  
Surface Plasma Resonance ◽  
Dlc Film ◽  
Biological Compatibility

Ag nanoparticles (NPs) have prominent local surface plasma resonance effect (LSPR), and Ag NPs exhibit sharpest and strongest bands among all metals. Diamond-like carbon (DLC) film have good biological compatibility and also have high transmissibility in the visible and near-infrared region. A new LSPR interface between Ag NPs and ultra-thin DLC film was formed by Plasma Enhanced Chemical Vapor Deposition. The morphologies and properties of the Ag NPs coated with DLC film were studied with SEM and AFM. The results indicated that the thickness of DLC film increased with the deposition time. LSPR peak became sharper after depositing for 1 or 2 min. DLC film was prior to nucleate on the surface of Ag NPs, and it has high content of sp2 bonds near the interface. The sensitivity of new LSPR interface deposited for 20s was about the half of the sensitivity of bare Ag NPs and the sensitivity significantly decreased with deposition time. This result is helpful to understand the behavior of the new LSPR interface and to improve its sensitivity.

Deposition of a Diamond-Like-Carbon Film by Ion Plating and Investigation on its Adhesiveness

2017 ◽  
Vol 749 ◽  
pp. 70-75
Author(s):  
Xia Zhu ◽  
Kazuki Kubo ◽  
Hiromichi Toyota ◽  
Shinfuku Nomura ◽  
Yukiharu Iwamoto ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Diamond Crystal ◽  
Carbon Film ◽  
Pure Metal ◽  
Diamond Like Carbon ◽  
Interlayer Thickness ◽  
Coating Materials ◽  
Dlc Film ◽  
Ion Plating ◽  
Excellent Method

Diamond-like-carbon (DLC) films are promising as coating materials. Ion plating, an excellent method in terms of adhesiveness, step coverage, and deposition rate, can form not only pure metal films but also oxide films, nitride films, and carbonized films. In this study, which aimed to form a DLC film with good adhesiveness and a diamond crystal structure, a DLC film, with a SiC interlayer formed by ion plating with introduction of tetramethylsilane (TMS), was formed. It was experimentally revealed that as the interlayer thickness increases, the crystal structure in the DLC film becomes more diamond rich, and the adhesiveness of the DLC film and substrate is thereby improved.

scholarly journals Elements-Added Diamond-Like Carbon Film for Biomedical Applications

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Narin Sunthornpan ◽  
Shuichi Watanabe ◽  
Nutthanun Moolsradoo
Keyword(s):  
Surface Roughness ◽  
Contact Angle ◽  
Friction Coefficient ◽  
Biomedical Applications ◽  
Carbon Film ◽  
Diamond Like Carbon ◽  
Cytotoxicity Test ◽  
Aisi 316L ◽  
Gaseous Mixtures ◽  
Dlc Film

Elements-added diamond-like carbon films for biomedical applications were investigated. The aim of this work was to study the effects of the elemental contents (silicon and silicon-nitrogen) in a DLC film on its properties for biomedical applications. Pure DLC, Si-DLC, and Si-N-DLC films were prepared from C2H2, C2H2 : TMS, and C2H2 : TMS : N2 gaseous mixtures, deposited on an AISI 316L substrate using the plasma-based ion implantation (PBII) technique. The structure of films was analyzed using Raman spectroscopy. The chemical composition of films was measured using energy dispersive X-ray spectroscopy (EDS). The average surface roughness of films was measured by using a surface roughness tester. The hardness and elastic modulus of films were measured by using a nanoindentation hardness tester. The friction coefficient of films was determined using a ball-on-disk tribometer. The surface contact angle was measured by a contact angle measurement. The corrosion performance of each specimen was measured using potentiodynamic polarization. The biocompatibility property of films was conducted using the MTT assay cytotoxicity test. The results indicate that the Si-N-DLC film shows the best hardness and friction coefficient (34.05 GPa and 0.13, respectively) with a nitrogen content of 0.5 at.%N, while the Si-DLC film with silicon content of 14.2 at.%Si reports the best contact angle and corrosion potential (92.47  and 0.398 V, respectively). The Si-N-DLC film shows the highest cell viability percentage of 81.96%, which is lower than the uncoated AISI 316L; this is a considerable improvement. All specimens do not demonstrate any cytotoxicity with approximate viabilities between 74% and 107%, indicating good biocompatibilities.

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