scholarly journals GROWTH AND EXPRESSION PATTERN OF GROWTH-RELATED GENES IN THE FAST-GROWING GIANT GOURAMI Osphronemus goramy

2021 ◽  
Vol 16 (2) ◽  
pp. 79
Author(s):  
Siska Aliyas Sandra ◽  
Hasan Nasrullah ◽  
Harton Arfah ◽  
Muhammad Zairin Jr. ◽  
Alimuddin Alimuddin
Keyword(s):  
Gene Expression ◽  
Growth Traits ◽  
Fish Growth ◽  
Gene Expressions ◽  
Fast Growing ◽  
Expression Studies ◽  
Significant Difference ◽  
Molecular Assisted Selection ◽  
Growth Problem ◽  
Molecular Expression

Growth improvement of the giant gourami through molecular assisted selection offers a breakthrough solution regarding the slow growth problem in culturing the fish species. However, gene molecular expression studies and gene mapping information are scarce for this species. This study aimed to evaluate the growth, expression of the growth-related genes and compare the gene expressions between fast-growing (FG) and slow-growing (SG) fish. The polymorphism screenings were also conducted within the GH sequence of the FG and SG populations. Fish growth was analyzed by measuring length and weight once a month. The expression levels of GH, IGF1, AMPK, ARS-I, ALT, and AST genes were analyzed using real-time PCR. Twenty-five days old fish were reared for 30 days. The fish were continuously reared separately based on their body weight (BW) for 85 days until reaching 140 days old. At the end of the rearing period, the BW growth rate of the FG population was 1.569-fold higher, and body length (BL) growth was 1.056-fold higher than the SG population. FG fish have higher gene expression than the SG fish, indicating the important role of gene expression in fish growth. The polymorphisms screening within the GH sequences showed no significant difference between FG and SG fish of giant gourami. These research results provide valuable information in developing the marker-assisted selection for growth traits in giant gourami.

scholarly journals EXPRESSION OF micro RNAs (miRNAs) IN MYELODYSPLASTIC SYNDROME (MDS)

2019 ◽  
Vol 141 (7-8) ◽  
pp. 226-232
Keyword(s):  
Gene Expression ◽  
Myelodysplastic Syndrome ◽  
Healthy Volunteers ◽  
Myelogenous Leukemia ◽  
University Hospital ◽  
Gene Expressions ◽  
Hematopoietic Stem ◽  
Increased Risk ◽  
Micro Rnas ◽  
Significant Difference

Myelodisplasia or myelodysplastic syndrome (MDS) is the name for a group of heterogeneous clonal hematological disorders of hematopoietic stem cells followed by ineffective hematopoesis of one or more cell lines and the emergence of consequent cytopenias with increased risk of progression to acute myelogenous leukemia (AML). Micro Messenger Ribonucleic Acids (miRNAs) are short, non-coding RNA molecules that, apart from contributing to MDS pathogenesis, act as regulators of epigenetic mechanisms and also are recognized as potential prognostic markers for early diagnosis and classification of MDS. The aim of the study was to examine the levels of gene expression of specific miRNAs (hsa-miR-125a, hsa-miR-99b, hsa-miR-126 and hsa-miR-125b) in healthy volunteers plasma and MDS diagnosed patients. Gene expressions of miRNAs were determined at the Clinical Institute of Medical Biochemistry and Laboratory Medicine, Merkur University Hospital, accredited according to EN ISO 15189:2012, in plasma samples of four healthy volunteers and 33 MDS patients diagnosed at the Institute of Hematology of the Clinic for Internal Diseases of Merkur University Hospital, Reference Center of the Ministry of Health of the Republic of Croatia for Diagnosis and Treatment of MDS. Statistically significant difference in gene expression of miRNA in healthy volunteers compared to the MDS patients was not found (P [hsa-miR-125a] = 0.398; P [hsa-miR-99b] = 0.134; P [hsa- miR-126] = 0.305; P [hsa-miR-125b] = 0.079). MiRNA ratios of hsa-miR-125a and hsa-miR-99b in MDS patients were almost twice as high compared to normalized levels of gene expression in healthy volunteers (2.30 versus 1.90), and the level of change of miRNAs hsa-miR-125 and hsa-miR-99b was more than two times higher than the level of change of miRNA hsa-miR-125b. Finally, the results of the research indicate that the gene expression of miRNAs hsa-miR-125a and hsa-miR-99b could be regulated by the same mechanism and could be clinically relevant in MDS patients.

Role of Non-PTS dependent glucose permease (GlcU) in maintaining the fitness cost during acquisition of nisin-resistance by Enterococcus faecalis

2019 ◽  
Author(s):  
Sandeep Kumar ◽  
Kapil Singh Narayan ◽  
Shruti Shandilya ◽  
Shiv Kumar Sood ◽  
Suman Kapila
Keyword(s):  
Gene Expression ◽  
Glucose Uptake ◽  
Buffalo Milk ◽  
Food Preservation ◽  
Sensitive Strain ◽  
Fitness Cost ◽  
Expression Studies ◽  
Significant Difference ◽  
Gene Expression Studies

ABSTRACT Nisin is used for food preservation due to its antibacterial activity. However, some bacteria survive under the prevailing conditions owing to the acquisition of resistance. This study aimed to characterize nisin-resistant E. faecalis isolated from raw buffalo milk and investigate their fitness cost. FE-SEM, biofilm and cytochrome-c assay were used for characterization. Growth kinetics, HPLC, qPCR, and western-blotting were performed to confer their fitness cost. Results revealed that nisin-resistant E. faecalis were morphologically different from sensitive strain and internalize more glucose. However, no significant difference was observed in the growth pattern of the resistant strain compared to that of the sensitive strain. A non-phosphotransferase glucose permease (GlcU) was found to be associated with enhanced glucose uptake. Conversely, Mpt, a major phospho-transferase system responsible for glucose uptake, did not play any role, as confirmed by gene expression studies and western blot analysis of HPr protein. The phosphorylation of His-15 residue of HPr phosphoprotein was reduced, while that of the Ser-46 residue increased with progression in nisin-resistance, indicating that it may be involved in the regulation of pathogenicity. In conclusion, resistance imposes a significant fitness cost and GlcU plays a key role in maintaining the fitness cost in nisin-resistant variants.

scholarly journals Evaluation of the quality of RNA extracted from archival FFPE glioblastoma and epilepsy surgical samples for gene expression assays

2018 ◽  
Vol 71 (8) ◽  
pp. 695-701 ◽  
Author(s):  
Harry R Haynes ◽  
Clare L Killick-Cole ◽  
Kelly M Hares ◽  
Juliana Redondo ◽  
Kevin C Kemp ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reference Gene ◽  
Ffpe Tissue ◽  
Tissue Samples ◽  
Total Rna ◽  
Expression Studies ◽  
Significant Difference ◽  
Gene Expression Analyses ◽  
Gene Expression Studies ◽  
And Control

AimsHistopathological tissue samples are being increasingly used as sources of nucleic acids in molecular pathology translational research. This study investigated the suitability of glioblastoma and control central nervous system (CNS) formalin-fixed paraffin embedded (FFPE) tissue-derived RNA for gene expression analyses.MethodsTotal RNA was extracted from control (temporal lobe resection tissue) and glioblastoma FFPE tissue samples. RNA purity (260/280 ratios) was determined and RNA integrity number (RIN) analysis was performed. RNA was subsequently used for RT-qPCR for two reference genes,18SandGAPDH.ResultsReference gene expression was equivalent between control and glioblastoma tissue when using RNA extracted from FFPE tissue, which has key implications for biological normalisation for CNS gene expression studies. There was a significant difference between the mean RIN values of control and glioblastoma FFPE tissue. There was no significant correlation between 260/280 or RIN values versus total RNA yield. The age of the tissue blocks did not influence RNA yield, fragmentation or purity. There was no significant correlation between RIN or 260/280 ratios and mean qPCR cycle threshold for either reference gene.ConclusionsThis study showed that routinely available CNS FFPE tissue is suitable for RNA extraction and downstream gene expression studies, even after 60 months of storage. Substantial RNA fragmentation associated with glioblastoma and control FFPE tissue blocks did not preclude downstream RT-qPCR gene expression analyses. Cross validation with both archival and prospectively collated FFPE specimens is required to further demonstrate that CNS tissue blocks can be used in novel translational molecular biomarker studies.

scholarly journals Vitamin D and Vitamin D Receptor in Scleroderma Subtypes

2020 ◽  
pp. 19-24
Author(s):  
Kocak Ayse
Keyword(s):  
Gene Expression ◽  
Vitamin D ◽  
Vitamin D Receptor ◽  
Mrna Levels ◽  
Diffuse Type ◽  
Gene Expressions ◽  
Vdr Gene ◽  
Vitamin D Levels ◽  
Significant Difference ◽  
Tgf Β1

Vitamin D Receptor (VDR) is a member of the nuclear hormone receptor family. 1,25(OH)2D, a form of metabolically active vitamin D3 form, is the ligand of VDR. When VDR and 1,25(OH)2D are connected, many genes start to molecular interaction reactions that will modulate the transcription. VDR has been shown to be a negative regulator of the transforming growth factor beta-1 / Smad (TGF-β1 / Smad) signalling pathway. TGF-β1 / Smad signalling is important in the pathogenesis of scleroderma (SSc). Vitamin D has pleiotropic effects including immunomodulatory and antifibrotic properties in scleroderma pathogenesis. The aim of this study was to investigate the expression of VDR and the levels of vitamin D in scleroderma subtypes and study the possible correlation between the two parameters. 28 SSc patients and 30 healthy controls were included in the study and they were classified according to the 2013 ACR / EULAR criteria and Rodnan Scores were calculated. 14 were of the limited type and 14 were of the diffuse type of scleroderma. Vitamin D levels were determined in serum. Vitamin D level was measured by chemiluminescence immunometric assay. VDR gene expression was determined by quantitative PCR in isolated RNAs from the blood. Changes in mRNA levels were analysed and beta-actin was used as the housekeeping gene. Also, TGF-β1 gene expressions were determined. VDR gene expressions in diffuse type scleroderma patients were significantly decreased compared to the control. TGF-β1 gene expressions were increased in diffuse type scleroderma. It was found that VDR gene expression in limited type scleroderma patients did not show any significant difference when compared to control. Vitamin D levels and VDR gene expressions showed no correlation in scleroderma subtypes. VDR gene expression decreased in patients with diffuse type scleroderma and showed negative correlation with the Rodnan score and TGF-β1 gene expressions. There was no significant difference between vitamin D and VDR levels.

scholarly journals Effects of Developmental Arsenic Exposure on the Social Behavior and Related Gene Expression in C3H Adult Male Mice

2019 ◽  
Vol 16 (2) ◽  
pp. 174 ◽  
Author(s):  
Soe-Minn Htway ◽  
Mya-Thanda Sein ◽  
Keiko Nohara ◽  
Tin-Tin Win-Shwe
Keyword(s):  
Gene Expression ◽  
Social Behavior ◽  
Adult Male ◽  
Arsenic Exposure ◽  
Receptor Expression ◽  
Heme Oxygenase 1 ◽  
Male Mice ◽  
Related Gene ◽  
Gene Expressions ◽  
Significant Difference

Arsenic is carcinogenic and teratogenic. In addition, it is also a developmental neurotoxicant. Little is known however about the effect of arsenic exposure during brain development on social behavior. This study aimed to detect the effect of developmental arsenic exposure on social behavior and related gene expression in C3H adult male mice. Pregnant C3H mice were exposed to sodium arsenite (NaAsO2, 85 ppm in the drinking water) from gestational day (GD) 8 to 18. The F1 generation male pups from different mothers were taken and social behavior tasks were examined. Social behavioral-related gene expression in the prefrontal cortex was determined by the real-time RT-PCR method. The mice with developmental arsenic exposure showed poor sociability and poor social novelty preference. Glutamate receptor expression (NMDA and AMPA receptor subunits) showed no significant difference, but gene expressions of serotonin receptor 5B (5-HT 5B) and brain-derived neurotrophic factor (BDNF) were significantly decreased (p < 0.05) in the arsenic-exposed group compared to control group. The heme oxygenase-1 (HO-1) and cyclooxygenase-2 (COX-2) gene expressions were not significantly different. Our findings indicate that developmental arsenic exposure might affect social behavior by modulating serotonin receptors and reducing BDNF. Some oxidative stress markers and inflammatory markers were not affected.

The prognostic value of gene expressions of ERCC1 and RRM1 in non-small cell lung cancer.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e19071-e19071
Author(s):  
Serap Hasturk ◽  
Ozlem Olgunus ◽  
Abdullah Tuli ◽  
Ebru Dundar ◽  
Emine Ozgur Unlu
Keyword(s):  
Gene Expression ◽  
Lung Cancer ◽  
Survival Analysis ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Small Cell ◽  
Advanced Stage ◽  
Small Cell Lung ◽  
Gene Expressions ◽  
Significant Difference

e19071 Background: Non small cell lung cancer is the most common cause of death due to cancer in the world. The role of ERCC1 and RRM1 genes has been researched in prediction of lung cancer prognosis and response of patients to chemotheraphy. We aimed to evaluate the effects of ERCC1 and RRM1 gene expressions profiles on the prediction of the prognosis and the treatment on bronchoscopy specimens of advanced stage NSCLC patients. Methods: The levels of ERCC1 and RRM1 gene expressions were studied for 76 patients which were diagnosed by bronchoscopic biopsy parafin embedded tissue samples. The RRM1 and ERCC1 gene expression profiles were examined by RT-PCR method. Forty were diagnosed with squamous cell cancer, 24 with adenocarcinoma, 12 with NOS NSCLC. Thirty patients were chemotherapy native, 33 were received gemcitabine-cisplatin and 13 were received docetaxel- cisplatin doublets chemotherapy. Results: The mean age of the patients in the study was 59.8±9.3 (years ± SD). The levels of gene expressions of ERCC1 and RRM1 were found to be 2-9 (median 4.9), and 1.3-17.7 (median 6.8). No significant difference was detected between ERCC1 and RRM1 levels and age, gender, histologic type, ECOG, weight loss when the median gene expression levels were used as cut off value. Also, no significant difference was observed for survival analysis among the patients that have low ERCC1 mRNA level (p=0.41). The level of gene expression of ERCC1 was lower in patients with advanced stage (p=0.06). In addition, no significant difference was detected for the survival analysis within the patients that have the low and high RRM1 mRNA levels (p=0.43). When the survival analyses were evaluated between the patients who had chemotherapy and the ones who did not; no significant difference was detected according to ERCC1 and RRM1 levels. Conclusions: We conclude that the analysis of ERCC1 and RRM1 gene expression profiles based on bronchoscopy obtained samples appeares feasible, but the methodology and cut off points that was used to classify expression level as ‘high’ or ‘low’ would require further optimization. These two gene signature proposed for advanced stage NSCLC may not be useful, particularly not ready for clinical application.

scholarly journals Dry Needling of a Healthy Rat Achilles Tendon Increases Its Gene Expressions: A Pilot Study

Pain Medicine ◽  
2020 ◽  
Author(s):  
Laura Calderón-Díez ◽  
José Luis Sánchez-Sánchez ◽  
Javier Herrero-Turrión ◽  
Joshua Cleland ◽  
José L Arias-Buría ◽  
...  
Keyword(s):  
Gene Expression ◽  
Pilot Study ◽  
Achilles Tendon ◽  
Intervention Group ◽  
Male Rats ◽  
Sprague Dawley ◽  
Dry Needling ◽  
Gene Expressions ◽  
Molecular Expression ◽  
Hematoxylin Eosin

Abstract Background Tendon dry needling is a potential treatment for tendinopathies. Several hypotheses have been proposed to explain its underlying mechanisms. No studies (to the best of our knowledge) have investigated changes in gene expression. Objective To investigate histological and gene expression changes after the application of dry needling to the healthy Achilles tendons of rats. Methods Six Sprague-Dawley male rats were randomly divided into two groups: no intervention or dry needling. Dry needling consisted of three sessions (once per week) to the Achilles tendon. Molecular expression of several genes involved in tendon repair and remodeling (e.g., Cox2, Mmp2, Mmp9, Col1a1, Col3a1, Vefg, and Scx) was assessed 7 days after the last needling session (day 28) or 28 days after for the no-intervention group. Histological tissue changes were determined with hematoxylin-eosin analyses. Results The hematoxylin-eosin–stained images revealed no substantial differences in collagen structure or the presence of inflammatory cells between the dry needling and no-intervention groups. A significant increase in the molecular expression of Cox2, Mmp2, Col3a1, and Scx genes was observed in Achilles tendons treated with dry needling when compared with the no-intervention group. Conclusion This animal pilot study found that the application of dry needling to the healthy Achilles tendons of rats is able to increase the expression of genes associated with collagen regeneration and tissue remodeling of the extracellular matrix with no further histological damage to the tendon.

scholarly journals Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle – an Experimental Study on a Porcine Model

2010 ◽  
Vol 79 (3) ◽  
pp. 453-457
Author(s):  
Petr Hýža ◽  
Libor Streit ◽  
Eduard Gopfert ◽  
Daniel Schwarz ◽  
Michal Masařík ◽  
...  
Keyword(s):  
Gene Expression ◽  
Target Genes ◽  
Pedicled Flap ◽  
Control Group ◽  
Peripheral Blood Flow ◽  
Gene Expressions ◽  
Endothelin 1 ◽  
Significant Difference ◽  
Group A ◽  
Relative Gene

The aim of this study was to evaluate the amount of Endothelin-1 (ET-1) gene expression in the vasospastic vessel of the flap pedicle to prove or disprove the role of ET-1 gene expression in pathogenesis of mechanically induced vasospasm. The vasospasm was induced by the tension on the pedicle of the pedicled caudal superficial epigastric flap on 8 pigs. Laser Doppler was used for peripheral blood flow measurement. Specimens from the vasospastic vessel (group of specimens B) and from the flap border with no vasospasm (control group A) were taken 2 h after the stimulus initiation. Detection of ET-1 mRNA by Quantitative Real-Time RT-PCR was performed. β-actin was selected as an acceptable reference gene. Relative gene expression data were given as the n-fold change in transcription of target genes normalized to the endogenous control. Relative gene expressions and time indicators of vasospasm were compared in both groups. No significant difference of the ET-1 gene expressions was found between groups A and B (p = 0.505). No correlation between the duration of vasospasm and ET-1 gene expression was found as well (p = 0.299). In conclusion, the expression of the ET-1 gene in the mechanically induced vasospastic vessel of the pedicled flap was not significantly increased. In this study, the causality of the vasospasm pathogenesis and gene expression of ET-1 was not proven.

scholarly journals Determination of PFOR gene expression in strains of G. intestinalis with different inhibitory concentrations of metronidazole

2015 ◽  
Vol 9 (05) ◽  
pp. 519-523 ◽  
Author(s):  
Rozalia Begaydarova ◽  
Yekaterina Yukhnevich ◽  
Dmitry Babenko ◽  
Sholpan Kaliyeva ◽  
Iliya Azizov ◽  
...  
Keyword(s):  
Gene Expression ◽  
Software Tool ◽  
Giardia Intestinalis ◽  
Gene Expressions ◽  
Development Of Resistance ◽  
Metronidazole Resistance ◽  
Significant Difference ◽  
The Difference ◽  
Specific Pair

Introduction: Giardia intestinalis is the most important and common diarrhea-causing parasitic protozoa worldwide with growing clinical relevance in public health. There are many documented cases of G. intestinalis resistance to metronidazole (MZ). Pyruvate: ferredoxin oxidoreductase (PFOR), the membrane-localized enzyme, plays a key role in the development of resistance to drugs. The aim of the present study was to evaluate the difference in the levels of PFOR gene expression between MZ-resistant and MZ-susceptible strains of G. intestinatlis. Methodology: From 159 samples with G. intestinalis cysts, 48 strains were successfully cultivated. Using specific pair primers, PFOR gene expressions were estimated in different groups of Giardia. The polymerase chain reaction (PCR) data were analyzed with Bayesian analysis of qRT-PCR data using MCMC.qpcr package, with relative expression software tool (REST) and quantitative PCR CopyCount web source. Results: In the group of Giardia with minimum inhibitory concentration (MIC) of 6.3 µM, the level of PFOR gene expression was downregulated and compared with controls, differed by 1.5 to 2.8 times. At the same time, there was no significant difference in PFOR gene expression between the control (susceptible) group and the group with MIC of 3.2 µM. Conclusions: Though there is association between PFOR gene expression and metronidazole resistance of Giardia intestinalis, the level of PFOR gene expression cannot be a strong genetic marker to predict level of resistance to metronidazole based on MICs.

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