scholarly journals Fractionation of phenolic compounds from Sonneratia apetala pneumatophores and their bioactivities

2017 ◽  
Vol 39 (4) ◽  
pp. 451-456 ◽  
Author(s):  
Nguyen Sao Mai ◽  
Dao Van Tan

Sonneratia apetala pneumatophore samples were collected from Xuan Thuy National Park, and then 80% methanol extract was fractionated through Sephadex LH-20 column chromatography. After elution with 95% ethanol and 50% acetone, two phenolic fractions were obtained from the crude extract of of S. apetala pneumatophore. The acetone fraction consisted of tannins having high antioxidant activity, 1.14 times higher than that of ascorbic acid. This fraction also have higher activity of α-amylase inhibition (IC50 0.83 mg/mL) and R. sativus root growth inhibition (IC50 6.4 mg/mL) than did the ethanol fraction. In contrast, ethanol fraction consisted of low-molecular phenolic compounds having inhibitory effects on Samonella tiphimurium growth. Such antibacterial activity was not observed in the acetone extract. Citation: Nguyen Sao Mai, Dao Van Tan, 2017. Fractionation of phenolic compounds from Sonneratia apetala pneumatophores and their bioactivities. Tap chi Sinh hoc, 39(4): 451-456. DOI: 10.15625/0866-7160/v39n4.10708.*Corresponding author: [email protected] 19 September 2017, accepted 12 December 2017

2019 ◽  
Vol 11 (1) ◽  
pp. 56-65
Author(s):  
Daniele Martins Firmiano ◽  
Gabriela Marques Bittencourt ◽  
Alessandra Lopes de Oliveira

Objective: This research used the Pressurized Liquid Extraction process (PLE) to obtain cambuci extracts rich in phenolic compounds and, consequently, with high antioxidant activity. Methods: We determined the centesimal composition and the mean particle size of the dried and crushed sample for characterization of the fixed bed extractor. Extractions with pressurized ethanol were performed following a Central Composite Design (CCD) with temperature (60 °C at the center point with ± 10 °C amplitude) and the contact time between the solvent and the matrix (6 min at the center point with ± 2 min amplitude) as independent variables in the process. The Total Phenolic Content (TPC) of compounds and antioxidant activity were determined. Results: The mean particle size of the cambuci in the PLE was 263.4 μm with apparent and real densities of 32.42 g/cm3 and 1.62 g/cm3, respectively, resulting in a fixed bed porosity of 0.43. The centesimal composition of the comminuted and dried sample was 12.5 ± 0.1% moisture, 3.2 ± 0.2% ash, 3.35 ± 0.07% crude protein, 10.32 ± 0.08% crude fiber and 0.35 ± 0.04% ethereal extract. The highest extraction yield (41.7%) was obtained by using the binomial 70 ºC with 8 min of solvent contact in three batches. Extracts with the highest TPC (6501.10 mg AGE/100 g sample) were obtained by the binomial 74 °C with 6 min of contact; the binomial 50 °C with 4 min of contact gave rise to the extract with the highest antioxidant activity. The CCD showed high yield extracts with high antioxidant activity and a high total phenolic compound content. Conclusion: With the use of the proposed experimental design, it was possible to optimize the extraction of total phenolic compounds from the sample.


2016 ◽  
Vol 37 (4) ◽  
Author(s):  
Hoang Thi Yen ◽  
Trinh Thi Thuy Linh ◽  
Mai Chi Thanh ◽  
Nguyen Thi Thu Huyen ◽  
Lai Thi Ngoc Ha ◽  
...  

2018 ◽  
Vol 11 (13) ◽  
pp. 114 ◽  
Author(s):  
Misri Yanty Lubis ◽  
Lamek Marpaung ◽  
Muhammad Pandapotan Nasution ◽  
Partomuan Simanjuntak

 Objective: This study was aimed to isolate and investigate antioxidant activity of gallic acid in pods of jiringa (Archidendron jiringa [Jack] I.C. Nielsen).Methods: Pods of jiringa were extracted by maceration. Phenolic compounds were tested using FeCl3. Identification of pure compound was obtained from spectra data from nuclear magnetic resonance of proton H (1H NMR), NMR of carbon (13C NMR), infra-red, and mass spectrometry. Antioxidant activity was investigated using 1,1-diphenyl-2-picrylhydrazyl method.Results: The IC50 of gallic acid from jiringa’s pods (A. jiringa [Jack] I.C. Nielsen) was 3.65 μg/ml. This value showed that gallic acid from jiringa’s pods (A. jiringa [Jack] I.C. Nielsen) had high antioxidant activity.Conclusions: Gallic acid presents in pods of jiringa (A. jiringa [Jack] I.C. Nielsen) and has high antioxidant activity.


Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1167
Author(s):  
Marek Szołtysik ◽  
Alicja Z. Kucharska ◽  
Anna Sokół-Łętowska ◽  
Anna Dąbrowska ◽  
Łukasz Bobak ◽  
...  

The aim of the study was to evaluate the effect of purified extract from Rosa spinosissima fruits on the quality characteristics and antioxidant properties of yoghurt. The extract, added to yoghurt at a concentration of 0.1% and 0.2%, contained high quantities of phenolic compounds and exhibited high antioxidant activity due to the presence of anthocyanins flavan-3-ols, flavonols and ellagitannins. Yoghurt physicochemical properties, microbiology and antioxidant properties were evaluated after 1, 7 and 14 days of storage at a temperature of 4 °C. The data revealed a positive influence of rose preparation on yoghurt’s microflora and on its other properties. The highest count of traditional yoghurt microflora was observed in samples with 0.2% of extract. Its addition had a positive effect on the yoghurts’ color, giving them a characteristic pink color of an intensity dependent on additive concentration. It also significantly affected the yoghurts’ antioxidant properties, which were stable during storage, as well as the content of the introduced phenolic compounds.


LWT ◽  
2018 ◽  
Vol 89 ◽  
pp. 284-290 ◽  
Author(s):  
Chloe D. Goldsmith ◽  
Quan V. Vuong ◽  
Costas E. Stathopoulos ◽  
Paul D. Roach ◽  
Christopher J. Scarlett

Author(s):  
Habchi A. ◽  
Dekmouche M. ◽  
Hamia C. ◽  
Saidi M. ◽  
Yousfi M. ◽  
...  

This study was designed to evaluate the antioxidant activity of phenolic compounds for six cultivars of date from In Saleh region (Phoenix dactylifera L.), which were extracted by reflux with solvents, 80% methanol and 70% aceton after the phenolic and flavonoid content were estimated. The total phenolic content (TPC) and total flavonoid content (TFC) of the extracts were measured using Folin–Ciocalteau and aluminum chloride colorimetric methods, respectively. Antioxidant activity acetone/H2O and methanol/H2O extracts using ABTS, DPPH, FRAP, TAC and CUPRAC assays. The results showed that methanolic solvent reflux gives better yields. The highest level of phenolic compounds for all dates varieties was found in the 80% methanol extract. This level ranged from 8.04 ± 0.14 to 46.81 ± 1.22 mg GAE/100 g fresh weight and flavonoid content was observed relatively low for both two solvents. However, extraction into 70% acetone gave the highest antioxidant activity with all assay. These results showed that dates had strongly scavenging activity on DPPH reaching 3.12 µg/mL in methanol/H2O from Cul1 (6.9 µg/mL with ascorbic acid), also on ABTS reaching 6.51 µg/mL in acetone/H2O from Cul2 (104.98 µg/mL with ascorbic acid).


2016 ◽  
Vol 46 (2) ◽  
pp. 228-236 ◽  
Author(s):  
Neuza Jorge ◽  
Carolina Médici Veronezi ◽  
Danusa Cassiano Pereira

Purpose – This study aims to deal with the evaluation of the antioxidant capacity of lyophilized hydroalcoholic extracts of red peppers in natura. Furthermore, preference was evaluated for the taste and color of soybean oil added red pepper extracts. Design/methodology/approach – The antioxidant capacity was determined by four methods. The content of phenolic compounds, carotenoids and ascorbic acid in the extracts was determined by chromatographic, spectrophotometric and titration methods, respectively. Findings – The results showed that the highest antioxidant capacity was found in Malagueta pepper extract through reducing power (FRAP) method. In this same extract, high amount of phenolic compounds was found. However, the extracts of Bode and Dedo-de-moça peppers had higher amounts of carotenoids and ascorbic acid, respectively. Sensorially, the oil added extracts were preferred. Practical implications – Red peppers are very popular and consumed worldwide, besides being constituted of important phytochemicals. Results showed high antioxidant activity in the extracts of peppers, and high content of phenolic compounds, carotenoids and ascorbic acid mainly in chili. This study highlights the importance of the extracts of red peppers, genus Capsicum, as a source of antioxidants, in addition to vegetable oils. Originality/value – It is important to check the acceptance of the application of extract in vegetable oil, so it can be marketed as a natural antioxidant. This study provides valuable information about the antioxidant capacity of extracts of red peppers and its acceptance.


2016 ◽  
Vol 19 (2) ◽  
pp. 147-151 ◽  
Author(s):  
Md Emdadul Hasan Mukul ◽  
Mohammad Salim Hossain ◽  
Sayed Koushik Ahamed ◽  
Pankaj Debnath ◽  
Mariyam Akter

This study was carried out to investigate the antioxidant activity in terms of free radical scavenging capacity and membrane stabilizing ability of methanol extract of bark of Sonneratia apetala. The total phenol content of the extract was measured by Folin-Ciocalteu reagent. The antioxidant potential was investigated using 2,2 diphenylpicrylhydrazyl (DPPH), reducing power assay and chelating power determination. The membrane stabilizing capacity was assessed by monitoring the hypotonic solution - and heat-induced haemolysis of human erythrocytes. The total phenolic content was found 50.75 mg/gm of gallic acid equivalent. The extract exhibited significant antioxidant activity in DPPH free radical scavenging assay with IC50 value of 81.42 µg/ml as compared to the standard, BHT (IC50=42.56 µg/ml), Fe2+ ion reducing power assay and chelating power ability. In case of assay for chelating ability, EDTA represented 98.76% chelation while the plant extract showed 82.83% at concentration of 100 ?g/ml. In addition, the methanol extract of bark of this plant was found to moderately inhibit the haemolysis of human erythrocyte. In conclusion, the methanol extract of S. apetala could be valuable candidate for future development for antioxidant activity.Bangladesh Pharmaceutical Journal 19(2): 147-151, 2016


2014 ◽  
Vol 16 (1) ◽  
pp. 41-46 ◽  
Author(s):  
R. Scherer ◽  
HT. Godoy

The effect of extraction methods and solvents on overall yield, total phenolic content, antioxidant activity, and the composition of the phenolic compounds in Xanthium strumarium extracts were studied. The antioxidant activity was determined by using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), and the composition of the phenolic compounds was determined by HPLC-DAD and LC/MS. All results were affected by the extraction method, especially by the solvent used, and the best results were obtained with the methanol extract. The methanolic and ethanolic extracts exhibited strong antioxidant activity, and the chlorogenic and ferulic acids were the most abundant phenolic compounds in the extracts.


Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2561
Author(s):  
Andrzej Cendrowski ◽  
Marcin Królak ◽  
Stanisław Kalisz

The aim of the present study was to determine the influence of the winemaking process on the antioxidant potential and content of phenolic compounds and L-ascorbic acid in wines from the fruits of Rosa rugosa. The results obtained in this study clearly indicate that the fruits of the Rosa rugosa are a desirable raw material for the production of fruit wine. The parameters of the technological process of producing wines from rose fruits had a diversified influence on the tested quality characteristics. Aged wines contained phenolics levels of 473–958 mg/100 mL GAE. The final concentrations of ascorbic acid ranged from 61 to 155 mg/100 mL for the different variants of the wine. Wines revealed high antioxidant activity in assay with DPPH. On the basis of the obtained results, it can be assumed that all the applied variants of the winemaking process are suitable for rose fruit wine. Each variant ensured at least the stability of the antioxidant capacity.


Sign in / Sign up

Export Citation Format

Share Document