Effects of micro-environmental factors on the photoautotrophic growth of Hibiscus sagittifolius Kurz cultured in vitro

2018 ◽  
Vol 39 (4) ◽  
pp. 496-506 ◽  
Author(s):  
Nguyen Thuy Phuong Duyen ◽  
Tran Thi Van ◽  
Nguyen Thu Le Minh ◽  
Nguyen Thi Quynh
Keyword(s):  
Environmental Factors ◽  
Light Intensity ◽  
Leaf Area ◽  
Dark Period ◽  
Dry Weight ◽  
Fresh Weight ◽  
Photoautotrophic Growth ◽  
Temperature Regimes ◽  
Folk Remedy

The arrow leaf abelmoschus rhizome (Hibiscus sagittifolius Kurz), or Sam Bo Chinhin Vietnamese, is a perennial suffrutex herb from which the tuber root is used as a medicine in folk remedy. This species is widely distributed and can be found on many terrains across South East Asia. With an aim to create a large number of uniform and high-quality H. sagittifolius transplants in vitro, effects of some environmental factors such as photoperiod and temperature on the photoautotrophic growth of H. sagittifoliusin vitro plants were investigated. In vitro single nodal cuttings, each with an unfolded leaf, were cultured photoautotrophically (without sucrose and vitamins) on mineral SH medium, under light intensity of 150 µmol m-2 s-1, temperature of 24oC ± 2oC, relative humidity (RH) of 55% ± 5% and three different photoperiods (8, 12 or 16 h d-1) in the first experiment. Commercial polypropylene bags (V = 1,000 ml), attached with two filter-paper membranes, were used as culture vessels. After 42 days of culture, H. sagittifolius plants under the photoperiod of 16 h d-1 had the greatestincreased fresh weight (502.3 mg/plt), increased dry weight (39.5 mg/plt) and leaf area (17.0 cm2) than those put under 8 hd-1or 12 h d-1. In addition, H. sagittifolius plants also showed statistical differences in growth when photoautotrophically cultured in different air temperature regimes, including 28/25oC (photo-/dark period), 25/25oC and 20/25oC. Increased fresh weight (775 mg/plt), increased dry weight (62 mg/plt) and leaf area (22.7 cm2) of in vitro H. sagittifolius plants were the greatest when temperature was maintained at 28 oC during photoperiod. On the contrary, the photoperiod temperature of 20oC resulted in the shortest shoot length and root length of H. sagittifoliusplants. In conclusion, this study proved that H. sagittifolius plants had the best growth when cultured on SH medium, without sucrose and vitamins supplementation, under light intensity of 150 µmol m-2 s-1, RH 55% ± 5%, photoperiod of 16 h d-1, temperature regime of 28/25oC(photo-/dark period). Citation: Nguyen Thuy Phuong Duyen, Tran Thi Van, Nguyen Le Thu Minh, Nguyen Thi Quynh, 2017. Effects of micro-environmental factors on the photoautotrophic growth of Hibiscus sagittifolius Kurz cultured in vitro. Tap chi Sinh hoc, 39(4): 496-506. DOI: 10.15625/0866-7160/v39n4.11030. *Corresponding author: [email protected] 7 September 2017, accepted 15 December 2017

scholarly journals (389) Enhanced Growth in Acclimatization of In Vitro Plantlets of Wasabi japonica using Hydroponics

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1032D-1032
Author(s):  
Joo Hyun Lee ◽  
Yong-Beom Lee ◽  
Kyu Sook Lee
Keyword(s):  
Leaf Area ◽  
Light Availability ◽  
Dry Weight ◽  
Leaf Length ◽  
Superior Performance ◽  
Fresh Weight ◽  
Ps Ii ◽  
Hydroponic System ◽  
Murashige Skoog

Wasabi japonica plantlets were acclimatized in a hydroponic system to determine effective procedures. The plantlets were cultured on solid Murashige-Skoog medium with 3% sucrose. Shoots that formed roots were transplanted into hydroponic systems: 1) acclimatization in ebb-and-flow (EBB) for subirrigation (medium: granulated rockwool and coir); and 2) acclimatization in deep flow technique (DFT). The plantlets were acclimatized for 5 weeks under two irradiance treatments, 50 and 300 mmol·m-2·s-1. Photosynthetic capacity in high PPF was higher than that in low PPF during acclimatization. Electron transport rate from PS II (ETR) and biomass production increased significantly with increased light availability. The fresh weight, dry weight, and leaf area of plantlets in high PPF were higher than those in low PPF. In particular, the dry weight and ETR of the plantlets grown in high PPF increased more than twice as much as those in low PPF. At 50 mmol·m-2·s-1 PPF, growth indexes, such as number of leaves, leaf length, leaf width, leaf area, fresh weight, and dry weight, were higher in EBB (granulated rockwool) > EBB (coir culture) > DFT. At 300 mmol·m-2·s-1 PPF, those indexes were higher in DFT > EBB (granulated rockwool) > EBB (coir). The Wasabi japonica plantlets acclimatized in a hydroponic system also had a superior performance when they were transferred to the field.

scholarly journals CO2 enrichment and increasing light intensity till a threshold level, enhance growth and water use efficiency of lettuce plants in controlled environment

2020 ◽  
Vol 48 (4) ◽  
pp. 2244-2262
Author(s):  
Maryam ESMAILI ◽  
Sasan ALINIAEIFARD ◽  
Mahmoud MASHAL ◽  
Parisa GHORBANZADEH ◽  
Mehdi SEIF ◽  
...  
Keyword(s):  
Light Intensity ◽  
Water Use Efficiency ◽  
Leaf Area ◽  
Water Use ◽  
Dry Weight ◽  
Co2 Concentration ◽  
Photon Flux ◽  
Fresh Weight ◽  
Co2 Concentrations ◽  
Use Efficiency

Carbon dioxide (CO2) and light intensity are the two main environmental drivers known to play important roles in crop growth and yield. In the current study, lettuce seedlings were exposed to four different light intensities [(75, 150, 300 and 600 Photosynthetic Photon Flux Density (PPFD)] and four different concentrations of CO2 (400, 800, 1200 and 1600 ppm). By increasing light intensity and CO2 concentration growth parameters such as fresh weight, dry weight and leaf area were stepwise increased from 75 to 300 PPFD and from 400 ppm to 1200 ppm CO2 concentration. Maximum fresh weight was observed in 300 PPFD under both 1200 ppm and 1600 ppm CO2 concentrations. Highest dry weight was obtained in plants exposed to 300 and 600 PPFD under both 1200 and 1600 ppm CO2 concentrations. Highest leaf area was detected in 300 PPFD under both 1200 and 1600 ppm CO2 concentrations. Widest stomatal pore aperture was detected in 600 PPFD under 400 ppm and 800 ppm CO2 concentrations. Evapotranspiration increased in a light intensity and CO2 concentration-dependent manner; higher light intensity or higher CO2 concentration, more evapotranspiration. Highest water use efficiency (WUE) was achieved in plants exposed to 300 PPFD under 1200 ppm CO2 concentration. In conclusion, to achieve best growth performance and WUE, lettuce should be produced under 300 PPFD light intensity and 1200 ppm CO2.

The effect of growth temperature, level of ammonium nitrate, and light intensity on the growth and nodulation of cowpea (Vigna sinensis endl. ex hassk.)

1965 ◽  
Vol 16 (3) ◽  
pp. 321 ◽  
Author(s):  
PJ Dart ◽  
FV Mercer
Keyword(s):  
Light Intensity ◽  
Ammonium Nitrate ◽  
Primary Root ◽  
Dry Weight ◽  
Optimum Temperature ◽  
Fresh Weight ◽  
Vigna Sinensis ◽  
Root Nodulation ◽  
Temperature Regimes ◽  
Total Dry Weight

The nodulation and growth of cowpea plants (Vigna sinensis Endl. ex Hassk. var. Poona), grown in the CERES phytotron glasshouses, were examined. The plants were grown under six controlled temperature regimes (21, 24, 27, 30, 33, or 36°C day temperature) with ammonium nitrate (NH4N03) additions of 0, 10, 30, and 90 mg nitrogen per pot of six plants; and were grown either under natural daylight or under natural daylight reduced in intensity by one-third by mesh screens. Primary root nodulation was significantly affected by temperature, NH4NO3 level, and light intensity, with an optimum temperature of 24°C. Secondary root nodulation was also affected by light intensity and temperature, but the nodulation pattern (with an optimum temperature of 33°) was almost the inverse of the primary root pattern. Temperature significantly influenced the fresh weight of nodules per plant and the nodule size, with a lesser effect of the NH4NO3 level. Plant dry weight production (tops and roots) was governed by the temperature, NH4NO3 level, and light intensity, the maximum total dry weight being produced at 27°C. Temperature, light intensity, and NH4NO3 level all influenced the plant combined- nitrogen uptake. The nodule leghaemoglobin concentration was significantly reduced at the two extreme temperatures 21° and 36°C. Temperature also markedly affected the nodule structure – particularly the distribution of starch within the nodule.

scholarly journals Effect of Organic Manures on Growth, Rhizome Yield and Quality Attributes of Turmeric (Curcuma longa L.)

2012 ◽  
Vol 10 (1) ◽  
pp. 16-22 ◽  
Author(s):  
M. Z. U. Kamal ◽  
M. N. Yousuf
Keyword(s):  
Leaf Area ◽  
Dry Matter ◽  
Curcuma Longa ◽  
Dry Weight ◽  
Fresh Weight ◽  
Area Index ◽  
Yield Attributes ◽  
Organic Manures ◽  
Neem Cake ◽  
Yield And Quality

The investigation was carried out to evaluate the effect of different organic manures on turmeric with reference to vegetative growth, biomass production, rhizome yield and its attributes of turmeric (Curcuma longa L.). Turmeric showed better response to the application of organic manures. Plant with neem cake application had the taller plant (79.30 cm), maximum number of tillers per plant (5.40), leaf number (5.40), leaf area (44.09) leaf area index (0.429), fresh weight of halum ( 190.05g), fresh weight of root (49.13 g), fresh weight of rhizome per plant (256.21 g) and dry weight of halum (15.21g), dry weight of root (7.32 g), dry weight of rhizome per plant (40.35 g), total dry matter yield (6.85 t ha-1) than those received other types of manures. Moreover, yield attributes such as number of mother rhizomes per plant-1 (1.75), more number of primary rhizomes per plant-1 (5.19), secondary rhizomes per plant-1 (18.03) and tertiary rhizomes per plant (7.69) were also highly accelerated by neem cake application. Similarly, the same treatment expressed the best in terms of size of mother rhizome (7.69 cm), primary rhizome (21.86 cm) and secondary rhizomes (7.05 cm).All these parameters in cumulative contributed to  produce the highest estimated fresh rhizomes yield & cured rhizomes yield (29.48 t ha-1, 5.59 t ha-1 respectively). The highest curing percentage (20.28) was observed in T3 treatment having mustard cake@ 2.0 t/ha. Thus, organic manure like neem cake was best fitted natural fertilizer for turmeric cultivation.DOI: http://dx.doi.org/10.3329/agric.v10i1.11060The Agriculturists 2012; 10(1): 16-22

scholarly journals A method to improve leaf succulence quantification

1999 ◽  
Vol 42 (1) ◽  
Author(s):  
André Mantovani
Keyword(s):  
Leaf Area ◽  
Weight Ratio ◽  
Volume Ratio ◽  
Dry Weight ◽  
Fresh Weight ◽  
Ecological Implications ◽  
Leaf Succulence ◽  
Sun Leaves

Leaf succulence has important physiological and ecological implications. Currently it is quantified by Delf's index (fresh weight/leaf area) and fresh weight/ dry weight ratio. Both indeces are reconsidered and a new index is proposed. Shade and sun leaves from terrestrial, hemiepiphytic and epiphytic aroids were studied. Delf's formula, which does not consider dry weight, overestimated leaf succulence. As fresh weight / dry weight ratio (fw / dw) does not consider leaf area, plants with the same fw / dw ratio were more than five times different in area. The last case was only possible with a decrease in surface / volume ratio and a increase of mesophyll thickness, components not measured by fw / dw ratio. The new index proposed here, which takes into consideration dry weight and leaf area, showed a high correlation to mesophyll thickness.

scholarly journals Silver Nanoparticles Effects on In Vitro Germination, Growth, and Biochemical Activity of Tomato, Radish, and Kale Seedlings

Materials ◽  
2021 ◽  
Vol 14 (18) ◽  
pp. 5340
Author(s):  
Alicja Tymoszuk
Keyword(s):  
Silver Nanoparticles ◽  
Practical Importance ◽  
Plant Cells ◽  
Dry Weight ◽  
Guaiacol Peroxidase ◽  
In Vitro Germination ◽  
Vegetable Crops ◽  
Fresh Weight ◽  
Biochemical Activity

The interactions between nanoparticles and plant cells are still not sufficiently understood, and studies related to this subject are of scientific and practical importance. Silver nanoparticles (AgNPs) are one of the most commonly produced and used nanomaterials. This study aimed to investigate the influence of AgNPs applied at the concentrations of 0, 50, and 100 mg·L−1 during the process of in vitro germination as well as the biometric and biochemical parameters of developed seedlings in three vegetable species: Solanum lycopersicum L. ‘Poranek’, Raphanus sativus L. var. sativus ‘Ramona’, and Brassica oleracea var. sabellica ‘Nero di Toscana’. The application of AgNPs did not affect the germination efficiency; however, diverse results were reported for the growth and biochemical activity of the seedlings, depending on the species tested and the AgNPs concentration. Tomato seedlings treated with nanoparticles, particularly at 100 mg·L−1, had shorter shoots with lower fresh and dry weights and produced roots with lower fresh weight. Simultaneously, at the biochemical level, a decrease in the content of chlorophylls and carotenoids and an increase in the anthocyanins content and guaiacol peroxidase (GPOX) activity were reported. AgNPs-treated radish plants had shorter shoots of higher fresh and dry weight and longer roots with lower fresh weight. Treatment with 50 mg·L−1 and 100 mg·L−1 resulted in the highest and lowest accumulation of chlorophylls and carotenoids in the leaves, respectively; however, seedlings treated with 100 mg·L−1 produced less anthocyanins and polyphenols and exhibited lower GPOX activity. In kale, AgNPs-derived seedlings had a lower content of chlorophylls, carotenoids, and anthocyanins but higher GPOX activity of and were characterized by higher fresh and dry shoot weights and higher heterogeneous biometric parameters of the roots. The results of these experiments may be of great significance for broadening the scope of knowledge on the influence of AgNPs on plant cells and the micropropagation of the vegetable species. Future studies should be aimed at testing lower or even higher concentrations of AgNPs and other NPs and to evaluate the genetic stability of NPs-treated vegetable crops and their yielding efficiency.

scholarly journals PEMANFAATAN PUPUK ORGANIK CAIR PAITAN (Tithonia diversifolia) PADA BERBAGAI DOSIS DAN RAGAM APLIKASINYA TERHADAP PERTUMBUHAN KAILAN (Brassica oleracea Var. Acephala)

2020 ◽  
Vol 11 (1) ◽  
pp. 25
Author(s):  
AHMAD TAOFIK ◽  
SOFIYA HASANI ◽  
AYU CAHYANINGTYAS ◽  
BUDY FRASETYA
Keyword(s):  
Leaf Area ◽  
Plant Height ◽  
Analysis Of Variance ◽  
Growth Parameters ◽  
Organic Fertilizer ◽  
Dry Weight ◽  
Fresh Weight ◽  
Application Method ◽  
Randomized Design ◽  
Two Factors

The paitan plant contains allelopathic compounds for other plants so that the utilize of paitan plant as a source of liquid organic fertilizer (LOF) has not been widely applied. This research aimed to study the interaction between LOF dosage and appropriate application method of LOF to increase the growth of kailan plants. The research conducted at experimental garden of UIN Bandung from June-August 2016. The experimental design was a completely randomized design with two factors and replicated four times. The first factor was application methods (A), i.e. a1=direct application to the soil, and a2= spraying. The second factor was LOF dosage (T), i.e. t0: 0 ml plant-1, t1: 20 ml plant-1, t2: 40 ml plant-1, t3: 60 ml plant-1, t4: 80 ml plant-1. The growth parameters observed i.e. plant height (14, 21, 28, 35 and 42 Day after Transplanting (DAT)), leaf area 42 DAT, plant dry weight 42 DAT, and plant fresh weight 42 DAT. The data then analyzed with analysis of variance at a significant level of 5%. If the analysis of variance was significant, the Duncan multiple range test was used at α=5%. The research results showed there was no interaction between method of application with a various dosage of paitan LOF to all of the growth parameters. The application paitan LOF showed similar growth in terms of plant height, leaf area, plant dry, and plant fresh weight. The application method of paitan LOF direct to the soil or spraying to leaves can not increase kailan plant growth.

In vitro studies on the induction of sporogenous tissue on leaves of cinnamon fern. I. Environmental factors

1972 ◽  
Vol 50 (12) ◽  
pp. 2673-2682 ◽  
Author(s):  
William H. Harvey ◽  
James D. Caponetti
Keyword(s):  
Environmental Factors ◽  
Light Intensity ◽  
Environmental Conditions ◽  
Light Exposure ◽  
Progressive Increase ◽  
Light Phase ◽  
Sporogenous Tissue ◽  
Light Darkness

Intact, set III, cinnamon fern cataphyll and frond primordia, which were shown to have no predisposition to fertility in situ, produced sporangia when excised and cultured under sterile conditions in Knudson's medium supplemented with various levels of sucrose and maintained on 11 different regimens of light, darkness, and temperature for 10 weeks. Increasing levels of sucrose resulted in increased fertility under all environmental conditions, but the highest percentage of fertility was obtained under conditions of continuous dark at 26 °C. As the length of the light phase of the photoperiods decreased, a progressive increase in induction of fertile leaves was observed, suggesting that periods of long light exposure are inhibitory to the initiation of sporangia. Conversely, as the light intensity was increased, an inhibition of sporophyll differentiation occurred. Sporangia excised from dark-induced sporophylls and cultured in the light produced viable spores which germinated yielding haploid gametophytes that ultimately produced sporophytes.

scholarly journals Effects of different levels of mulch and irrigation on growth traits and essential oil content of basil

2020 ◽  
Vol 15 (3) ◽  
Author(s):  
Fatemeh Nejatzadeh-Barandozi
Keyword(s):  
Essential Oil ◽  
Leaf Area ◽  
Wood Chip ◽  
Dry Weight ◽  
Wood Chips ◽  
Growth And Yield ◽  
Oil Yield ◽  
Fresh Weight ◽  
Essential Oil Yield ◽  
Different Levels

Ocimum basilicum L. is an herbaceous plant from the Labiate family that used fresh, as a spice, and as a medicinal plant. Mulch is technique capable of reducing evaporation from the surface of the soil in basil production systems; it has more impacts on yield and essential oil quality of a basil crop. This research was performed in order to study the effects of different mulch types and irrigation regimes on various growth parameters and the essential oil content of basil. Treatments included two mulch types (black plastic, wood chips, and control) and three irrigation levels (100, 80, and 60% of water requirements, calculated by evaporation pan class A), following a randomised complete block design with four replications. Results showed that the effect of irrigation on fresh weight, dry weight, leaf relative water content, leaf area, and essential oil yield was significant (P<0.05). The effect of mulch on fresh weight, dry weight, leaf area, inter-node distance, number of branches, and essential oil yield was significant (P<0.05). Interaction between irrigation and mulch on inter-node distance, fresh weight, dry weight, and leaf area was significant (P<0.05). This study identified wood chips as the best mulch treatment. The highest dry weight yield (38.35 g/plant) and the highest essential oil yield (82.83 L/ha) resulted when wood chip mulch was used in combination with irrigation at 100% of the water requirement. This combination also resulted in the highest water productivity in basil production.   Highlights - Different levels of irrigation alone did not always increase plant growth and yield of basil. - Different levels of mulch alone did not always increase plant growth and yield of basil. - Different levels of irrigation and mulch in combination used, increased, growth and essential oil yield of basil. - This study identified wood chips as the best mulch treatment. - The highest essential oil yield resulted with wood chip mulch and irrigation at 100% of the water requirement.

Comparison of Green Foxtail (Setaria viridis) and Wild Oat (Avena fatua) Growth, Development, and Competitiveness under Three Temperature Regimes

Weed Science ◽  
1993 ◽  
Vol 41 (3) ◽  
pp. 369-378 ◽  
Author(s):  
David A. Wall
Keyword(s):  
Leaf Area ◽  
Dry Weight ◽  
Mature Seed ◽  
Wild Oat ◽  
Replacement Series ◽  
Relative Growth ◽  
Temperature Regimes ◽  
Green Foxtail ◽  
Increasing Temperature ◽  
Series Study

In a replacement series study, barley was more competitive than green foxtail and wild oat at 28/22 and 22/16 C. Wild oat was more competitive with green foxtail at 22/16 C than at 28/22 C. Maximum green foxtail dry weight and leaf area was produced at 28/22 C. As temperature increased, maximum dry weight and leaf area occurred earlier during plant growth. Similarly, maximum wild oat leaf area occurred earlier with increasing temperature, but the greatest leaf area was observed at 16/10 C. Leaf area ratio (LAR) and relative growth rate (RGR) of wild oat did not differ markedly between temperature regimes. Green foxtail LAR was higher while RGR was lower at 16/10 C than at 22/16 or 28/22 C. Under the 16/10 C regime green foxtail produced little mature seed. Greatest seed numbers were produced at 28/22 C. Wild oat produced mature seed under all temperature regimes with the greatest seed numbers produced at 22/16 C.

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