scholarly journals Qualification and quantification of huperzine a from Huperia serrata in Da Lat, Lam Dong province

2016 ◽  
Vol 14 (3) ◽  
pp. 473-478 ◽  
Author(s):  
Vũ Thị Ngọc ◽  
Phạm Thị Hạnh ◽  
Lê Thị Lan Anh ◽  
Nguyễn Tiến Đạt ◽  
Lê Thị Bích Thủy
Keyword(s):  
High Performance ◽  
Elderly Population ◽  
Acetylcholinesterase Inhibitor ◽  
Acetylcholinesterase Activity ◽  
The Elderly ◽  
Hplc Method ◽  
Huperzine A ◽  
Huperzia Serrata ◽  
Layer Chromatography ◽  
Hplc Data

Huperzine A, an alkaloid, was originally isolated from Huperzia serrata. This compound potentially enhances the memory in animal, hence, it has been approved as a drug for the clinical treatment of Alzheimer’s disease, a major disease affecting the elderly population throughout the world. Because Huperzine A is an acetylcholinesterase inhibitor, the presentation of Huperzine A in brain inhibited acetylcholinesterase activity, thus, leading to the increase in concentration of acetylcoline. In Vietnam, H. serrata distributed in Sapa (Lao Cai) and Da Lat (Lam Dong), this species provide valuable pharmaceutical materials to the treatment for Alzheimer’s diseases. In this research, we evaluated the availability of Huperzine A in Huperzia serrata, which was collected from Da Lat (Lam Dong) in two seasons: Spring and Autunm. Thin layer chromatography (TLC) method was used to preliminary qualitative analysis. High performance liquid chromatography (HPLC) method were used for determining Huperzine A content in samples. In the result, Huperzine A is almost existed in leaves of Da Lat Huperzia serrata and equivalent levels of Chinese Huperzia serrata. The Content of Huperzine A was different between two collection samples in Spring and Autumn, by analyze HPLC data, the samples was harvested in Autumn contents 92.5 µg.g-1dry sample and the spring is 75.4 µg.g-1 dry sample. Therefore, the content of Huperzine A in Huperzia serrata’s leaves sample is  harvested in the fall compared with samples collected in the spring is higher 17.1 µg.g-1dry samples.

scholarly journals Isolation of endophytic fungi and screening of Huperzine A–producing fungus from Huperzia serrata in Vietnam

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Thanh Thi Minh Le ◽  
Anh Thi Hong Hoang ◽  
Thuy Thi Bich Le ◽  
Thuy Thi Bich Vo ◽  
Dong Van Quyen ◽  
...  
Keyword(s):  
Endophytic Fungi ◽  
High Performance ◽  
Acetylcholinesterase Inhibitor ◽  
Natural Populations ◽  
Morphological Characteristics ◽  
Huperzine A ◽  
Potato Dextrose Broth ◽  
Huperzia Serrata ◽  
Highly Active ◽  
Production Techniques

Abstract Huperzine A (HupA), a natural Lycopodium alkaloid derived from Huperzia serrata (Thunb. ex Murray) Trev. plants, is a highly active acetylcholinesterase inhibitor and a key compound used for treating Alzheimer’s disease (AD). Recently, HupA has been reported in various endophytic fungi isolated from H. serrata. In the present study, 153 endophytic fungi were isolated from healthy tissues of H. serrata collected from natural populations in Lam Dong province of Central Vietnam. The endophytic fungi were identified based on morphological characteristics and Internal Transcribed Spacer sequences. Among them, 34 strains were classified into seven genera belonging to Ascomycota, including Alternaria, Fusarium, Trichoderma, Penicillium, Paecilomyces, and Phoma, and eight strains belonging to the genus Mucor (Zygomycota). The other strains remained unidentified. According to the results of thin-layer chromatography and high-performance liquid chromatography, only one of the 153 strains, Penicillium sp. LDL4.4, could produce HupA, with a yield 1.38 mg l−1 (168.9 µg g−1 dried mycelium) when cultured in potato dextrose broth, which was considerably higher than that of other reported endophytic fungi. Such a fungus is a promising candidate and alternative to presently available HupA production techniques for treating AD and preventing further memory decline.

scholarly journals In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic Huperzia serrata

Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1112
Author(s):  
Yan Yang ◽  
Liangfang Dai ◽  
Decai Wu ◽  
Limin Dong ◽  
Yisheng Tu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
In Vitro Propagation ◽  
Huperzine A ◽  
Induction Medium ◽  
Naphthalene Acetic Acid ◽  
Regeneration Rate ◽  
Huperzia Serrata ◽  
Chromatography Analysis

Huperzia serrata is a traditional herb and endangered Chinese medicinal material, which has attracted much attention due to its production of Huperzine A (HupA). In vitro propagation of H. serrata is considered a new way to relieve the resource pressure of H. serrata. In this study, three different genotypic wild H. serrata were used for in vitro propagation. Then, the antioxidant activity and the content of HupA in the regenerated H. serrata were investigated. The results showed the survival rate of the explant was increased to 25.37% when using multiple sterilization processes. The best induction medium for H. serrata was the Schenk and Hildebrandt (SH) medium supplemented with 0.5 mg·L−1 Naphthalene acetic acid (NAA) and 0.1 mg·L−1 2,4-Dichlorophenoxyacetic acid (2,4-D), where the regeneration rate of the explant was to 57.04%. The best proliferation medium was the SH medium with NAA (1.0 mg·L−1), as the biomass of in vitro tissue increased 164.17 ± 0.41 times. High-performance liquid chromatography analysis showed that the in vitro culture of three genotypes could produce HupA and the content of HupA was 53.90–87.17 µg·g−1. The antioxidant experiment showed that the methanol extract of in vitro H. serrata had higher antioxidant activity than that of wild H. serrata. This study provides a reliable in vitro H. serrata culture protocol and laid an important foundation for the antioxidant capacity of the thallus and the content of HupA.

scholarly journals Physiologically Active Compounds in Four Species of Phellinus

2017 ◽  
Vol 12 (3) ◽  
pp. 1934578X1701200 ◽  
Author(s):  
Katarzyna Sułkowska-Ziaja ◽  
Anna Maślanka ◽  
Agnieszka Szewczyk ◽  
Bożena Muszyńska
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Total Content ◽  
Dry Weight ◽  
Hplc Method ◽  
Indole Compounds ◽  
Methanolic Extracts ◽  
Free Phenolic Acids ◽  
Layer Chromatography ◽  
Physiologically Active Compounds

The content of two groups of compounds with biological activity (non-hallucinogenic indole compounds and free phenolic acids) were analyzed in extracts of fruiting bodies of four species of Phellinus: P. igniarius, P. pini, P. pomaceus and P. robustus. The presence of indole compounds in methanolic extracts was analyzed by high-performance liquid chromatography and thin-layer chromatography coupled with densitometric detection. Three metabolites (serotonin, tryptamine, and L-tryptophan) were identified. The contents of individual indole compounds ranged from 1.70 (tryptamine in P. robustus) to 8.32 mg x 100 g1 dry weight (L-tryptophan in P. robustus). Four free phenolic acids were detected in methanolic extracts by the HPLC method. The total content ranged from 9.9 mg x 100 g1 DW (P. igniarius) to 32.5 mg x 100 g1 DW (P. robustus).

scholarly journals ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF COMBINATION OF ANTI-ASTHMATIC DRUGS MONTELUKAST AND DOXOFYLLINE

2021 ◽  
Vol 11 (6) ◽  
pp. 86-91
Author(s):  
Sachin N. Kapase
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Method Development ◽  
Analytical Techniques ◽  
Ultra Performance Liquid Chromatography ◽  
Hplc Method ◽  
Uv Spectrophotometry ◽  
Method Development And Validation ◽  
Development And Validation ◽  
Layer Chromatography

For qualitative and quantitative analysis, various analytical techniques are available such as Ultraviolet (UV) Spectrophotometry, High-performance liquid chromatography (HPLC), High-performance thin layer chromatography (HPTLC). As per literature survey, there are some UV, HPLC, Ultra-Performance Liquid Chromatography (UPLC) and HPTLC analytical methods are developed for Montelukast and Doxofylline individually and in a combination with other drugs too, since yet there are no significant stability studies indicating HPLC method reported for Montelukast and Doxofylline combinations. In the current study, the HPLC method is developed and validated for simultaneous quantitative estimations of Montelukast and Doxofylline. These present techniques are more efficient and sensitive as compared to other analytical techniques.

scholarly journals Simultaneous Estimation of Pantoprazole and Domperidone in Pure Powder and a Pharmaceutical Formulation by High-Perfomance Liquid Chromatography and High-Performance Thin-Layer Chromatography Methods

2007 ◽  
Vol 90 (1) ◽  
pp. 142-146 ◽  
Author(s):  
Bhavesh H Patel ◽  
Bhanubhai N Suhagia ◽  
Madhabhai M Patel ◽  
Jignesh R Patel
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Hplc Method ◽  
Uv Detection ◽  
Simultaneous Estimation ◽  
Phase Quantification ◽  
Layer Chromatography

Abstract This paper describes validated high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) methods for the simultaneous estimation of pantoprazole (PANT) and domperidone (DOM) in pure powder and capsule formulations. The HPLC separation was achieved on a Phenomenex C18 column (250 mm id, 4.6 mm, 5 μm) using 0.01 M, 6.5 pH ammonium acetate buffer-methanol-acetonitrile (30 + 40 + 30, v/v/v, pH 7.20) as the mobile phase at a flow rate of 1.0 mL/min at ambient temperature. The HPTLC separation was achieved on an aluminum-backed layer of silica gel 60F254 using ethyl acetatemethanol (60 + 40, v/v) as the mobile phase. Quantification was achieved with ultraviolet (UV) detection at 287 nm over the concentration range 400-4000 and 300-3000 ng/mL with mean recovery of 99.35 ± 0.80 and 99.08 ± 0.57% for PANT and DOM, respectively (HPLC method). Quantification was achieved with UV detection at 287 nm over the concentration range 80-240 and 60-180 ng/spot with mean recovery of 98.40 ± 0.67 and 98.75 ± 0.71% for PANT and DOM, respectively (HPTLC method). These methods are simple, precise, and sensitive, and they are applicable for the simultaneous determination of PANT and DOM in pure powder and capsule formulations.

scholarly journals Investigation of Experimental and In Silico Physicochemical Properties of Thiazole-Pyridinium Anti-Acetylcholinesterase Derivatives with Potential Anti-Alzheimer’s Activity

2020 ◽  
Vol 27 (3) ◽  
pp. 366-377
Author(s):  
Golaleh Ghotbi ◽  
Maryam Hamzeh-Mivehroud ◽  
Arezou Taghvimi ◽  
Soodabeh Davaran ◽  
Siavoush Dastmalchi
Keyword(s):  
Physicochemical Properties ◽  
In Silico ◽  
High Performance ◽  
Acetylcholinesterase Activity ◽  
Log P ◽  
Target Tissue ◽  
In Silico Studies ◽  
Chromatographic Condition ◽  
Rf Values ◽  
Layer Chromatography

Background: Physicochemical properties play important role in fundamental issues like absorption and distribution of pharmaceuticals to the target tissue. This is particularly importantfor drugs acting in central nervous system (CNS). In this study, physicochemical properties of previously synthesized thiazole-pyridinium derivatives with anti-acetylcholinesterase activity and possible anti-Alzheimer effect were studied. Methods: Partition coefficient (n-octanol/water) and chromatographic Rf values for the studied compounds were determined using shake flask and high performance thin layer chromatography(HPTLC) methods, respectively. Different druglikeness properties of the compounds were also calculated using available software and web-servers. Results: The experimentally determined logarithm of partition coefficients (log P) for the studied compounds were in the range of -1.00 to -0.38. The Rf values for the studied compounds under the applied chromatographic condition ranged between 0.38 to 0.58. Moreover, calculated physicochemical properties, and druglikeness scores of the studied thiazole-pyridiniumderivatives and matching piperidine analogues were predicted. Furthermore, some ADMETfeatures of studied compounds like toxicity and metabolism by CYP450 (2C9, 2D6, 3A4, 1A2and 2C19) enzymes were predicted. Conclusion: The ranges of experimental and calculated LogP values for the studied thiazolepyridinumswere close. However, the determined Rf values showed relatively better correlation to the predicted LogP values indicating the suitability of used chromatographic method for comparing the lipophilicity of the positively charged pyridinium derivatives. The studied compounds were predicted to pass GI membrane and reach the CNS where they can exert their effects. In silico studies indicate that the piperidine counterparts of the studied thiazolepyridiniumsmay represent anti-Alzheimer agents with improved druglikeness properties.

scholarly journals Determination of flavones in species of Thymus L. (Lamiaceae) from Macedonian flora

2001 ◽  
Vol 47 ◽  
pp. 9-14
Author(s):  
Svetlana Kulevanova ◽  
Marina Stefova ◽  
Tatjana Kadifkova Panovska ◽  
Jasmina Tonic ◽  
Trajce Stafilov
Keyword(s):  
Ethyl Acetate ◽  
High Performance ◽  
Gradient Elution ◽  
Hplc Method ◽  
Total Flavonoids ◽  
Column Temperature ◽  
Plant Samples ◽  
Layer Chromatography ◽  
Hydrolysis Of

Assay of flavonoids in extracts of seven Thymus L. (Lamiaceae) species from Macedonia including identification and quantification was performed. Extracts obtained after hydrolysis of air dried samples (A1) were analyzed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). Luteolin and apigenin were identified in comparison to authentic standard substances. The content of total flavonoids in plant samples determined by UV-Vis spectrometry (with AlCl3) ranged from 0.05-0.13 %. Two other extracts were prepared by extraction with a mixture of ethanol:water (7:3, V/V), evaporation until only water remained and extraction first with diethylether (A2) and secondly with ethyl acetate (A3). The content of flavonoids in diethyl-ether and ethyl acetate extracts ranged from 52.5-244.4 mg·ml-1 and 48.7 -117.5 mg·ml-1, respectively. For quantification of luteolin and total flavonoids the HPLC method was applied, using reverse phase column C18, mobile phase consisting of 5% acetic acid and methanol in gradient elution mode and column temperature set to 40 o C. The content of luteolin in the plant samples ranged from 0.23-0.48 % (m/m), while the content of total flavonoids was found to be 0.26-0.52 %.

scholarly journals Systematic and Model-Assisted Process Design for the Extraction and Purification of Artemisinin from Artemisia annua L.—Part III: Chromatographic Purification

Processes ◽  
2018 ◽  
Vol 6 (10) ◽  
pp. 180 ◽  
Author(s):  
Fabian Mestmäcker ◽  
Axel Schmidt ◽  
Maximilian Huter ◽  
Maximilian Sixt ◽  
Jochen Strube
Keyword(s):  
High Performance ◽  
Artemisia Annua ◽  
Batch Process ◽  
Pilot Scale ◽  
Hplc Method ◽  
Artemisia Annua L ◽  
Reproducibility Study ◽  
Extraction And Purification ◽  
Layer Chromatography ◽  
Characteristic Process

In this study, the purification of an extract from Artemisia annua L. using chromatographic methods is studied. In a first step, a screening of different phases and solvents using thin-layer chromatography (TLC) was performed. Then, a laboratory-scale high performance liquid chromatography (HPLC) method was developed and transferred to a pilot scale. A reproducibility study based on 120 injections was carried out. The batch process that was developed and the results from a designed continuous simulated moving bed (SMB) chromatography were compared based on characteristic process numbers and economy.

scholarly journals Chemical Differentiation of Dendrobium officinale and Dendrobium devonianum by Using HPLC Fingerprints, HPLC-ESI-MS, and HPTLC Analyses

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Zi Ye ◽  
Jia-Rong Dai ◽  
Cheng-Gang Zhang ◽  
Ye Lu ◽  
Lei-Lei Wu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance ◽  
Hplc Method ◽  
Dendrobium Officinale ◽  
Chemical Markers ◽  
Esi Ms ◽  
Medicinal Value ◽  
Limited Supply ◽  
Layer Chromatography ◽  
Hptlc Method

The stems of Dendrobium officinale Kimura et Migo (Dendrobii Officinalis Caulis) have a high medicinal value as a traditional Chinese medicine (TCM). Because of the limited supply, D. officinale is a high priced TCM, and therefore adulterants are commonly found in the herbal market. The dried stems of a closely related Dendrobium species, Dendrobium devonianum Paxt., are commonly used as the substitute; however, there is no effective method to distinguish the two Dendrobium species. Here, a high performance liquid chromatography (HPLC) method was successfully developed and applied to differentiate D. officinale and D. devonianum by comparing the chromatograms according to the characteristic peaks. A HPLC coupled with electrospray ionization multistage mass spectrometry (HPLC-ESI-MS) method was further applied for structural elucidation of 15 flavonoids, 5 phenolic acids, and 1 lignan in D. officinale. Among these flavonoids, 4 flavonoid C-glycosides were firstly reported in D. officinale, and violanthin and isoviolanthin were identified to be specific for D. officinale compared with D. devonianum. Then, two representative components were used as chemical markers. A rapid and reliable high performance thin layer chromatography (HPTLC) method was applied in distinguishing D. officinale from D. devonianum. The results of this work have demonstrated that these developed analytical methods can be used to discriminate D. officinale and D. devonianum effectively and conveniently.

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