scholarly journals FECAL CONTAMINATION AND THE PROPORTION OF HUMAN-ASSOCIATED E. coli ALONG NHUE RIVER, VIET NAM

2018 ◽  
Vol 56 (2C) ◽  
pp. 23-29
Author(s):  
Mai Tanaka
Keyword(s):  
Microbial Contamination ◽  
Domestic Wastewater ◽  
Wastewater Discharge ◽  
Asian Countries ◽  
Rapid Urbanization ◽  
Potential Health ◽  
E Coli ◽  
River Water Samples ◽  
Positive Proportion ◽  
Middle Stream

According to rapid urbanization in Asian countries, the amount of domestic wastewater discharge has been increasing, resulted in water pollution and potential health risk on human. To secure biological safety of rivers, it is critical to understand the sources of contamination. The present study applied the human-associated E.coli genetic marker (H8) to characterize the source of microbial contamination. E. coli concentration and other pollutants indices were investigated at 10 sites along the river: S1 (upstream) – S10 (downstream). For 220 E. coli isolates collected from river water samples, Real-Time PCR was performed with H8. The results showed that organic pollutants peaked at the middle stream, although they were originally low at upstream (S1). The positive proportion of H8 with E. coli peaked at 40.9 % at middle stream (S6), which was significantly higher than S1 (4.5 %) (p = 0.012). This high proportion implied the relative dominance of human-associated E. coli, which were in line with a large inflow of sanitary wastewater in middle stream, indicated by land use along the river. Thus, we successfully demonstrated the usefulness of H8 to track the source of microbial contamination in the river. 

Microbial impact assessment of the 2018 European drought on groundwater quality in the Republic of Ireland: An opportunistic field study

2020 ◽  
Author(s):  
Jean O'Dwyer ◽  
John Weatherill ◽  
Carlos Chique ◽  
Paul Hynds
Keyword(s):  
Microbial Contamination ◽  
Domestic Wastewater ◽  
Extreme Weather Events ◽  
E Coli ◽  
Weather Events ◽  
Domestic Wastewater Treatment ◽  
Drought Conditions ◽  
The Republic ◽  
Private Wells ◽  
Wastewater Treatment Systems

<p>Global climate change models predict an increase in both the frequency and severity of extreme weather events, including prolonged drought conditions, thus posing a unique set of challenges for regions traditionally unaccustomed to severe climate phenomena. This is particularly significant for the occurrence of severe drought events in areas characterised by temperate maritime climates, such as the Republic of Ireland (ROI). While numerous studies have explored the impacts of drought on groundwater levels and chemical contamination, few studies have sought to investigate the impacts of sporadic drought events on the microbial quality of groundwater for human consumption. Accordingly, the 2018 (June-August) European drought event represented a unique opportunity to investigate the effects of prolonged low rainfall and elevated temperature (relative to seasonal means) on the incidence of faecal indicator organisms (FIOs) among unregulated domestic groundwater supplies in the ROI.</p><p>A dual-sampling fieldwork regime (during and post-event) of private wells (n=74) and subsequent risk factor (logistic regression) and bivariate analyses were used to evaluate the potential role of meteorological and site specific (hydrogeology, contaminant sources etc.) conditions on the incidence of microbial contamination. During absolute drought conditions (≥15 days characterised by no measureable precipitation, June 2018), the sampled cohort exhibited a significantly decreased risk of microbial contamination (OR: 0.356, p = 0.024) with 12.2% (n = 9/74) of supplies contaminated with Escherichia coli (E. coli), increasing to 28.4% (n = 21/74) upon abatement of drought conditions (October 2018). No analysed risk factors were associated with E. coli presence at the 95% confidence level, although, the presence of onsite domestic wastewater treatment systems (U = 1.03 p = 0.057) approached statistical significance during the drought. Findings suggest that the 2018 European drought served to decrease background levels of FIO within private wells in the ROI, likely due to reduced hydraulic loading from the surface, soil moisture deficits and consequently, significantly decreased bacterial survival. Results would seem to reiterate the significance of onsite domestic wastewater treatment systems as a source of subsurface contaminants in Ireland. The presented opportunistic field study provides a critical characterization of the impacts of unprecedented drought events on microbiological water quality in domestic groundwater supplies in temperate regions, and may be used by sanitary/environmental engineers, hydrologists, hydrogeologists, policy-makers, planners and healthcare practitioners to safeguard against the future human health effects of climate change and extreme weather events.   </p>

Occurrences of Giardia cysts in beach water

1998 ◽  
Vol 38 (12) ◽  
pp. 73-76 ◽  
Author(s):  
B. S. W. Ho ◽  
T.-Y Tam
Keyword(s):  
Health Risk ◽  
Water Sample ◽  
Water Samples ◽  
High Concentration ◽  
Potential Health ◽  
Infectious Dose ◽  
Potential Health Risk ◽  
E Coli ◽  
Beach Water ◽  
Giardia Cysts

A total of 64 beach water samples with various bacteriological quality (Grades 1 to 4) were analysed for their bacteriological and parasitological contents (E coli and Giardia cysts respectively). Results indicated that Giardia cysts were detected in less than 10% of the Grade 1 beach water samples with E coli concentrations of <24/100mL. For Grades 2, 3 & 4 beach water samples, Giardia cysts were found, respectively, in 85, 50 and 64% of the samples. Except for one beach water sample which had an unusually high concentration of Giardia cysts (23 cysts/L), they were generally present at moderate concentrations (<10 cysts/L) in all other beach water samples. Despite moderate levels of Giardia cysts present in beach water of different grades, the potential health risk faced by swimmers bathing in local beach water needs to be carefully assessed as Giardia is known to have a low infectious dose.

scholarly journals Effects of Urban Forms on Separate Drainage Systems: A Virtual City Perspective

Water ◽  
2019 ◽  
Vol 11 (4) ◽  
pp. 758 ◽  
Author(s):  
Jia ◽  
Sitzenfrei ◽  
Rauch ◽  
Liang ◽  
Liu
Keyword(s):  
Urban Form ◽  
Domestic Wastewater ◽  
Cost Effective ◽  
Three Dimensions ◽  
Rapid Urbanization ◽  
Drainage Systems ◽  
Urban Forms ◽  
Storm Water Management Model ◽  
Wastewater Systems ◽  
Performance Dimensions

The development of urban drainage systems is challenged by rapid urbanization; however, little attention is paid to the urban form and its effects on these systems. This study develops an integrated city-drainage model that configures typical urban forms and their associated drainage infrastructures, specifically domestic wastewater and rainwater systems, to analyze the relationship between them. Three typical types of urban forms were investigated: the square, the star, and the strip. Virtual cities were designed first, with the corresponding drainage systems generated automatically and then linked to a model herein called the Storm Water Management Model (SWMM). Evaluation was based on 200 random configurations of wastewater/rainwater systems with different structures or attributes. The results show that urban forms play more important roles on three dimensions of performance, namely economic efficiency, effectiveness, and adaptability, of the rainwater systems than of the wastewater systems. Cost is positively correlated to the effectiveness of rainwater systems among the different urban forms, while adaptability is negatively correlated to the other two performance dimensions. Regardless of the form, it is difficult for a city to make its drainage systems simultaneously cost-effective, efficient, and adaptable based on the virtual cities we investigated. This study could inspire the urban planning of both built-up and to-be-built areas to become more sustainable with their drainage infrastructure by recognizing the pros and cons of different macroscale urban forms.

English Education Reform in Asian Countries

2021 ◽  
Author(s):  
Wenyang Sun ◽  
Xue Lan Rong
Keyword(s):  
Education Reform ◽  
English Language ◽  
English Education ◽  
Language Education ◽  
Asian Countries ◽  
Education Policies ◽  
English Language Education ◽  
Rapid Urbanization ◽  
The Status ◽  
Globalized World

Language education is becoming an increasingly important topic in education in Asian countries, especially as schools in Asian countries have become more multilingual and multicultural as a result of rapid urbanization and globalization. A comparative analysis of the issues in language education reform in Asian countries—using China, India, Japan, South Korea, and Singapore as examples—shows that, historically and currently, English language education policies are shaped by various underpinning ideologies such as linguicism, nationalism, and neoliberalism. English can serve as a vehicle for upward socioeconomic mobility, or an instrument of linguistic imperialism, or both, in Asia contexts. These ideologies, through language education policies and reforms, impact the status as well as the pedagogy and promotion of the English language. There is a trend and a need with regard to addressing critical consciousness in English education in order to counter the forces of linguicism and neoliberalism in an increasingly multilingual, multicultural, and globalized world.

Potential Public Health Significance of Non-Escherichia coli Coliforms in Food

1979 ◽  
Vol 42 (2) ◽  
pp. 161-163 ◽  
Author(s):  
ROBERT M. TWEDT ◽  
BRENDA K. BOUTIN
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Diarrheal Disease ◽  
Health Hazard ◽  
High Yield ◽  
Potential Health ◽  
E Coli ◽  
Potential Health Hazard ◽  
Health Significance

Several coliform species other than Escherichia coli are often associated with and possibly responsible for acute and chronic diarrheal disease. Recent evidence suggests that non-Escherichia coli coliforms may be capable of colonizing the human intestine and producing enterotoxin(s) in high-yield. Whether these organisms are newly capable of causing disease because of infestation with extrachromosomal factors mediating pathogenicity or simply because of inherent pathogenic capabilities that have gone unrecognized, they pose a potential health hazard. Food, medical, and public health microbiologists should be aware that the non-E. coli coliforms contaminating foods may be potential enteropathogens. This possibility may make determination of their pathogenic capabilities even more important than identification of their taxonomic characteristics.

scholarly journals Identification and management of microbial contaminations in a surface drinking water source

2007 ◽  
Vol 5 (S1) ◽  
pp. 67-79 ◽  
Author(s):  
J. Åström ◽  
T. J. R. Pettersson ◽  
T. A. Stenström
Keyword(s):  
Drinking Water ◽  
Water Intake ◽  
Surface Waters ◽  
Microbial Contamination ◽  
Water Source ◽  
Drinking Water Source ◽  
Raw Water ◽  
Indicator Organisms ◽  
E Coli ◽  
The City

Microbial contamination of surface waters constitutes a health risk for drinking water consumers which may be lowered by closing the raw water intake. We have evaluated microbial discharge events reported in the river Göta älv, which is used for raw water supply to the city of Göteborg. Elevated levels of faecal indicator bacteria were observed during periods of closed raw water intake. High bacteria levels were, however, also occasionally detected during periods of open intake, probably as a result of microbial discharge far upstream in the river which may be difficult to predict and manage by closing the intake. Accumulated upstream precipitations, resulting in surface runoff and wastewater contaminations in the catchment, correlated positively with the levels of total coliforms, E. coli, intestinal enterococci and sulfite-reducing clostridia. Levels of faecal indicator organisms were negatively correlated to the water temperature due to enhanced survival at lower temperatures. Wastewater discharges from a municipality located just upstream of the water intake resulted in elevated E. coli concentrations downstream at the raw water intake for Göteborg. To improve the prediction of microbial contaminations within the river Göta älv, monitoring data on turbidity and upstream precipitation are of particular importance.

Microbial Survey of Selected Ontario-Grown Fresh Fruits and Vegetables

2007 ◽  
Vol 70 (12) ◽  
pp. 2864-2867 ◽  
Author(s):  
LINDSAY ARTHUR ◽  
SANDRA JONES ◽  
MARTHA FABRI ◽  
JOSEPH ODUMERU
Keyword(s):  
Food Safety ◽  
Media Coverage ◽  
Microbial Contamination ◽  
Fruits And Vegetables ◽  
Risk Mitigation ◽  
Fresh Market ◽  
E Coli ◽  
Safety Research ◽  
Potential Risks ◽  
Leaf Lettuce

Recent produce-related outbreaks have been receiving heightened media coverage, which has increased public concern toward the safety of fresh fruits and vegetables. In response, the microbial contamination of Ontario-grown fresh fruits and vegetables was evaluated by the Ontario Ministry of Agriculture, Food and Rural Affairs during the summer of 2004. Prior to this survey, information specific to the microbial contamination of Ontario-produced fruits and vegetables was limited. This nonregulatory survey had two objectives: (i) to obtain a general microbiological profile of selected fruits and vegetables produced in Ontario and (ii) to use the information and knowledge gained from this survey to direct and support future on-farm food safety research and food safety programs to manage potential risks. In all, 1,183 samples, including muskmelon (151), scallions and green onions (173), leaf lettuce (263), organic leaf lettuce (112), head lettuce (155), parsley (127), cilantro (61), and fresh market tomatoes (141), were collected and analyzed. Samples were analyzed for Salmonella, Shigella, and generic E. coli. Enrichment cultures positive for E. coli were further assessed for verotoxigenicity. One sample each of Roma tomato and organic leaf lettuce were positive for Salmonella, with no samples yielding Shigella or verotoxigenic E. coli. The E. coli prevalence was highest in parsley (13.4%), followed by organic leaf lettuce (11.6%), leaf lettuce (6.5%), scallions (6.4%), cilantro (4.9%), muskmelon (1.3%), head lettuce (0%), and fresh market tomatoes (0%). These findings, in combination with foodborne illness data, will help target those commodities that require more focused risk mitigation efforts.

scholarly journals PENGOLAHAN LIMBAH DOMESTIK RUMAH MAKAN DENGAN PROSES MOVING BED BIOFILM REACTOR (MBBR)

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Aulia Ulfah Farahdiba ◽  
Yayok Suryo Purnomo ◽  
Satria Nugraha Sakti ◽  
Muhammad Firdaus Kamal
Keyword(s):  
Domestic Wastewater ◽  
Biofilm Reactor ◽  
Water Tank ◽  
Wastewater Discharge ◽  
Moving Bed Biofilm Reactor ◽  
Domestic Waste ◽  
Moving Bed ◽  
Study Results ◽  
The Media ◽  
Media Volume

Semakin banyaknya usaha rumah makan, maka air limbah yang dihasilkan akan bertambah dan akan menjadi suatu permasalahan. Pada proses pengolahan limbah khususnya yang mengandung senyawa organik, salah satu teknologi yang digunakan adalah dengan menggunakan proses Moving Bed Bioflm Reactor (MBBR). Pada prinsipnya, MBBR merupakan proses lumpur aktif yang ditingkatkan dengan menambahkan media (carrier) ke dalam reaktor aerasi. Penelitian ini bertujuan menganalisis penurunan zat organik COD dan TSS dengan menggunakan proses MBBR pada limbah domestik rumah makan. Pada penelitian ini media yang digunakan adalah media kaldness 1 (K1) dengan variasi volume media yaitu tanpa media, 15%, 30%, 45%, dan 60%, serta variasi debit aliran air limbah yang akan masuk ke dalam reaktor MBBR sebesar 15 ml/menit, 30 ml/menit, 45 ml/menit, 60 ml/menit, dan 75 ml/menit. Hasil penelitian ini menunjukan bahwa kemampuan penyisihan beban pencemar COD dari limbah air domestik rumah makan oleh reaktor MBBR dengan volume media 60% dan debit 15 ml/menit sangat efektif. Reaktor ini mampu menurunkan nilai COD hingga 97,73% dan untuk TSS hanya sebesar 63,04% karena pada penelitian ini tidak menggunakan bak pengendap akhir sehingga nilai penyisihan TSS tidak terlalu besar.  Kata kunci:  COD, kaldness 1, moving bed bioflm reactor, TSS The more business restaurant, wastewater will be produced. In the processing of wastewater which contains an organic compound the most technology that is used is microorganisms or biological process by using Moving Bed Biofilm Reactor (MBBR). In principle, MBBR is a process of activated sludge is improved by adding carrier into aeration reactor. This study aims to analyze the decrease in organic matter COD and TSS by using the MBBR process in restaurant domestic wastewater. The media that is used in this study is Kaldnes 1 (K1) with variation media volume that is without media, 15%, 30%, 45%, and 60%, and then the flow variation of wastewater discharge that will enter into MBBR reactor 75 ml/minute, 60 ml/minute, 45 ml/minute, 30 ml/minute, and 15 ml/minute. The results of this study results that the ability to set aside COD waste from restaurant domestic waste by MBBR with media volume 60% with 15 ml/minute debit more effective. This reactor able to increase COD till 97,73% and for TSS just 63,04% in this study do not use water tank so the TSS value is not too big. Keywords: COD, kaldness 1, moving bed bioflm reactor, TSS

scholarly journals Microbial quality of beef and hygiene practices in small and medium slaughterhouses and butcheries in Uganda

2020 ◽  
Author(s):  
Juliet Kyayesimira ◽  
Wangalwa Rapheal ◽  
Grace Kagoro Rugunda ◽  
Lejju Julius Bunny ◽  
Morgan Andama ◽  
...  
Keyword(s):  
Microbial Contamination ◽  
Microbiological Quality ◽  
Microbial Load ◽  
Microbial Quality ◽  
E Coli ◽  
Hygiene Practices ◽  
Safe Level ◽  
Microbiological Methods ◽  
Beef Carcass

Abstract Background If hygiene practices along the beef processing nodes at small and medium enterprise (SME) slaughter houses and butcheries are not observed, they may pose a health risk due to microbial contamination. In SME slaughterhouses and butcheries, the risk may be higher due to transmission of foodborne pathogens. This study determined the hygienic practices and microbial quality risk among meat handlers (MH) in SME slaughterhouses and butcheries. Methods Assessment of microbiological quality of beef was carried out at slaughter houses and butcher shops in the districts of Western, Central and Eastern regions of Uganda. A cross sectional study was conducted from June 2017 to January 2018 using observation checklists to record unhygienic practices among the various actors. Microbial load at slaughter and butchery was determined from a total of 317 swab samples collected from carcass, tools, protective clothing and hands of meat handlers. The microbiological quality of beef was evaluated using standard microbiological methods. The samples were inoculated into differential and selective media. Results Butcheries had the highest microbial load on beef carcass ranging from 4.76 log 10 cfu/cm 2 to 7.90 log 10 cfu/cm 2 Total Viable Counts (TVC) while Total Coliform Counts (TCC) ranged from 1.42 log 10 cfu/cm 2 to 3.05 log 10 cfu/cm 2 , E. coli ranged from 0.68 log 10 cfu/cm 2 to 1.06 log 10 cfu/cm 2 and Staphylococcus aureus ranged from 3.25 log 10 cfu/cm 2 to 4.84 log 10 cfu/cm 2 . Salmonella was absent in all the samples analysed. Results of overall microbial quality of beef in Uganda indicated that only TCC (1.60±0.26 log 10 cfu/cm 2 ) of the beef carcass samples at slaughter houses was not significantly above the safe level (p = 0.693). Overall microbial load (TVC, TCC, E. coli and S. aureus ) at butcheries were significantly (p < 0.05) above the safe level. Butcheries of Mbale district had the highest percentage (70%) of beef carcass samples above the TCC safe levels whereas butcheries of Mbarara district had the highest percentage (40%) of beef carcass samples above the E. coli safe levels. TVC from hands and clothes at butchery across the three study districts varied significantly (p=0.007) with the highest counts (7.23 log 10 cfu/cm 2 ) recorded from personnel clothes and lowest (5.46 log 10 cfu/cm 2 ) recorded from hands. On the other hand, swab samples picked from chopping board and working table at the butchery did not show significant variation in TVC, TCC, E. coli and S. aureus microbial loads across the three study districts. Conclusion Hygienic handling of carcasses after slaughter is critical in preventing contamination and ensuring meat safety in informal meat trading sectors in Uganda. Handling practices of beef at Ugandan slaughterhouses and butcheries are not hygienic hence not up to standard and they contribute to microbial contamination of beef posing a risk to consumers. The distribution stage is the most critical period, during which the quality of meat can easily be compromised.

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